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Q54IJ3 (GADB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamate decarboxylase B
EC=4.1.1.15
Gene names
Name:gadB
ORF Names:DDB_G0288715
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length463 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Generates GABA from glutamate. Likely to be responsible for the metabolism of glutamate via the GABA shunt to succinate during growth.

Catalytic activity

L-glutamate = 4-aminobutanoate + CO2.

Cofactor

Pyridoxal phosphate By similarity.

Subunit structure

Homohexamer By similarity.

Developmental stage

Expressed in growing cells, its mRNA continuously decreases during development. Highly expressed during phagocytosis of non-pathogenic bacteria. Ref.2 Ref.3

Sequence similarities

Belongs to the group II decarboxylase family.

Ontologies

Keywords
   LigandPyridoxal phosphate
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamate catabolic process

Inferred from sequence or structural similarity. Source: dictyBase

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: dictyBase

   Molecular_functionglutamate decarboxylase activity

Inferred from sequence or structural similarity. Source: dictyBase

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 463463Glutamate decarboxylase B
PRO_0000391330

Regions

Region122 – 1232Pyridoxal phosphate binding By similarity

Sites

Binding site601Substrate By similarity
Binding site811Substrate By similarity
Binding site2081Pyridoxal phosphate By similarity
Binding site2711Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue2721N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54IJ3 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 8803CBD7A216B4E3

FASTA46352,506
        10         20         30         40         50         60 
MPLHIVDKHK SLYDNYVQSA TTEEIAKFKL ADDPNEPETI KKLIMDELLL DGNSKQNLAT 

        70         80         90        100        110        120 
FCQTDLDKNI HSIMDKCIDK NMIDKDEYPQ SAEIENRCLH ILADLWNAPD SSDTIGCSTT 

       130        140        150        160        170        180 
GSSEAAMLGG LALKWNWREN RKKLGLPYDK PNIVTGPVQI CWHKFALYFD IEIREIPMEN 

       190        200        210        220        230        240 
GRYVMNSEEV LKRVDENTIG VIPTLGVTFT LQYEDVFSIS NALDKFEKES GINIPIHVDA 

       250        260        270        280        290        300 
ASGGFVAPFI QQEIIWDFRL PRVKSINASG HKFGLSPLGV GWVVWREKKD LHKDLVFNVN 

       310        320        330        340        350        360 
YLGGNMSTFS LNFSRPGGQI IAQYYNFLRH GRNGYTLIQD ACAQQGIFIG KELKKMGIFD 

       370        380        390        400        410        420 
LIYDGTGALP GVCWTISKNL PSEPLFNLYD LSEKLRSRGW QVASYSLPSS MNDVVVSRVV 

       430        440        450        460 
IRHGFSRDLS SLFVKDVQNA IEYFQKHPIL RSLEKDEGEN FHH

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
Iranfar N., Fuller D., Loomis W.F.
Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[3]"Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAFI02000120 Genomic DNA. Translation: EAL63089.1.
RefSeqXP_636593.1. XM_631501.1.

3D structure databases

ProteinModelPortalQ54IJ3.
SMRQ54IJ3. Positions 22-448.
ModBaseSearch...

Protein-protein interaction databases

STRING44689.DDB_0231439.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231439; DDB0231439; DDB_G0288715.
GeneID8626767.
KEGGddi:DDB_G0288715.

Organism-specific databases

dictyBaseDDB_G0288715. gadB.

Phylogenomic databases

eggNOGCOG0076.
InParanoidQ54IJ3.
KOK01580.
OMAMENCIEN.
ProtClustDBCLSZ2728831.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
InterProIPR010107. Glutamate_decarboxylase.
IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
[Graphical view]
PANTHERPTHR11999:SF1. PTHR11999:SF1. 1 hit.
PfamPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMsTIGR01788. Glu-decarb-GAD. 1 hit.
PROSITEPS00392. DDC_GAD_HDC_YDC. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGADB_DICDI
AccessionPrimary (citable) accession number: Q54IJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: May 24, 2005
Last modified: May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

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