Q54IJ0 (PRPD_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable 2-methylcitrate dehydratase
EC=4.2.1.79

Gene names

Name:	prpD
ORF Names:	DDB_G0288681

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the dehydration of 2-methylcitrate to 2-methyl-cis-aconitate By similarity.
Catalytic activity	(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O.
Cofactor	Binds 1 2Fe-2S cluster By similarity.
Pathway	Organic acid metabolism; propanoate degradation.
Sequence similarities	Belongs to the PrpD family.

Ontologies

Keywords
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	propionate catabolic process, 2-methylcitrate cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 2-methylcitrate dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Probable 2-methylcitrate dehydratase		PRO_0000328266

Sequences

	Sequence		Length	Mass (Da)
	Q54IJ0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: CE1B4F05C0C9821A	FASTA	492	54,984
	10 20 30 40 50 60 MNNNIEQKWD KVLEDIVDYV IGFDVEGNSK EAMETAFLAL FDTIGCGFLG LKSTGCTKLM 70 80 90 100 110 120 GPTIPDTRIP GGCRVPGTDY QLNPIEAAFN IGCMNRWLDF NDCWLANEWG HPSDNISAII 130 140 150 160 170 180 AAAEYISNDN ISKSKKPLLM KDILCAMVKC YEIQGVLALD NSFNKIGLDH VILVKIASTA 190 200 210 220 230 240 VTCHLLGGSR IQILNALSNA FIDGHPLRTY RHSPNTGSRK GWAAGDAASR AVQLSLMAMA 250 260 270 280 290 300 GEMGYPTCLS APKWGFYHVY MSDKPFQFNQ RSNYSTYVME NILFKVLYPA EFHGQTAIEC 310 320 330 340 350 360 AIQLHPIFQD KGGLNSVESI TIETTEPAIR IIDKRGILRN PSDRDHCLQF IVSVALIFGE 370 380 390 400 410 420 LKSSHYEDQV AIKDTRIDLL REKITCIENL QFSLDYLNPD KRSIANSITI LFKDGETHKH 430 440 450 460 470 480 QIDYPIGHRN RRSEAIPLLQ QKFLNSLNST LFTSSSIDQI KNLYENPSKF NNLSILDFMS 490 IFSLSIASTP IS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Iranfar N., Loomis W.F. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 163-492. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000120 Genomic DNA. Translation: EAL63072.1. U83088 mRNA. Translation: AAB40931.1.
RefSeq	XP_636596.1. XM_631504.1.
3D structure databases
ProteinModelPortal	Q54IJ0.
SMR	Q54IJ0. Positions 8-483.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0191366.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0191366; DDB0191366; DDB_G0288681.
GeneID	8626771.
KEGG	ddi:DDB_G0288681.
Organism-specific databases
dictyBase	DDB_G0288681. prpD.
Phylogenomic databases
eggNOG	COG2079.
KO	K01720.
OMA	PNAGSRK.
Enzyme and pathway databases
UniPathway	UPA00946.
Family and domain databases
InterPro	IPR012705. 2Me_IsoCit_deHydtase_PrpD. IPR005656. MmgE_PrpD. [Graphical view]
PANTHER	PTHR16943. PTHR16943. 1 hit.
Pfam	PF03972. MmgE_PrpD. 1 hit. [Graphical view]
SUPFAM	SSF103378. MmgE_PrpD. 1 hit.
TIGRFAMs	TIGR02330. prpD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRPD_DICDI

Accession

Primary (citable) accession number: Q54IJ0
Secondary accession number(s): P90535

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 29, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)