Q54I98 (SMT1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cycloartenol-C-24-methyltransferase 1
EC=2.1.1.41

Alternative name(s):
24-sterol C-methyltransferase 1
Sterol 24-C-methyltransferase 1

Gene names

Name:	smt1
ORF Names:	DDB_G0288907

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of cycloartenol to form 24-methylene cycloartenol By similarity.
Catalytic activity	S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.
Pathway	Steroid biosynthesis; sterol biosynthesis.
Sequence similarities	Belongs to the class I-like SAM-binding methyltransferase superfamily. ERG6 family.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hyperosmotic response Inferred from expression pattern PubMed 17517120. Source: dictyBase response to bacterium Inferred from expression pattern PubMed 19482547. Source: dictyBase sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	endoplasmic reticulum Inferred from sequence or structural similarity. Source: dictyBase phagocytic vesicle Inferred from direct assay Ref.2PubMed 19482547. Source: dictyBase
Molecular_function	sterol 24-C-methyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Probable cycloartenol-C-24-methyltransferase 1		PRO_0000328281

Sequences

	Sequence		Length	Mass (Da)
	Q54I98 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: E5E6C3B08A002B21	FASTA	354	39,559
	10 20 30 40 50 60 MNTQQRAMEG DLSRIVAISR KEKDAVSRND KVDDTLNGYK ELFKGNDDKA IQARKNNYTH 70 80 90 100 110 120 MVNTFYDLAT DFYEFGWGQS FHFATRHKYE SFEASIARHE MYMAHQLGLF PGMKVIDIGC 130 140 150 160 170 180 GVGGPMRTIA RFSGANVVGL NNNEYQIQRG KRLNESAGLS HLCSFIKADF MHVPVEDNTY 190 200 210 220 230 240 DCAYQIEATC HAPDLVGLYK EVFRIVKPGG LFGGYEWIMT NKFNPEDPVE VNIKKQIELG 250 260 270 280 290 300 NGLPDLVKPA EIINAAKAAG FEVITAFDVA ETSELPWYLP LSSGVSITGF LHTGVGRYLT 310 320 330 340 350 GKFTQLLEIV KLAPAGSYNT NVWLQNAATF LVQGGEKQIF SPMFFLLCRK PSTD

References

[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000126 Genomic DNA. Translation: EAL62977.1.
RefSeq	XP_636481.1. XM_631389.1.
3D structure databases
ProteinModelPortal	Q54I98.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDBDRAFT_0188166.
Proteomic databases
PRIDE	Q54I98.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0237965; DDB0237965; DDB_G0288907.
GeneID	8626864.
KEGG	ddi:DDB_G0288907.
Organism-specific databases
dictyBase	DDB_G0288907. smt1.
Phylogenomic databases
eggNOG	COG0500.
KO	K00559.
OMA	KLFTPMM.
ProtClustDB	CLSZ2429830.
Enzyme and pathway databases
UniPathway	UPA00766.
Family and domain databases
InterPro	IPR025810. ERG6. IPR013216. Methyltransf_11. IPR013705. Sterol_MeTrfase_C. [Graphical view]
Pfam	PF08241. Methyltransf_11. 1 hit. PF08498. Sterol_MT_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMT1_DICDI

Accession

Primary (citable) accession number: Q54I98

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)