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Protein

Probable cycloartenol-C-24-methyltransferase 1

Gene

smt1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol.By similarity

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathway:isterol biosynthesis

This protein is involved in the pathway sterol biosynthesis, which is part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the pathway sterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • ergosterol biosynthetic process Source: GO_Central
  • hyperosmotic response Source: dictyBase
  • methylation Source: GO_Central
  • response to bacterium Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00766.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cycloartenol-C-24-methyltransferase 1 (EC:2.1.1.41)
Alternative name(s):
24-sterol C-methyltransferase 1
Sterol 24-C-methyltransferase 1
Gene namesi
Name:smt1
ORF Names:DDB_G0288907
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288907. smtA.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: dictyBase
  • phagocytic vesicle Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 354354Probable cycloartenol-C-24-methyltransferase 1PRO_0000328281Add
BLAST

Proteomic databases

PRIDEiQ54I98.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0237965.

Structurei

3D structure databases

ProteinModelPortaliQ54I98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0500.
InParanoidiQ54I98.
KOiK00559.
OMAiFHFCRFS.
PhylomeDBiQ54I98.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54I98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTQQRAMEG DLSRIVAISR KEKDAVSRND KVDDTLNGYK ELFKGNDDKA
60 70 80 90 100
IQARKNNYTH MVNTFYDLAT DFYEFGWGQS FHFATRHKYE SFEASIARHE
110 120 130 140 150
MYMAHQLGLF PGMKVIDIGC GVGGPMRTIA RFSGANVVGL NNNEYQIQRG
160 170 180 190 200
KRLNESAGLS HLCSFIKADF MHVPVEDNTY DCAYQIEATC HAPDLVGLYK
210 220 230 240 250
EVFRIVKPGG LFGGYEWIMT NKFNPEDPVE VNIKKQIELG NGLPDLVKPA
260 270 280 290 300
EIINAAKAAG FEVITAFDVA ETSELPWYLP LSSGVSITGF LHTGVGRYLT
310 320 330 340 350
GKFTQLLEIV KLAPAGSYNT NVWLQNAATF LVQGGEKQIF SPMFFLLCRK

PSTD
Length:354
Mass (Da):39,559
Last modified:May 24, 2005 - v1
Checksum:iE5E6C3B08A002B21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000126 Genomic DNA. Translation: EAL62977.1.
RefSeqiXP_636481.1. XM_631389.1.

Genome annotation databases

EnsemblProtistsiDDB0237965; DDB0237965; DDB_G0288907.
GeneIDi8626864.
KEGGiddi:DDB_G0288907.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000126 Genomic DNA. Translation: EAL62977.1.
RefSeqiXP_636481.1. XM_631389.1.

3D structure databases

ProteinModelPortaliQ54I98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0237965.

Proteomic databases

PRIDEiQ54I98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0237965; DDB0237965; DDB_G0288907.
GeneIDi8626864.
KEGGiddi:DDB_G0288907.

Organism-specific databases

dictyBaseiDDB_G0288907. smtA.

Phylogenomic databases

eggNOGiCOG0500.
InParanoidiQ54I98.
KOiK00559.
OMAiFHFCRFS.
PhylomeDBiQ54I98.

Enzyme and pathway databases

UniPathwayiUPA00766.

Miscellaneous databases

PROiQ54I98.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR013216. Methyltransf_11.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
    Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
    Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: AX2.

Entry informationi

Entry nameiSMT1_DICDI
AccessioniPrimary (citable) accession number: Q54I98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: June 24, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.