ID   SMT1_DICDI              Reviewed;         354 AA.
AC   Q54I98;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   27-MAR-2024, entry version 113.
DE   RecName: Full=Probable cycloartenol-C-24-methyltransferase 1;
DE            EC=2.1.1.41;
DE   AltName: Full=24-sterol C-methyltransferase 1;
DE   AltName: Full=Sterol 24-C-methyltransferase 1;
GN   Name=smt1; ORFNames=DDB_G0288907;
OS   Dictyostelium discoideum (Social amoeba).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=AX2;
RX   PubMed=16926386; DOI=10.1074/mcp.m600113-mcp200;
RA   Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M.,
RA   Soldati T.;
RT   "Proteomics fingerprinting of phagosome maturation and evidence for the
RT   role of a Galpha during uptake.";
RL   Mol. Cell. Proteomics 5:2228-2243(2006).
CC   -!- FUNCTION: Catalyzes the methyl transfer from S-adenosyl-methionine to
CC       the C-24 of cycloartenol to form 24-methylene cycloartenol.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + zymosterol = fecosterol + H(+) + S-
CC         adenosyl-L-homocysteine; Xref=Rhea:RHEA:21128, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17038, ChEBI:CHEBI:18252, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789; EC=2.1.1.41;
CC   -!- PATHWAY: Steroid biosynthesis; sterol biosynthesis.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. Erg6/SMT family. {ECO:0000255|PROSITE-ProRule:PRU01022}.
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DR   EMBL; AAFI02000126; EAL62977.1; -; Genomic_DNA.
DR   RefSeq; XP_636481.1; XM_631389.1.
DR   AlphaFoldDB; Q54I98; -.
DR   SMR; Q54I98; -.
DR   STRING; 44689.Q54I98; -.
DR   PaxDb; 44689-DDB0237965; -.
DR   EnsemblProtists; EAL62977; EAL62977; DDB_G0288907.
DR   GeneID; 8626864; -.
DR   KEGG; ddi:DDB_G0288907; -.
DR   dictyBase; DDB_G0288907; smtA.
DR   eggNOG; KOG1269; Eukaryota.
DR   HOGENOM; CLU_039068_5_0_1; -.
DR   InParanoid; Q54I98; -.
DR   OMA; AFNKAMH; -.
DR   PhylomeDB; Q54I98; -.
DR   UniPathway; UPA00766; -.
DR   PRO; PR:Q54I98; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005783; C:endoplasmic reticulum; IDA:dictyBase.
DR   GO; GO:0005811; C:lipid droplet; IDA:dictyBase.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   GO; GO:0003838; F:sterol 24-C-methyltransferase activity; ISS:dictyBase.
DR   GO; GO:0006972; P:hyperosmotic response; IEP:dictyBase.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0009617; P:response to bacterium; HEP:dictyBase.
DR   GO; GO:0016126; P:sterol biosynthetic process; IBA:GO_Central.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR013216; Methyltransf_11.
DR   InterPro; IPR030384; MeTrfase_SMT.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR013705; Sterol_MeTrfase_C.
DR   PANTHER; PTHR44068:SF1; HYPOTHETICAL LOC100005854; 1.
DR   PANTHER; PTHR44068; ZGC:194242; 1.
DR   Pfam; PF08241; Methyltransf_11; 1.
DR   Pfam; PF08498; Sterol_MT_C; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51685; SAM_MT_ERG6_SMT; 1.
PE   1: Evidence at protein level;
KW   Lipid biosynthesis; Lipid metabolism; Methyltransferase;
KW   Reference proteome; S-adenosyl-L-methionine; Steroid biosynthesis;
KW   Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase.
FT   CHAIN           1..354
FT                   /note="Probable cycloartenol-C-24-methyltransferase 1"
FT                   /id="PRO_0000328281"
SQ   SEQUENCE   354 AA;  39559 MW;  E5E6C3B08A002B21 CRC64;
     MNTQQRAMEG DLSRIVAISR KEKDAVSRND KVDDTLNGYK ELFKGNDDKA IQARKNNYTH
     MVNTFYDLAT DFYEFGWGQS FHFATRHKYE SFEASIARHE MYMAHQLGLF PGMKVIDIGC
     GVGGPMRTIA RFSGANVVGL NNNEYQIQRG KRLNESAGLS HLCSFIKADF MHVPVEDNTY
     DCAYQIEATC HAPDLVGLYK EVFRIVKPGG LFGGYEWIMT NKFNPEDPVE VNIKKQIELG
     NGLPDLVKPA EIINAAKAAG FEVITAFDVA ETSELPWYLP LSSGVSITGF LHTGVGRYLT
     GKFTQLLEIV KLAPAGSYNT NVWLQNAATF LVQGGEKQIF SPMFFLLCRK PSTD
//