Reviewed, UniProtKB/Swiss-Prot Q54I36 (PYK3_DICDI)

Names and origin

Protein names

Recommended name:
    Dual specificity protein kinase pyk3
    EC=2.7.12.1

Gene names

Name:	pyk3
Synonyms:	DpyK3, pkyA
ORF Names:	DDB_G0289001

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1338 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the development. Negatively regulates tyrosine phosphorylation and activation state of dstC/STATc, probably by activating a dstC/STATc phosphatase. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Developmental stage	Expression is initially low during the vegetative stage, but increases after 5 and 15 hours of development. Up-regulation of the expression at the postaggregative stages is observed. Ref.3
Domain	The truncated N-terminal protein kinase domain lacks most of its classical characteristics and in vitro, has no activity. The protein kinase domain 1 is predicted to be catalytically inactive.
Post-translational modification	C-terminal tyrosine kinase domain is capable of autophosphorylation, in vitro.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Tyr protein kinase family. Contains 2 protein kinase domains.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid autophosphorylation Ref.1 Inferred from direct assay. Source: dictyBase regulation of sorocarp development Ref.3 Inferred from mutant phenotype. Source: dictyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein tyrosine kinase activity Ref.1 Inferred from direct assay. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1338	1338	Dual specificity protein kinase pyk3		PRO_0000354056
Regions
	Domain	693 – 1014	322	Protein kinase 1
	Domain	1057 – 1309	253	Protein kinase 2
	Nucleotide binding	699 – 707	9	ATP By similarity
	Nucleotide binding	1063 – 1071	9	ATP By similarity
	Compositional bias	5 – 22	18	Poly-Asn
	Compositional bias	151 – 157	7	Poly-Gln
	Compositional bias	199 – 208	10	Poly-His
	Compositional bias	276 – 283	8	Poly-Asn
	Compositional bias	349 – 365	17	Poly-Thr
	Compositional bias	376 – 379	4	Poly-Ser
	Compositional bias	448 – 461	14	Poly-His
	Compositional bias	657 – 665	9	Poly-Gly
Sites
	Active site	1174	1	Proton acceptor By similarity
	Binding site	727	1	ATP By similarity
	Binding site	1084	1	ATP By similarity
Experimental info
	Sequence conflict	917 – 920	4	APEM → GTET in AAB04169. Ref.1
	Sequence conflict	979	1	I → F in AAB04169. Ref.1
	Sequence conflict	979	1	I → F in AAB04999. Ref.1
	Sequence conflict	1009	1	E → G in AAB04999. Ref.1
	Sequence conflict	1021	1	W → R in AAB04169. Ref.1
	Sequence conflict	1062	1	K → R in AAB04999. Ref.1
	Sequence conflict	1072	1	F → S in AAB04999. Ref.1
	Sequence conflict	1119	1	C → R in AAB04169. Ref.1
	Sequence conflict	1119	1	C → R in AAB04999. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q54I36-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 029B57F88678BE48	FASTA	1,338	149,944
	10 20 30 40 50 60 MDSFNNNNNN NNNNNNNNNN INGEGITLRT LLNSCTNTSS NEQLIVNNVN KNSNIINNIN 70 80 90 100 110 120 INNTPSPIST CINVNKIELR GSSNGIFLNS KIKVPLPNST LLEQQQDGAD EQDKKQQSLS 130 140 150 160 170 180 DKNILFSSGD KEFLNHGSNN IITDQNTLLY QLQQQQQKEK EKENDEIMNH DDIIGYNEEN 190 200 210 220 230 240 EDNFFNEGMD PILAHSIEHH LHNHHHHHGE FDTQENEDTS GESSDESENI DEVLVYTEDI 250 260 270 280 290 300 ESEKEKKRER LITSPPSFDP HTLYSMSQSL LNCSLNNNNN NNNSISSPSS SINNSGNNIN 310 320 330 340 350 360 LNNSGNNNVN SNNNTNIINN SNDNFISQPL FNPLSMPNNE QYELLPNPTT TTSTITSTTT 370 380 390 400 410 420 TTTITNLPPA LPSFPSSSSI KSLKNSFGSN SITSSGELNN VFSSSMSPPM SPPNRNVRSL 430 440 450 460 470 480 TFPGTNPINC INTSVINSIT ANTNCINHHH HHHQHNHHNH HQGHNNINNS GHIRKSADDT 490 500 510 520 530 540 VTLSPTLSSG SSSTSSSNPH HPNHNHQKGL NNKTLEKLSC TRKEIYELIE KKESLIEKQN 550 560 570 580 590 600 LIDEGYSENA DSFENLSEEI QKINEKIIEL ENLITSLSNS NSNWSLNGSS TSTISCNPLS 610 620 630 640 650 660 PRSMNPSSST SSTSSNLTNS LRKFSQELKI ELRPLDLRAE LYSSINTSPR GSASISGGSG 670 680 690 700 710 720 SGGGGNNNGC FKTSSNSSIN SPIQFFENEN ESIDSYEKKN EEQFESLTQL IRENQLYTKP 730 740 750 760 770 780 IEFKEIKLLE KLESNSKSSN IWQIEYKSTQ LVLKQPKDQD SDKNIEKRKQ LFNGSNVSGS 790 800 810 820 830 840 NNSGSSGGNN HNHHHCNNSN GSNSEVIPSK YTMIQHKNLG LLVGWCGDSI IFESFKGMNS 850 860 870 880 890 900 LHDLIHRDGL KIDMALFIKI SKDIASVMGL LHSKDVAHGN LTSRSIYLDR FQIVKVSFPK 910 920 930 940 950 960 LNATDLNNPA IEPRYMAPEM TRMEEDQISC SIDVYAYAFV LWEALTSHLP FRKFNDISVA 970 980 990 1000 1010 1020 AKVAYENLRP KIPTSCPLII RKLINRCWAP LPSDRPTFND ILKLFDHLEG KLFFSSPGIL 1030 1040 1050 1060 1070 1080 WSLNNDQEVE RELQKKERFN EITEFLRGKK EIKFDEVAIV EKVGAGSFAN VFLGIWNGYK 1090 1100 1110 1120 1130 1140 VAIKILKNES ISNDEKFIKE VSSLIKSHHP NVVTFMGACI DPPCIFTEYL QGGSLYDVLH 1150 1160 1170 1180 1190 1200 IQKIKLNPLM MYKMIHDLSL GMEHLHSIQM LHRDLTSKNI LLDEFKNIKI ADFGLATTLS 1210 1220 1230 1240 1250 1260 DDMTLSGITN PRWRSPELTK GLVYNEKVDV YSFGLVVYEI YTGKIPFEGL DGTASAAKAA 1270 1280 1290 1300 1310 1320 FENYRPAIPP DCPVSLRKLI TKCWASDPSQ RPSFTEILTE LETMKSKFIK QLSFLNDLIQ 1330 NPDDDYNNNL NYDEEVDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Classification of tyrosine kinases from Dictyostelium discoideum with two distinct, complete or incomplete catalytic domains." Adler K., Gerisch G., von Hugo U., Lupas A., Schweiger A. FEBS Lett. 395:286-292(1996) [PubMed: 8898113] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AUTOPHOSPHORYLATION. Strain: AX2.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Dictyostelium kinase DPYK3 negatively regulates STATc signaling in cell fate decision." Lee N.-S., Rodriguez M., Kim B., Kim L. Dev. Growth Differ. 50:607-613(2008) [PubMed: 18657170] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01064 mRNA. Translation: AAB04169.1. U64830 Genomic DNA. Translation: AAB04999.1. AAFI02000129 Genomic DNA. Translation: EAL62916.1.
PIR	T18287.
RefSeq	XP_636435.1.
3D structure databases
HSSP	HSSP built from PDB template 2EVA based on UniProtKB O43318.
SMR	Q54I36. Positions 721-1008, 980-1306, 1051-1308.
ModBase	Search...
Genome annotation databases
GeneID	8626928.
KEGG	ddi:DDB_0191218.
Organism-specific databases
dictyBase	DDB_G0289001. pky3.
Phylogenomic databases
eggNOG	KOG0192.
PhylomeDB	Q54I36.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008266. Tyr_prot_kinase_AS. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYK3_DICDI

Accession

Primary (citable) accession number: Q54I36
Secondary accession number(s): Q23846, Q23927

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)