Reviewed,
UniProtKB/Swiss-Prot Q54GJ2 (PUR2_DICDI)
Last modified
February 9, 2010.
Version 40.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Bifunctional purine biosynthetic protein purD Including the following 2 domains: 1- Recommended name: Phosphoribosylamine--glycine ligase EC=6.3.4.13 Alternative name(s): Glycinamide ribonucleotide synthetase Short name=GARS Phosphoribosylglycinamide synthetase 2- Recommended name: Phosphoribosylformylglycinamidine cyclo-ligase EC=6.3.3.1 Alternative name(s): Phosphoribosyl-aminoimidazole synthetase AIR synthase Short name=AIRS | ||||
| Gene names |
| ||||
| Organism | Dictyostelium discoideum (Slime mold) [Complete proteome] | ||||
| Taxonomic identifier | 44689 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium |
Protein attributes
| Sequence length | 815 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. |
| Pathway | |
| Sequence similarities | In the N-terminal section; belongs to the GARS family. In the C-terminal section; belongs to the AIR synthase family. Contains 1 ATP-grasp domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Purine biosynthesis |
| Ligand | ATP-binding Manganese Metal-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Complete proteome Direct protein sequencing Multifunctional enzyme |
| Gene Ontology (GO) | |
| Biological process | 'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro purine base biosynthetic processInferred from electronic annotation. Source: InterPro |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: InterPro |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activityInferred from electronic annotation. Source: EC phosphoribosylformylglycinamidine cyclo-ligase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 815 | 815 | Bifunctional purine biosynthetic protein purD | PRO_0000328326 | |||||
Regions | |||||||||
| Domain | 113 – 343 | 231 | ATP-grasp | ||||||
| Nucleotide binding | 139 – 200 | 62 | ATP By similarity | ||||||
| Region | 5 – 452 | 448 | GARS | ||||||
| Region | 467 – 808 | 342 | AIRS | ||||||
Sites | |||||||||
| Metal binding | 313 | 1 | Manganese By similarity | ||||||
| Metal binding | 315 | 1 | Manganese By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. Kuspa A.Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4. |
| [2] | Bienvenut W.V., Ura S., Insall R.H. Submitted (JUL-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 515-533 AND 667-682, MASS SPECTROMETRY. Strain: AX2. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AAFI02000153 Genomic DNA. Translation: EAL62377.1. |
| RefSeq | XP_635881.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1CLI based on UniProtKB P08178. |
| SMR | Q54GJ2. Positions 2-455, 468-804. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q54GJ2. |
Genome annotation databases | |
| GeneID | 8627491. |
| KEGG | ddi:DDB_0230084. |
Organism-specific databases | |
| dictyBase | DDB_G0290121. purD. |
Phylogenomic databases | |
| eggNOG | KOG0237. |
| HOGENOM | HBG328156. |
| OMA | GETSEMP. |
| PhylomeDB | Q54GJ2. |
Enzyme and pathway databases | |
| BRENDA | 6.3.3.1. 424. 6.3.4.13. 424. |
Family and domain databases | |
| InterPro | IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020562. PRibGlycinamide_synth_N-dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. IPR011054. Rudment_hybrid_motif. [Graphical view] |
| Gene3D | G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| Pfam | PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00877. purD. 1 hit. TIGR00878. purM. 1 hit. |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS00184. GARS. False negative. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PUR2_DICDI | ||||||||
| Accession | Primary (citable) accession number: Q54GJ2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| Dictyostelium discoideum Dictyostelium discoideum: entries, gene names and cross-references to dictyBase |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


