Q54HY0 (PDE7_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7
EC=3.1.4.35
EC=3.1.4.53

Alternative name(s):
Phosphodiesterase 7
Short name=ddPDE7

Gene names

Name:	pde7
ORF Names:	DDB_G0289145

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phosphodiesterase with dual cAMP/cGMP specificity. However, displays a preference for cAMP over cGMP. Seems to regulate cAMP/cGMP concentration especially during cell aggregation.
Catalytic activity	Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate. Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Enzyme regulation	Inhibited by dithiotreitol (DTT). Ref.2
Subcellular location	Secreted › extracellular space. Cell surface Ref.2.
Induction	Down-regulated by growth on bacteria. Ref.2 Ref.3
Sequence similarities	Belongs to the cAMP phosphodiesterase class-II family.
Biophysicochemical properties	Kinetic parameters: KM=12.5 µM for cAMP Ref.2 KM=36 µM for cGMP

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Nucleotide-binding cAMP cAMP-binding cGMP cGMP-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cAMP catabolic process Inferred from genetic interaction Ref.2. Source: dictyBase cGMP catabolic process Inferred from genetic interaction Ref.2. Source: dictyBase
Cellular component	cell surface Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-AMP phosphodiesterase activity Inferred from genetic interaction Ref.2. Source: dictyBase 3',5'-cyclic-GMP phosphodiesterase activity Inferred from genetic interaction Ref.2. Source: dictyBase cAMP binding Inferred from electronic annotation. Source: UniProtKB-KW cGMP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 425	408	cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7		PRO_0000363968

Sequences

	Sequence		Length	Mass (Da)
	Q54HY0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: ADE7F8A5D17D3713	FASTA	425	48,505
	10 20 30 40 50 60 MKYLILILIF FIEINNGSRL INSGNLFSEL KDYYIPENLN YYSGGYSEQH CKDSSYITIP 70 80 90 100 110 120 LGVTGGLDEG SLSSFLLTKK GSSLFIGLDA GTVWQGVRRL TMLQDFNSVF NITYPPWATL 130 140 150 160 170 180 PEQRATWFIK NHIQGYLIGH SHLDHVGGLI VESAEDQLSP KKNELEVSQP EIYRGCIEMI 190 200 210 220 230 240 HKMGYVSDFP NITSIPDQKK PIIGINETLY SMATDLFNGF VWPSLPNYGR YSYYYLGNGN 250 260 270 280 290 300 QYSFKDLTPY ANKYVTKVQN DFPFNHLVKS FEICHDSLTS TAFILTDSQS GEQIVFFSDT 310 320 330 340 350 360 GISTTKCDWE FKILQVWRNI KIDKLKAVYI ESSFTNEVAD NVLFGHLRPK DIMKLMDSLL 370 380 390 400 410 420 ENSIQTSPPK TNLKHVKLII EHIKPQVGMN QYYLTSQRMV YQQLQEINNH GVKVIIPNQG VPICL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP." Bader S., Kortholt A., Van Haastert P.J.M. Biochem. J. 402:153-161(2007) [PubMed: 17040207] [Abstract] Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION.
[3]	"Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium." Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S. BMC Genomics 9:291-291(2008) [PubMed: 18559084] [Abstract] Cited for: INDUCTION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000130 Genomic DNA. Translation: EAL62880.1.
RefSeq	XP_636383.1. XM_631291.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0238626; DDB0238626; DDB_G0289145.
GeneID	8626984.
GenomeReviews	Gene locus pde7 in contig CM000154_GR.
KEGG	ddi:DDB_G0289145.
Organism-specific databases
dictyBase	DDB_G0289145. pde7.
Phylogenomic databases
eggNOG	euNOG09520.
GeneTree	EPrGT00050000005437.
HOGENOM	HBG082131.
ProtClustDB	CLSZ2429813.
Family and domain databases
InterPro	IPR024225. cAMP-PdiesteraseII_CS. IPR000396. Pdiesterase2. [Graphical view]
Pfam	PF02112. PDEase_II. 1 hit. [Graphical view]
PIRSF	PIRSF000962. Cyc_nuc_PDEase. 1 hit.
PRINTS	PR00388. PDIESTERASE2.
PROSITE	PS00607. PDEASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE7_DICDI

Accession

Primary (citable) accession number: Q54HY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)