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Protein

DNA-directed RNA polymerase III subunit rpc2

Gene

polr3b

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and rpc2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1557ZincBy similarity1
Metal bindingi1560ZincBy similarity1
Metal bindingi1569ZincBy similarity1
Metal bindingi1572ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1557 – 1572C4-typeAdd BLAST16

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit rpc2 (EC:2.7.7.6)
Short name:
RNA polymerase III subunit C2
Alternative name(s):
DNA-directed RNA polymerase III subunit B
Cleaved into the following chain:
Gene namesi
Name:polr3b
Synonyms:rpc2
ORF Names:DDB_G0288449
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288449 rpc2

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003280681 – 505DNA-directed RNA polymerase III subunit rpc2, 1st partSequence analysisAdd BLAST505
ChainiPRO_0000328069506 – 969Ddi rpc2 inteinSequence analysisAdd BLAST464
ChainiPRO_0000328070970 – 1608DNA-directed RNA polymerase III subunit rpc2, 2nd partSequence analysisAdd BLAST639

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.Curated

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PaxDbiQ54IZ9

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex.

Protein-protein interaction databases

STRINGi44689.DDB0216313

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi612 – 624Poly-AsnAdd BLAST13
Compositional biasi720 – 726Poly-Gln7
Compositional biasi731 – 737Poly-Gln7
Compositional biasi899 – 907Poly-Asn9

Sequence similaritiesi

Belongs to the RNA polymerase beta chain family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1557 – 1572C4-typeAdd BLAST16

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0215 Eukaryota
COG0085 LUCA
InParanoidiQ54IZ9
KOiK03021
OMAiMLCTSDT

Family and domain databases

CDDicd00653 RNA_pol_B_RPB2, 1 hit
Gene3Di2.40.270.10, 3 hits
2.40.50.150, 2 hits
3.90.1110.10, 1 hit
InterProiView protein in InterPro
IPR015712 DNA-dir_RNA_pol_su2
IPR007120 DNA-dir_RNAP_su2_dom
IPR037033 DNA-dir_RNAP_su2_hyb_sf
IPR003587 Hint_dom_N
IPR036844 Hint_dom_sf
IPR030934 Intein_C
IPR006141 Intein_N
IPR007121 RNA_pol_bsu_CS
IPR007644 RNA_pol_bsu_protrusion
IPR007642 RNA_pol_Rpb2_2
IPR037034 RNA_pol_Rpb2_2_sf
IPR007645 RNA_pol_Rpb2_3
IPR007646 RNA_pol_Rpb2_4
IPR007647 RNA_pol_Rpb2_5
IPR007641 RNA_pol_Rpb2_7
IPR014724 RNA_pol_RPB2_OB-fold
PANTHERiPTHR20856 PTHR20856, 1 hit
PfamiView protein in Pfam
PF04563 RNA_pol_Rpb2_1, 1 hit
PF04561 RNA_pol_Rpb2_2, 1 hit
PF04565 RNA_pol_Rpb2_3, 1 hit
PF04566 RNA_pol_Rpb2_4, 1 hit
PF04567 RNA_pol_Rpb2_5, 1 hit
PF00562 RNA_pol_Rpb2_6, 1 hit
PF04560 RNA_pol_Rpb2_7, 1 hit
SMARTiView protein in SMART
SM00306 HintN, 1 hit
SUPFAMiSSF51294 SSF51294, 2 hits
TIGRFAMsiTIGR01443 intein_Cterm, 1 hit
TIGR01445 intein_Nterm, 1 hit
PROSITEiView protein in PROSITE
PS50818 INTEIN_C_TER, 1 hit
PS50817 INTEIN_N_TER, 1 hit
PS01166 RNA_POL_BETA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54IZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPESVGYCN NPDFLHKGSM DELYNISKLT DDIKPVEEKW KLVPAFMKCR
60 70 80 90 100
GLVKQHIDSF NFFINVEMKK IVKANERLTA ENDPSYFVRF TDINVGSPTS
110 120 130 140 150
TEDNLDSVQL TPQRCRLRDM TYSAPIFVNI EYTRNKQIIS KRDVHIGNIP
160 170 180 190 200
IMLRSSNCVL SKKTPEQMAA LGECPMDPGG YFIVRGQEKV ILNHEQLSKN
210 220 230 240 250
RIIIEMDSKG LPSASVTSST HERKSRTGVT LKNEKLYLKH NTFGEDIPVA
260 270 280 290 300
IVLKGMGVET DQEMAQLVGS DDVFLNAITP SLEECQKCGV HTAAQALDYL
310 320 330 340 350
GSRIKVFRRP YGVQNKKTKS EEARDILAGV VLNHVPVRRY NFRLKVIYLS
360 370 380 390 400
LMIRRIIMAS KDKSCLDDKD YYGNKRIELS GQLISLLFED CFKKFQSELK
410 420 430 440 450
KSVDQAIAKA NRAENLDLPK LIRTDTITNG FTHAISSGQW NLKRFRMERS
460 470 480 490 500
GVSQVLSRLS YISCMGMMTR IQSQFEKTRK VAGPRSLQPS QWGMLCPSDT
510 520 530 540 550
PEGEACLHPD TIITMSNGQQ KPIRQLKDGD SIITLDPITM EAHSTRIYSH
560 570 580 590 600
FIKSSSQYGK QLLKITTITG KEIICTNDHR FLTSNGNWKQ SKDLLLNDKL
610 620 630 640 650
FLISSSNQLE FNNNNNENNN ENNNDIIEIL NENQLINQGV VPIKIIQELK
660 670 680 690 700
SIELLPLLNN NEKLITISRI IGSIDKIGSN KQNEPIIQYQ FNLISDFDQF
710 720 730 740 750
LKDLQYLGFI NPIYKLNEEQ QQQQQQKIDH QQQQQQQVEQ QQKSIIIDFI
760 770 780 790 800
GSSFGYFIQS LLNEKNWIEK SNNQFVKKEF LSSFISNVNR IQFNIIEEIN
810 820 830 840 850
QQNNDSNFKI LLNYKQQKQQ QNEKEERAVV DHDNEIFNIK SLQILLNQFN
860 870 880 890 900
VISSIDFEES NLIIINSSLK KFIDLINIKF NQKLNNQIIQ IREYLNYINY
910 920 930 940 950
NNNNNNNEEI NIKKKDFGYF KKLKIKRNSF EIEIEKIEQL NYQDCPEISD
960 970 980 990 1000
FTTESDYHSM ISNGFVSHNC GLVKNFALMS HVTTDDSEGP LLRLAYNLGV
1010 1020 1030 1040 1050
QDILLVTGEE LNSRNAYLVL LNGQIIGIHN SPDYFVTTLR KMRRAGRIRE
1060 1070 1080 1090 1100
FVSICKNKAQ QTISVACDGG RLCRPVIIVD DQRPRLTQEH IEDLKDGLRT
1110 1120 1130 1140 1150
FDDFIREGII EYLDVNEEND SFLAWREAAI QPWTTHLEIE PFTMLGCVAG
1160 1170 1180 1190 1200
LIPYPHHNQS PRNTYQCAMG KQAIGAIAYN QLTRIDTLLY LLVHTQRPLC
1210 1220 1230 1240 1250
QTRTIDLLNW YKLPAGHNAT VAVMSYSGYD IEDALVMNKA SLDRGFGRCI
1260 1270 1280 1290 1300
VLKKQVTSIK KHGNDTSDRI FPPTPNDLRQ PKYGLLDSDG IAKPGELAQK
1310 1320 1330 1340 1350
GQILVNKYSP LNTIDATPNP ELIPDSAYKS SYMGYKYDNP AFIDKVLLTS
1360 1370 1380 1390 1400
GDDEQLLIKM LMRSTRRPEL GDKFSSRHGQ KGVCGIIVKQ EDMPFSDLGI
1410 1420 1430 1440 1450
CPDIIMNPHG FPSRMTIGKM IELLAGKAGV LSGKFGFGTC FGGDRVENIS
1460 1470 1480 1490 1500
KVLISKGFSY GGKDYVTSGV TGEPLACFIF FGPIFYQKLK HMVMDKMHAR
1510 1520 1530 1540 1550
ARGPTVTLTR QPTEGRARGG GLRLGEMERD CLIGYGASAL IMERLMISSD
1560 1570 1580 1590 1600
RFTVYACKNC GFLGYEGYCQ YCKSSVDIST IQIPYACKLL FQELQAMNIV

PRLKLVDS
Length:1,608
Mass (Da):183,030
Last modified:May 24, 2005 - v1
Checksum:i16D3C2AC009EAF5A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000111 Genomic DNA Translation: EAL63250.1
RefSeqiXP_636731.1, XM_631639.1

Genome annotation databases

EnsemblProtistsiEAL63250; EAL63250; DDB_G0288449
GeneIDi8626608
KEGGiddi:DDB_G0288449

Similar proteinsi

Entry informationi

Entry nameiRPC2_DICDI
AccessioniPrimary (citable) accession number: Q54IZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health