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Q54FI3 (PKS37_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable polyketide synthase 37

Short name=dipks37

Including the following 2 domains:

  1. Polyketide synthase stlB
    EC=2.3.1.-
  2. Chalcone synthase stlB
    EC=2.3.1.74
    Alternative name(s):
    Steely2
Gene names
Name:stlB
Synonyms:pks37
ORF Names:DDB_G0290853
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length2968 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Does make the polyketide skeleton of dif-1. Ref.2

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Cofactor

Binds 1 phosphopantetheine covalently By similarity.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Developmental stage

Expressed during development. Coordinately expressed with dmtA. Ref.2

Domain

Modular protein possessing six classical catalytic domains and a type III polyketide synthase domain. May facilitate covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization.

Disruption phenotype

Developed to the slug stage, in which dif-1 accumulation is maximal, though the Steely2- mutant slugs were thin and tended to break up. However, two independent Steely2 mutant strains both failed to accumulate any detectable dif-1 at this or any other stage of development. Ref.2

Miscellaneous

Encoded by one of the numerous copies of polyketide synthase genes localized in chromosome 5.

In reference to their hybrid nature and to their discovery in D.discoideum, authors (Ref.2) term these type I FAS-type III PKS fusion enzymes 'steely'.

Sequence similarities

Contains 1 acyl carrier domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 29682968Probable polyketide synthase 37
PRO_0000377902

Regions

Transmembrane1839 – 185921Helical; Potential
Transmembrane1869 – 188921Helical; Potential
Domain2426 – 249570Acyl carrier
Region179 – 23254Beta-ketoacyl synthase
Region708 – 74235Acyl/malonyl transferase
Region2707 – 2968262Chalcone synthase
Compositional bias2 – 2827Asn-rich
Compositional bias465 – 4695Poly-Thr
Compositional bias475 – 4795Poly-Ser
Compositional bias585 – 5884Poly-Asn
Compositional bias597 – 60812Poly-Asn
Compositional bias609 – 6179Poly-Ser
Compositional bias618 – 6214Poly-Asn
Compositional bias674 – 6774Poly-Gly
Compositional bias978 – 9825Poly-Gln
Compositional bias1076 – 10827Poly-Thr
Compositional bias1170 – 118011Poly-Thr
Compositional bias1346 – 135510Poly-Ser
Compositional bias1524 – 15285Poly-Gly
Compositional bias1535 – 15439Poly-Ser
Compositional bias1683 – 16864Poly-Ser
Compositional bias1821 – 18266Poly-Ser
Compositional bias2376 – 23816Poly-Gly
Compositional bias2567 – 25715Poly-Ser

Sites

Active site1981For beta-ketoacyl synthase activity By similarity
Active site7181For acyl/malonyl transferase activity By similarity
Active site27471 By similarity

Amino acid modifications

Modified residue24581O-(pantetheine 4'-phosphoryl)serine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54FI3 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 98AA21F6256A8A57

FASTA2,968329,909
        10         20         30         40         50         60 
MNNNKSINDL SGNSNNNIAN SNINNYNNLI KKEPIAIIGI GCRFPGNVSN YSDFVNIIKN 

        70         80         90        100        110        120 
GSDCLTKIPD DRWNADIISR KQWKLNNRIG GYLKNIDQFD NQFFGISPKE AQHIDPQQRL 

       130        140        150        160        170        180 
LLHLAIETLE DGKISLDEIK GKKVGVFIGS SSGDYLRGFD SSEINQFTTP GTNSSFLSNR 

       190        200        210        220        230        240 
LSYFLDVNGP SMTVNTACSA SMVAIHLGLQ SLWNGESELS MVGGVNIISS PLQSLDFGKA 

       250        260        270        280        290        300 
GLLNQETDGR CYSFDPRASG YVRSEGGGIL LLKPLSAALR DNDEIYSLLL NSANNSNGKT 

       310        320        330        340        350        360 
PTGITSPRSL CQEKLIQQLL RESSDQFSID DIGYFECHGT GTQMGDLNEI TAIGKSIGML 

       370        380        390        400        410        420 
KSHDDPLIIG SVKASIGHLE GASGICGVIK SIICLKEKIL PQQCKFSSYN PKIPFETLNL 

       430        440        450        460        470        480 
KVLTKTQPWN NSKRICGVNS FGVGGSNSSL FLSSFDKSTT ITEPTTTTTI ESLPSSSSSF 

       490        500        510        520        530        540 
DNLSVSSSIS TNNDNDKVSN IVNNRYGSSI DVITLSVTSP DKEDLKIRAN DVLESIKTLD 

       550        560        570        580        590        600 
DNFKIRDISN LTNIRTSHFS NRVAIIGDSI DSIKLNLQSF IKGENNNNKS IILPLINNGN 

       610        620        630        640        650        660 
NNNNNNNNSS GSSSSSSNNN NICFIFSGQG QQWNKMIFDL YENNKTFKNE MNNFSKQFEM 

       670        680        690        700        710        720 
ISGWSIIDKL YNSGGGGNEE LINETWLAQP SIVAVQYSLI KLFSKDIGIE GSIVLGHSLG 

       730        740        750        760        770        780 
ELMAAYYCGI INDFNDLLKL LYIRSTLQNK TNGSGRMHVC LSSKAEIEQL ISQLGFNGRI 

       790        800        810        820        830        840 
VICGNNTMKS CTISGDNESM NQFTKLISSQ QYGSVVHKEV RTNSAFHSHQ MDIIKDEFFK 

       850        860        870        880        890        900 
LFNQYFPTNQ ISTNQIYDGK SFYSTCYGKY LTPIECKQLL SSPNYWWKNI RESVLFKESI 

       910        920        930        940        950        960 
EQILQNHQQS LTFIEITCHP ILNYFLSQLL KSSSKSNTLL LSTLSKNSNS IDQLLILCSK 

       970        980        990       1000       1010       1020 
LYVNNLSSIK WNWFYDKQQQ QQSESLVSSN FKLPGRRWKL EKYWIENCQR QMDRIKPPMF 

      1030       1040       1050       1060       1070       1080 
ISLDRKLFSV TPSFEVRLNQ DRFQYLNDHQ IQDIPLVPFS FYIELVYASI FNSISTTTTN 

      1090       1100       1110       1120       1130       1140 
TTASTMFEIE NFTIDSSIII DQKKSTLIGI NFNSDLTKFE IGSINSIGSG SSSNNNFIEN 

      1150       1160       1170       1180       1190       1200 
KWKIHSNGII KYGTNYLKSN SKSNSFNEST TTTTTTTTTT KCFKSFNSNE FYNEIIKYNY 

      1210       1220       1230       1240       1250       1260 
NYKSTFQCVK EFKQFDKQGT FYYSEIQFKK NDKQVIDQLL SKQLPSDFRC IHPCLLDAVL 

      1270       1280       1290       1300       1310       1320 
QSAIIPATNK TNCSWIPIKI GKLSVNIPSN SYFNFKDQLL YCLIKPSTST STSPSTYFSS 

      1330       1340       1350       1360       1370       1380 
DIQVFDKKNN NLICELTNLE FKGINSSSSS SSSSSTINSN VEANYESKIE ETNHDEDEDE 

      1390       1400       1410       1420       1430       1440 
ELPLVSEYVW CKEELINQSI KFTDNYQTVI FCSTNLNGND LLDSIITSAL ENGHDENKIF 

      1450       1460       1470       1480       1490       1500 
IVSPPPVESD QYNNRIIINY TNNESDFDAL FAIINSTTSI SGKSGLFSTR FIILPNFNSI 

      1510       1520       1530       1540       1550       1560 
TFSSGNSTPL ITNVNGNGNG KSCGGGGGST NNTISNSSSS ISSIDNGNNE DEEMVLKSFN 

      1570       1580       1590       1600       1610       1620 
DSNLSLFHLQ KSIIKNNIKG RLFLITNGGQ SISSSTPTST YNDQSYVNLS QYQLIGQIRV 

      1630       1640       1650       1660       1670       1680 
FSNEYPIMEC SMIDIQDSTR IDLITDQLNS TKLSKLEIAF RDNIGYSYKL LKPSIFDNSS 

      1690       1700       1710       1720       1730       1740 
LPSSSSEIET TATTKDEEKN NSINYNNNYY RVELSDNGII SDLKIKQFRQ MKCGVGQVLV 

      1750       1760       1770       1780       1790       1800 
RVEMCTLNFR DILKSLGRDY DPIHLNSMGD EFSGKVIEIG EGVNNLSVGQ YVFGINMSKS 

      1810       1820       1830       1840       1850       1860 
MGSFVCCNSD LVFPIPIPTP SSSSSSNENI DDQEIISKLL NQYCTIPIVF LTSWYSIVIQ 

      1870       1880       1890       1900       1910       1920 
GRLKKGEKIL IHSGCGGVGL ATIQISMMIG AEIHVTVGSN EKKQYLIKEF GIDEKRIYSS 

      1930       1940       1950       1960       1970       1980 
RSLQFYNDLM VNTDGQGVDM VLNSLSGEYL EKSIQCLSQY GRFIEIGKKD IYSNSSIHLE 

      1990       2000       2010       2020       2030       2040 
PFKNNLSFFA VDIAQMTENR RDYLREIMID QLLPCFKNGS LKPLNQHCFN SPCDLVKAIR 

      2050       2060       2070       2080       2090       2100 
FMSSGNHIGK ILINWSNLNN DKQFINHHSV VHLPIQSFSN RSTYIFTGFG GLTQTLLKYF 

      2110       2120       2130       2140       2150       2160 
STESDLTNVI IVSKNGLDDN SGSGSGNNEK LKLINQLKES GLNVLVEKCD LSSIKQVYKL 

      2170       2180       2190       2200       2210       2220 
FNKIFDNDAS GSDSGDFSDI KGIFHFASLI NDKRILKHNL ESFNYVYNSK ATSAWNLHQV 

      2230       2240       2250       2260       2270       2280 
SLKYNLNLDH FQTIGSVITI LGNIGQSNYT CANRFVEGLT HLRIGMGLKS SCIHLASIPD 

      2290       2300       2310       2320       2330       2340 
VGMASNDNVL NDLNSMGFVP FQSLNEMNLG FKKLLSSPNP IVVLGEINVD RFIEATPNFR 

      2350       2360       2370       2380       2390       2400 
AKDNFIITSL FNRIDPLLLV NESQDFIINN NINNNGGGGD GSFDDLNQLE DEGQQGFGNG 

      2410       2420       2430       2440       2450       2460 
DGYVDDNIDS VSMLSGTSSI FDNDFYTKSI RGMLCDILEL KDKDLNNTVS FSDYGLDSLL 

      2470       2480       2490       2500       2510       2520 
SSELSNTIQK NFSILIPSLT LVDNSTINST VELIKNKLKN STTSSISSSV SKKVSFKKNT 

      2530       2540       2550       2560       2570       2580 
QPLIIPTTAP ISIIKTQSYI KSEIIESLPI SSSTTIKPLV FDNLVYSSSS SNNSNSKNEL 

      2590       2600       2610       2620       2630       2640 
TSPPPSAKRE SVLPIISEDN NSDNDSSMAT VIYEISPIAA PYHRYQTDVL KEITQLTPHK 

      2650       2660       2670       2680       2690       2700 
EFIDNIYKKS KIRSRYCFND FSEKSMADIN KLDAGERVAL FREQTYQTVI NAGKTVIERA 

      2710       2720       2730       2740       2750       2760 
GIDPMLISHV VGVTSTGIMA PSFDVVLIDK LGLSINTSRT MINFMGCGAA VNSMRAATAY 

      2770       2780       2790       2800       2810       2820 
AKLKPGTFVL VVAVEASATC MKFNFDSRSD LLSQAIFTDG CVATLVTCQP KSSLVGKLEI 

      2830       2840       2850       2860       2870       2880 
IDDLSYLMPD SRDALNLFIG PTGIDLDLRP ELPIAINRHI NSAITSWLKK NSLQKSDIEF 

      2890       2900       2910       2920       2930       2940 
FATHPGGAKI ISAVHEGLGL SPEDLSDSYE VMKRYGNMIG VSTYYVLRRI LDKNQTLLQE 

      2950       2960 
GSLGYNYGMA MAFSPGASIE AILFKLIK 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase."
Austin M.B., Saito T., Bowman M.E., Haydock S., Kato A., Moore B.S., Kay R.R., Noel J.P.
Nat. Chem. Biol. 2:494-502(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[3]"Polyketide synthase genes and the natural products potential of Dictyostelium discoideum."
Zucko J., Skunca N., Curk T., Zupan B., Long P.F., Cullum J., Kessin R.H., Hranueli D.
Bioinformatics 23:2543-2549(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000171 Genomic DNA. Translation: EAL62021.1.

3D structure databases

ProteinModelPortalQ54FI3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0219613.

Proteomic databases

PRIDEQ54FI3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0234163; DDB0234163; DDB_G0290853.
KEGGddi:DDB_G0290853.

Organism-specific databases

dictyBaseDDB_G0290853. stlB.

Phylogenomic databases

eggNOGCOG3424.
InParanoidQ54FI3.
OMAHINSAIT.
PhylomeDBQ54FI3.

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 4 hits.
3.40.50.720. 2 hits.
3.90.180.10. 1 hit.
InterProIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF00698. Acyl_transf_1. 1 hit.
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMSSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 4 hits.
PROSITEPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePKS37_DICDI
AccessionPrimary (citable) accession number: Q54FI3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase