Q54IL5 (UGT52_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-sugar-dependent glycosyltransferase 52
EC=2.4.1.173

Alternative name(s):
Sterol 3-beta-glucosyltransferase
UDP-glycosyltransferase 52

Gene names

Name:	ugt52
ORF Names:	DDB_G0288655

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1697 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the biosynthesis of sterol glucoside. Can use different sterols such as cholesterol, sitosterol, and ergosterol as sugar acceptors. Ref.2
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 1 FYVE-type zinc finger. Contains 1 GRAM domain. Contains 1 PH domain.
Sequence caution	The sequence AAD28546.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	lipid glycosylation Inferred from electronic annotation. Source: InterPro sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-sitosterol UDP-glucosyltransferase activity Inferred from direct assay Ref.2. Source: dictyBase carbohydrate binding Inferred from electronic annotation. Source: InterPro cholesterol UDP-glucosyltransferase activity Inferred from direct assay Ref.2. Source: dictyBase ergosterol UDP-glucosyltransferase activity Inferred from direct assay Ref.2. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW stigmasterol UDP-glucosyltransferase activity Inferred from direct assay Ref.2. Source: dictyBase tomatidine UDP-glucosyltransferase activity Inferred from direct assay Ref.2. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1697	1697	UDP-sugar-dependent glycosyltransferase 52		PRO_0000367478
Regions
	Domain	234 – 332	99	PH
	Domain	658 – 793	136	GRAM 1
	Domain	881 – 948	68	GRAM 2
	Zinc finger	1622 – 1685	64	FYVE-type
	Compositional bias	61 – 71	11	Poly-Asn
	Compositional bias	80 – 104	25	Gln-rich
	Compositional bias	150 – 159	10	Poly-Thr
	Compositional bias	374 – 377	4	Poly-Ser
	Compositional bias	587 – 595	9	Poly-Gln
	Compositional bias	604 – 607	4	Poly-Asn
	Compositional bias	622 – 627	6	Poly-Ser
	Compositional bias	630 – 633	4	Poly-Pro
	Compositional bias	711 – 722	12	Poly-Gln
	Compositional bias	840 – 851	12	Poly-Ser
	Compositional bias	858 – 861	4	Poly-Asn
	Compositional bias	992 – 1034	43	Ser-rich
	Compositional bias	1065 – 1080	16	Poly-Asn
	Compositional bias	1112 – 1118	7	Poly-Gln
	Compositional bias	1121 – 1131	11	Poly-Thr
	Compositional bias	1468 – 1479	12	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q54IL5 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 9364E429FD85A618	FASTA	1,697	190,915
	10 20 30 40 50 60 MDEFTSDSLT TSRLLRQISH SDSLSSVGSS SNRSNSNYEN KINNGIINDF DSTNANDGIS 70 80 90 100 110 120 NNSNNSNNNN NSSSILKIHQ SPQSILSTEQ QHQIQQYQQY HQQQIYESGY VPPHVYIFDP 130 140 150 160 170 180 DYKSNNDEED KEKRSLIELN KSTDLSNIKT TTTTTTTTTP PPLMIPENEE ENKQLRQEIL 190 200 210 220 230 240 NELSKSESFT KIKEPGEIYG FCECSLLYNH LTCANLVITS NYLIFLPQQQ VDSDYVLENY 250 260 270 280 290 300 LYKKGDFFWK KYWFVLTPDS ISWYKSSILK ETHYPNGTIL LSKIVTVDKL DPEETGKPFC 310 320 330 340 350 360 LQIMTNTSIH LIQLDTEPQL EHWYHEINRM QKNLNVTIPI GDIVSITMES NAALFFDSLC 370 380 390 400 410 420 LTLKKGENCL VTPSSSSNEV YSLLIKLWNR NRGVTVVEPE KQQRLLEFQK TFKLSQGVNL 430 440 450 460 470 480 LIETPCVFYQ DEEFSYEGVL YATTEGTLYF SSLDSVLIIP ESDIYAITID RKSDNRDALK 490 500 510 520 530 540 VYTTDYDVLF FDSIDDIETF FNVISTSLAR SNPKIFFSTV GQIPPIDEPK HDEKEHKSTY 550 560 570 580 590 600 QTISSGIRSW KIPIPSLPNF SIPIPKSIPI PLFRSKSSSH LDPQNSQQQQ QQQQQSSSLQ 610 620 630 640 650 660 LDDNNNNIVI PHTSNPTTII PSTSSSSAIP PPPSSTSSTS STTHEKTIII QKNSSINSTF 670 680 690 700 710 720 HDIFPLLPLD ETVIMFQNCS LYYYSYDSNV EGIVYITKSY IAFNPSPLDK QQQQQQQQQQ 730 740 750 760 770 780 QQPNSSSITS TTYDRDLDTD SDTDSESDFD YPKQSQHDNS VVMVNNNQYK VKEVLMKKAL 790 800 810 820 830 840 IPIEDIVSVT KERFLLFFNQ CVKIITLDHK WIFGSLNNIN SFYNLILETW KQIPKTLLDS 850 860 870 880 890 900 SSSSSSSSSS SLLSSSPNNN NSDLNINGNS EGSIFTPLES IKIKNKLGLP ADEVLITWFN 910 920 930 940 950 960 CTNFKGAQLK YGFLYISNNN ICFRSKFGFQ KRTIVIPLSQ VIEIKKYSAF IPNGIKITTA 970 980 990 1000 1010 1020 SHHEFQFASF IHRNRVYQIL YETWLKANNK KSNSSNSLSS SPTITSPLAI SPSIASPSIT 1030 1040 1050 1060 1070 1080 PPSSTPPSST TPSSTTPTIT SPTIHSTLPS TVVYNDIQEI IDGENNSNNN NNNNNTNNTN 1090 1100 1110 1120 1130 1140 KSNSFIGNVE EDVDKIKRSI KSLPILKIHS AQQQQQQQPK TTTTTTSTTT TNLISPRLLT 1150 1160 1170 1180 1190 1200 PLTITQSPGF SSLVNSPILP VKPLRITILT IGSRGDIQPF IALSLGLKEY GHNVTLATHE 1210 1220 1230 1240 1250 1260 LYRDLISKEF GLNYQPLGGD PRELMDLCVR NGIFTPKFIK EALSRFRSFI DDLLLTCWKA 1270 1280 1290 1300 1310 1320 VQNSNTQVLI ATPGCFAGPH IGEVLQIPFF NAFTMPFTRT RTYPNPFAPF ASHQMGGVFN 1330 1340 1350 1360 1370 1380 LATHVMMEKV LWQPISGQIN QWRTETLKIP PWNSSVSINE TYRMPYLYCF SKYLVPKPPD 1390 1400 1410 1420 1430 1440 WSGEIAITGY WTLKNQANSD SPPDDLIQFL NEESSTENDD IPIYIGFGSI VIDNPTALSL 1450 1460 1470 1480 1490 1500 LLIEAIKLSG KRAIISQGWG GLSIDEHNNN NNNNNNNNNG ENSDSNKSSL QSNRIYLLKK 1510 1520 1530 1540 1550 1560 PVDHSWLFEK VSLVISHGGA GTVAASLLAA KPTIVVPFFG DQFFWGERIK QTGIGTSIPF 1570 1580 1590 1600 1610 1620 DILTAKSLSS HIISILNEPS VRAKVNKMSH LLKREDGVKT AIDFIHRYLP FSFIPPREIP 1630 1640 1650 1660 1670 1680 FSSAPNSCMG CKQPFTLLHV MKARVHCHCC GKIFCESCTS HKCPIKKYRI NTPVRVCDKC 1690 FNDLQSNPSS NSFILND

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Cloning and functional expression of UGT genes encoding sterol glucosyltransferases from Saccharomyces cerevisiae, Candida albicans, Pichia pastoris, and Dictyostelium discoideum." Warnecke D.C., Erdmann R., Fahl A., Hube B., Mueller F., Zank T., Zaehringer U., Heinz E. J. Biol. Chem. 274:13048-13059(1999) [PubMed: 10224056] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 594-1697, FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000119 Genomic DNA. Translation: EAL63104.1. AF098916 mRNA. Translation: AAD28546.1. Different initiation.
RefSeq	XP_636616.1. XM_631524.1.
3D structure databases
HSSP	HSSP built from PDB template 1VFY based on UniProtKB P40343.
ProteinModelPortal	Q54IL5.
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191537; DDB0191537; DDB_G0288655.
GeneID	8626744.
GenomeReviews	Gene locus ugt52 in contig CM000154_GR.
KEGG	ddi:DDB_G0288655.
Organism-specific databases
dictyBase	DDB_G0288655. ugt52.
Phylogenomic databases
eggNOG	KOG1192.
GeneTree	EPrGT00050000002312.
InParanoid	Q54IL5.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR007235. Glyco_trans_28_C. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002213. UDP_glucos_trans. IPR000306. Znf_FYVE. IPR017455. Znf_FYVE-rel. IPR011011. Znf_FYVE_PHD. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:3.30.40.10. Znf_RING/FYVE/PHD. 1 hit.
KO	K05841.
PANTHER	PTHR11926. UDP_glucos_trans. 1 hit.
Pfam	PF01363. FYVE. 1 hit. PF04101. Glyco_tran_28_C. 1 hit. PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 1 hit. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00064. FYVE. 1 hit. SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50178. ZF_FYVE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UGT52_DICDI

Accession

Primary (citable) accession number: Q54IL5
Secondary accession number(s): Q9XYD4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)