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Q54GJ2

- PUR2_DICDI

UniProt

Q54GJ2 - PUR2_DICDI

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Protein

Bifunctional purine biosynthetic protein purD

Gene
purD, DDB_G0290121
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation
ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi313 – 3131Manganese By similarity
Metal bindingi315 – 3151Manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi139 – 20062ATP By similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. phosphoribosylamine-glycine ligase activity Source: dictyBase
  4. phosphoribosylformylglycinamidine cyclo-ligase activity Source: dictyBase

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
  3. purine nucleobase metabolic process Source: dictyBase
  4. purine nucleotide biosynthetic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
UPA00074; UER00129.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional purine biosynthetic protein purD
Including the following 2 domains:
Phosphoribosylamine--glycine ligase (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Phosphoribosylformylglycinamidine cyclo-ligase (EC:6.3.3.1)
Alternative name(s):
AIR synthase
Short name:
AIRS
Phosphoribosyl-aminoimidazole synthetase
Gene namesi
Name:purD
ORF Names:DDB_G0290121
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290121. purD.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 815815Bifunctional purine biosynthetic protein purDUniRule annotationPRO_0000328326Add
BLAST

Proteomic databases

PRIDEiQ54GJ2.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0230084.

Structurei

3D structure databases

ProteinModelPortaliQ54GJ2.
SMRiQ54GJ2. Positions 509-803.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini113 – 343231ATP-graspAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 452448GARSUniRule annotationAdd
BLAST
Regioni467 – 808342AIRSUniRule annotationAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the GARS family.
In the C-terminal section; belongs to the AIR synthase family.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG0151.
KOiK11788.
OMAiGMLFTGF.
PhylomeDBiQ54GJ2.

Family and domain databases

Gene3Di3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54GJ2-1 [UniParc]FASTAAdd to Basket

« Hide

MTIKNNILVI GSGSREHAIT WKLLESNQVD KVILVPGNAS SSTMERVITV    50
ECSKIDAESI SNICKEHNVE YVFVGPEVPL VDGIVDGLKR KGISCFGPTK 100
KAAQLEGSKV FCKDFMARNN IPSARYQTFT DYNKAKQYIE SLNYKIVLKA 150
SGCAAGKGVL IPNNKEEELE GLKRIMVDKE FGSAGDEIVI EEFLDGEECS 200
LMCFSDGYSL VVMPPAQDHK RIFDGDKGAN TGGMGAYAPA PFIVDCNNNA 250
TTDKSKSKSS FGTIIDRCVE TILKPTINGM RKEGKPFVGV LFAGLMVSSS 300
SSTTNDKVIN VLEFNCRMGD PETQVVLPLL ETDLFEIVLA CIEGRLDGLD 350
VKWSNKFAVT VVAASKGYPD SYPKGLKING LLENKNTNDN IIFQAGTTVN 400
GSNDIVTNGG RVLSCTSVSE SLEDAIKNSY KLIETISFEG MQYRKDIGQK 450
ALNHLERKKQ QQANSKQSVS YSESGVDIER GDAVVDNIGP LAKATTRLGC 500
VSDLGGFGAL FDTKAAGFRD PILVSGTDGV GTKLKIAQEL GIHDSIGIDL 550
VAMCVNDVVV QGAEPLFFLD YFATGRIHVD VATQVVSGIA RGCKESGCAL 600
IGGETAEMPG MYKDGEYDLA GFSVGAVERD QMLPSNIQEG NILLGLASSG 650
VHSNGYSLVR YLIETKSGGL TYNSIAPFDS SKTLGQVLLT PTKLYVLSCL 700
AAIKSGGVNG LAHITGGGIT ENLPRVIPDG LDCEVELGSW EILPIFKYLV 750
ELGNMETEEL LKTFNSGIGM ILIVSPDKVD SITKSLESNN EKVYKIGKII 800
NSKTSKQSKS KVIYK 815
Length:815
Mass (Da):87,706
Last modified:May 24, 2005 - v1
Checksum:iC24A183386A06FC6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000153 Genomic DNA. Translation: EAL62377.1.
RefSeqiXP_635881.1. XM_630789.1.

Genome annotation databases

EnsemblProtistsiDDB0230084; DDB0230084; DDB_G0290121.
GeneIDi8627491.
KEGGiddi:DDB_G0290121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000153 Genomic DNA. Translation: EAL62377.1 .
RefSeqi XP_635881.1. XM_630789.1.

3D structure databases

ProteinModelPortali Q54GJ2.
SMRi Q54GJ2. Positions 509-803.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0230084.

Proteomic databases

PRIDEi Q54GJ2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0230084 ; DDB0230084 ; DDB_G0290121 .
GeneIDi 8627491.
KEGGi ddi:DDB_G0290121.

Organism-specific databases

dictyBasei DDB_G0290121. purD.

Phylogenomic databases

eggNOGi COG0151.
KOi K11788.
OMAi GMLFTGF.
PhylomeDBi Q54GJ2.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
UPA00074 ; UER00129 .

Miscellaneous databases

PROi Q54GJ2.

Family and domain databases

Gene3Di 3.30.1330.10. 1 hit.
3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
3.90.650.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR010918. AIR_synth_C_dom.
IPR000728. AIR_synth_N_dom.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020562. PRibGlycinamide_synth_N.
IPR004733. PurM_cligase.
IPR016188. PurM_N-like.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
SSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
TIGR00878. purM. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Ura S., Insall R.H.
    Submitted (JUL-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 515-533 AND 667-682, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: AX2.

Entry informationi

Entry nameiPUR2_DICDI
AccessioniPrimary (citable) accession number: Q54GJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi