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Protein

Beta-galactosidase 1

Gene

glb1

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei200 – 2001Proton donorSequence Analysis
Active sitei288 – 2881NucleophileSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_285350. Keratan sulfate degradation.
REACT_307367. HS-GAG degradation.
REACT_349535. Glycosphingolipid metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase 1 (EC:3.2.1.23)
Alternative name(s):
Acid beta-galactosidase 1
Short name:
Lactase 1
Gene namesi
Name:glb1
ORF Names:DDB_G0290217
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290217. glb1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence AnalysisAdd
BLAST
Chaini19 – 671653Beta-galactosidase 1PRO_0000328062Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi321 – 3211N-linked (GlcNAc...)Sequence Analysis
Glycosylationi391 – 3911N-linked (GlcNAc...)Sequence Analysis
Glycosylationi400 – 4001N-linked (GlcNAc...)Sequence Analysis
Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi509 – 5091N-linked (GlcNAc...)Sequence Analysis
Glycosylationi564 – 5641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi595 – 5951N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ54GE1.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0266380.

Structurei

3D structure databases

ProteinModelPortaliQ54GE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1874.
InParanoidiQ54GE1.
KOiK12309.
OMAiHWAEPFP.
PhylomeDBiQ54GE1.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54GE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIVLIFFL LFINLNYCLK IKEQPKVGGI DSEQPTNSFY IENGNFLMNN
60 70 80 90 100
QGNGGSLNQY QIISGSFHYF RCLPELWVDR LTKMKACGLN TIQTYIPWNV
110 120 130 140 150
HQPNGFNTEL VATNDLIEFL RQAQQIGLNV ILRPGPYSCA EWELGGFPYW
160 170 180 190 200
ILEQQPIALR SSDSVFISAV IAYWSRLLPL LEPLLFTNGG PIIMVQVENE
210 220 230 240 250
YGSYGEDKSY LETLLTLLQK YLGQGDGNGS GVLFHSTDGP SAQMLFGSKL
260 270 280 290 300
EGVYQTVDFG PMPIEQIQDN FKIQQTFASK PTPPMNSEYY TGWITNWGDA
310 320 330 340 350
SAARTDASVV AQGLDDILSL NASVNMYMFF GGSNAGFMNG ANSNSPTTNF
360 370 380 390 400
EITIQSYDYD SPLSESGDTT EKYFAIKNVI EKYIKPTTNS NSTLPPIPSN
410 420 430 440 450
STKVAYGTIQ ITQATSLFNN LANLVNSNQQ QLQTGAPIPM EQLQQSTGFV
460 470 480 490 500
LYETTMNIAQ SSQLSITELH DRATIFINDE AIEDTQTIGQ AVFLQRPFNS
510 520 530 540 550
SIEITYPSNV TDDGNFNLRI LLENQGRVNF GPYLNDRKGL LSGGVLSGQQ
560 570 580 590 600
YLGPWNNYPL PLTNKTLSNI NNWEQIKDYT LSNTPTFYQA TLSLNSTNDI
610 620 630 640 650
GDTFLSFTGL GKGQLFVNGY NVGRYWNVGP QRTIYISSVL LHQGDNEIIL
660 670
FETLLTQPIF EIQFLNQPYF D
Length:671
Mass (Da):74,979
Last modified:May 24, 2005 - v1
Checksum:i27F7DD24EDA31925
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000161 Genomic DNA. Translation: EAL62330.1.
RefSeqiXP_635837.1. XM_630745.1.

Genome annotation databases

EnsemblProtistsiDDB0266380; DDB0266380; DDB_G0290217.
GeneIDi8627545.
KEGGiddi:DDB_G0290217.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000161 Genomic DNA. Translation: EAL62330.1.
RefSeqiXP_635837.1. XM_630745.1.

3D structure databases

ProteinModelPortaliQ54GE1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0266380.

Proteomic databases

PRIDEiQ54GE1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0266380; DDB0266380; DDB_G0290217.
GeneIDi8627545.
KEGGiddi:DDB_G0290217.

Organism-specific databases

dictyBaseiDDB_G0290217. glb1.

Phylogenomic databases

eggNOGiCOG1874.
InParanoidiQ54GE1.
KOiK12309.
OMAiHWAEPFP.
PhylomeDBiQ54GE1.

Enzyme and pathway databases

ReactomeiREACT_285350. Keratan sulfate degradation.
REACT_307367. HS-GAG degradation.
REACT_349535. Glycosphingolipid metabolism.

Miscellaneous databases

PROiQ54GE1.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiBGAL1_DICDI
AccessioniPrimary (citable) accession number: Q54GE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 22, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.