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Q54IJ3

- GADB_DICDI

UniProt

Q54IJ3 - GADB_DICDI

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Protein

Glutamate decarboxylase B

Gene

gadB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Generates GABA from glutamate. Likely to be responsible for the metabolism of glutamate via the GABA shunt to succinate during growth.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Pyridoxal phosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601SubstrateBy similarity
Binding sitei81 – 811SubstrateBy similarity
Binding sitei208 – 2081Pyridoxal phosphateBy similarity
Binding sitei271 – 2711Pyridoxal phosphateBy similarity

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: dictyBase
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. aggregation involved in sorocarp development Source: dictyBase
  2. glutamate catabolic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase B (EC:4.1.1.15)
Gene namesi
Name:gadB
ORF Names:DDB_G0288715
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288715. gadB.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
  2. cytosol Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463Glutamate decarboxylase BPRO_0000391330Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei272 – 2721N6-(pyridoxal phosphate)lysineBy similarity

Expressioni

Developmental stagei

Expressed in growing cells, its mRNA continuously decreases during development. Highly expressed during phagocytosis of non-pathogenic bacteria.2 Publications

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0231439.

Structurei

3D structure databases

ProteinModelPortaliQ54IJ3.
SMRiQ54IJ3. Positions 22-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 1232Pyridoxal phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiCOG0076.
InParanoidiQ54IJ3.
KOiK01580.
OMAiNAPKEMS.
PhylomeDBiQ54IJ3.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
InterProiIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54IJ3 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLHIVDKHK SLYDNYVQSA TTEEIAKFKL ADDPNEPETI KKLIMDELLL
60 70 80 90 100
DGNSKQNLAT FCQTDLDKNI HSIMDKCIDK NMIDKDEYPQ SAEIENRCLH
110 120 130 140 150
ILADLWNAPD SSDTIGCSTT GSSEAAMLGG LALKWNWREN RKKLGLPYDK
160 170 180 190 200
PNIVTGPVQI CWHKFALYFD IEIREIPMEN GRYVMNSEEV LKRVDENTIG
210 220 230 240 250
VIPTLGVTFT LQYEDVFSIS NALDKFEKES GINIPIHVDA ASGGFVAPFI
260 270 280 290 300
QQEIIWDFRL PRVKSINASG HKFGLSPLGV GWVVWREKKD LHKDLVFNVN
310 320 330 340 350
YLGGNMSTFS LNFSRPGGQI IAQYYNFLRH GRNGYTLIQD ACAQQGIFIG
360 370 380 390 400
KELKKMGIFD LIYDGTGALP GVCWTISKNL PSEPLFNLYD LSEKLRSRGW
410 420 430 440 450
QVASYSLPSS MNDVVVSRVV IRHGFSRDLS SLFVKDVQNA IEYFQKHPIL
460
RSLEKDEGEN FHH
Length:463
Mass (Da):52,506
Last modified:May 24, 2005 - v1
Checksum:i8803CBD7A216B4E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000120 Genomic DNA. Translation: EAL63089.1.
RefSeqiXP_636593.1. XM_631501.1.

Genome annotation databases

EnsemblProtistsiDDB0231439; DDB0231439; DDB_G0288715.
GeneIDi8626767.
KEGGiddi:DDB_G0288715.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000120 Genomic DNA. Translation: EAL63089.1 .
RefSeqi XP_636593.1. XM_631501.1.

3D structure databases

ProteinModelPortali Q54IJ3.
SMRi Q54IJ3. Positions 22-448.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231439.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231439 ; DDB0231439 ; DDB_G0288715 .
GeneIDi 8626767.
KEGGi ddi:DDB_G0288715.

Organism-specific databases

dictyBasei DDB_G0288715. gadB.

Phylogenomic databases

eggNOGi COG0076.
InParanoidi Q54IJ3.
KOi K01580.
OMAi NAPKEMS.
PhylomeDBi Q54IJ3.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
InterProi IPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view ]
PANTHERi PTHR11999:SF1. PTHR11999:SF1. 1 hit.
Pfami PF00282. Pyridoxal_deC. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
TIGRFAMsi TIGR01788. Glu-decarb-GAD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
    Iranfar N., Fuller D., Loomis W.F.
    Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  3. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
    Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
    BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiGADB_DICDI
AccessioniPrimary (citable) accession number: Q54IJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: May 24, 2005
Last modified: October 1, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3