Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Gene

ndufv1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • FMNCuratedNote: Binds 1 FMN.Curated
  • [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi396 – 3961Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi399 – 3991Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi402 – 4021Iron-sulfur (4Fe-4S)Sequence Analysis
Metal bindingi442 – 4421Iron-sulfur (4Fe-4S)Sequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi103 – 11210NAD(H)By similarity
Nucleotide bindingi216 – 26449FMNBy similarityAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Flavoprotein, FMN, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-51kD
Short name:
CI-51kD
NADH-ubiquinone oxidoreductase 51 kDa subunit
Gene namesi
Name:ndufv1
Synonyms:qinA, rcdG, veg107
ORF Names:DDB_G0288875
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0288875. ndufv1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 479NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrialPRO_0000327999
Transit peptidei1 – ?Mitochondrion

Proteomic databases

PRIDEiQ54I90.

Interactioni

Subunit structurei

Complex I is composed of about 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme (By similarity).By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191420.

Structurei

3D structure databases

ProteinModelPortaliQ54I90.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 51 kDa subunit family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1894.
InParanoidiQ54I90.
KOiK03942.
OMAiASWFSSI.
PhylomeDBiQ54I90.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54I90-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNLGNRVKS VIKLTSTTGL TGKDFQATKV ATFSTAQVQQ AQENVRSYGG
60 70 80 90 100
LKDKDRIFTN LYGEHDVYLK GAIARGDWYK TKNIIDKGKD WILKEMMASG
110 120 130 140 150
LRGRGGAGFP SGLKWSFMPK TTSKDRPQYL VINADEGEPG TCKDREIMRH
160 170 180 190 200
DPHKLIEGCL LAGFAMRACA AYIYIRGEFH YEAKVLEQAI DEAYKAGLIG
210 220 230 240 250
ENACGTGYKF DVYVHRGAGA YICGEETALI ESIEGKQGKP RLKPPFPAMA
260 270 280 290 300
GLYGCPTTVT NVETVAVAPT ILRRGGAWFA SFGRPKNAGT KLFCISGHVN
310 320 330 340 350
NPCTVEEEMS IPLRELIDKH CGGVIGGWDN LKGVIPGGSS VPVLPKNICD
360 370 380 390 400
NVLMDFDDLR QHRSGLGTAA VIVMNKETDM IAAIARLSKF YKHESCGQCT
410 420 430 440 450
PCREGVGWLY DITDRLVTGN AKPDEIDSLE EISRQIEGHT ICALGDAAAW
460 470
PVQGLIRHFR PEIEDRIKQF QLNKKQSPF
Length:479
Mass (Da):52,691
Last modified:May 24, 2005 - v1
Checksum:i1316E7409080C448
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241D → N in AAB03672 (PubMed:8643615).Curated
Sequence conflicti120 – 1201K → E in AAB03672 (PubMed:8643615).Curated
Sequence conflicti231 – 2311E → G in AAB03672 (PubMed:8643615).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61989 mRNA. Translation: AAB03672.1.
AAFI02000126 Genomic DNA. Translation: EAL62961.1.
RefSeqiXP_636489.1. XM_631397.1.

Genome annotation databases

EnsemblProtistsiDDB0191420; DDB0191420; DDB_G0288875.
GeneIDi8626872.
KEGGiddi:DDB_G0288875.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U61989 mRNA. Translation: AAB03672.1.
AAFI02000126 Genomic DNA. Translation: EAL62961.1.
RefSeqiXP_636489.1. XM_631397.1.

3D structure databases

ProteinModelPortaliQ54I90.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0191420.

Proteomic databases

PRIDEiQ54I90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0191420; DDB0191420; DDB_G0288875.
GeneIDi8626872.
KEGGiddi:DDB_G0288875.

Organism-specific databases

dictyBaseiDDB_G0288875. ndufv1.

Phylogenomic databases

eggNOGiCOG1894.
InParanoidiQ54I90.
KOiK03942.
OMAiASWFSSI.
PhylomeDBiQ54I90.

Miscellaneous databases

PROiQ54I90.

Family and domain databases

InterProiIPR001949. NADH-UbQ_OxRdtase_51kDa_CS.
IPR011537. NADH-UbQ_OxRdtase_suF.
IPR011538. Nuo51_FMN-bd.
IPR019575. Nuop51_4Fe4S-bd.
IPR019554. Soluble_ligand-bd.
[Graphical view]
PfamiPF01512. Complex1_51K. 1 hit.
PF10589. NADH_4Fe-4S. 1 hit.
PF10531. SLBB. 1 hit.
[Graphical view]
SMARTiSM00928. NADH_4Fe-4S. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01959. nuoF_fam. 1 hit.
PROSITEiPS00644. COMPLEX1_51K_1. 1 hit.
PS00645. COMPLEX1_51K_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome."
    Kuspa A., Loomis W.F.
    Proc. Natl. Acad. Sci. U.S.A. 93:5562-5566(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: AX4.
  2. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiNDUV1_DICDI
AccessioniPrimary (citable) accession number: Q54I90
Secondary accession number(s): Q23923
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 22, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.