Q54GT6 (SPYA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine--pyruvate aminotransferase
Short name=SPT
EC=2.6.1.51

Alternative name(s):
Alanine--glyoxylate aminotransferase
Short name=AGT
EC=2.6.1.44

Gene names

Name:	agxt
ORF Names:	DDB_G0289923

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Dual metabolic roles of gluconeogenesis and glyoxylate detoxification By similarity.
Catalytic activity	L-serine + pyruvate = 3-hydroxypyruvate + L-alanine. L-alanine + glyoxylate = pyruvate + glycine.
Cofactor	Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glyoxylate metabolic process Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	alanine-glyoxylate transaminase activity Inferred from sequence or structural similarity. Source: dictyBase pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro serine-pyruvate transaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Serine--pyruvate aminotransferase		PRO_0000328587
Sites
	Binding site	376	1	Substrate By similarity
Amino acid modifications
	Modified residue	205	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54GT6 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: D5701FCFDB38DE50	FASTA	407	44,711
	10 20 30 40 50 60 MGYSDKHKLK TSNLQLPVRL LLGPGPSNLH PRVNQQLISS MVGYTDASYY KVMDETMELM 70 80 90 100 110 120 RYLFQTDNHF TIPISGAGTA AMETCVSNLI EPGDKVVCLV SGFFSDRIYQ MAVRYGGNIL 130 140 150 160 170 180 KVDKQWGQWF ALEEIESALV QHKPSLLTLV FGETSTGVKQ QMEGVGELCK KYNCLLMVDC 190 200 210 220 230 240 VAALGGVPVF VDDWKIDACY TGTQKCLSGP PGISPLTFSN AACHKIATRK TPVANWYLDC 250 260 270 280 290 300 NLLGGYWCPE FNLADGTIAK EEKSTIPKRY HHTTPANLLY SLREALVLLV EEGLDNVWKR 310 320 330 340 350 360 HQDAAILLYA GLESLGLKPF VPQLNEAPEK GGRLFSLTTV NIPDGVDGKM VMKYLLDNFN 370 380 390 400 IEIAGGIGAF AGKVWRIGLM GQNANPGNIK LLISSLSDAL KVNGFNC

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000149 Genomic DNA. Translation: EAL62474.1.
RefSeq	XP_635983.1. XM_630891.1.
3D structure databases
HSSP	HSSP built from PDB template 1H0C based on UniProtKB P21549.
ProteinModelPortal	Q54GT6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54GT6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0237978; DDB0237978; DDB_G0289923.
GeneID	8627398.
GenomeReviews	Gene locus agxt in contig CM000154_GR.
KEGG	ddi:DDB_G0289923.
Organism-specific databases
dictyBase	DDB_G0289923. agxt.
Phylogenomic databases
eggNOG	KOG2862.
HOGENOM	HBG700056.
OMA	MRQNVER.
PhylomeDB	Q54GT6.
ProtClustDB	CLSZ2429722.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR024169. SP_NH2Trfase/AEP_transaminase. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00830.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000524. SPT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPYA_DICDI

Accession

Primary (citable) accession number: Q54GT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)