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Protein

Histone-lysine N-methyltransferase set1

Gene

set1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Histone methyltransferase that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3. May act to regulate chromatin-mediated events.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

GO - Molecular functioni

  • histone methyltransferase activity (H3-K4 specific) Source: dictyBase
  • nucleotide binding Source: InterPro

GO - Biological processi

  • aggregation involved in sorocarp development Source: dictyBase
  • histone methylation Source: dictyBase
  • regulation of gene expression, epigenetic Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase set1 (EC:2.1.1.43)
Alternative name(s):
Histone H3 lysine 4 methyltransferase
SET domain-containing protein 1
Gene namesi
Name:set1
Synonyms:H3K4
ORF Names:DDB_G0289257
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0289257. set1.

Subcellular locationi

GO - Cellular componenti

  • chromosome Source: UniProtKB-SubCell
  • nucleus Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Cells display unusually rapid development, characterized by precocious aggregation into multicellular aggregates, and completely lack mono-, di- and trimethylation of H3K4 ('Lys-5' of histone 3). Cells also induce premature differentiation.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1425N → Q: Loss of catalytic activity; when associated with Ala-1474. 1 Publication1
Mutagenesisi1474C → A: Loss of catalytic activity; when associated with Gln-1425. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003794831 – 1486Histone-lysine N-methyltransferase set1Add BLAST1486

Proteomic databases

PaxDbiQ54HS3.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0233375.

Structurei

3D structure databases

ProteinModelPortaliQ54HS3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1347 – 1464SETPROSITE-ProRule annotationAdd BLAST118
Domaini1470 – 1486Post-SETPROSITE-ProRule annotationAdd BLAST17

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili359 – 400Sequence analysisAdd BLAST42
Coiled coili717 – 744Sequence analysisAdd BLAST28
Coiled coili1177 – 1255Sequence analysisAdd BLAST79

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 44Poly-AsnAdd BLAST15
Compositional biasi45 – 50Poly-Thr6
Compositional biasi116 – 140Thr-richAdd BLAST25
Compositional biasi143 – 148Poly-Asn6
Compositional biasi225 – 237Poly-ThrAdd BLAST13
Compositional biasi321 – 332Poly-ProAdd BLAST12
Compositional biasi335 – 340Poly-Pro6
Compositional biasi360 – 392Poly-GlnAdd BLAST33
Compositional biasi455 – 542Arg-richAdd BLAST88
Compositional biasi553 – 585Thr-richAdd BLAST33
Compositional biasi619 – 625Poly-Ser7
Compositional biasi627 – 662Poly-AsnAdd BLAST36
Compositional biasi884 – 889Poly-Gln6
Compositional biasi912 – 915Poly-Asn4
Compositional biasi916 – 930Poly-AspAdd BLAST15
Compositional biasi958 – 962Poly-Asp5
Compositional biasi963 – 979Poly-HisAdd BLAST17
Compositional biasi1066 – 1076Poly-ThrAdd BLAST11
Compositional biasi1219 – 1223Poly-Asn5
Compositional biasi1295 – 1307Poly-SerAdd BLAST13

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily.PROSITE-ProRule annotation
Contains 1 post-SET domain.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1080. Eukaryota.
COG2940. LUCA.
InParanoidiQ54HS3.
KOiK11422.
OMAiWERDRDW.

Family and domain databases

InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR003616. Post-SET_dom.
IPR001214. SET_dom.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50868. POST_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54HS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENETIVDNS LNNKSNVNNS NNDINNSKSN NNNTNTNYNN NHNNTTTTTT
60 70 80 90 100
INKTEEKQND SPKDSEFEFL DELKGVDDQH HVFSSEDESY TNGNKKRKQT
110 120 130 140 150
DTPLSPNQDL KKRSITSPTT SPTTSTSTST STSTSTSTST IINNNNNNLK
160 170 180 190 200
DKTKEEIEFI KHIRSQLVKP KFLKDKPNFP LRSSGGNWIF VGKLPSLQST
210 220 230 240 250
TTDNTTLMSP NNATTTNGSS SNISTTTTTT TTTTPTTKIL YRVNGFLSDN
260 270 280 290 300
ETIDSIEINF GDPRDRYEIE RLHSSRINNP FELPCVSFKN PLFIKSNIAK
310 320 330 340 350
DIGISNEYGG MNDSFEFSNQ PPPPSPPPPP PPTLPPPPPP TLPPQHSLEQ
360 370 380 390 400
QSTKQQIFTQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQIPKINQQH
410 420 430 440 450
YSTQPSVLID DIYDPSNPTE PISPHQDHYP NFIFSKLQRY EHLPTRNPIS
460 470 480 490 500
QYDYRDRPRD WERDRDRDWE RDRDWERDRD RERDRDRDRD WERDRDWERD
510 520 530 540 550
RDWERDRDRD RDWERDRDRD WERDRDRDWE RDRERDRDRY DRQTNFSPAP
560 570 580 590 600
QSTTTSASTS STTSSTDKNS NNTTSTSVSA TTSTTKRKSK FSEPIEPSPF
610 620 630 640 650
AIQIPRDNIK INGNLINNSS SSSSSGNNNN NNNNNNNNNN NNNNNNNNNN
660 670 680 690 700
NNNNNSNNNN NNSDVKDIKD KLLKQFKIYD PVNVYMDESY WYIDFRSSES
710 720 730 740 750
RERAIQVLNG SFIDTWKLNV DNKKTNTINE ELQKQKQLEN DSNNNKPNNF
760 770 780 790 800
NLLENERSLK EICKLLVATE LLSTSSKDIS KNFIEAEILK TIKLLDSQRI
810 820 830 840 850
DPLTQNSTII NNTTNTTTSN INNTSNNTTV TPIVTPKSII SAPTSRDSPR
860 870 880 890 900
GGRSSSTTTK KPSKLDLNGS GVPPTLKKLD TIKQQQQPQP PLSPLKRPPK
910 920 930 940 950
SHFYSDSEDD GNNNNDDDDD DDDDEDDDFD QELSPLHSSR DSKKNIKSII
960 970 980 990 1000
KKKPIYSDDD DDHYHHHNHH HNHHHHHHHD RSEVELYNES DLQVDVLDSD
1010 1020 1030 1040 1050
NENQDESDYH KSSDNFGHVE LSDDDNEFDS LDTDQDLYDT EENDNGKKSN
1060 1070 1080 1090 1100
KRPRKSKFNG KSKKPTTTTS TTTTATKSKG RSKKTTITTP THNIPVLDEI
1110 1120 1130 1140 1150
QSNLDDEDAS YVSMVMAADK DIKLLFSTKS EEGFEDSSQE ILSTPTRTKP
1160 1170 1180 1190 1200
SRNRKERNLP FLDEEDDESF KQLPQPQQKQ EKQEKHEHKL KNKELKQKNN
1210 1220 1230 1240 1250
EVIINKTEEH FSENLNGDNN NNNDKSENEN ENENENKNEN ENDNNNLNTS
1260 1270 1280 1290 1300
IDNINGVERR SITGCARSEG YTRSDIQKLF KRKQVAPTGK RGAASSASSG
1310 1320 1330 1340 1350
SNSSSSSTAE SFETGGNLSK SARSSRFDNR GFGSDPITLA SLKSRRKRIK
1360 1370 1380 1390 1400
FERSDIHDWG LFAMETISAK DMVIEYIGEV IRQKVADERE KRYVKKGIGS
1410 1420 1430 1440 1450
SYLFRVDDDT IIDATFKGNL ARFINHCCDP NCIAKVLTIG NQKKIIIYAK
1460 1470 1480
RDINIGEEIT YDYKFPIEDV KIPCLCKSPK CRQTLN
Length:1,486
Mass (Da):170,527
Last modified:May 24, 2005 - v1
Checksum:iF46F71F1A5DFBFFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000132 Genomic DNA. Translation: EAL62816.1.
RefSeqiXP_636258.1. XM_631166.1.

Genome annotation databases

EnsemblProtistsiEAL62816; EAL62816; DDB_G0289257.
GeneIDi8627040.
KEGGiddi:DDB_G0289257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000132 Genomic DNA. Translation: EAL62816.1.
RefSeqiXP_636258.1. XM_631166.1.

3D structure databases

ProteinModelPortaliQ54HS3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0233375.

Proteomic databases

PaxDbiQ54HS3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL62816; EAL62816; DDB_G0289257.
GeneIDi8627040.
KEGGiddi:DDB_G0289257.

Organism-specific databases

dictyBaseiDDB_G0289257. set1.

Phylogenomic databases

eggNOGiKOG1080. Eukaryota.
COG2940. LUCA.
InParanoidiQ54HS3.
KOiK11422.
OMAiWERDRDW.

Miscellaneous databases

PROiQ54HS3.

Family and domain databases

InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR003616. Post-SET_dom.
IPR001214. SET_dom.
[Graphical view]
PfamiPF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00508. PostSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50868. POST_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSET1_DICDI
AccessioniPrimary (citable) accession number: Q54HS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: May 24, 2005
Last modified: November 2, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.