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Q54I43 (UBE2S_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 S

EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S
Gene names
Name:ube2s
ORF Names:DDB_G0289021
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length215 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating polyubiquitin chains initiated by the E2 enzyme ubch10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Ubl conjugation pathway
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

ubiquitin-protein ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 215215Ubiquitin-conjugating enzyme E2 S
PRO_0000327752

Regions

Compositional bias162 – 1676Poly-Asn
Compositional bias169 – 18012Poly-Thr

Sites

Active site931Glycyl thioester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54I43 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 8D79DBBEBAD4519C

FASTA21523,617
        10         20         30         40         50         60 
MSSENLPPDV IKRVVKELKE LNSSTLEGIT LLPCEEDITN IEAVVTGPAG TPYEGGYFKA 

        70         80         90        100        110        120 
RLILSSDFPR SPPKANFITK IFHPNVSKKG EICVNTLKKD WTEDLGLKHI LLTIKCLLIV 

       130        140        150        160        170        180 
PNAESSLNED ASRLLLENYD DYCKHAKLFT SIHASKPIID SNNNNENSTT TPTTTTTATT 

       190        200        210 
PSTNTASISS PVKKKTETTN STTTKVQPKK SLKRL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000129 Genomic DNA. Translation: EAL62926.1.

3D structure databases

ProteinModelPortalQ54I43.
SMRQ54I43. Positions 4-154.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0188215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0305006; DDB0305006; DDB_G0289021.
KEGGddi:DDB_G0289021.

Organism-specific databases

dictyBaseDDB_G0289021. ube2s.

Phylogenomic databases

eggNOGCOG5078.
KOK10583.
OMAPDLGIKH.
PhylomeDBQ54I43.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54I43.

Entry information

Entry nameUBE2S_DICDI
AccessionPrimary (citable) accession number: Q54I43
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase