Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional purine biosynthetic protein purD

Gene

purD

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.
ATP + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Phosphoribosylformylglycinamidine synthase (purL)
  2. Bifunctional purine biosynthetic protein purD (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase (purF)
  2. Bifunctional purine biosynthetic protein purD (purD)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi313ManganeseBy similarity1
Metal bindingi315ManganeseBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi139 – 200ATPBy similarityAdd BLAST62

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase, Multifunctional enzyme
Biological processPurine biosynthesis
LigandATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00125
UPA00074; UER00129

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthetic protein purD
Including the following 2 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:purD
ORF Names:DDB_G0290121
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290121 purD

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003283261 – 815Bifunctional purine biosynthetic protein purDAdd BLAST815

Proteomic databases

PaxDbiQ54GJ2
PRIDEiQ54GJ2

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0230084

Structurei

3D structure databases

ProteinModelPortaliQ54GJ2
SMRiQ54GJ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini113 – 343ATP-graspAdd BLAST231

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 452GARSAdd BLAST448
Regioni467 – 808AIRSAdd BLAST342

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.Curated
In the C-terminal section; belongs to the AIR synthase family.Curated

Phylogenomic databases

eggNOGiKOG0237 Eukaryota
COG0150 LUCA
COG0151 LUCA
InParanoidiQ54GJ2
KOiK11788
OMAiLLERHNC
PhylomeDBiQ54GJ2

Family and domain databases

CDDicd02196 PurM, 1 hit
Gene3Di3.30.1330.10, 1 hit
3.30.1490.20, 1 hit
3.90.600.10, 1 hit
3.90.650.10, 1 hit
HAMAPiMF_00741 AIRS, 1 hit
MF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020562 PRibGlycinamide_synth_N
IPR010918 PurM-like_C_dom
IPR036676 PurM-like_C_sf
IPR016188 PurM-like_N
IPR036921 PurM-like_N_sf
IPR004733 PurM_cligase
IPR011054 Rudment_hybrid_motif
PANTHERiPTHR10520 PTHR10520, 1 hit
PfamiView protein in Pfam
PF00586 AIRS, 1 hit
PF02769 AIRS_C, 1 hit
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
SSF55326 SSF55326, 1 hit
SSF56042 SSF56042, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
TIGR00878 purM, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q54GJ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIKNNILVI GSGSREHAIT WKLLESNQVD KVILVPGNAS SSTMERVITV
60 70 80 90 100
ECSKIDAESI SNICKEHNVE YVFVGPEVPL VDGIVDGLKR KGISCFGPTK
110 120 130 140 150
KAAQLEGSKV FCKDFMARNN IPSARYQTFT DYNKAKQYIE SLNYKIVLKA
160 170 180 190 200
SGCAAGKGVL IPNNKEEELE GLKRIMVDKE FGSAGDEIVI EEFLDGEECS
210 220 230 240 250
LMCFSDGYSL VVMPPAQDHK RIFDGDKGAN TGGMGAYAPA PFIVDCNNNA
260 270 280 290 300
TTDKSKSKSS FGTIIDRCVE TILKPTINGM RKEGKPFVGV LFAGLMVSSS
310 320 330 340 350
SSTTNDKVIN VLEFNCRMGD PETQVVLPLL ETDLFEIVLA CIEGRLDGLD
360 370 380 390 400
VKWSNKFAVT VVAASKGYPD SYPKGLKING LLENKNTNDN IIFQAGTTVN
410 420 430 440 450
GSNDIVTNGG RVLSCTSVSE SLEDAIKNSY KLIETISFEG MQYRKDIGQK
460 470 480 490 500
ALNHLERKKQ QQANSKQSVS YSESGVDIER GDAVVDNIGP LAKATTRLGC
510 520 530 540 550
VSDLGGFGAL FDTKAAGFRD PILVSGTDGV GTKLKIAQEL GIHDSIGIDL
560 570 580 590 600
VAMCVNDVVV QGAEPLFFLD YFATGRIHVD VATQVVSGIA RGCKESGCAL
610 620 630 640 650
IGGETAEMPG MYKDGEYDLA GFSVGAVERD QMLPSNIQEG NILLGLASSG
660 670 680 690 700
VHSNGYSLVR YLIETKSGGL TYNSIAPFDS SKTLGQVLLT PTKLYVLSCL
710 720 730 740 750
AAIKSGGVNG LAHITGGGIT ENLPRVIPDG LDCEVELGSW EILPIFKYLV
760 770 780 790 800
ELGNMETEEL LKTFNSGIGM ILIVSPDKVD SITKSLESNN EKVYKIGKII
810
NSKTSKQSKS KVIYK
Length:815
Mass (Da):87,706
Last modified:May 24, 2005 - v1
Checksum:iC24A183386A06FC6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000153 Genomic DNA Translation: EAL62377.1
RefSeqiXP_635881.1, XM_630789.1

Genome annotation databases

EnsemblProtistsiEAL62377; EAL62377; DDB_G0290121
GeneIDi8627491
KEGGiddi:DDB_G0290121

Similar proteinsi

Entry informationi

Entry nameiPUR2_DICDI
AccessioniPrimary (citable) accession number: Q54GJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health