Q54GH4 (MIOX_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inositol oxygenase
EC=1.13.99.1

Alternative name(s):
Myo-inositol oxygenase
Short name=MI oxygenase

Gene names

Name:	miox
ORF Names:	DDB_G0290161

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Myo-inositol + O2 = D-glucuronate + H2O.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into D-glucuronate; D-glucuronate from myo-inositol: step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the myo-inositol oxygenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inositol oxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Inositol oxygenase		PRO_0000328413
Regions
	Region	88 – 90	3	Substrate binding By similarity
	Region	149 – 150	2	Substrate binding By similarity
	Region	227 – 228	2	Substrate binding By similarity
Sites
	Metal binding	101	1	Iron 1 By similarity
	Metal binding	128	1	Iron 1 By similarity
	Metal binding	129	1	Iron 1 By similarity
	Metal binding	129	1	Iron 2 By similarity
	Metal binding	201	1	Iron 2 By similarity
	Metal binding	227	1	Iron 2 By similarity
	Metal binding	260	1	Iron 1 By similarity
	Binding site	33	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54GH4 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 4F4D1E483BECFF8E	FASTA	292	34,801
	10 20 30 40 50 60 MIETRTTTST SEIKHDNSLK TGFEITKEVE EFRNYENSED RVSEAYRNSH TYQTYDYATE 70 80 90 100 110 120 KKKQYSQLDT SIKMGLWEAA ELLNTIIDES DPDSNIPQIN HCLQTAEAIR KVYPDSKYDW 130 140 150 160 170 180 FHLTGFIHDL GKVLLSKKFK EQPQWATVGD TFPLGCKFDE SNIFYEFFKM NPDYNDSKYN 190 200 210 220 230 240 SECGIYKKNI GLENVTMSWG HDEYFYLVCV GNKCLLPKES LYMIRFHSFY PWHRHNKYTH 250 260 270 280 290 LTNEEDEKML NWVKEFNKFD LYSKDSEPVD VESLKPYYQS LISKYFPNEL HW

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000158 Genomic DNA. Translation: EAL62352.1.
RefSeq	XP_635854.1. XM_630762.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54GH4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0235367; DDB0235367; DDB_G0290161.
GeneID	8627510.
GenomeReviews	Gene locus miox in contig CM000154_GR.
KEGG	ddi:DDB_G0290161.
Organism-specific databases
dictyBase	DDB_G0290161. miox.
Phylogenomic databases
eggNOG	KOG1573.
GeneTree	EPrGT00050000006088.
HOGENOM	HBG332059.
OMA	VMEAVDM.
PhylomeDB	Q54GH4.
ProtClustDB	CLSZ2429697.
Family and domain databases
InterPro	IPR007828. Inositol_oxygenase. [Graphical view]
KO	K00469.
PANTHER	PTHR12588. DUF706. 1 hit.
Pfam	PF05153. DUF706. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIOX_DICDI

Accession

Primary (citable) accession number: Q54GH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)