Reviewed, UniProtKB/Swiss-Prot Q54GP3 (SPRE_DICDI)

Names and origin

Protein names

Recommended name:
    Sepiapterin reductase
      Short name=SPR
    EC=1.1.1.153

Gene names

Name:	sprA
ORF Names:	DDB_G0290009

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH. Ref.2 Tetrahydrobiopterin + 2 NADP+ = 6-pyruvoyl-5,6,7,8-tetrahydropterin + 2 NADPH. Ref.2
Enzyme regulation	Inhibited by N-acetylserotonin and to a lesser extent by melatonin.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	Dictyopterin, a D-threo isomer of biopterin, shown to exist in D.discoideum, was not identified in the enzymatic assay.
Sequence similarities	Belongs to the sepiapterin reductase family.
biophysicochemical properties	Kinetic parameters: KM=51.8 µM for NADPH KM=40 µM for sepiapterin Vmax=0.14 µmol/min/mg enzyme pH dependence: Optimum pH is 5.5. Temperature dependence: Optimum temperature is 30 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrobiopterin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro sepiapterin reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	Sepiapterin reductase		PRO_0000327640
Regions
	Nucleotide binding	9 – 38	30	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54GP3-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 46AB80622D788267	FASTA	267	30,187
	10 20 30 40 50 60 MSKVLAIVTG ASKGFGKAIA QEIVKCNPNK NQLIDLVLFA RSLDGLKSTK ESIETISTNA 70 80 90 100 110 120 KVIDLQSLDM SNIPDVELKF KSVLENIKWE EYSKICFFNN HGTLYHLGRI EDFSDFKNIQ 130 140 150 160 170 180 KDNDTNTTSF VVTTSLLVKK LKELGSQYSS EVTIVNSSSL CAIKAFPTWS LYCSSRAYRD 190 200 210 220 230 240 MFFQALSLEY KLNDKFKVLN YSLGVLDTDM QSQVREECSE DDKSFYVGLK NDGKLINPNR 250 260 SASICSNLVY SHKFETGSHL DYYDLDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Characterization of recombinant Dictyostelium discoideum sepiapterin reductase expressed in E. coli." Kim Y.-A., Chung H.J., Kim Y.J., Choi Y.K., Hwang Y.K., Lee S.W., Park Y.S. Mol. Cells 10:405-410(2000) [PubMed: 10987137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-267, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000151 Genomic DNA. Translation: EAL62410.1. AF253975 mRNA. Translation: AAF67740.1.
RefSeq	XP_635919.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3387494.
KEGG	ddi:DDB_0191275.
Organism-specific databases
dictyBase	DDB_G0290009. sprA.
Phylogenomic databases
OMA	Q54GP3. NRSASIC.
Enzyme and pathway databases
BRENDA	1.1.1.153. 424.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR006393. Sepiapterin_red. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01500. sepiapter_red. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SPRE_DICDI

Accession

Primary (citable) accession number: Q54GP3
Secondary accession number(s): Q9NGC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)