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Q54FI3

- PKS37_DICDI

UniProt

Q54FI3 - PKS37_DICDI

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Protein
Probable polyketide synthase 37
Gene
stlB, pks37, DDB_G0290853
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Does make the polyketide skeleton of dif-1.1 Publication

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Cofactori

Binds 1 phosphopantetheine covalently By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981For beta-ketoacyl synthase activity By similarity
Active sitei718 – 7181For acyl/malonyl transferase activity By similarity
Active sitei2747 – 27471 By similarity

GO - Molecular functioni

  1. naringenin-chalcone synthase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. DIF-1 biosynthetic process Source: dictyBase
  2. flavonoid biosynthetic process Source: UniProtKB-UniPathway
  3. polyketide biosynthetic process Source: dictyBase
  4. sorocarp morphogenesis Source: dictyBase
  5. sorocarp stalk cell differentiation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable polyketide synthase 37
Short name:
dipks37
Including the following 2 domains:
Polyketide synthase stlB (EC:2.3.1.-)
Chalcone synthase stlB (EC:2.3.1.74)
Alternative name(s):
Steely2
Gene namesi
Name:stlB
Synonyms:pks37
ORF Names:DDB_G0290853
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290853. stlB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1839 – 185921Helical; Reviewed prediction
Add
BLAST
Transmembranei1869 – 188921Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intracellular Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Developed to the slug stage, in which dif-1 accumulation is maximal, though the Steely2- mutant slugs were thin and tended to break up. However, two independent Steely2 mutant strains both failed to accumulate any detectable dif-1 at this or any other stage of development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 29682968Probable polyketide synthase 37
PRO_0000377902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2458 – 24581O-(pantetheine 4'-phosphoryl)serine By similarity

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PRIDEiQ54FI3.

Expressioni

Developmental stagei

Expressed during development. Coordinately expressed with dmtA.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0219613.

Structurei

3D structure databases

ProteinModelPortaliQ54FI3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2426 – 249570Acyl carrier
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni179 – 23254Beta-ketoacyl synthase
Add
BLAST
Regioni708 – 74235Acyl/malonyl transferase
Add
BLAST
Regioni2707 – 2968262Chalcone synthase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 2827Asn-rich
Add
BLAST
Compositional biasi465 – 4695Poly-Thr
Compositional biasi475 – 4795Poly-Ser
Compositional biasi585 – 5884Poly-Asn
Compositional biasi597 – 60812Poly-Asn
Add
BLAST
Compositional biasi609 – 6179Poly-Ser
Compositional biasi618 – 6214Poly-Asn
Compositional biasi674 – 6774Poly-Gly
Compositional biasi978 – 9825Poly-Gln
Compositional biasi1076 – 10827Poly-Thr
Compositional biasi1170 – 118011Poly-Thr
Add
BLAST
Compositional biasi1346 – 135510Poly-Ser
Compositional biasi1524 – 15285Poly-Gly
Compositional biasi1535 – 15439Poly-Ser
Compositional biasi1683 – 16864Poly-Ser
Compositional biasi1821 – 18266Poly-Ser
Compositional biasi2376 – 23816Poly-Gly
Compositional biasi2567 – 25715Poly-Ser

Domaini

Modular protein possessing six classical catalytic domains and a type III polyketide synthase domain. May facilitate covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3424.
InParanoidiQ54FI3.
OMAiHINSAIT.
PhylomeDBiQ54FI3.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 4 hits.
3.40.50.720. 2 hits.
3.90.180.10. 1 hit.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 4 hits.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54FI3-1 [UniParc]FASTAAdd to Basket

« Hide

MNNNKSINDL SGNSNNNIAN SNINNYNNLI KKEPIAIIGI GCRFPGNVSN     50
YSDFVNIIKN GSDCLTKIPD DRWNADIISR KQWKLNNRIG GYLKNIDQFD 100
NQFFGISPKE AQHIDPQQRL LLHLAIETLE DGKISLDEIK GKKVGVFIGS 150
SSGDYLRGFD SSEINQFTTP GTNSSFLSNR LSYFLDVNGP SMTVNTACSA 200
SMVAIHLGLQ SLWNGESELS MVGGVNIISS PLQSLDFGKA GLLNQETDGR 250
CYSFDPRASG YVRSEGGGIL LLKPLSAALR DNDEIYSLLL NSANNSNGKT 300
PTGITSPRSL CQEKLIQQLL RESSDQFSID DIGYFECHGT GTQMGDLNEI 350
TAIGKSIGML KSHDDPLIIG SVKASIGHLE GASGICGVIK SIICLKEKIL 400
PQQCKFSSYN PKIPFETLNL KVLTKTQPWN NSKRICGVNS FGVGGSNSSL 450
FLSSFDKSTT ITEPTTTTTI ESLPSSSSSF DNLSVSSSIS TNNDNDKVSN 500
IVNNRYGSSI DVITLSVTSP DKEDLKIRAN DVLESIKTLD DNFKIRDISN 550
LTNIRTSHFS NRVAIIGDSI DSIKLNLQSF IKGENNNNKS IILPLINNGN 600
NNNNNNNNSS GSSSSSSNNN NICFIFSGQG QQWNKMIFDL YENNKTFKNE 650
MNNFSKQFEM ISGWSIIDKL YNSGGGGNEE LINETWLAQP SIVAVQYSLI 700
KLFSKDIGIE GSIVLGHSLG ELMAAYYCGI INDFNDLLKL LYIRSTLQNK 750
TNGSGRMHVC LSSKAEIEQL ISQLGFNGRI VICGNNTMKS CTISGDNESM 800
NQFTKLISSQ QYGSVVHKEV RTNSAFHSHQ MDIIKDEFFK LFNQYFPTNQ 850
ISTNQIYDGK SFYSTCYGKY LTPIECKQLL SSPNYWWKNI RESVLFKESI 900
EQILQNHQQS LTFIEITCHP ILNYFLSQLL KSSSKSNTLL LSTLSKNSNS 950
IDQLLILCSK LYVNNLSSIK WNWFYDKQQQ QQSESLVSSN FKLPGRRWKL 1000
EKYWIENCQR QMDRIKPPMF ISLDRKLFSV TPSFEVRLNQ DRFQYLNDHQ 1050
IQDIPLVPFS FYIELVYASI FNSISTTTTN TTASTMFEIE NFTIDSSIII 1100
DQKKSTLIGI NFNSDLTKFE IGSINSIGSG SSSNNNFIEN KWKIHSNGII 1150
KYGTNYLKSN SKSNSFNEST TTTTTTTTTT KCFKSFNSNE FYNEIIKYNY 1200
NYKSTFQCVK EFKQFDKQGT FYYSEIQFKK NDKQVIDQLL SKQLPSDFRC 1250
IHPCLLDAVL QSAIIPATNK TNCSWIPIKI GKLSVNIPSN SYFNFKDQLL 1300
YCLIKPSTST STSPSTYFSS DIQVFDKKNN NLICELTNLE FKGINSSSSS 1350
SSSSSTINSN VEANYESKIE ETNHDEDEDE ELPLVSEYVW CKEELINQSI 1400
KFTDNYQTVI FCSTNLNGND LLDSIITSAL ENGHDENKIF IVSPPPVESD 1450
QYNNRIIINY TNNESDFDAL FAIINSTTSI SGKSGLFSTR FIILPNFNSI 1500
TFSSGNSTPL ITNVNGNGNG KSCGGGGGST NNTISNSSSS ISSIDNGNNE 1550
DEEMVLKSFN DSNLSLFHLQ KSIIKNNIKG RLFLITNGGQ SISSSTPTST 1600
YNDQSYVNLS QYQLIGQIRV FSNEYPIMEC SMIDIQDSTR IDLITDQLNS 1650
TKLSKLEIAF RDNIGYSYKL LKPSIFDNSS LPSSSSEIET TATTKDEEKN 1700
NSINYNNNYY RVELSDNGII SDLKIKQFRQ MKCGVGQVLV RVEMCTLNFR 1750
DILKSLGRDY DPIHLNSMGD EFSGKVIEIG EGVNNLSVGQ YVFGINMSKS 1800
MGSFVCCNSD LVFPIPIPTP SSSSSSNENI DDQEIISKLL NQYCTIPIVF 1850
LTSWYSIVIQ GRLKKGEKIL IHSGCGGVGL ATIQISMMIG AEIHVTVGSN 1900
EKKQYLIKEF GIDEKRIYSS RSLQFYNDLM VNTDGQGVDM VLNSLSGEYL 1950
EKSIQCLSQY GRFIEIGKKD IYSNSSIHLE PFKNNLSFFA VDIAQMTENR 2000
RDYLREIMID QLLPCFKNGS LKPLNQHCFN SPCDLVKAIR FMSSGNHIGK 2050
ILINWSNLNN DKQFINHHSV VHLPIQSFSN RSTYIFTGFG GLTQTLLKYF 2100
STESDLTNVI IVSKNGLDDN SGSGSGNNEK LKLINQLKES GLNVLVEKCD 2150
LSSIKQVYKL FNKIFDNDAS GSDSGDFSDI KGIFHFASLI NDKRILKHNL 2200
ESFNYVYNSK ATSAWNLHQV SLKYNLNLDH FQTIGSVITI LGNIGQSNYT 2250
CANRFVEGLT HLRIGMGLKS SCIHLASIPD VGMASNDNVL NDLNSMGFVP 2300
FQSLNEMNLG FKKLLSSPNP IVVLGEINVD RFIEATPNFR AKDNFIITSL 2350
FNRIDPLLLV NESQDFIINN NINNNGGGGD GSFDDLNQLE DEGQQGFGNG 2400
DGYVDDNIDS VSMLSGTSSI FDNDFYTKSI RGMLCDILEL KDKDLNNTVS 2450
FSDYGLDSLL SSELSNTIQK NFSILIPSLT LVDNSTINST VELIKNKLKN 2500
STTSSISSSV SKKVSFKKNT QPLIIPTTAP ISIIKTQSYI KSEIIESLPI 2550
SSSTTIKPLV FDNLVYSSSS SNNSNSKNEL TSPPPSAKRE SVLPIISEDN 2600
NSDNDSSMAT VIYEISPIAA PYHRYQTDVL KEITQLTPHK EFIDNIYKKS 2650
KIRSRYCFND FSEKSMADIN KLDAGERVAL FREQTYQTVI NAGKTVIERA 2700
GIDPMLISHV VGVTSTGIMA PSFDVVLIDK LGLSINTSRT MINFMGCGAA 2750
VNSMRAATAY AKLKPGTFVL VVAVEASATC MKFNFDSRSD LLSQAIFTDG 2800
CVATLVTCQP KSSLVGKLEI IDDLSYLMPD SRDALNLFIG PTGIDLDLRP 2850
ELPIAINRHI NSAITSWLKK NSLQKSDIEF FATHPGGAKI ISAVHEGLGL 2900
SPEDLSDSYE VMKRYGNMIG VSTYYVLRRI LDKNQTLLQE GSLGYNYGMA 2950
MAFSPGASIE AILFKLIK 2968
Length:2,968
Mass (Da):329,909
Last modified:May 24, 2005 - v1
Checksum:i98AA21F6256A8A57
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000171 Genomic DNA. Translation: EAL62021.1.
RefSeqiXP_635518.1. XM_630426.1.

Genome annotation databases

EnsemblProtistsiDDB0234163; DDB0234163; DDB_G0290853.
GeneIDi8627855.
KEGGiddi:DDB_G0290853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000171 Genomic DNA. Translation: EAL62021.1 .
RefSeqi XP_635518.1. XM_630426.1.

3D structure databases

ProteinModelPortali Q54FI3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0219613.

Proteomic databases

PRIDEi Q54FI3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0234163 ; DDB0234163 ; DDB_G0290853 .
GeneIDi 8627855.
KEGGi ddi:DDB_G0290853.

Organism-specific databases

dictyBasei DDB_G0290853. stlB.

Phylogenomic databases

eggNOGi COG3424.
InParanoidi Q54FI3.
OMAi HINSAIT.
PhylomeDBi Q54FI3.

Enzyme and pathway databases

UniPathwayi UPA00154 .

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 4 hits.
3.40.50.720. 2 hits.
3.90.180.10. 1 hit.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
SMARTi SM00829. PKS_ER. 1 hit.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 4 hits.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase."
    Austin M.B., Saito T., Bowman M.E., Haydock S., Kato A., Moore B.S., Kay R.R., Noel J.P.
    Nat. Chem. Biol. 2:494-502(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  3. "Polyketide synthase genes and the natural products potential of Dictyostelium discoideum."
    Zucko J., Skunca N., Curk T., Zupan B., Long P.F., Cullum J., Kessin R.H., Hranueli D.
    Bioinformatics 23:2543-2549(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiPKS37_DICDI
AccessioniPrimary (citable) accession number: Q54FI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 24, 2005
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Encoded by one of the numerous copies of polyketide synthase genes localized in chromosome 5.
In reference to their hybrid nature and to their discovery in D.discoideum, authors (1 Publication) term these type I FAS-type III PKS fusion enzymes 'steely'.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3