Reviewed, UniProtKB/Swiss-Prot Q54I90 (NDUV1_DICDI)

Names and origin

Protein names

Recommended name:
    NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial
    EC=1.6.5.3
    EC=1.6.99.3
Alternative name(s):
    NADH-ubiquinone oxidoreductase 51 kDa subunit
    Complex I-51kD
      Short name=CI-51kD

Gene names

Name:	ndufv1
Synonyms:	qinA, rcdG, veg107
ORF Names:	DDB_G0288875

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Subunit structure	Complex I is composed of about 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side By similarity.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial respiratory chain complex I Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 479		NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial		PRO_0000327999
Regions
	Nucleotide binding	103 – 112	10	NAD(H) By similarity
	Nucleotide binding	216 – 264	49	FMN By similarity
Sites
	Metal binding	396	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	399	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	402	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	442	1	Iron-sulfur (4Fe-4S) Potential
Experimental info
	Sequence conflict	24	1	D → N in AAB03672. Ref.1
	Sequence conflict	120	1	K → E in AAB03672. Ref.1
	Sequence conflict	231	1	E → G in AAB03672. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q54I90-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 1316E7409080C448	FASTA	479	52,691
	10 20 30 40 50 60 MMNLGNRVKS VIKLTSTTGL TGKDFQATKV ATFSTAQVQQ AQENVRSYGG LKDKDRIFTN 70 80 90 100 110 120 LYGEHDVYLK GAIARGDWYK TKNIIDKGKD WILKEMMASG LRGRGGAGFP SGLKWSFMPK 130 140 150 160 170 180 TTSKDRPQYL VINADEGEPG TCKDREIMRH DPHKLIEGCL LAGFAMRACA AYIYIRGEFH 190 200 210 220 230 240 YEAKVLEQAI DEAYKAGLIG ENACGTGYKF DVYVHRGAGA YICGEETALI ESIEGKQGKP 250 260 270 280 290 300 RLKPPFPAMA GLYGCPTTVT NVETVAVAPT ILRRGGAWFA SFGRPKNAGT KLFCISGHVN 310 320 330 340 350 360 NPCTVEEEMS IPLRELIDKH CGGVIGGWDN LKGVIPGGSS VPVLPKNICD NVLMDFDDLR 370 380 390 400 410 420 QHRSGLGTAA VIVMNKETDM IAAIARLSKF YKHESCGQCT PCREGVGWLY DITDRLVTGN 430 440 450 460 470 AKPDEIDSLE EISRQIEGHT ICALGDAAAW PVQGLIRHFR PEIEDRIKQF QLNKKQSPF

References

[1]

"Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome." Kuspa A., Loomis W.F. Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed: 8643615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: AX4.

[2]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U61989 mRNA. Translation: AAB03672.1. AAFI02000126 Genomic DNA. Translation: EAL62961.1.
RefSeq	XP_636489.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3388038.
KEGG	ddi:DDB_0191420.
Organism-specific databases
dictyBase	DDB_G0288875. ndufv1.
Phylogenomic databases
OMA	Q54I90. PCNVEEE.
Enzyme and pathway databases
BRENDA	1.6.5.3. 424. 1.6.99.3. 424.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUV1_DICDI

Accession

Primary (citable) accession number: Q54I90
Secondary accession number(s): Q23923

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)