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Q54FI3

- PKS37_DICDI

UniProt

Q54FI3 - PKS37_DICDI

Protein

Probable polyketide synthase 37

Gene

stlB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Does make the polyketide skeleton of dif-1.1 Publication

    Catalytic activityi

    3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

    Cofactori

    Binds 1 phosphopantetheine covalently.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei198 – 1981For beta-ketoacyl synthase activityPROSITE-ProRule annotation
    Active sitei718 – 7181For acyl/malonyl transferase activityPROSITE-ProRule annotation
    Active sitei2747 – 27471PROSITE-ProRule annotation

    GO - Molecular functioni

    1. naringenin-chalcone synthase activity Source: UniProtKB-EC
    2. oxidoreductase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. DIF-1 biosynthetic process Source: dictyBase
    2. flavonoid biosynthetic process Source: UniProtKB-UniPathway
    3. polyketide biosynthetic process Source: dictyBase
    4. sorocarp morphogenesis Source: dictyBase
    5. sorocarp stalk cell differentiation Source: dictyBase

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00154.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable polyketide synthase 37
    Short name:
    dipks37
    Including the following 2 domains:
    Polyketide synthase stlB (EC:2.3.1.-)
    Chalcone synthase stlB (EC:2.3.1.74)
    Alternative name(s):
    Steely2
    Gene namesi
    Name:stlB
    Synonyms:pks37
    ORF Names:DDB_G0290853
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0290853. stlB.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. intracellular Source: dictyBase

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Developed to the slug stage, in which dif-1 accumulation is maximal, though the Steely2- mutant slugs were thin and tended to break up. However, two independent Steely2 mutant strains both failed to accumulate any detectable dif-1 at this or any other stage of development.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 29682968Probable polyketide synthase 37PRO_0000377902Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2458 – 24581O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

    Keywords - PTMi

    Phosphopantetheine, Phosphoprotein

    Proteomic databases

    PRIDEiQ54FI3.

    Expressioni

    Developmental stagei

    Expressed during development. Coordinately expressed with dmtA.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0219613.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54FI3.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1839 – 185921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei1869 – 188921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2426 – 249570Acyl carrierPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni179 – 23254Beta-ketoacyl synthaseAdd
    BLAST
    Regioni708 – 74235Acyl/malonyl transferaseAdd
    BLAST
    Regioni2707 – 2968262Chalcone synthaseAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi2 – 2827Asn-richAdd
    BLAST
    Compositional biasi465 – 4695Poly-Thr
    Compositional biasi475 – 4795Poly-Ser
    Compositional biasi585 – 5884Poly-Asn
    Compositional biasi597 – 60812Poly-AsnAdd
    BLAST
    Compositional biasi609 – 6179Poly-Ser
    Compositional biasi618 – 6214Poly-Asn
    Compositional biasi674 – 6774Poly-Gly
    Compositional biasi978 – 9825Poly-Gln
    Compositional biasi1076 – 10827Poly-Thr
    Compositional biasi1170 – 118011Poly-ThrAdd
    BLAST
    Compositional biasi1346 – 135510Poly-Ser
    Compositional biasi1524 – 15285Poly-Gly
    Compositional biasi1535 – 15439Poly-Ser
    Compositional biasi1683 – 16864Poly-Ser
    Compositional biasi1821 – 18266Poly-Ser
    Compositional biasi2376 – 23816Poly-Gly
    Compositional biasi2567 – 25715Poly-Ser

    Domaini

    Modular protein possessing six classical catalytic domains and a type III polyketide synthase domain. May facilitate covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization.

    Sequence similaritiesi

    Contains 1 acyl carrier domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3424.
    InParanoidiQ54FI3.
    OMAiHINSAIT.
    PhylomeDBiQ54FI3.

    Family and domain databases

    Gene3Di1.10.1200.10. 1 hit.
    3.40.366.10. 2 hits.
    3.40.47.10. 4 hits.
    3.40.50.720. 2 hits.
    3.90.180.10. 1 hit.
    InterProiIPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR012328. Chalcone/stilbene_synth_C.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011032. GroES-like.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016040. NAD(P)-bd_dom.
    IPR020843. PKS_ER.
    IPR013968. PKS_KR.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00698. Acyl_transf_1. 1 hit.
    PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    PF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view]
    SMARTiSM00829. PKS_ER. 1 hit.
    [Graphical view]
    SUPFAMiSSF47336. SSF47336. 1 hit.
    SSF50129. SSF50129. 2 hits.
    SSF52151. SSF52151. 2 hits.
    SSF53901. SSF53901. 4 hits.
    PROSITEiPS50075. ACP_DOMAIN. 1 hit.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54FI3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNNKSINDL SGNSNNNIAN SNINNYNNLI KKEPIAIIGI GCRFPGNVSN     50
    YSDFVNIIKN GSDCLTKIPD DRWNADIISR KQWKLNNRIG GYLKNIDQFD 100
    NQFFGISPKE AQHIDPQQRL LLHLAIETLE DGKISLDEIK GKKVGVFIGS 150
    SSGDYLRGFD SSEINQFTTP GTNSSFLSNR LSYFLDVNGP SMTVNTACSA 200
    SMVAIHLGLQ SLWNGESELS MVGGVNIISS PLQSLDFGKA GLLNQETDGR 250
    CYSFDPRASG YVRSEGGGIL LLKPLSAALR DNDEIYSLLL NSANNSNGKT 300
    PTGITSPRSL CQEKLIQQLL RESSDQFSID DIGYFECHGT GTQMGDLNEI 350
    TAIGKSIGML KSHDDPLIIG SVKASIGHLE GASGICGVIK SIICLKEKIL 400
    PQQCKFSSYN PKIPFETLNL KVLTKTQPWN NSKRICGVNS FGVGGSNSSL 450
    FLSSFDKSTT ITEPTTTTTI ESLPSSSSSF DNLSVSSSIS TNNDNDKVSN 500
    IVNNRYGSSI DVITLSVTSP DKEDLKIRAN DVLESIKTLD DNFKIRDISN 550
    LTNIRTSHFS NRVAIIGDSI DSIKLNLQSF IKGENNNNKS IILPLINNGN 600
    NNNNNNNNSS GSSSSSSNNN NICFIFSGQG QQWNKMIFDL YENNKTFKNE 650
    MNNFSKQFEM ISGWSIIDKL YNSGGGGNEE LINETWLAQP SIVAVQYSLI 700
    KLFSKDIGIE GSIVLGHSLG ELMAAYYCGI INDFNDLLKL LYIRSTLQNK 750
    TNGSGRMHVC LSSKAEIEQL ISQLGFNGRI VICGNNTMKS CTISGDNESM 800
    NQFTKLISSQ QYGSVVHKEV RTNSAFHSHQ MDIIKDEFFK LFNQYFPTNQ 850
    ISTNQIYDGK SFYSTCYGKY LTPIECKQLL SSPNYWWKNI RESVLFKESI 900
    EQILQNHQQS LTFIEITCHP ILNYFLSQLL KSSSKSNTLL LSTLSKNSNS 950
    IDQLLILCSK LYVNNLSSIK WNWFYDKQQQ QQSESLVSSN FKLPGRRWKL 1000
    EKYWIENCQR QMDRIKPPMF ISLDRKLFSV TPSFEVRLNQ DRFQYLNDHQ 1050
    IQDIPLVPFS FYIELVYASI FNSISTTTTN TTASTMFEIE NFTIDSSIII 1100
    DQKKSTLIGI NFNSDLTKFE IGSINSIGSG SSSNNNFIEN KWKIHSNGII 1150
    KYGTNYLKSN SKSNSFNEST TTTTTTTTTT KCFKSFNSNE FYNEIIKYNY 1200
    NYKSTFQCVK EFKQFDKQGT FYYSEIQFKK NDKQVIDQLL SKQLPSDFRC 1250
    IHPCLLDAVL QSAIIPATNK TNCSWIPIKI GKLSVNIPSN SYFNFKDQLL 1300
    YCLIKPSTST STSPSTYFSS DIQVFDKKNN NLICELTNLE FKGINSSSSS 1350
    SSSSSTINSN VEANYESKIE ETNHDEDEDE ELPLVSEYVW CKEELINQSI 1400
    KFTDNYQTVI FCSTNLNGND LLDSIITSAL ENGHDENKIF IVSPPPVESD 1450
    QYNNRIIINY TNNESDFDAL FAIINSTTSI SGKSGLFSTR FIILPNFNSI 1500
    TFSSGNSTPL ITNVNGNGNG KSCGGGGGST NNTISNSSSS ISSIDNGNNE 1550
    DEEMVLKSFN DSNLSLFHLQ KSIIKNNIKG RLFLITNGGQ SISSSTPTST 1600
    YNDQSYVNLS QYQLIGQIRV FSNEYPIMEC SMIDIQDSTR IDLITDQLNS 1650
    TKLSKLEIAF RDNIGYSYKL LKPSIFDNSS LPSSSSEIET TATTKDEEKN 1700
    NSINYNNNYY RVELSDNGII SDLKIKQFRQ MKCGVGQVLV RVEMCTLNFR 1750
    DILKSLGRDY DPIHLNSMGD EFSGKVIEIG EGVNNLSVGQ YVFGINMSKS 1800
    MGSFVCCNSD LVFPIPIPTP SSSSSSNENI DDQEIISKLL NQYCTIPIVF 1850
    LTSWYSIVIQ GRLKKGEKIL IHSGCGGVGL ATIQISMMIG AEIHVTVGSN 1900
    EKKQYLIKEF GIDEKRIYSS RSLQFYNDLM VNTDGQGVDM VLNSLSGEYL 1950
    EKSIQCLSQY GRFIEIGKKD IYSNSSIHLE PFKNNLSFFA VDIAQMTENR 2000
    RDYLREIMID QLLPCFKNGS LKPLNQHCFN SPCDLVKAIR FMSSGNHIGK 2050
    ILINWSNLNN DKQFINHHSV VHLPIQSFSN RSTYIFTGFG GLTQTLLKYF 2100
    STESDLTNVI IVSKNGLDDN SGSGSGNNEK LKLINQLKES GLNVLVEKCD 2150
    LSSIKQVYKL FNKIFDNDAS GSDSGDFSDI KGIFHFASLI NDKRILKHNL 2200
    ESFNYVYNSK ATSAWNLHQV SLKYNLNLDH FQTIGSVITI LGNIGQSNYT 2250
    CANRFVEGLT HLRIGMGLKS SCIHLASIPD VGMASNDNVL NDLNSMGFVP 2300
    FQSLNEMNLG FKKLLSSPNP IVVLGEINVD RFIEATPNFR AKDNFIITSL 2350
    FNRIDPLLLV NESQDFIINN NINNNGGGGD GSFDDLNQLE DEGQQGFGNG 2400
    DGYVDDNIDS VSMLSGTSSI FDNDFYTKSI RGMLCDILEL KDKDLNNTVS 2450
    FSDYGLDSLL SSELSNTIQK NFSILIPSLT LVDNSTINST VELIKNKLKN 2500
    STTSSISSSV SKKVSFKKNT QPLIIPTTAP ISIIKTQSYI KSEIIESLPI 2550
    SSSTTIKPLV FDNLVYSSSS SNNSNSKNEL TSPPPSAKRE SVLPIISEDN 2600
    NSDNDSSMAT VIYEISPIAA PYHRYQTDVL KEITQLTPHK EFIDNIYKKS 2650
    KIRSRYCFND FSEKSMADIN KLDAGERVAL FREQTYQTVI NAGKTVIERA 2700
    GIDPMLISHV VGVTSTGIMA PSFDVVLIDK LGLSINTSRT MINFMGCGAA 2750
    VNSMRAATAY AKLKPGTFVL VVAVEASATC MKFNFDSRSD LLSQAIFTDG 2800
    CVATLVTCQP KSSLVGKLEI IDDLSYLMPD SRDALNLFIG PTGIDLDLRP 2850
    ELPIAINRHI NSAITSWLKK NSLQKSDIEF FATHPGGAKI ISAVHEGLGL 2900
    SPEDLSDSYE VMKRYGNMIG VSTYYVLRRI LDKNQTLLQE GSLGYNYGMA 2950
    MAFSPGASIE AILFKLIK 2968
    Length:2,968
    Mass (Da):329,909
    Last modified:May 24, 2005 - v1
    Checksum:i98AA21F6256A8A57
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000171 Genomic DNA. Translation: EAL62021.1.
    RefSeqiXP_635518.1. XM_630426.1.

    Genome annotation databases

    EnsemblProtistsiDDB0234163; DDB0234163; DDB_G0290853.
    GeneIDi8627855.
    KEGGiddi:DDB_G0290853.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000171 Genomic DNA. Translation: EAL62021.1 .
    RefSeqi XP_635518.1. XM_630426.1.

    3D structure databases

    ProteinModelPortali Q54FI3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0219613.

    Proteomic databases

    PRIDEi Q54FI3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0234163 ; DDB0234163 ; DDB_G0290853 .
    GeneIDi 8627855.
    KEGGi ddi:DDB_G0290853.

    Organism-specific databases

    dictyBasei DDB_G0290853. stlB.

    Phylogenomic databases

    eggNOGi COG3424.
    InParanoidi Q54FI3.
    OMAi HINSAIT.
    PhylomeDBi Q54FI3.

    Enzyme and pathway databases

    UniPathwayi UPA00154 .

    Family and domain databases

    Gene3Di 1.10.1200.10. 1 hit.
    3.40.366.10. 2 hits.
    3.40.47.10. 4 hits.
    3.40.50.720. 2 hits.
    3.90.180.10. 1 hit.
    InterProi IPR001227. Ac_transferase_dom.
    IPR009081. Acyl_carrier_prot-like.
    IPR014043. Acyl_transferase.
    IPR016035. Acyl_Trfase/lysoPLipase.
    IPR013149. ADH_C.
    IPR013154. ADH_GroES-like.
    IPR012328. Chalcone/stilbene_synth_C.
    IPR001099. Chalcone/stilbene_synthase_N.
    IPR011032. GroES-like.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016040. NAD(P)-bd_dom.
    IPR020843. PKS_ER.
    IPR013968. PKS_KR.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00698. Acyl_transf_1. 1 hit.
    PF08240. ADH_N. 1 hit.
    PF00107. ADH_zinc_N. 1 hit.
    PF02797. Chal_sti_synt_C. 1 hit.
    PF00195. Chal_sti_synt_N. 1 hit.
    PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    PF08659. KR. 1 hit.
    PF00550. PP-binding. 1 hit.
    [Graphical view ]
    SMARTi SM00829. PKS_ER. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47336. SSF47336. 1 hit.
    SSF50129. SSF50129. 2 hits.
    SSF52151. SSF52151. 2 hits.
    SSF53901. SSF53901. 4 hits.
    PROSITEi PS50075. ACP_DOMAIN. 1 hit.
    PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase."
      Austin M.B., Saito T., Bowman M.E., Haydock S., Kato A., Moore B.S., Kay R.R., Noel J.P.
      Nat. Chem. Biol. 2:494-502(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    3. "Polyketide synthase genes and the natural products potential of Dictyostelium discoideum."
      Zucko J., Skunca N., Curk T., Zupan B., Long P.F., Cullum J., Kessin R.H., Hranueli D.
      Bioinformatics 23:2543-2549(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.

    Entry informationi

    Entry nameiPKS37_DICDI
    AccessioniPrimary (citable) accession number: Q54FI3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 16, 2009
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    Encoded by one of the numerous copies of polyketide synthase genes localized in chromosome 5.
    In reference to their hybrid nature and to their discovery in D.discoideum, authors term these type I FAS-type III PKS fusion enzymes 'steely'.1 Publication

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3