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Protein

Probable polyketide synthase 37

Gene

stlB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Does make the polyketide skeleton of dif-1.1 Publication

Miscellaneous

Encoded by one of the numerous copies of polyketide synthase genes localized in chromosome 5.
In reference to their hybrid nature and to their discovery in D.discoideum, authors term these type I FAS-type III PKS fusion enzymes 'steely'.1 Publication

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Cofactori

pantetheine 4'-phosphateBy similarityNote: Binds 1 phosphopantetheine covalently.By similarity

Pathwayi: flavonoid biosynthesis

This protein is involved in the pathway flavonoid biosynthesis, which is part of Secondary metabolite biosynthesis.
View all proteins of this organism that are known to be involved in the pathway flavonoid biosynthesis and in Secondary metabolite biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei198For beta-ketoacyl synthase activityPROSITE-ProRule annotation1
Active sitei718For acyl/malonyl transferase activityPROSITE-ProRule annotation1
Active sitei2747PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • DIF-1 biosynthetic process Source: dictyBase
  • flavonoid biosynthetic process Source: UniProtKB-UniPathway
  • negative regulation of gene expression Source: dictyBase
  • polyketide biosynthetic process Source: dictyBase
  • positive regulation of gene expression Source: dictyBase
  • sorocarp morphogenesis Source: dictyBase
  • sorocarp stalk cell differentiation Source: dictyBase

Keywordsi

Molecular functionAcyltransferase, Multifunctional enzyme, Transferase

Enzyme and pathway databases

ReactomeiR-DDI-163765 ChREBP activates metabolic gene expression
R-DDI-199220 Vitamin B5 (pantothenate) metabolism
R-DDI-75105 Fatty acyl-CoA biosynthesis
UniPathwayiUPA00154

Names & Taxonomyi

Protein namesi
Recommended name:
Probable polyketide synthase 37
Short name:
dipks37
Including the following 2 domains:
Polyketide synthase stlB (EC:2.3.1.-)
Chalcone synthase stlB (EC:2.3.1.74)
Alternative name(s):
Steely2
Gene namesi
Name:stlB
Synonyms:pks37
ORF Names:DDB_G0290853
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290853 stlB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1839 – 1859HelicalSequence analysisAdd BLAST21
Transmembranei1869 – 1889HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: dictyBase

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Developed to the slug stage, in which dif-1 accumulation is maximal, though the Steely2- mutant slugs were thin and tended to break up. However, two independent Steely2 mutant strains both failed to accumulate any detectable dif-1 at this or any other stage of development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003779021 – 2968Probable polyketide synthase 37Add BLAST2968

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2458O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation1

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PaxDbiQ54FI3
PRIDEiQ54FI3

Expressioni

Developmental stagei

Expressed during development. Coordinately expressed with dmtA.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0234163

Structurei

3D structure databases

ProteinModelPortaliQ54FI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2421 – 2498CarrierPROSITE-ProRule annotationAdd BLAST78

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 232Beta-ketoacyl synthaseAdd BLAST54
Regioni708 – 742Acyl/malonyl transferaseAdd BLAST35
Regioni2707 – 2968Chalcone synthaseAdd BLAST262

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 28Asn-richAdd BLAST27
Compositional biasi465 – 469Poly-Thr5
Compositional biasi475 – 479Poly-Ser5
Compositional biasi585 – 588Poly-Asn4
Compositional biasi597 – 608Poly-AsnAdd BLAST12
Compositional biasi609 – 617Poly-Ser9
Compositional biasi618 – 621Poly-Asn4
Compositional biasi674 – 677Poly-Gly4
Compositional biasi978 – 982Poly-Gln5
Compositional biasi1076 – 1082Poly-Thr7
Compositional biasi1170 – 1180Poly-ThrAdd BLAST11
Compositional biasi1346 – 1355Poly-Ser10
Compositional biasi1524 – 1528Poly-Gly5
Compositional biasi1535 – 1543Poly-Ser9
Compositional biasi1683 – 1686Poly-Ser4
Compositional biasi1821 – 1826Poly-Ser6
Compositional biasi2376 – 2381Poly-Gly6
Compositional biasi2567 – 2571Poly-Ser5

Domaini

Modular protein possessing six classical catalytic domains and a type III polyketide synthase domain. May facilitate covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1202 Eukaryota
COG3321 LUCA
COG3424 LUCA
InParanoidiQ54FI3
PhylomeDBiQ54FI3

Family and domain databases

Gene3Di1.10.1200.10, 1 hit
3.40.366.10, 1 hit
3.40.47.10, 3 hits
InterProiView protein in InterPro
IPR001227 Ac_transferase_dom_sf
IPR036736 ACP-like_sf
IPR014043 Acyl_transferase
IPR016035 Acyl_Trfase/lysoPLipase
IPR013154 ADH_N
IPR012328 Chalcone/stilbene_synth_C
IPR001099 Chalcone/stilbene_synthase_N
IPR011032 GroES-like_sf
IPR032821 KAsynt_C_assoc
IPR018201 Ketoacyl_synth_AS
IPR014031 Ketoacyl_synth_C
IPR014030 Ketoacyl_synth_N
IPR036291 NAD(P)-bd_dom_sf
IPR020801 PKS_acyl_transferase
IPR020841 PKS_Beta-ketoAc_synthase_dom
IPR020807 PKS_dehydratase
IPR020843 PKS_ER
IPR013968 PKS_KR
IPR020806 PKS_PP-bd
IPR009081 PP-bd_ACP
IPR016039 Thiolase-like
PfamiView protein in Pfam
PF00698 Acyl_transf_1, 1 hit
PF08240 ADH_N, 1 hit
PF02797 Chal_sti_synt_C, 1 hit
PF00195 Chal_sti_synt_N, 1 hit
PF16197 KAsynt_C_assoc, 1 hit
PF00109 ketoacyl-synt, 1 hit
PF02801 Ketoacyl-synt_C, 1 hit
PF08659 KR, 1 hit
PF00550 PP-binding, 1 hit
PF14765 PS-DH, 1 hit
SMARTiView protein in SMART
SM00827 PKS_AT, 1 hit
SM00829 PKS_ER, 1 hit
SM00825 PKS_KS, 1 hit
SM00823 PKS_PP, 1 hit
SUPFAMiSSF47336 SSF47336, 1 hit
SSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 2 hits
SSF52151 SSF52151, 2 hits
SSF53901 SSF53901, 4 hits
PROSITEiView protein in PROSITE
PS00606 B_KETOACYL_SYNTHASE, 1 hit
PS50075 CARRIER, 1 hit

Sequencei

Sequence statusi: Complete.

Q54FI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNNKSINDL SGNSNNNIAN SNINNYNNLI KKEPIAIIGI GCRFPGNVSN
60 70 80 90 100
YSDFVNIIKN GSDCLTKIPD DRWNADIISR KQWKLNNRIG GYLKNIDQFD
110 120 130 140 150
NQFFGISPKE AQHIDPQQRL LLHLAIETLE DGKISLDEIK GKKVGVFIGS
160 170 180 190 200
SSGDYLRGFD SSEINQFTTP GTNSSFLSNR LSYFLDVNGP SMTVNTACSA
210 220 230 240 250
SMVAIHLGLQ SLWNGESELS MVGGVNIISS PLQSLDFGKA GLLNQETDGR
260 270 280 290 300
CYSFDPRASG YVRSEGGGIL LLKPLSAALR DNDEIYSLLL NSANNSNGKT
310 320 330 340 350
PTGITSPRSL CQEKLIQQLL RESSDQFSID DIGYFECHGT GTQMGDLNEI
360 370 380 390 400
TAIGKSIGML KSHDDPLIIG SVKASIGHLE GASGICGVIK SIICLKEKIL
410 420 430 440 450
PQQCKFSSYN PKIPFETLNL KVLTKTQPWN NSKRICGVNS FGVGGSNSSL
460 470 480 490 500
FLSSFDKSTT ITEPTTTTTI ESLPSSSSSF DNLSVSSSIS TNNDNDKVSN
510 520 530 540 550
IVNNRYGSSI DVITLSVTSP DKEDLKIRAN DVLESIKTLD DNFKIRDISN
560 570 580 590 600
LTNIRTSHFS NRVAIIGDSI DSIKLNLQSF IKGENNNNKS IILPLINNGN
610 620 630 640 650
NNNNNNNNSS GSSSSSSNNN NICFIFSGQG QQWNKMIFDL YENNKTFKNE
660 670 680 690 700
MNNFSKQFEM ISGWSIIDKL YNSGGGGNEE LINETWLAQP SIVAVQYSLI
710 720 730 740 750
KLFSKDIGIE GSIVLGHSLG ELMAAYYCGI INDFNDLLKL LYIRSTLQNK
760 770 780 790 800
TNGSGRMHVC LSSKAEIEQL ISQLGFNGRI VICGNNTMKS CTISGDNESM
810 820 830 840 850
NQFTKLISSQ QYGSVVHKEV RTNSAFHSHQ MDIIKDEFFK LFNQYFPTNQ
860 870 880 890 900
ISTNQIYDGK SFYSTCYGKY LTPIECKQLL SSPNYWWKNI RESVLFKESI
910 920 930 940 950
EQILQNHQQS LTFIEITCHP ILNYFLSQLL KSSSKSNTLL LSTLSKNSNS
960 970 980 990 1000
IDQLLILCSK LYVNNLSSIK WNWFYDKQQQ QQSESLVSSN FKLPGRRWKL
1010 1020 1030 1040 1050
EKYWIENCQR QMDRIKPPMF ISLDRKLFSV TPSFEVRLNQ DRFQYLNDHQ
1060 1070 1080 1090 1100
IQDIPLVPFS FYIELVYASI FNSISTTTTN TTASTMFEIE NFTIDSSIII
1110 1120 1130 1140 1150
DQKKSTLIGI NFNSDLTKFE IGSINSIGSG SSSNNNFIEN KWKIHSNGII
1160 1170 1180 1190 1200
KYGTNYLKSN SKSNSFNEST TTTTTTTTTT KCFKSFNSNE FYNEIIKYNY
1210 1220 1230 1240 1250
NYKSTFQCVK EFKQFDKQGT FYYSEIQFKK NDKQVIDQLL SKQLPSDFRC
1260 1270 1280 1290 1300
IHPCLLDAVL QSAIIPATNK TNCSWIPIKI GKLSVNIPSN SYFNFKDQLL
1310 1320 1330 1340 1350
YCLIKPSTST STSPSTYFSS DIQVFDKKNN NLICELTNLE FKGINSSSSS
1360 1370 1380 1390 1400
SSSSSTINSN VEANYESKIE ETNHDEDEDE ELPLVSEYVW CKEELINQSI
1410 1420 1430 1440 1450
KFTDNYQTVI FCSTNLNGND LLDSIITSAL ENGHDENKIF IVSPPPVESD
1460 1470 1480 1490 1500
QYNNRIIINY TNNESDFDAL FAIINSTTSI SGKSGLFSTR FIILPNFNSI
1510 1520 1530 1540 1550
TFSSGNSTPL ITNVNGNGNG KSCGGGGGST NNTISNSSSS ISSIDNGNNE
1560 1570 1580 1590 1600
DEEMVLKSFN DSNLSLFHLQ KSIIKNNIKG RLFLITNGGQ SISSSTPTST
1610 1620 1630 1640 1650
YNDQSYVNLS QYQLIGQIRV FSNEYPIMEC SMIDIQDSTR IDLITDQLNS
1660 1670 1680 1690 1700
TKLSKLEIAF RDNIGYSYKL LKPSIFDNSS LPSSSSEIET TATTKDEEKN
1710 1720 1730 1740 1750
NSINYNNNYY RVELSDNGII SDLKIKQFRQ MKCGVGQVLV RVEMCTLNFR
1760 1770 1780 1790 1800
DILKSLGRDY DPIHLNSMGD EFSGKVIEIG EGVNNLSVGQ YVFGINMSKS
1810 1820 1830 1840 1850
MGSFVCCNSD LVFPIPIPTP SSSSSSNENI DDQEIISKLL NQYCTIPIVF
1860 1870 1880 1890 1900
LTSWYSIVIQ GRLKKGEKIL IHSGCGGVGL ATIQISMMIG AEIHVTVGSN
1910 1920 1930 1940 1950
EKKQYLIKEF GIDEKRIYSS RSLQFYNDLM VNTDGQGVDM VLNSLSGEYL
1960 1970 1980 1990 2000
EKSIQCLSQY GRFIEIGKKD IYSNSSIHLE PFKNNLSFFA VDIAQMTENR
2010 2020 2030 2040 2050
RDYLREIMID QLLPCFKNGS LKPLNQHCFN SPCDLVKAIR FMSSGNHIGK
2060 2070 2080 2090 2100
ILINWSNLNN DKQFINHHSV VHLPIQSFSN RSTYIFTGFG GLTQTLLKYF
2110 2120 2130 2140 2150
STESDLTNVI IVSKNGLDDN SGSGSGNNEK LKLINQLKES GLNVLVEKCD
2160 2170 2180 2190 2200
LSSIKQVYKL FNKIFDNDAS GSDSGDFSDI KGIFHFASLI NDKRILKHNL
2210 2220 2230 2240 2250
ESFNYVYNSK ATSAWNLHQV SLKYNLNLDH FQTIGSVITI LGNIGQSNYT
2260 2270 2280 2290 2300
CANRFVEGLT HLRIGMGLKS SCIHLASIPD VGMASNDNVL NDLNSMGFVP
2310 2320 2330 2340 2350
FQSLNEMNLG FKKLLSSPNP IVVLGEINVD RFIEATPNFR AKDNFIITSL
2360 2370 2380 2390 2400
FNRIDPLLLV NESQDFIINN NINNNGGGGD GSFDDLNQLE DEGQQGFGNG
2410 2420 2430 2440 2450
DGYVDDNIDS VSMLSGTSSI FDNDFYTKSI RGMLCDILEL KDKDLNNTVS
2460 2470 2480 2490 2500
FSDYGLDSLL SSELSNTIQK NFSILIPSLT LVDNSTINST VELIKNKLKN
2510 2520 2530 2540 2550
STTSSISSSV SKKVSFKKNT QPLIIPTTAP ISIIKTQSYI KSEIIESLPI
2560 2570 2580 2590 2600
SSSTTIKPLV FDNLVYSSSS SNNSNSKNEL TSPPPSAKRE SVLPIISEDN
2610 2620 2630 2640 2650
NSDNDSSMAT VIYEISPIAA PYHRYQTDVL KEITQLTPHK EFIDNIYKKS
2660 2670 2680 2690 2700
KIRSRYCFND FSEKSMADIN KLDAGERVAL FREQTYQTVI NAGKTVIERA
2710 2720 2730 2740 2750
GIDPMLISHV VGVTSTGIMA PSFDVVLIDK LGLSINTSRT MINFMGCGAA
2760 2770 2780 2790 2800
VNSMRAATAY AKLKPGTFVL VVAVEASATC MKFNFDSRSD LLSQAIFTDG
2810 2820 2830 2840 2850
CVATLVTCQP KSSLVGKLEI IDDLSYLMPD SRDALNLFIG PTGIDLDLRP
2860 2870 2880 2890 2900
ELPIAINRHI NSAITSWLKK NSLQKSDIEF FATHPGGAKI ISAVHEGLGL
2910 2920 2930 2940 2950
SPEDLSDSYE VMKRYGNMIG VSTYYVLRRI LDKNQTLLQE GSLGYNYGMA
2960
MAFSPGASIE AILFKLIK
Length:2,968
Mass (Da):329,909
Last modified:May 24, 2005 - v1
Checksum:i98AA21F6256A8A57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000171 Genomic DNA Translation: EAL62021.1
RefSeqiXP_635518.1, XM_630426.1

Genome annotation databases

EnsemblProtistsiEAL62021; EAL62021; DDB_G0290853
GeneIDi8627855
KEGGiddi:DDB_G0290853

Entry informationi

Entry nameiPKS37_DICDI
AccessioniPrimary (citable) accession number: Q54FI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 24, 2005
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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