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Protein

cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7

Gene

pde7

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphodiesterase with dual cAMP/cGMP specificity. However, displays a preference for cAMP over cGMP. Seems to regulate cAMP/cGMP concentration especially during cell aggregation.

Catalytic activityi

Adenosine 3',5'-cyclic phosphate + H2O = adenosine 5'-phosphate.
Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Enzyme regulationi

Inhibited by dithiotreitol (DTT).1 Publication

Kineticsi

  1. KM=12.5 µM for cAMP1 Publication
  2. KM=36 µM for cGMP1 Publication

    GO - Molecular functioni

    • 3',5'-cyclic-AMP phosphodiesterase activity Source: dictyBase
    • 3',5'-cyclic-GMP phosphodiesterase activity Source: dictyBase
    • cAMP binding Source: UniProtKB-KW
    • cGMP binding Source: UniProtKB-KW

    GO - Biological processi

    • cAMP catabolic process Source: dictyBase
    • cGMP catabolic process Source: dictyBase
    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cAMP, cAMP-binding, cGMP, cGMP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7 (EC:3.1.4.35, EC:3.1.4.53)
    Alternative name(s):
    Phosphodiesterase 7
    Short name:
    ddPDE7
    Gene namesi
    Name:pde7
    ORF Names:DDB_G0289145
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0289145. pde7.

    Subcellular locationi

    GO - Cellular componenti

    • cell surface Source: UniProtKB-SubCell
    • extracellular region Source: dictyBase
    • extracellular space Source: UniProtKB-SubCell
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 425408cAMP/cGMP-dependent 3',5'-cAMP/cGMP phosphodiesterase 7PRO_0000363968Add
    BLAST

    Expressioni

    Inductioni

    Down-regulated by growth on bacteria.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB0238626.

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5212.
    InParanoidiQ54HY0.
    OMAiDIMKLMD.
    PhylomeDBiQ54HY0.

    Family and domain databases

    Gene3Di3.60.15.10. 3 hits.
    InterProiIPR001279. Beta-lactamas-like.
    IPR024225. cAMP-PdiesteraseII_CS.
    IPR000396. Pdiesterase2.
    [Graphical view]
    PfamiPF02112. PDEase_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000962. Cyc_nuc_PDEase. 1 hit.
    PRINTSiPR00388. PDIESTERASE2.
    SUPFAMiSSF56281. SSF56281. 2 hits.
    PROSITEiPS00607. PDEASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54HY0-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKYLILILIF FIEINNGSRL INSGNLFSEL KDYYIPENLN YYSGGYSEQH
    60 70 80 90 100
    CKDSSYITIP LGVTGGLDEG SLSSFLLTKK GSSLFIGLDA GTVWQGVRRL
    110 120 130 140 150
    TMLQDFNSVF NITYPPWATL PEQRATWFIK NHIQGYLIGH SHLDHVGGLI
    160 170 180 190 200
    VESAEDQLSP KKNELEVSQP EIYRGCIEMI HKMGYVSDFP NITSIPDQKK
    210 220 230 240 250
    PIIGINETLY SMATDLFNGF VWPSLPNYGR YSYYYLGNGN QYSFKDLTPY
    260 270 280 290 300
    ANKYVTKVQN DFPFNHLVKS FEICHDSLTS TAFILTDSQS GEQIVFFSDT
    310 320 330 340 350
    GISTTKCDWE FKILQVWRNI KIDKLKAVYI ESSFTNEVAD NVLFGHLRPK
    360 370 380 390 400
    DIMKLMDSLL ENSIQTSPPK TNLKHVKLII EHIKPQVGMN QYYLTSQRMV
    410 420
    YQQLQEINNH GVKVIIPNQG VPICL
    Length:425
    Mass (Da):48,505
    Last modified:May 24, 2005 - v1
    Checksum:iADE7F8A5D17D3713
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AAFI02000130 Genomic DNA. Translation: EAL62880.1.
    RefSeqiXP_636383.1. XM_631291.1.

    Genome annotation databases

    EnsemblProtistsiDDB0238626; DDB0238626; DDB_G0289145.
    GeneIDi8626984.
    KEGGiddi:DDB_G0289145.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AAFI02000130 Genomic DNA. Translation: EAL62880.1.
    RefSeqiXP_636383.1. XM_631291.1.

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi44689.DDB0238626.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblProtistsiDDB0238626; DDB0238626; DDB_G0289145.
    GeneIDi8626984.
    KEGGiddi:DDB_G0289145.

    Organism-specific databases

    dictyBaseiDDB_G0289145. pde7.

    Phylogenomic databases

    eggNOGiCOG5212.
    InParanoidiQ54HY0.
    OMAiDIMKLMD.
    PhylomeDBiQ54HY0.

    Miscellaneous databases

    PROiQ54HY0.

    Family and domain databases

    Gene3Di3.60.15.10. 3 hits.
    InterProiIPR001279. Beta-lactamas-like.
    IPR024225. cAMP-PdiesteraseII_CS.
    IPR000396. Pdiesterase2.
    [Graphical view]
    PfamiPF02112. PDEase_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000962. Cyc_nuc_PDEase. 1 hit.
    PRINTSiPR00388. PDIESTERASE2.
    SUPFAMiSSF56281. SSF56281. 2 hits.
    PROSITEiPS00607. PDEASE_II. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP."
      Bader S., Kortholt A., Van Haastert P.J.M.
      Biochem. J. 402:153-161(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION.
    3. "Genome-wide transcriptional changes induced by phagocytosis or growth on bacteria in Dictyostelium."
      Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B., Skelton J., Ivens A., Bozzaro S.
      BMC Genomics 9:291-291(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPDE7_DICDI
    AccessioniPrimary (citable) accession number: Q54HY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: May 24, 2005
    Last modified: June 24, 2015
    This is version 53 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.