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Q54GJ2

- PUR2_DICDI

UniProt

Q54GJ2 - PUR2_DICDI

Protein

Bifunctional purine biosynthetic protein purD

Gene

purD

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
    ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi313 – 3131ManganeseBy similarity
    Metal bindingi315 – 3151ManganeseBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi139 – 20062ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. phosphoribosylamine-glycine ligase activity Source: dictyBase
    4. phosphoribosylformylglycinamidine cyclo-ligase activity Source: dictyBase

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleobase biosynthetic process Source: InterPro
    3. purine nucleobase metabolic process Source: dictyBase
    4. purine nucleotide biosynthetic process Source: dictyBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00125.
    UPA00074; UER00129.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional purine biosynthetic protein purD
    Including the following 2 domains:
    Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name:
    GARS
    Phosphoribosylglycinamide synthetase
    Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
    Alternative name(s):
    AIR synthase
    Short name:
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    Gene namesi
    Name:purD
    ORF Names:DDB_G0290121
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0290121. purD.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 815815Bifunctional purine biosynthetic protein purDPRO_0000328326Add
    BLAST

    Proteomic databases

    PRIDEiQ54GJ2.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0230084.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54GJ2.
    SMRiQ54GJ2. Positions 509-803.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini113 – 343231ATP-graspAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni5 – 452448GARSAdd
    BLAST
    Regioni467 – 808342AIRSAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the GARS family.Curated
    In the C-terminal section; belongs to the AIR synthase family.Curated
    Contains 1 ATP-grasp domain.Curated

    Phylogenomic databases

    eggNOGiCOG0151.
    KOiK11788.
    OMAiGMLFTGF.
    PhylomeDBiQ54GJ2.

    Family and domain databases

    Gene3Di3.30.1330.10. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPiMF_00138. GARS.
    InterProiIPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    TIGR00878. purM. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54GJ2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIKNNILVI GSGSREHAIT WKLLESNQVD KVILVPGNAS SSTMERVITV    50
    ECSKIDAESI SNICKEHNVE YVFVGPEVPL VDGIVDGLKR KGISCFGPTK 100
    KAAQLEGSKV FCKDFMARNN IPSARYQTFT DYNKAKQYIE SLNYKIVLKA 150
    SGCAAGKGVL IPNNKEEELE GLKRIMVDKE FGSAGDEIVI EEFLDGEECS 200
    LMCFSDGYSL VVMPPAQDHK RIFDGDKGAN TGGMGAYAPA PFIVDCNNNA 250
    TTDKSKSKSS FGTIIDRCVE TILKPTINGM RKEGKPFVGV LFAGLMVSSS 300
    SSTTNDKVIN VLEFNCRMGD PETQVVLPLL ETDLFEIVLA CIEGRLDGLD 350
    VKWSNKFAVT VVAASKGYPD SYPKGLKING LLENKNTNDN IIFQAGTTVN 400
    GSNDIVTNGG RVLSCTSVSE SLEDAIKNSY KLIETISFEG MQYRKDIGQK 450
    ALNHLERKKQ QQANSKQSVS YSESGVDIER GDAVVDNIGP LAKATTRLGC 500
    VSDLGGFGAL FDTKAAGFRD PILVSGTDGV GTKLKIAQEL GIHDSIGIDL 550
    VAMCVNDVVV QGAEPLFFLD YFATGRIHVD VATQVVSGIA RGCKESGCAL 600
    IGGETAEMPG MYKDGEYDLA GFSVGAVERD QMLPSNIQEG NILLGLASSG 650
    VHSNGYSLVR YLIETKSGGL TYNSIAPFDS SKTLGQVLLT PTKLYVLSCL 700
    AAIKSGGVNG LAHITGGGIT ENLPRVIPDG LDCEVELGSW EILPIFKYLV 750
    ELGNMETEEL LKTFNSGIGM ILIVSPDKVD SITKSLESNN EKVYKIGKII 800
    NSKTSKQSKS KVIYK 815
    Length:815
    Mass (Da):87,706
    Last modified:May 24, 2005 - v1
    Checksum:iC24A183386A06FC6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000153 Genomic DNA. Translation: EAL62377.1.
    RefSeqiXP_635881.1. XM_630789.1.

    Genome annotation databases

    EnsemblProtistsiDDB0230084; DDB0230084; DDB_G0290121.
    GeneIDi8627491.
    KEGGiddi:DDB_G0290121.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000153 Genomic DNA. Translation: EAL62377.1 .
    RefSeqi XP_635881.1. XM_630789.1.

    3D structure databases

    ProteinModelPortali Q54GJ2.
    SMRi Q54GJ2. Positions 509-803.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0230084.

    Proteomic databases

    PRIDEi Q54GJ2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0230084 ; DDB0230084 ; DDB_G0290121 .
    GeneIDi 8627491.
    KEGGi ddi:DDB_G0290121.

    Organism-specific databases

    dictyBasei DDB_G0290121. purD.

    Phylogenomic databases

    eggNOGi COG0151.
    KOi K11788.
    OMAi GMLFTGF.
    PhylomeDBi Q54GJ2.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    UPA00074 ; UER00129 .

    Miscellaneous databases

    PROi Q54GJ2.

    Family and domain databases

    Gene3Di 3.30.1330.10. 1 hit.
    3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    3.90.650.10. 1 hit.
    HAMAPi MF_00138. GARS.
    InterProi IPR010918. AIR_synth_C_dom.
    IPR000728. AIR_synth_N_dom.
    IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020562. PRibGlycinamide_synth_N.
    IPR004733. PurM_cligase.
    IPR016188. PurM_N-like.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF00586. AIRS. 1 hit.
    PF02769. AIRS_C. 1 hit.
    PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    SSF55326. SSF55326. 1 hit.
    SSF56042. SSF56042. 1 hit.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    TIGR00878. purM. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. Bienvenut W.V., Ura S., Insall R.H.
      Submitted (JUL-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 515-533 AND 667-682, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: AX2.

    Entry informationi

    Entry nameiPUR2_DICDI
    AccessioniPrimary (citable) accession number: Q54GJ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3