Q54GJ7 (HOT_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
Short name=HOT
EC=1.1.99.24

Gene names

Name:	adhfe1
ORF Names:	DDB_G0290111

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 4-hydroxybutanoate + 2-oxoglutarate = succinic semialdehyde + (R)-2-hydroxyglutarate.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 547		Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial		PRO_0000323002

Sequences

	Sequence		Length	Mass (Da)
	Q54GJ7 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: B03F1DF8B3B4DFC4	FASTA	547	59,743
	10 20 30 40 50 60 MTIIITKIAD KIIKSNRVKN TLQDIHIHSS NCGCHGSFKS NSVMNQTFSS FSSLTTEKNE 70 80 90 100 110 120 QQQQQLFKQF SNSKPIPMGR KVELDTSKVI DSVYFGNTEN NNYRTNKGNT DYAFEASANN 130 140 150 160 170 180 IRFGSGVTYE VGYDLLDMNC RNVIVFTDNN LLKLVDTDKE SAVAKCLYSL EKCGIKYTIY 190 200 210 220 230 240 SDVSIEPTDT SFKDAISVMG KGRYDGVVAV GGGSVMDTAK AANLYNSYPP ADNDFLAYIN 250 260 270 280 290 300 PPIGKGLLVP GPLKRPLIAI PTTCGTASET TGVCILDIKL GDGLSAKTGI ASRHLKPILG 310 320 330 340 350 360 LVDPDNLLTL PSNVAISSGF DQLCHALESF TAIPFNQRSP RPLAPNQRPS YQGANPVSDV 370 380 390 400 410 420 WSLRSLEMLC KNIHRFVLNP NDDYARSQMM LAASYAGLGF GNSGVHACHG MSYSISSMVK 430 440 450 460 470 480 DYKPEGYYGL KKNLIPHGQS VILSAPAVFK FTAPSNPERH LLLAKIMGAD ISNASESDAG 490 500 510 520 530 540 VLLSNQIVKL MKLLNVPNGL QALGYKESDI DSLVKGTLPQ HRVTKLMPKQ ATYDDLYKLF KDSMTIY

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000153 Genomic DNA. Translation: EAL62385.1.
RefSeq	XP_635889.1. XM_630797.1.
3D structure databases
ProteinModelPortal	Q54GJ7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54GJ7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0252839; DDB0252839; DDB_G0290111.
GeneID	8627488.
GenomeReviews	Gene locus adhfe1 in contig CM000154_GR.
KEGG	ddi:DDB_G0290111.
Organism-specific databases
dictyBase	DDB_G0290111. adhfe1.
Phylogenomic databases
eggNOG	KOG3857.
GeneTree	EPrGT00050000005929.
HOGENOM	HBG742415.
OMA	FEASANN.
Family and domain databases
InterPro	IPR001670. ADH_Fe. [Graphical view]
KO	K11173.
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOT_DICDI

Accession

Primary (citable) accession number: Q54GJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)