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Q54GE6 (MDHA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable malate dehydrogenase 1

EC=1.1.1.37
Gene names
Name:mdhA
ORF Names:DDB_G0290207
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_01517

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_01517

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01517

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391Probable malate dehydrogenase 1 HAMAP-Rule MF_01517
PRO_0000312365

Regions

Nucleotide binding68 – 747NAD By similarity
Nucleotide binding186 – 1883NAD By similarity

Sites

Active site2441Proton acceptor By similarity
Binding site1491Substrate By similarity
Binding site1551Substrate By similarity
Binding site1621NAD By similarity
Binding site1691NAD By similarity
Binding site1881Substrate By similarity
Binding site2191Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54GE6 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: E9433362AD830967

FASTA39142,265
        10         20         30         40         50         60 
MEQAILRINN ISSQLTGKCP FKAHGGGAGK CPYKLGGGQI LDQSKAWSLV NVTANVDQVT 

        70         80         90        100        110        120 
PIKVLVTGAA GQIAYSLMFM IASGQMFGPH QPVILHLLDI PKMADALKGV VMELQDCSYP 

       130        140        150        160        170        180 
LLQSVVATTD IQTAFLHINV AILVGAFPRG PGMQRKDLLK VNVSIFKEQG EALNKYASRG 

       190        200        210        220        230        240 
VKVLVVGNPA NTNALTALMK ASDLPTSNFS ALTRLDQNRA QSMISEKVGT NVDNVHNVII 

       250        260        270        280        290        300 
WGNHSQTQVP DVNHGYILNY PNRGLIEPIP SSVNDDKWLN EQFIPLVQNR GATVIAARKL 

       310        320        330        340        350        360 
SSAASAANAI VGHVRDWLLG TKDGEHVSMA VYSDGSYNVP KGLIFSFPVT CHNGQWTIVQ 

       370        380        390 
GLKINSSTQQ KIDATIKELQ EEKETAMSFL N 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]Bienvenut W.V., Veltman D.M., Insall R.H.
Submitted (JAN-2010) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 35-45 AND 201-214, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000161 Genomic DNA. Translation: EAL62325.1.
RefSeqXP_635832.1. XM_630740.1.

3D structure databases

ProteinModelPortalQ54GE6.
SMRQ54GE6. Positions 60-391.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0230186.

Proteomic databases

PRIDEQ54GE6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230186; DDB0230186; DDB_G0290207.
GeneID8627540.
KEGGddi:DDB_G0290207.

Organism-specific databases

dictyBaseDDB_G0290207. mdhA.

Phylogenomic databases

eggNOGCOG0039.
OMAWCPWLLR.
PhylomeDBQ54GE6.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_01517. Malate_dehydrog_2.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHA_DICDI
AccessionPrimary (citable) accession number: Q54GE6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase