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Q54GE6

- MDHA_DICDI

UniProt

Q54GE6 - MDHA_DICDI

Protein

Probable malate dehydrogenase 1

Gene

mdhA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei149 – 1491SubstrateBy similarity
    Binding sitei155 – 1551SubstrateBy similarity
    Binding sitei162 – 1621NADBy similarity
    Binding sitei169 – 1691NADBy similarity
    Binding sitei188 – 1881SubstrateBy similarity
    Binding sitei219 – 2191SubstrateBy similarity
    Active sitei244 – 2441Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi68 – 747NADBy similarity
    Nucleotide bindingi186 – 1883NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable malate dehydrogenase 1 (EC:1.1.1.37)
    Gene namesi
    Name:mdhA
    ORF Names:DDB_G0290207
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0290207. mdhA.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391Probable malate dehydrogenase 1PRO_0000312365Add
    BLAST

    Proteomic databases

    PRIDEiQ54GE6.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0230186.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54GE6.
    SMRiQ54GE6. Positions 60-391.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

    Phylogenomic databases

    eggNOGiCOG0039.
    KOiK00025.
    OMAiWCPWLLR.
    PhylomeDBiQ54GE6.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_01517. Malate_dehydrog_2.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54GE6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQAILRINN ISSQLTGKCP FKAHGGGAGK CPYKLGGGQI LDQSKAWSLV    50
    NVTANVDQVT PIKVLVTGAA GQIAYSLMFM IASGQMFGPH QPVILHLLDI 100
    PKMADALKGV VMELQDCSYP LLQSVVATTD IQTAFLHINV AILVGAFPRG 150
    PGMQRKDLLK VNVSIFKEQG EALNKYASRG VKVLVVGNPA NTNALTALMK 200
    ASDLPTSNFS ALTRLDQNRA QSMISEKVGT NVDNVHNVII WGNHSQTQVP 250
    DVNHGYILNY PNRGLIEPIP SSVNDDKWLN EQFIPLVQNR GATVIAARKL 300
    SSAASAANAI VGHVRDWLLG TKDGEHVSMA VYSDGSYNVP KGLIFSFPVT 350
    CHNGQWTIVQ GLKINSSTQQ KIDATIKELQ EEKETAMSFL N 391
    Length:391
    Mass (Da):42,265
    Last modified:May 24, 2005 - v1
    Checksum:iE9433362AD830967
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000161 Genomic DNA. Translation: EAL62325.1.
    RefSeqiXP_635832.1. XM_630740.1.

    Genome annotation databases

    EnsemblProtistsiDDB0230186; DDB0230186; DDB_G0290207.
    GeneIDi8627540.
    KEGGiddi:DDB_G0290207.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000161 Genomic DNA. Translation: EAL62325.1 .
    RefSeqi XP_635832.1. XM_630740.1.

    3D structure databases

    ProteinModelPortali Q54GE6.
    SMRi Q54GE6. Positions 60-391.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0230186.

    Proteomic databases

    PRIDEi Q54GE6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0230186 ; DDB0230186 ; DDB_G0290207 .
    GeneIDi 8627540.
    KEGGi ddi:DDB_G0290207.

    Organism-specific databases

    dictyBasei DDB_G0290207. mdhA.

    Phylogenomic databases

    eggNOGi COG0039.
    KOi K00025.
    OMAi WCPWLLR.
    PhylomeDBi Q54GE6.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_01517. Malate_dehydrog_2.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR011274. Malate_DH_NAD-dep_euk.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    TIGR01758. MDH_euk_cyt. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. Bienvenut W.V., Veltman D.M., Insall R.H.
      Submitted (JAN-2010) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 35-45 AND 201-214, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiMDHA_DICDI
    AccessioniPrimary (citable) accession number: Q54GE6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 4, 2007
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3