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Q54I98

- SMT1_DICDI

UniProt

Q54I98 - SMT1_DICDI

Protein

Probable cycloartenol-C-24-methyltransferase 1

Gene

smt1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

    Pathwayi

    GO - Molecular functioni

    1. sterol 24-C-methyltransferase activity Source: dictyBase

    GO - Biological processi

    1. hyperosmotic response Source: dictyBase
    2. response to bacterium Source: dictyBase
    3. sterol biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00766.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable cycloartenol-C-24-methyltransferase 1 (EC:2.1.1.41)
    Alternative name(s):
    24-sterol C-methyltransferase 1
    Sterol 24-C-methyltransferase 1
    Gene namesi
    Name:smt1
    ORF Names:DDB_G0288907
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0288907. smtA.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: dictyBase
    2. phagocytic vesicle Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Probable cycloartenol-C-24-methyltransferase 1PRO_0000328281Add
    BLAST

    Proteomic databases

    PRIDEiQ54I98.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0188166.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54I98.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0500.
    KOiK00559.
    OMAiFHFCRFS.
    PhylomeDBiQ54I98.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025810. ERG6.
    IPR013216. Methyltransf_11.
    IPR029063. SAM-dependent_MTases-like.
    IPR013705. Sterol_MeTrfase_C.
    [Graphical view]
    PfamiPF08241. Methyltransf_11. 1 hit.
    PF08498. Sterol_MT_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54I98-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNTQQRAMEG DLSRIVAISR KEKDAVSRND KVDDTLNGYK ELFKGNDDKA    50
    IQARKNNYTH MVNTFYDLAT DFYEFGWGQS FHFATRHKYE SFEASIARHE 100
    MYMAHQLGLF PGMKVIDIGC GVGGPMRTIA RFSGANVVGL NNNEYQIQRG 150
    KRLNESAGLS HLCSFIKADF MHVPVEDNTY DCAYQIEATC HAPDLVGLYK 200
    EVFRIVKPGG LFGGYEWIMT NKFNPEDPVE VNIKKQIELG NGLPDLVKPA 250
    EIINAAKAAG FEVITAFDVA ETSELPWYLP LSSGVSITGF LHTGVGRYLT 300
    GKFTQLLEIV KLAPAGSYNT NVWLQNAATF LVQGGEKQIF SPMFFLLCRK 350
    PSTD 354
    Length:354
    Mass (Da):39,559
    Last modified:May 24, 2005 - v1
    Checksum:iE5E6C3B08A002B21
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000126 Genomic DNA. Translation: EAL62977.1.
    RefSeqiXP_636481.1. XM_631389.1.

    Genome annotation databases

    EnsemblProtistsiDDB0237965; DDB0237965; DDB_G0288907.
    GeneIDi8626864.
    KEGGiddi:DDB_G0288907.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000126 Genomic DNA. Translation: EAL62977.1 .
    RefSeqi XP_636481.1. XM_631389.1.

    3D structure databases

    ProteinModelPortali Q54I98.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0188166.

    Proteomic databases

    PRIDEi Q54I98.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0237965 ; DDB0237965 ; DDB_G0288907 .
    GeneIDi 8626864.
    KEGGi ddi:DDB_G0288907.

    Organism-specific databases

    dictyBasei DDB_G0288907. smtA.

    Phylogenomic databases

    eggNOGi COG0500.
    KOi K00559.
    OMAi FHFCRFS.
    PhylomeDBi Q54I98.

    Enzyme and pathway databases

    UniPathwayi UPA00766 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR025810. ERG6.
    IPR013216. Methyltransf_11.
    IPR029063. SAM-dependent_MTases-like.
    IPR013705. Sterol_MeTrfase_C.
    [Graphical view ]
    Pfami PF08241. Methyltransf_11. 1 hit.
    PF08498. Sterol_MT_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51685. SAM_MT_ERG6_SMT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiSMT1_DICDI
    AccessioniPrimary (citable) accession number: Q54I98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3