Q54HG7 (AUHM_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylglutaconyl-CoA hydratase, mitochondrial
EC=4.2.1.18

Alternative name(s):
AU-specific RNA-binding enoyl-CoA hydratase homolog
Short name=AU-binding protein/enoyl-CoA hydratase homolog

Gene names

Name:	auh
ORF Names:	DDB_G0289471

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3-methylglutaryl-CoA By similarity.
Catalytic activity	(S)-3-hydroxy-3-methylglutaryl-CoA = trans-3-methylglutaconyl-CoA + H2O.
Pathway	Amino-acid degradation; L-leucine degradation; (S)-3-hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 3/3.
Subunit structure	Homohexamer By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the enoyl-CoA hydratase/isomerase family.

Ontologies

Keywords
Biological process	Branched-chain amino acid catabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	enoyl-CoA hydratase activity Inferred from sequence or structural similarity. Source: UniProtKB mRNA 3'-UTR binding Inferred from sequence or structural similarity. Source: UniProtKB methylglutaconyl-CoA hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 303		Methylglutaconyl-CoA hydratase, mitochondrial		PRO_0000328210

Sequences

	Sequence		Length	Mass (Da)
	Q54HG7 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: A1D47C0A3EBBB2CB	FASTA	303	33,245
	10 20 30 40 50 60 MIKHLIKNVS NSVKYNNVTS NNILFSTSSS LKFGRKFTTE TQQECILERL EGENKGISVI 70 80 90 100 110 120 SFNRGHVKNA LGKNLMNQFR SHLNELRFCP DTRVVIVRSL VDGVFCSGAD LKERALMSQV 130 140 150 160 170 180 EASQFVHSLR SSFTELETLQ MPTIAAIEGV AVGGGTEMVL ACDFRVASKS SKMGLPETGL 190 200 210 220 230 240 AIIPGAGGTQ RLPRLIGIPR AKELIFTGAI LDSKRALEIG LVQYETEKGE AFDKAIEIAK 250 260 270 280 290 300 QIIPKGPIAI RMAKQAIDRG MNVDQASGMI IEQASYAQVI PTKDRIEGLT AFKEKRKPIY KGE

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000141 Genomic DNA. Translation: EAL62715.1.
RefSeq	XP_636218.1. XM_631126.1.
3D structure databases
HSSP	HSSP built from PDB template 1HZD based on UniProtKB Q13825.
ProteinModelPortal	Q54HG7.
SMR	Q54HG7. Positions 43-303.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54HG7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233850; DDB0233850; DDB_G0289471.
GeneID	8627156.
GenomeReviews	Gene locus auh in contig CM000154_GR.
KEGG	ddi:DDB_G0289471.
Organism-specific databases
dictyBase	DDB_G0289471. auh.
Phylogenomic databases
eggNOG	KOG1679.
GeneTree	EPrGT00050000004514.
HOGENOM	HBG748731.
OMA	EILPNGP.
PhylomeDB	Q54HG7.
ProtClustDB	CLSZ2429771.
Family and domain databases
InterPro	IPR014748. Crontonase_C. IPR001753. Crotonase_core. IPR018376. Enoyl-CoA_hyd/isom_CS. [Graphical view]
Gene3D	G3DSA:1.10.12.10. Crontonase_C. 1 hit.
KO	K05607.
Pfam	PF00378. ECH. 1 hit. [Graphical view]
PROSITE	PS00166. ENOYL_COA_HYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AUHM_DICDI

Accession

Primary (citable) accession number: Q54HG7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)