Q54I60 (PUR3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylglycinamide formyltransferase
EC=2.1.2.2

Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART

Gene names

Name:	purN
ORF Names:	DDB_G0288985

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro adenine biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: InterPro phosphoribosylglycinamide formyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Phosphoribosylglycinamide formyltransferase		PRO_0000327998
Regions
	Region	13 – 15	3	5'-phosphoribosylglycinamide binding By similarity
	Region	99 – 102	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	123	1	Proton donor By similarity
	Binding site	121	1	10-formyltetrahydrofolate By similarity
	Binding site	163	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	192	1	5'-phosphoribosylglycinamide By similarity
	Site	163	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54I60 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 0364CAE5C71DB559	FASTA	206	23,233
	10 20 30 40 50 60 MTFNICVLIS GNGTNLQAII DAIESKYLNV CIKVVISNKE TAYGLERAKK ASIETRVFSL 70 80 90 100 110 120 QKYLKQDPIN NTRSTYGLEL AKIIREYSSI DLIVLAGWMI ILPATFLKEF TDNKPTIDII 130 140 150 160 170 180 NLHPALPGQY PGAHAIERAF NDFKENKIKH SGIMIHKVIE EVDAGEVILT KEIPILPTDT 190 200 LESLEERFHQ QEHKSLVESI KLLSTK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000129 Genomic DNA. Translation: EAL62947.1.
RefSeq	XP_636450.1. XM_631358.1.
3D structure databases
HSSP	HSSP built from PDB template 1GAR based on UniProtKB P08179.
ProteinModelPortal	Q54I60.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54I60.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0230085; DDB0230085; DDB_G0288985.
GeneID	8626902.
GenomeReviews	Gene locus purN in contig CM000154_GR.
KEGG	ddi:DDB_G0288985.
Organism-specific databases
dictyBase	DDB_G0288985. purN.
Phylogenomic databases
eggNOG	KOG3076.
GeneTree	EPrGT00050000005314.
HOGENOM	HBG649382.
OMA	GSNMQAI.
PhylomeDB	Q54I60.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K00601.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_DICDI

Accession

Primary (citable) accession number: Q54I60

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)