Q54I80 (PANC_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantoate--beta-alanine ligase
EC=6.3.2.1

Alternative name(s):
Pantoate-activating enzyme
Pantothenate synthetase

Gene names

Name:	panC
ORF Names:	DDB_G0288935

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity.
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity.
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Pantoate--beta-alanine ligase		PRO_0000328042
Regions
	Nucleotide binding	43 – 50	8	ATP By similarity
	Nucleotide binding	162 – 165	4	ATP By similarity
	Nucleotide binding	199 – 202	4	ATP By similarity
Sites
	Active site	50	1	Proton donor By similarity
	Binding site	74	1	Beta-alanine By similarity
	Binding site	74	1	Pantoate By similarity
	Binding site	168	1	Pantoate By similarity
	Binding site	191	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54I80 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: DE906470A9BDCA46	FASTA	300	34,105
	10 20 30 40 50 60 MKELIICNNI KLIKEEIHKK KIEISKRNNK EYYEIKVGFV PTMGYLHSGH ISLVERAKLE 70 80 90 100 110 120 NDIVVVSIFV NPTQFNANED LSSYPSDIEN DSKLLKNVGT DLLFLPTPDI MYPKESGYST 130 140 150 160 170 180 FVTVESMEQV MEGKSRPGHF RGVATIVTKL LLITTPTNLY IGQKDAMQCI CIKRLVADLN 190 200 210 220 230 240 IDTNVIICNT IREDTGLAKS SRNSYLSNEE QIQASSIYKI LESFKNNINS FTDRQSFINE 250 260 270 280 290 300 ITKQLEQNPL FKVEYVSIAS NITGLEIIDQ FPPPKDSNLS LALLFFAEKR KTRLIDIIIL

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000126 Genomic DNA. Translation: EAL62991.1.
RefSeq	XP_636499.1. XM_631407.1.
3D structure databases
HSSP	HSSP built from PDB template 2A84 based on UniProtKB P0A5R0.
ProteinModelPortal	Q54I80.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231511.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231511; DDB0231511; DDB_G0288935.
GeneID	8626882.
KEGG	ddi:DDB_G0288935.
Organism-specific databases
dictyBase	DDB_G0288935. panC.
Phylogenomic databases
eggNOG	COG0414.
KO	K01918.
OMA	ICNTIRE.
ProtClustDB	CLSZ2429834.
Enzyme and pathway databases
UniPathway	UPA00028; UER00005.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR004821. Cyt_trans-like. IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR00018. panC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_DICDI

Accession

Primary (citable) accession number: Q54I80

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)