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Protein

Probable malate dehydrogenase 1

Gene

mdhA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei149 – 1491SubstrateBy similarity
Binding sitei155 – 1551SubstrateBy similarity
Binding sitei162 – 1621NADBy similarity
Binding sitei169 – 1691NADBy similarity
Binding sitei188 – 1881SubstrateBy similarity
Binding sitei219 – 2191SubstrateBy similarity
Active sitei244 – 2441Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi68 – 747NADBy similarity
Nucleotide bindingi186 – 1883NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Probable malate dehydrogenase 1 (EC:1.1.1.37)
Gene namesi
Name:mdhA
ORF Names:DDB_G0290207
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290207. mdhA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 391391Probable malate dehydrogenase 1PRO_0000312365Add
BLAST

Proteomic databases

PRIDEiQ54GE6.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0230186.

Structurei

3D structure databases

ProteinModelPortaliQ54GE6.
SMRiQ54GE6. Positions 60-391.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
InParanoidiQ54GE6.
KOiK00025.
OMAiHINVAIL.
PhylomeDBiQ54GE6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54GE6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQAILRINN ISSQLTGKCP FKAHGGGAGK CPYKLGGGQI LDQSKAWSLV
60 70 80 90 100
NVTANVDQVT PIKVLVTGAA GQIAYSLMFM IASGQMFGPH QPVILHLLDI
110 120 130 140 150
PKMADALKGV VMELQDCSYP LLQSVVATTD IQTAFLHINV AILVGAFPRG
160 170 180 190 200
PGMQRKDLLK VNVSIFKEQG EALNKYASRG VKVLVVGNPA NTNALTALMK
210 220 230 240 250
ASDLPTSNFS ALTRLDQNRA QSMISEKVGT NVDNVHNVII WGNHSQTQVP
260 270 280 290 300
DVNHGYILNY PNRGLIEPIP SSVNDDKWLN EQFIPLVQNR GATVIAARKL
310 320 330 340 350
SSAASAANAI VGHVRDWLLG TKDGEHVSMA VYSDGSYNVP KGLIFSFPVT
360 370 380 390
CHNGQWTIVQ GLKINSSTQQ KIDATIKELQ EEKETAMSFL N
Length:391
Mass (Da):42,265
Last modified:May 24, 2005 - v1
Checksum:iE9433362AD830967
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000161 Genomic DNA. Translation: EAL62325.1.
RefSeqiXP_635832.1. XM_630740.1.

Genome annotation databases

EnsemblProtistsiDDB0230186; DDB0230186; DDB_G0290207.
GeneIDi8627540.
KEGGiddi:DDB_G0290207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000161 Genomic DNA. Translation: EAL62325.1.
RefSeqiXP_635832.1. XM_630740.1.

3D structure databases

ProteinModelPortaliQ54GE6.
SMRiQ54GE6. Positions 60-391.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0230186.

Proteomic databases

PRIDEiQ54GE6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0230186; DDB0230186; DDB_G0290207.
GeneIDi8627540.
KEGGiddi:DDB_G0290207.

Organism-specific databases

dictyBaseiDDB_G0290207. mdhA.

Phylogenomic databases

eggNOGiCOG0039.
InParanoidiQ54GE6.
KOiK00025.
OMAiHINVAIL.
PhylomeDBiQ54GE6.

Miscellaneous databases

PROiQ54GE6.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Veltman D.M., Insall R.H.
    Submitted (JAN-2010) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 35-45 AND 201-214, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiMDHA_DICDI
AccessioniPrimary (citable) accession number: Q54GE6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 24, 2005
Last modified: April 29, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.