Q54HS3 (SET1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase set1
EC=2.1.1.43

Alternative name(s):
Histone H3 lysine 4 methyltransferase
SET domain-containing protein 1

Gene names

Name:	set1
Synonyms:	H3K4
ORF Names:	DDB_G0289257

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Histone methyltransferase that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3. May act to regulate chromatin-mediated events. Ref.2
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus. Chromosome Probable Ref.2.
Disruption phenotype	Cells display unusually rapid development, characterized by precocious aggregation into multicellular aggregates, and completely lack mono-, di- and trimethylation of H3K4 ('Lys-5' of histone 3). Cells also induce premature differentiation. Ref.2
Sequence similarities	Contains 1 post-SET domain. Contains 1 SET domain.

Ontologies

Keywords
Cellular component	Chromosome Nucleus
Domain	Coiled coil
Ligand	S-adenosyl-L-methionine
Molecular function	Activator Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aggregation involved in sorocarp development Inferred from mutant phenotype Ref.2. Source: dictyBase regulation of gene expression, epigenetic Inferred from mutant phenotype Ref.2. Source: dictyBase
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred by curator Ref.2. Source: dictyBase
Molecular_function	histone methyltransferase activity (H3-K4 specific) Inferred from mutant phenotype Ref.2. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1486	1486	Histone-lysine N-methyltransferase set1		PRO_0000379483
Regions
	Domain	1346 – 1468	123	SET
	Domain	1470 – 1486	17	Post-SET
	Coiled coil	359 – 400	42	Potential
	Coiled coil	717 – 744	28	Potential
	Coiled coil	1177 – 1255	79	Potential
	Compositional bias	30 – 44	15	Poly-Asn
	Compositional bias	45 – 50	6	Poly-Thr
	Compositional bias	116 – 140	25	Thr-rich
	Compositional bias	143 – 148	6	Poly-Asn
	Compositional bias	225 – 237	13	Poly-Thr
	Compositional bias	321 – 332	12	Poly-Pro
	Compositional bias	335 – 340	6	Poly-Pro
	Compositional bias	360 – 392	33	Poly-Gln
	Compositional bias	455 – 542	88	Arg-rich
	Compositional bias	553 – 585	33	Thr-rich
	Compositional bias	619 – 625	7	Poly-Ser
	Compositional bias	627 – 662	36	Poly-Asn
	Compositional bias	884 – 889	6	Poly-Gln
	Compositional bias	912 – 915	4	Poly-Asn
	Compositional bias	916 – 930	15	Poly-Asp
	Compositional bias	958 – 962	5	Poly-Asp
	Compositional bias	963 – 979	17	Poly-His
	Compositional bias	1066 – 1076	11	Poly-Thr
	Compositional bias	1219 – 1223	5	Poly-Asn
	Compositional bias	1295 – 1307	13	Poly-Ser
Experimental info
	Mutagenesis	1425	1	N → Q: Loss of catalytic activity; when associated with Ala-1474. Ref.2
	Mutagenesis	1474	1	C → A: Loss of catalytic activity; when associated with Gln-1425. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q54HS3 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: F46F71F1A5DFBFFC	FASTA	1,486	170,527
	10 20 30 40 50 60 MENETIVDNS LNNKSNVNNS NNDINNSKSN NNNTNTNYNN NHNNTTTTTT INKTEEKQND 70 80 90 100 110 120 SPKDSEFEFL DELKGVDDQH HVFSSEDESY TNGNKKRKQT DTPLSPNQDL KKRSITSPTT 130 140 150 160 170 180 SPTTSTSTST STSTSTSTST IINNNNNNLK DKTKEEIEFI KHIRSQLVKP KFLKDKPNFP 190 200 210 220 230 240 LRSSGGNWIF VGKLPSLQST TTDNTTLMSP NNATTTNGSS SNISTTTTTT TTTTPTTKIL 250 260 270 280 290 300 YRVNGFLSDN ETIDSIEINF GDPRDRYEIE RLHSSRINNP FELPCVSFKN PLFIKSNIAK 310 320 330 340 350 360 DIGISNEYGG MNDSFEFSNQ PPPPSPPPPP PPTLPPPPPP TLPPQHSLEQ QSTKQQIFTQ 370 380 390 400 410 420 QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQIPKINQQH YSTQPSVLID DIYDPSNPTE 430 440 450 460 470 480 PISPHQDHYP NFIFSKLQRY EHLPTRNPIS QYDYRDRPRD WERDRDRDWE RDRDWERDRD 490 500 510 520 530 540 RERDRDRDRD WERDRDWERD RDWERDRDRD RDWERDRDRD WERDRDRDWE RDRERDRDRY 550 560 570 580 590 600 DRQTNFSPAP QSTTTSASTS STTSSTDKNS NNTTSTSVSA TTSTTKRKSK FSEPIEPSPF 610 620 630 640 650 660 AIQIPRDNIK INGNLINNSS SSSSSGNNNN NNNNNNNNNN NNNNNNNNNN NNNNNSNNNN 670 680 690 700 710 720 NNSDVKDIKD KLLKQFKIYD PVNVYMDESY WYIDFRSSES RERAIQVLNG SFIDTWKLNV 730 740 750 760 770 780 DNKKTNTINE ELQKQKQLEN DSNNNKPNNF NLLENERSLK EICKLLVATE LLSTSSKDIS 790 800 810 820 830 840 KNFIEAEILK TIKLLDSQRI DPLTQNSTII NNTTNTTTSN INNTSNNTTV TPIVTPKSII 850 860 870 880 890 900 SAPTSRDSPR GGRSSSTTTK KPSKLDLNGS GVPPTLKKLD TIKQQQQPQP PLSPLKRPPK 910 920 930 940 950 960 SHFYSDSEDD GNNNNDDDDD DDDDEDDDFD QELSPLHSSR DSKKNIKSII KKKPIYSDDD 970 980 990 1000 1010 1020 DDHYHHHNHH HNHHHHHHHD RSEVELYNES DLQVDVLDSD NENQDESDYH KSSDNFGHVE 1030 1040 1050 1060 1070 1080 LSDDDNEFDS LDTDQDLYDT EENDNGKKSN KRPRKSKFNG KSKKPTTTTS TTTTATKSKG 1090 1100 1110 1120 1130 1140 RSKKTTITTP THNIPVLDEI QSNLDDEDAS YVSMVMAADK DIKLLFSTKS EEGFEDSSQE 1150 1160 1170 1180 1190 1200 ILSTPTRTKP SRNRKERNLP FLDEEDDESF KQLPQPQQKQ EKQEKHEHKL KNKELKQKNN 1210 1220 1230 1240 1250 1260 EVIINKTEEH FSENLNGDNN NNNDKSENEN ENENENKNEN ENDNNNLNTS IDNINGVERR 1270 1280 1290 1300 1310 1320 SITGCARSEG YTRSDIQKLF KRKQVAPTGK RGAASSASSG SNSSSSSTAE SFETGGNLSK 1330 1340 1350 1360 1370 1380 SARSSRFDNR GFGSDPITLA SLKSRRKRIK FERSDIHDWG LFAMETISAK DMVIEYIGEV 1390 1400 1410 1420 1430 1440 IRQKVADERE KRYVKKGIGS SYLFRVDDDT IIDATFKGNL ARFINHCCDP NCIAKVLTIG 1450 1460 1470 1480 NQKKIIIYAK RDINIGEEIT YDYKFPIEDV KIPCLCKSPK CRQTLN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Developmental timing in Dictyostelium is regulated by the Set1 histone methyltransferase." Chubb J.R., Bloomfield G., Xu Q., Kaller M., Ivens A., Skelton J., Turner B.M., Nellen W., Shaulsky G., Kay R.R., Bickmore W.A., Singer R.H. Dev. Biol. 292:519-532(2006) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION, MUTAGENESIS OF ASN-1425 AND CYS-1474, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000132 Genomic DNA. Translation: EAL62816.1.
RefSeq	XP_636258.1. XM_631166.1.
3D structure databases
HSSP	HSSP built from PDB template 1PEG based on UniProtKB Q8X225.
ProteinModelPortal	Q54HS3.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0233375.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233375; DDB0233375; DDB_G0289257.
GeneID	8627040.
KEGG	ddi:DDB_G0289257.
Organism-specific databases
dictyBase	DDB_G0289257. set1.
Phylogenomic databases
eggNOG	COG2940.
InParanoid	Q54HS3.
KO	K11422.
OMA	WERDRDW.
Family and domain databases
InterPro	IPR015722. Histone-lysine_MeTfrase. IPR003616. Post-SET_dom. IPR001214. SET_dom. [Graphical view]
PANTHER	PTHR22884:SF10. PTHR22884:SF10. 1 hit.
Pfam	PF00856. SET. 1 hit. [Graphical view]
SMART	SM00508. PostSET. 1 hit. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS50868. POST_SET. 1 hit. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SET1_DICDI

Accession

Primary (citable) accession number: Q54HS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)