Reviewed, UniProtKB/Swiss-Prot Q54IL7 (P5CR1_DICDI)

Names and origin

Protein names

Recommended name:
    Pyrroline-5-carboxylate reductase 1
      Short name=P5C reductase 1
      Short name=P5CR 1
    EC=1.5.1.2

Gene names

Name:	pycr1
ORF Names:	DDB_G0288671

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-proline from L-glutamate 5-semialdehyde: step 1/1.
Subunit structure	Homodecamer; composed of 5 homodimers By similarity.
Sequence similarities	Belongs to the pyrroline-5-carboxylate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro pyrroline-5-carboxylate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Pyrroline-5-carboxylate reductase 1		PRO_0000328304
Regions
	Compositional bias	297 – 388	92	His-rich
	Compositional bias	319 – 326	8	Poly-Gln
	Compositional bias	448 – 453	6	Poly-Gln
	Compositional bias	456 – 474	19	Poly-Gln
	Compositional bias	487 – 496	10	Poly-Gln
	Compositional bias	504 – 532	29	Asn-rich

Sequences

	Sequence		Length	Mass (Da)
	Q54IL7-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 6D15ED4680D6244D	FASTA	551	63,009
	10 20 30 40 50 60 MKLYDNGVAV LGCGNLGNAI AKGLVASKQF KSNQIVLTKR NLSTIEPLKR EGYHVTTSNH 70 80 90 100 110 120 DAVSRCKIVI VCVVPAQLDD LLDSIKQSVT ENHIIISVVS GASIEDIRSH LEKDVPIVRA 130 140 150 160 170 180 MPNTAIQHCQ SMTCLAIRSS HQKSTNSPSD KAKDNALEVA KKIFNCLGMS IVLSEEQIVP 190 200 210 220 230 240 ATALCACGIA FFCRAIRAAA QGGCEIGFHA EDAIRIAAQT AKGAATLLLE NNFHPEYEID 250 260 270 280 290 300 KVTTPQGCTI AGLNQMEHAG FSSAMIKGIV TSSDKAASLY TQKQQNKKQQ QLKQQQHQQH 310 320 330 340 350 360 QHQQHQQHQQ QVQQQEPHQY QQQQQQSHQQ SQYNQGHNYG HQNQHHHNHD DQHQNYYNQD 370 380 390 400 410 420 QKRRNNRKHR SNENYDNHHH HNQQYQQHQQ PTQQESQEQT QQPEQTQSTN QSNQRRNSES 430 440 450 460 470 480 RNGKSPQKQP QKQSQVQQPS STTENTDQQQ QQQPPQEQQQ QQEQPQQPQE QQQQPNVVDE 490 500 510 520 530 540 AERPKEQQQQ PQQQQQTIDK KGYNNNRRGG RHYSYNNNYN SHHHRHNGIN KNSSSMYHDE 550 KRHEVKTEQI N

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000119 Genomic DNA. Translation: EAL63112.1.
RefSeq	XP_636614.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3388184.
KEGG	ddi:DDB_0232206.
Organism-specific databases
dictyBase	DDB_G0288671. pycr1.
Enzyme and pathway databases
BRENDA	1.5.1.2. 424.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR004455. NADP_OxRdtase_F420. IPR000304. P5CR. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11645. P5CR. 1 hit.
Pfam	PF03807. F420_oxidored. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P5CR1_DICDI

Accession

Primary (citable) accession number: Q54IL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)