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Q54I98 (SMT1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cycloartenol-C-24-methyltransferase 1

EC=2.1.1.41
Alternative name(s):
24-sterol C-methyltransferase 1
Sterol 24-C-methyltransferase 1
Gene names
Name:smt1
ORF Names:DDB_G0288907
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol By similarity.

Catalytic activity

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathway

Steroid biosynthesis; sterol biosynthesis.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Probable cycloartenol-C-24-methyltransferase 1
PRO_0000328281

Sequences

Sequence LengthMass (Da)Tools
Q54I98 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: E5E6C3B08A002B21

FASTA35439,559
        10         20         30         40         50         60 
MNTQQRAMEG DLSRIVAISR KEKDAVSRND KVDDTLNGYK ELFKGNDDKA IQARKNNYTH 

        70         80         90        100        110        120 
MVNTFYDLAT DFYEFGWGQS FHFATRHKYE SFEASIARHE MYMAHQLGLF PGMKVIDIGC 

       130        140        150        160        170        180 
GVGGPMRTIA RFSGANVVGL NNNEYQIQRG KRLNESAGLS HLCSFIKADF MHVPVEDNTY 

       190        200        210        220        230        240 
DCAYQIEATC HAPDLVGLYK EVFRIVKPGG LFGGYEWIMT NKFNPEDPVE VNIKKQIELG 

       250        260        270        280        290        300 
NGLPDLVKPA EIINAAKAAG FEVITAFDVA ETSELPWYLP LSSGVSITGF LHTGVGRYLT 

       310        320        330        340        350 
GKFTQLLEIV KLAPAGSYNT NVWLQNAATF LVQGGEKQIF SPMFFLLCRK PSTD 

« Hide

References

[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000126 Genomic DNA. Translation: EAL62977.1.

3D structure databases

ProteinModelPortalQ54I98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0188166.

Proteomic databases

PRIDEQ54I98.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0237965; DDB0237965; DDB_G0288907.
KEGGddi:DDB_G0288907.

Organism-specific databases

dictyBaseDDB_G0288907. smtA.

Phylogenomic databases

eggNOGCOG0500.
KOK00559.
OMAFHFCRFS.
PhylomeDBQ54I98.
ProtClustDBCLSZ2429830.

Enzyme and pathway databases

UniPathwayUPA00766.

Family and domain databases

InterProIPR025810. ERG6.
IPR013216. Methyltransf_11.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
PROSITEPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSMT1_DICDI
AccessionPrimary (citable) accession number: Q54I98
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase