Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q54HY0

- PDE7_DICDI

UniProt

Q54HY0 - PDE7_DICDI

Protein

cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7

Gene

pde7

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Phosphodiesterase with dual cAMP/cGMP specificity. However, displays a preference for cAMP over cGMP. Seems to regulate cAMP/cGMP concentration especially during cell aggregation.

    Catalytic activityi

    Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Enzyme regulationi

    Inhibited by dithiotreitol (DTT).1 Publication

    Kineticsi

    1. KM=12.5 µM for cAMP1 Publication
    2. KM=36 µM for cGMP1 Publication

    GO - Molecular functioni

    1. 3',5'-cyclic-AMP phosphodiesterase activity Source: dictyBase
    2. 3',5'-cyclic-GMP phosphodiesterase activity Source: dictyBase
    3. cAMP binding Source: UniProtKB-KW
    4. cGMP binding Source: UniProtKB-KW

    GO - Biological processi

    1. cAMP catabolic process Source: dictyBase
    2. cGMP catabolic process Source: dictyBase

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, cAMP-binding, cGMP, cGMP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7 (EC:3.1.4.35, EC:3.1.4.53)
    Alternative name(s):
    Phosphodiesterase 7
    Short name:
    ddPDE7
    Gene namesi
    Name:pde7
    ORF Names:DDB_G0289145
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0289145. pde7.

    Subcellular locationi

    Secretedextracellular space 1 Publication. Cell surface 1 Publication

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell
    2. extracellular region Source: dictyBase
    3. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 425408cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7PRO_0000363968Add
    BLAST

    Expressioni

    Inductioni

    Down-regulated by growth on bacteria.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0188281.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5212.
    OMAiDIMKLMD.
    PhylomeDBiQ54HY0.

    Family and domain databases

    Gene3Di3.60.15.10. 3 hits.
    InterProiIPR001279. Beta-lactamas-like.
    IPR024225. cAMP-PdiesteraseII_CS.
    IPR000396. Pdiesterase2.
    [Graphical view]
    PfamiPF02112. PDEase_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000962. Cyc_nuc_PDEase. 1 hit.
    PRINTSiPR00388. PDIESTERASE2.
    SUPFAMiSSF56281. SSF56281. 2 hits.
    PROSITEiPS00607. PDEASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54HY0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKYLILILIF FIEINNGSRL INSGNLFSEL KDYYIPENLN YYSGGYSEQH    50
    CKDSSYITIP LGVTGGLDEG SLSSFLLTKK GSSLFIGLDA GTVWQGVRRL 100
    TMLQDFNSVF NITYPPWATL PEQRATWFIK NHIQGYLIGH SHLDHVGGLI 150
    VESAEDQLSP KKNELEVSQP EIYRGCIEMI HKMGYVSDFP NITSIPDQKK 200
    PIIGINETLY SMATDLFNGF VWPSLPNYGR YSYYYLGNGN QYSFKDLTPY 250
    ANKYVTKVQN DFPFNHLVKS FEICHDSLTS TAFILTDSQS GEQIVFFSDT 300
    GISTTKCDWE FKILQVWRNI KIDKLKAVYI ESSFTNEVAD NVLFGHLRPK 350
    DIMKLMDSLL ENSIQTSPPK TNLKHVKLII EHIKPQVGMN QYYLTSQRMV 400
    YQQLQEINNH GVKVIIPNQG VPICL 425
    Length:425
    Mass (Da):48,505
    Last modified:May 24, 2005 - v1
    Checksum:iADE7F8A5D17D3713
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000130 Genomic DNA. Translation: EAL62880.1.
    RefSeqiXP_636383.1. XM_631291.1.

    Genome annotation databases

    EnsemblProtistsiDDB0238626; DDB0238626; DDB_G0289145.
    GeneIDi8626984.
    KEGGiddi:DDB_G0289145.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000130 Genomic DNA. Translation: EAL62880.1 .
    RefSeqi XP_636383.1. XM_631291.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0188281.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0238626 ; DDB0238626 ; DDB_G0289145 .
    GeneIDi 8626984.
    KEGGi ddi:DDB_G0289145.

    Organism-specific databases

    dictyBasei DDB_G0289145. pde7.

    Phylogenomic databases

    eggNOGi COG5212.
    OMAi DIMKLMD.
    PhylomeDBi Q54HY0.

    Family and domain databases

    Gene3Di 3.60.15.10. 3 hits.
    InterProi IPR001279. Beta-lactamas-like.
    IPR024225. cAMP-PdiesteraseII_CS.
    IPR000396. Pdiesterase2.
    [Graphical view ]
    Pfami PF02112. PDEase_II. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000962. Cyc_nuc_PDEase. 1 hit.
    PRINTSi PR00388. PDIESTERASE2.
    SUPFAMi SSF56281. SSF56281. 2 hits.
    PROSITEi PS00607. PDEASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP."
      Bader S., Kortholt A., Van Haastert P.J.M.
      Biochem. J. 402:153-161(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION.
    3. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
      Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
      BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPDE7_DICDI
    AccessioniPrimary (citable) accession number: Q54HY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3