Reviewed, UniProtKB/Swiss-Prot Q54GC1 (DGAT2_DICDI)

Names and origin

Protein names

Recommended name:
    Diacylglycerol O-acyltransferase 2
    EC=2.3.1.20
Alternative name(s):
    Diglyceride acyltransferase 2

Gene names

Name:	dgat2
ORF Names:	DDB_G0290279

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis By similarity.
Catalytic activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the diacylglycerol acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Biological process	Glycerol metabolism Lipid metabolism Lipid synthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW lipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	diacylglycerol O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Diacylglycerol O-acyltransferase 2		PRO_0000328257
Regions
	Transmembrane	15 – 35	21	Potential
	Transmembrane	37 – 57	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54GC1-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 1F0A08B0921FE01E	FASTA	330	37,330
	10 20 30 40 50 60 MVRFVPWNVP LYRRLETMAV AIYAMVLPVC LIMAFNLIVI PLFWGIAIPY LVWMFYFDTK 70 80 90 100 110 120 HESGGRRVSL VRNSILWRYF RDYFPISLII NSNYDPKKNY IFAYHPHGII SIGAFCNFAT 130 140 150 160 170 180 NANNIDEKLP GLKVHLLTLE SNFKIPFLRD VLMSFGMSSV SKKSCENILN SGAGESICLV 190 200 210 220 230 240 VGGAEESLDA RPGLNEITLK KRKGFIKLAL VNGASLVPVY SFGENDIYDQ VPNPRGSLVR 250 260 270 280 290 300 KIQTKIKDLT GIAPPLFMGR GIFNYDFGLL PVRHKIVTVV GEPIDIPKIK SPTDQVIEHY 310 320 330 HQIYVEALQN LFDKHKNSCA DKETGNLKIN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000162 Genomic DNA. Translation: EAL62266.1.
RefSeq	XP_635762.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3387386.
GenomeReviews	Gene locus dgat2 in contig CM000154_GR.
KEGG	ddi:DDB_0233097.
Organism-specific databases
dictyBase	DDB_G0290279. dgat2.
Phylogenomic databases
OMA	GAGESIC.
Enzyme and pathway databases
BRENDA	2.3.1.20. 424.
Family and domain databases
InterPro	IPR007130. DAGAT. [Graphical view]
PANTHER	PTHR12317. DAGAT. 1 hit.
Pfam	PF03982. DAGAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGAT2_DICDI

Accession

Primary (citable) accession number: Q54GC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)