Reviewed, UniProtKB/Swiss-Prot Q54I98 (SMT1_DICDI)

Names and origin

Protein names

Recommended name:
    Probable cycloartenol-C-24-methyltransferase 1
    EC=2.1.1.41
Alternative name(s):
    24-sterol C-methyltransferase 1
    Sterol 24-C-methyltransferase 1

Gene names

Name:	smt1
ORF Names:	DDB_G0288907

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol By similarity.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; sterol biosynthesis.
Sequence similarities	Belongs to the methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hyperosmotic response Inferred from expression pattern. Source: dictyBase sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	phagocytic vesicle Ref.2 Inferred from direct assay. Source: dictyBase
Molecular function	sterol 24-C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Probable cycloartenol-C-24-methyltransferase 1		PRO_0000328281

Sequences

	Sequence		Length	Mass (Da)
	Q54I98-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: E5E6C3B08A002B21	FASTA	354	39,559
	10 20 30 40 50 60 MNTQQRAMEG DLSRIVAISR KEKDAVSRND KVDDTLNGYK ELFKGNDDKA IQARKNNYTH 70 80 90 100 110 120 MVNTFYDLAT DFYEFGWGQS FHFATRHKYE SFEASIARHE MYMAHQLGLF PGMKVIDIGC 130 140 150 160 170 180 GVGGPMRTIA RFSGANVVGL NNNEYQIQRG KRLNESAGLS HLCSFIKADF MHVPVEDNTY 190 200 210 220 230 240 DCAYQIEATC HAPDLVGLYK EVFRIVKPGG LFGGYEWIMT NKFNPEDPVE VNIKKQIELG 250 260 270 280 290 300 NGLPDLVKPA EIINAAKAAG FEVITAFDVA ETSELPWYLP LSSGVSITGF LHTGVGRYLT 310 320 330 340 350 GKFTQLLEIV KLAPAGSYNT NVWLQNAATF LVQGGEKQIF SPMFFLLCRK PSTD

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000126 Genomic DNA. Translation: EAL62977.1.
RefSeq	XP_636481.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3388054.
KEGG	ddi:DDB_0237965.
Organism-specific databases
dictyBase	DDB_G0288907. smt1.
Phylogenomic databases
OMA	Q54I98. FVKGDFM.
Enzyme and pathway databases
BRENDA	2.1.1.41. 424.
Family and domain databases
InterPro	IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMT1_DICDI

Accession

Primary (citable) accession number: Q54I98

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 5, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)