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Q54FI3

- PKS37_DICDI

UniProt

Q54FI3 - PKS37_DICDI

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Protein

Probable polyketide synthase 37

Gene

stlB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Does make the polyketide skeleton of dif-1.1 Publication

Catalytic activityi

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Cofactori

pantetheine 4'-phosphateBy similarityNote: Binds 1 phosphopantetheine covalently.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei198 – 1981For beta-ketoacyl synthase activityPROSITE-ProRule annotation
Active sitei718 – 7181For acyl/malonyl transferase activityPROSITE-ProRule annotation
Active sitei2747 – 27471PROSITE-ProRule annotation

GO - Molecular functioni

  1. naringenin-chalcone synthase activity Source: UniProtKB-EC
  2. oxidoreductase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. DIF-1 biosynthetic process Source: dictyBase
  2. flavonoid biosynthetic process Source: UniProtKB-UniPathway
  3. polyketide biosynthetic process Source: dictyBase
  4. sorocarp morphogenesis Source: dictyBase
  5. sorocarp stalk cell differentiation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00154.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable polyketide synthase 37
Short name:
dipks37
Including the following 2 domains:
Polyketide synthase stlB (EC:2.3.1.-)
Chalcone synthase stlB (EC:2.3.1.74)
Alternative name(s):
Steely2
Gene namesi
Name:stlB
Synonyms:pks37
ORF Names:DDB_G0290853
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0290853. stlB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1839 – 185921HelicalSequence AnalysisAdd
BLAST
Transmembranei1869 – 188921HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. intracellular Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Developed to the slug stage, in which dif-1 accumulation is maximal, though the Steely2- mutant slugs were thin and tended to break up. However, two independent Steely2 mutant strains both failed to accumulate any detectable dif-1 at this or any other stage of development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 29682968Probable polyketide synthase 37PRO_0000377902Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2458 – 24581O-(pantetheine 4'-phosphoryl)serinePROSITE-ProRule annotation

Keywords - PTMi

Phosphopantetheine, Phosphoprotein

Proteomic databases

PRIDEiQ54FI3.

Expressioni

Developmental stagei

Expressed during development. Coordinately expressed with dmtA.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0219613.

Structurei

3D structure databases

ProteinModelPortaliQ54FI3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2426 – 249570Acyl carrierPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni179 – 23254Beta-ketoacyl synthaseAdd
BLAST
Regioni708 – 74235Acyl/malonyl transferaseAdd
BLAST
Regioni2707 – 2968262Chalcone synthaseAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 2827Asn-richAdd
BLAST
Compositional biasi465 – 4695Poly-Thr
Compositional biasi475 – 4795Poly-Ser
Compositional biasi585 – 5884Poly-Asn
Compositional biasi597 – 60812Poly-AsnAdd
BLAST
Compositional biasi609 – 6179Poly-Ser
Compositional biasi618 – 6214Poly-Asn
Compositional biasi674 – 6774Poly-Gly
Compositional biasi978 – 9825Poly-Gln
Compositional biasi1076 – 10827Poly-Thr
Compositional biasi1170 – 118011Poly-ThrAdd
BLAST
Compositional biasi1346 – 135510Poly-Ser
Compositional biasi1524 – 15285Poly-Gly
Compositional biasi1535 – 15439Poly-Ser
Compositional biasi1683 – 16864Poly-Ser
Compositional biasi1821 – 18266Poly-Ser
Compositional biasi2376 – 23816Poly-Gly
Compositional biasi2567 – 25715Poly-Ser

Domaini

Modular protein possessing six classical catalytic domains and a type III polyketide synthase domain. May facilitate covalent transfer of steely N-terminal acyl products directly to the C-terminal type III PKS active sites, which catalyze both iterative polyketide extension and cyclization.

Sequence similaritiesi

Contains 1 acyl carrier domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3424.
InParanoidiQ54FI3.
OMAiHINSAIT.
PhylomeDBiQ54FI3.

Family and domain databases

Gene3Di1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 4 hits.
3.40.50.720. 2 hits.
3.90.180.10. 1 hit.
InterProiIPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00698. Acyl_transf_1. 1 hit.
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view]
SMARTiSM00829. PKS_ER. 1 hit.
[Graphical view]
SUPFAMiSSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 4 hits.
PROSITEiPS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54FI3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNNKSINDL SGNSNNNIAN SNINNYNNLI KKEPIAIIGI GCRFPGNVSN
60 70 80 90 100
YSDFVNIIKN GSDCLTKIPD DRWNADIISR KQWKLNNRIG GYLKNIDQFD
110 120 130 140 150
NQFFGISPKE AQHIDPQQRL LLHLAIETLE DGKISLDEIK GKKVGVFIGS
160 170 180 190 200
SSGDYLRGFD SSEINQFTTP GTNSSFLSNR LSYFLDVNGP SMTVNTACSA
210 220 230 240 250
SMVAIHLGLQ SLWNGESELS MVGGVNIISS PLQSLDFGKA GLLNQETDGR
260 270 280 290 300
CYSFDPRASG YVRSEGGGIL LLKPLSAALR DNDEIYSLLL NSANNSNGKT
310 320 330 340 350
PTGITSPRSL CQEKLIQQLL RESSDQFSID DIGYFECHGT GTQMGDLNEI
360 370 380 390 400
TAIGKSIGML KSHDDPLIIG SVKASIGHLE GASGICGVIK SIICLKEKIL
410 420 430 440 450
PQQCKFSSYN PKIPFETLNL KVLTKTQPWN NSKRICGVNS FGVGGSNSSL
460 470 480 490 500
FLSSFDKSTT ITEPTTTTTI ESLPSSSSSF DNLSVSSSIS TNNDNDKVSN
510 520 530 540 550
IVNNRYGSSI DVITLSVTSP DKEDLKIRAN DVLESIKTLD DNFKIRDISN
560 570 580 590 600
LTNIRTSHFS NRVAIIGDSI DSIKLNLQSF IKGENNNNKS IILPLINNGN
610 620 630 640 650
NNNNNNNNSS GSSSSSSNNN NICFIFSGQG QQWNKMIFDL YENNKTFKNE
660 670 680 690 700
MNNFSKQFEM ISGWSIIDKL YNSGGGGNEE LINETWLAQP SIVAVQYSLI
710 720 730 740 750
KLFSKDIGIE GSIVLGHSLG ELMAAYYCGI INDFNDLLKL LYIRSTLQNK
760 770 780 790 800
TNGSGRMHVC LSSKAEIEQL ISQLGFNGRI VICGNNTMKS CTISGDNESM
810 820 830 840 850
NQFTKLISSQ QYGSVVHKEV RTNSAFHSHQ MDIIKDEFFK LFNQYFPTNQ
860 870 880 890 900
ISTNQIYDGK SFYSTCYGKY LTPIECKQLL SSPNYWWKNI RESVLFKESI
910 920 930 940 950
EQILQNHQQS LTFIEITCHP ILNYFLSQLL KSSSKSNTLL LSTLSKNSNS
960 970 980 990 1000
IDQLLILCSK LYVNNLSSIK WNWFYDKQQQ QQSESLVSSN FKLPGRRWKL
1010 1020 1030 1040 1050
EKYWIENCQR QMDRIKPPMF ISLDRKLFSV TPSFEVRLNQ DRFQYLNDHQ
1060 1070 1080 1090 1100
IQDIPLVPFS FYIELVYASI FNSISTTTTN TTASTMFEIE NFTIDSSIII
1110 1120 1130 1140 1150
DQKKSTLIGI NFNSDLTKFE IGSINSIGSG SSSNNNFIEN KWKIHSNGII
1160 1170 1180 1190 1200
KYGTNYLKSN SKSNSFNEST TTTTTTTTTT KCFKSFNSNE FYNEIIKYNY
1210 1220 1230 1240 1250
NYKSTFQCVK EFKQFDKQGT FYYSEIQFKK NDKQVIDQLL SKQLPSDFRC
1260 1270 1280 1290 1300
IHPCLLDAVL QSAIIPATNK TNCSWIPIKI GKLSVNIPSN SYFNFKDQLL
1310 1320 1330 1340 1350
YCLIKPSTST STSPSTYFSS DIQVFDKKNN NLICELTNLE FKGINSSSSS
1360 1370 1380 1390 1400
SSSSSTINSN VEANYESKIE ETNHDEDEDE ELPLVSEYVW CKEELINQSI
1410 1420 1430 1440 1450
KFTDNYQTVI FCSTNLNGND LLDSIITSAL ENGHDENKIF IVSPPPVESD
1460 1470 1480 1490 1500
QYNNRIIINY TNNESDFDAL FAIINSTTSI SGKSGLFSTR FIILPNFNSI
1510 1520 1530 1540 1550
TFSSGNSTPL ITNVNGNGNG KSCGGGGGST NNTISNSSSS ISSIDNGNNE
1560 1570 1580 1590 1600
DEEMVLKSFN DSNLSLFHLQ KSIIKNNIKG RLFLITNGGQ SISSSTPTST
1610 1620 1630 1640 1650
YNDQSYVNLS QYQLIGQIRV FSNEYPIMEC SMIDIQDSTR IDLITDQLNS
1660 1670 1680 1690 1700
TKLSKLEIAF RDNIGYSYKL LKPSIFDNSS LPSSSSEIET TATTKDEEKN
1710 1720 1730 1740 1750
NSINYNNNYY RVELSDNGII SDLKIKQFRQ MKCGVGQVLV RVEMCTLNFR
1760 1770 1780 1790 1800
DILKSLGRDY DPIHLNSMGD EFSGKVIEIG EGVNNLSVGQ YVFGINMSKS
1810 1820 1830 1840 1850
MGSFVCCNSD LVFPIPIPTP SSSSSSNENI DDQEIISKLL NQYCTIPIVF
1860 1870 1880 1890 1900
LTSWYSIVIQ GRLKKGEKIL IHSGCGGVGL ATIQISMMIG AEIHVTVGSN
1910 1920 1930 1940 1950
EKKQYLIKEF GIDEKRIYSS RSLQFYNDLM VNTDGQGVDM VLNSLSGEYL
1960 1970 1980 1990 2000
EKSIQCLSQY GRFIEIGKKD IYSNSSIHLE PFKNNLSFFA VDIAQMTENR
2010 2020 2030 2040 2050
RDYLREIMID QLLPCFKNGS LKPLNQHCFN SPCDLVKAIR FMSSGNHIGK
2060 2070 2080 2090 2100
ILINWSNLNN DKQFINHHSV VHLPIQSFSN RSTYIFTGFG GLTQTLLKYF
2110 2120 2130 2140 2150
STESDLTNVI IVSKNGLDDN SGSGSGNNEK LKLINQLKES GLNVLVEKCD
2160 2170 2180 2190 2200
LSSIKQVYKL FNKIFDNDAS GSDSGDFSDI KGIFHFASLI NDKRILKHNL
2210 2220 2230 2240 2250
ESFNYVYNSK ATSAWNLHQV SLKYNLNLDH FQTIGSVITI LGNIGQSNYT
2260 2270 2280 2290 2300
CANRFVEGLT HLRIGMGLKS SCIHLASIPD VGMASNDNVL NDLNSMGFVP
2310 2320 2330 2340 2350
FQSLNEMNLG FKKLLSSPNP IVVLGEINVD RFIEATPNFR AKDNFIITSL
2360 2370 2380 2390 2400
FNRIDPLLLV NESQDFIINN NINNNGGGGD GSFDDLNQLE DEGQQGFGNG
2410 2420 2430 2440 2450
DGYVDDNIDS VSMLSGTSSI FDNDFYTKSI RGMLCDILEL KDKDLNNTVS
2460 2470 2480 2490 2500
FSDYGLDSLL SSELSNTIQK NFSILIPSLT LVDNSTINST VELIKNKLKN
2510 2520 2530 2540 2550
STTSSISSSV SKKVSFKKNT QPLIIPTTAP ISIIKTQSYI KSEIIESLPI
2560 2570 2580 2590 2600
SSSTTIKPLV FDNLVYSSSS SNNSNSKNEL TSPPPSAKRE SVLPIISEDN
2610 2620 2630 2640 2650
NSDNDSSMAT VIYEISPIAA PYHRYQTDVL KEITQLTPHK EFIDNIYKKS
2660 2670 2680 2690 2700
KIRSRYCFND FSEKSMADIN KLDAGERVAL FREQTYQTVI NAGKTVIERA
2710 2720 2730 2740 2750
GIDPMLISHV VGVTSTGIMA PSFDVVLIDK LGLSINTSRT MINFMGCGAA
2760 2770 2780 2790 2800
VNSMRAATAY AKLKPGTFVL VVAVEASATC MKFNFDSRSD LLSQAIFTDG
2810 2820 2830 2840 2850
CVATLVTCQP KSSLVGKLEI IDDLSYLMPD SRDALNLFIG PTGIDLDLRP
2860 2870 2880 2890 2900
ELPIAINRHI NSAITSWLKK NSLQKSDIEF FATHPGGAKI ISAVHEGLGL
2910 2920 2930 2940 2950
SPEDLSDSYE VMKRYGNMIG VSTYYVLRRI LDKNQTLLQE GSLGYNYGMA
2960
MAFSPGASIE AILFKLIK
Length:2,968
Mass (Da):329,909
Last modified:May 24, 2005 - v1
Checksum:i98AA21F6256A8A57
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000171 Genomic DNA. Translation: EAL62021.1.
RefSeqiXP_635518.1. XM_630426.1.

Genome annotation databases

EnsemblProtistsiDDB0234163; DDB0234163; DDB_G0290853.
GeneIDi8627855.
KEGGiddi:DDB_G0290853.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000171 Genomic DNA. Translation: EAL62021.1 .
RefSeqi XP_635518.1. XM_630426.1.

3D structure databases

ProteinModelPortali Q54FI3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0219613.

Proteomic databases

PRIDEi Q54FI3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0234163 ; DDB0234163 ; DDB_G0290853 .
GeneIDi 8627855.
KEGGi ddi:DDB_G0290853.

Organism-specific databases

dictyBasei DDB_G0290853. stlB.

Phylogenomic databases

eggNOGi COG3424.
InParanoidi Q54FI3.
OMAi HINSAIT.
PhylomeDBi Q54FI3.

Enzyme and pathway databases

UniPathwayi UPA00154 .

Family and domain databases

Gene3Di 1.10.1200.10. 1 hit.
3.40.366.10. 2 hits.
3.40.47.10. 4 hits.
3.40.50.720. 2 hits.
3.90.180.10. 1 hit.
InterProi IPR001227. Ac_transferase_dom.
IPR009081. Acyl_carrier_prot-like.
IPR014043. Acyl_transferase.
IPR016035. Acyl_Trfase/lysoPLipase.
IPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR012328. Chalcone/stilbene_synth_C.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011032. GroES-like.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
IPR013968. PKS_KR.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00698. Acyl_transf_1. 1 hit.
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
PF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
PF08659. KR. 1 hit.
PF00550. PP-binding. 1 hit.
[Graphical view ]
SMARTi SM00829. PKS_ER. 1 hit.
[Graphical view ]
SUPFAMi SSF47336. SSF47336. 1 hit.
SSF50129. SSF50129. 2 hits.
SSF52151. SSF52151. 2 hits.
SSF53901. SSF53901. 4 hits.
PROSITEi PS50075. ACP_DOMAIN. 1 hit.
PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Biosynthesis of Dictyostelium discoideum differentiation-inducing factor by a hybrid type I fatty acid-type III polyketide synthase."
    Austin M.B., Saito T., Bowman M.E., Haydock S., Kato A., Moore B.S., Kay R.R., Noel J.P.
    Nat. Chem. Biol. 2:494-502(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  3. "Polyketide synthase genes and the natural products potential of Dictyostelium discoideum."
    Zucko J., Skunca N., Curk T., Zupan B., Long P.F., Cullum J., Kessin R.H., Hranueli D.
    Bioinformatics 23:2543-2549(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiPKS37_DICDI
AccessioniPrimary (citable) accession number: Q54FI3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 24, 2005
Last modified: November 26, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Encoded by one of the numerous copies of polyketide synthase genes localized in chromosome 5.
In reference to their hybrid nature and to their discovery in D.discoideum, authors term these type I FAS-type III PKS fusion enzymes 'steely'.1 Publication

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3