Q54HG9 (RPN2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit swp1
Short name=Oligosaccharyl transferase subunit swp1
EC=2.4.1.119

Alternative name(s):
Ribophorin II homolog

Gene names

Name:	swp1
ORF Names:	DDB_G0289479

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	685 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the SWP1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 685	667	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit swp1		PRO_0000328636
Regions
	Topological domain	19 – 590	572	Lumenal Potential
	Transmembrane	591 – 611	21	Helical; Potential
	Topological domain	612 – 620	9	Cytoplasmic Potential
	Transmembrane	621 – 641	21	Helical; Potential
	Topological domain	642 – 646	5	Lumenal Potential
	Transmembrane	647 – 667	21	Helical; Potential
	Topological domain	668 – 685	18	Cytoplasmic Potential
	Compositional bias	122 – 125	4	Poly-Ser
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	558	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54HG9 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 816D42023DC15FE4	FASTA	685	75,616
	10 20 30 40 50 60 MKLIILIVLS ILISIVISGS VQSKITTTRG ISNVYSKNDI NNIKQFISSK YNGNTKLYGE 70 80 90 100 110 120 SLKDTFYGVG VLTRIGETNI EATKDICKQT KEQLKQNKFT DIELVFNGVT ILSELKCLQG 130 140 150 160 170 180 ESSSSLGNEQ QLQDLLKNKL ENGSLLEKTQ AINIYFTLSS AKAIDSKTVS IIDPLLIQAV 190 200 210 220 230 240 NSMVSLMDED GTFKSVSTDD EGNLQNTAAA YFALARLSHR LKSNEVDKLV AKVVNKVDTV 250 260 270 280 290 300 LASADETTDS LYFNDLSTTS SLLHGLLSLA SVNDKVADVI SNKQINQISE YLLRQKNVES 310 320 330 340 350 360 LSDAYHLIVA LKRCQKNSIS QPISLALVKS IYSPSGLNDI RVRVTDIFDQ PIEASIVINK 370 380 390 400 410 420 VVSSKNPRST PILSGKEMKF QSSDNSFVAD LSNENLKLGS YNFEFKVQPV DTDSYKSITN 430 440 450 460 470 480 IQIITITGAV TVNDMKLSYA PKSDQLGSPK TTNEVQFGQK LPLIEIPSNN IARIFFRIAS 490 500 510 520 530 540 EAQPYQAQQV GIRFYSPARE AVVPATYSAD AYSYTFTNKD ACKILGCQSG NYQLDLIIGD 550 560 570 580 590 600 QSITPLQWNF GEINLKFNQS TIPTNRYPEQ LPISHNFRVA EKRPPQSISS LFTLLVLSPI 610 620 630 640 650 660 AIFVIGLLFV GTNLGRFPTG MGFIYTIGFL GCISATGLLI VNYWLHSTMD VTLKNLALLM 670 680 IPLVFFGHKS MSYYSNLSSS NIKKD

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000141 Genomic DNA. Translation: EAL62719.1.
RefSeq	XP_636216.1. XM_631124.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54HG9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233147; DDB0233147; DDB_G0289479.
GeneID	8627154.
GenomeReviews	Gene locus swp1 in contig CM000154_GR.
KEGG	ddi:DDB_G0289479.
Organism-specific databases
dictyBase	DDB_G0289479. swp1.
Phylogenomic databases
eggNOG	KOG2447.
OMA	PESSTFA.
PhylomeDB	Q54HG9.
ProtClustDB	CLSZ2728808.
Family and domain databases
InterPro	IPR008814. Ribophorin_II. [Graphical view]
PANTHER	PTHR12640. Ribophorin_II. 1 hit.
Pfam	PF05817. Ribophorin_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPN2_DICDI

Accession

Primary (citable) accession number: Q54HG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	September 21, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)