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Q54GJ2 (PUR2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthetic protein purD

Including the following 2 domains:

  1. Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name=GARS
    Phosphoribosylglycinamide synthetase
  2. Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
    Alternative name(s):
    AIR synthase
    Short name=AIRS
    Phosphoribosyl-aminoimidazole synthetase
Gene names
Name:purD
ORF Names:DDB_G0290121
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length815 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00138

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00138

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Sequence similarities

In the N-terminal section; belongs to the GARS family.

In the C-terminal section; belongs to the AIR synthase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 815815Bifunctional purine biosynthetic protein purD HAMAP-Rule MF_00138
PRO_0000328326

Regions

Domain113 – 343231ATP-grasp
Nucleotide binding139 – 20062ATP By similarity
Region5 – 452448GARS HAMAP-Rule MF_00138
Region467 – 808342AIRS HAMAP-Rule MF_00138

Sites

Metal binding3131Manganese By similarity
Metal binding3151Manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54GJ2 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: C24A183386A06FC6

FASTA81587,706
        10         20         30         40         50         60 
MTIKNNILVI GSGSREHAIT WKLLESNQVD KVILVPGNAS SSTMERVITV ECSKIDAESI 

        70         80         90        100        110        120 
SNICKEHNVE YVFVGPEVPL VDGIVDGLKR KGISCFGPTK KAAQLEGSKV FCKDFMARNN 

       130        140        150        160        170        180 
IPSARYQTFT DYNKAKQYIE SLNYKIVLKA SGCAAGKGVL IPNNKEEELE GLKRIMVDKE 

       190        200        210        220        230        240 
FGSAGDEIVI EEFLDGEECS LMCFSDGYSL VVMPPAQDHK RIFDGDKGAN TGGMGAYAPA 

       250        260        270        280        290        300 
PFIVDCNNNA TTDKSKSKSS FGTIIDRCVE TILKPTINGM RKEGKPFVGV LFAGLMVSSS 

       310        320        330        340        350        360 
SSTTNDKVIN VLEFNCRMGD PETQVVLPLL ETDLFEIVLA CIEGRLDGLD VKWSNKFAVT 

       370        380        390        400        410        420 
VVAASKGYPD SYPKGLKING LLENKNTNDN IIFQAGTTVN GSNDIVTNGG RVLSCTSVSE 

       430        440        450        460        470        480 
SLEDAIKNSY KLIETISFEG MQYRKDIGQK ALNHLERKKQ QQANSKQSVS YSESGVDIER 

       490        500        510        520        530        540 
GDAVVDNIGP LAKATTRLGC VSDLGGFGAL FDTKAAGFRD PILVSGTDGV GTKLKIAQEL 

       550        560        570        580        590        600 
GIHDSIGIDL VAMCVNDVVV QGAEPLFFLD YFATGRIHVD VATQVVSGIA RGCKESGCAL 

       610        620        630        640        650        660 
IGGETAEMPG MYKDGEYDLA GFSVGAVERD QMLPSNIQEG NILLGLASSG VHSNGYSLVR 

       670        680        690        700        710        720 
YLIETKSGGL TYNSIAPFDS SKTLGQVLLT PTKLYVLSCL AAIKSGGVNG LAHITGGGIT 

       730        740        750        760        770        780 
ENLPRVIPDG LDCEVELGSW EILPIFKYLV ELGNMETEEL LKTFNSGIGM ILIVSPDKVD 

       790        800        810 
SITKSLESNN EKVYKIGKII NSKTSKQSKS KVIYK 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]Bienvenut W.V., Ura S., Insall R.H.
Submitted (JUL-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 515-533 AND 667-682, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000153 Genomic DNA. Translation: EAL62377.1.
RefSeqXP_635881.1. XM_630789.1.

3D structure databases

ProteinModelPortalQ54GJ2.
SMRQ54GJ2. Positions 509-803.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0230084.

Proteomic databases

PRIDEQ54GJ2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230084; DDB0230084; DDB_G0290121.
GeneID8627491.
KEGGddi:DDB_G0290121.

Organism-specific databases

dictyBaseDDB_G0290121. purD.

Phylogenomic databases

eggNOGCOG0151.
OMAGMLFTGF.
PhylomeDBQ54GJ2.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.
UPA00074; UER00129.

Family and domain databases

Gene3D3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54GJ2.

Entry information

Entry namePUR2_DICDI
AccessionPrimary (citable) accession number: Q54GJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase