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Q54IJ3

- GADB_DICDI

UniProt

Q54IJ3 - GADB_DICDI

Protein

Glutamate decarboxylase B

Gene

gadB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Generates GABA from glutamate. Likely to be responsible for the metabolism of glutamate via the GABA shunt to succinate during growth.

    Catalytic activityi

    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei60 – 601SubstrateBy similarity
    Binding sitei81 – 811SubstrateBy similarity
    Binding sitei208 – 2081Pyridoxal phosphateBy similarity
    Binding sitei271 – 2711Pyridoxal phosphateBy similarity

    GO - Molecular functioni

    1. glutamate decarboxylase activity Source: dictyBase
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. aggregation involved in sorocarp development Source: dictyBase
    2. glutamate catabolic process Source: dictyBase

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Pyridoxal phosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase B (EC:4.1.1.15)
    Gene namesi
    Name:gadB
    ORF Names:DDB_G0288715
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0288715. gadB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase
    2. cytosol Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 463463Glutamate decarboxylase BPRO_0000391330Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei272 – 2721N6-(pyridoxal phosphate)lysineBy similarity

    Expressioni

    Developmental stagei

    Expressed in growing cells, its mRNA continuously decreases during development. Highly expressed during phagocytosis of non-pathogenic bacteria.2 Publications

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0231439.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54IJ3.
    SMRiQ54IJ3. Positions 22-448.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni122 – 1232Pyridoxal phosphate bindingBy similarity

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0076.
    InParanoidiQ54IJ3.
    KOiK01580.
    OMAiNAPKEMS.
    PhylomeDBiQ54IJ3.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR010107. Glutamate_decarboxylase.
    IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11999:SF1. PTHR11999:SF1. 1 hit.
    PfamiPF00282. Pyridoxal_deC. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    TIGRFAMsiTIGR01788. Glu-decarb-GAD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q54IJ3-1 [UniParc]FASTAAdd to Basket

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    MPLHIVDKHK SLYDNYVQSA TTEEIAKFKL ADDPNEPETI KKLIMDELLL    50
    DGNSKQNLAT FCQTDLDKNI HSIMDKCIDK NMIDKDEYPQ SAEIENRCLH 100
    ILADLWNAPD SSDTIGCSTT GSSEAAMLGG LALKWNWREN RKKLGLPYDK 150
    PNIVTGPVQI CWHKFALYFD IEIREIPMEN GRYVMNSEEV LKRVDENTIG 200
    VIPTLGVTFT LQYEDVFSIS NALDKFEKES GINIPIHVDA ASGGFVAPFI 250
    QQEIIWDFRL PRVKSINASG HKFGLSPLGV GWVVWREKKD LHKDLVFNVN 300
    YLGGNMSTFS LNFSRPGGQI IAQYYNFLRH GRNGYTLIQD ACAQQGIFIG 350
    KELKKMGIFD LIYDGTGALP GVCWTISKNL PSEPLFNLYD LSEKLRSRGW 400
    QVASYSLPSS MNDVVVSRVV IRHGFSRDLS SLFVKDVQNA IEYFQKHPIL 450
    RSLEKDEGEN FHH 463
    Length:463
    Mass (Da):52,506
    Last modified:May 24, 2005 - v1
    Checksum:i8803CBD7A216B4E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000120 Genomic DNA. Translation: EAL63089.1.
    RefSeqiXP_636593.1. XM_631501.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231439; DDB0231439; DDB_G0288715.
    GeneIDi8626767.
    KEGGiddi:DDB_G0288715.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000120 Genomic DNA. Translation: EAL63089.1 .
    RefSeqi XP_636593.1. XM_631501.1.

    3D structure databases

    ProteinModelPortali Q54IJ3.
    SMRi Q54IJ3. Positions 22-448.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231439.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231439 ; DDB0231439 ; DDB_G0288715 .
    GeneIDi 8626767.
    KEGGi ddi:DDB_G0288715.

    Organism-specific databases

    dictyBasei DDB_G0288715. gadB.

    Phylogenomic databases

    eggNOGi COG0076.
    InParanoidi Q54IJ3.
    KOi K01580.
    OMAi NAPKEMS.
    PhylomeDBi Q54IJ3.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    InterProi IPR010107. Glutamate_decarboxylase.
    IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view ]
    PANTHERi PTHR11999:SF1. PTHR11999:SF1. 1 hit.
    Pfami PF00282. Pyridoxal_deC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    TIGRFAMsi TIGR01788. Glu-decarb-GAD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Genome-wide expression analyses of gene regulation during early development of Dictyostelium discoideum."
      Iranfar N., Fuller D., Loomis W.F.
      Eukaryot. Cell 2:664-670(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    3. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
      Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
      BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiGADB_DICDI
    AccessioniPrimary (citable) accession number: Q54IJ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 9, 2010
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3