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Q54HY0 (PDE7_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7

EC=3.1.4.35
EC=3.1.4.53
Alternative name(s):
Phosphodiesterase 7
Short name=ddPDE7
Gene names
Name:pde7
ORF Names:DDB_G0289145
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Phosphodiesterase with dual cAMP/cGMP specificity. However, displays a preference for cAMP over cGMP. Seems to regulate cAMP/cGMP concentration especially during cell aggregation.

Catalytic activity

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Enzyme regulation

Inhibited by dithiotreitol (DTT). Ref.2

Subcellular location

Secretedextracellular space. Cell surface Ref.2.

Induction

Down-regulated by growth on bacteria. Ref.2

Sequence similarities

Belongs to the cAMP phosphodiesterase class-II family.

Biophysicochemical properties

Kinetic parameters:

KM=12.5 µM for cAMP Ref.2

KM=36 µM for cGMP

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 425408cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7
PRO_0000363968

Sequences

Sequence LengthMass (Da)Tools
Q54HY0 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: ADE7F8A5D17D3713

FASTA42548,505
        10         20         30         40         50         60 
MKYLILILIF FIEINNGSRL INSGNLFSEL KDYYIPENLN YYSGGYSEQH CKDSSYITIP 

        70         80         90        100        110        120 
LGVTGGLDEG SLSSFLLTKK GSSLFIGLDA GTVWQGVRRL TMLQDFNSVF NITYPPWATL 

       130        140        150        160        170        180 
PEQRATWFIK NHIQGYLIGH SHLDHVGGLI VESAEDQLSP KKNELEVSQP EIYRGCIEMI 

       190        200        210        220        230        240 
HKMGYVSDFP NITSIPDQKK PIIGINETLY SMATDLFNGF VWPSLPNYGR YSYYYLGNGN 

       250        260        270        280        290        300 
QYSFKDLTPY ANKYVTKVQN DFPFNHLVKS FEICHDSLTS TAFILTDSQS GEQIVFFSDT 

       310        320        330        340        350        360 
GISTTKCDWE FKILQVWRNI KIDKLKAVYI ESSFTNEVAD NVLFGHLRPK DIMKLMDSLL 

       370        380        390        400        410        420 
ENSIQTSPPK TNLKHVKLII EHIKPQVGMN QYYLTSQRMV YQQLQEINNH GVKVIIPNQG 


VPICL 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP."
Bader S., Kortholt A., Van Haastert P.J.M.
Biochem. J. 402:153-161(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION.
[3]"Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000130 Genomic DNA. Translation: EAL62880.1.
RefSeqXP_636383.1. XM_631291.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0188281.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0238626; DDB0238626; DDB_G0289145.
GeneID8626984.
KEGGddi:DDB_G0289145.

Organism-specific databases

dictyBaseDDB_G0289145. pde7.

Phylogenomic databases

eggNOGCOG5212.
OMADIMKLMD.
PhylomeDBQ54HY0.

Family and domain databases

Gene3D3.60.15.10. 3 hits.
InterProIPR001279. Beta-lactamas-like.
IPR024225. cAMP-PdiesteraseII_CS.
IPR000396. Pdiesterase2.
[Graphical view]
PfamPF02112. PDEase_II. 1 hit.
[Graphical view]
PIRSFPIRSF000962. Cyc_nuc_PDEase. 1 hit.
PRINTSPR00388. PDIESTERASE2.
SUPFAMSSF56281. SSF56281. 2 hits.
PROSITEPS00607. PDEASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDE7_DICDI
AccessionPrimary (citable) accession number: Q54HY0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: May 24, 2005
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase