Reviewed, UniProtKB/Swiss-Prot Q54FB7 (XDH_DICDI)

Names and origin

Protein names

Recommended name:
    Xanthine dehydrogenase
      Short name=XD
    EC=1.17.1.4

Gene names

Name:	xdh
ORF Names:	DDB_G0291047

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Xanthine + NAD+ + H2O = urate + NADH. Hypoxanthine + NAD+ + H2O = xanthine + NADH.
Cofactor	Binds 2 2Fe-2S clusters By similarity. FAD By similarity. Molybdopterin By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Peroxisome By similarity.
Sequence similarities	Belongs to the xanthine dehydrogenase family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Cellular component	Peroxisome
Ligand	2Fe-2S FAD Flavoprotein Iron Iron-sulfur Metal-binding Molybdenum NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW xanthine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1358	1358	Xanthine dehydrogenase		PRO_0000327650
Regions
	Domain	18 – 107	90	2Fe-2S ferredoxin-type
	Domain	253 – 447	195	FAD-binding PCMH-type
Sites
	Metal binding	56	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	61	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	64	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54FB7-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: AF9AEE1059FE076F	FASTA	1,358	149,928
	10 20 30 40 50 60 MIDDENLNGK EPFLKKENQL LFFLNGEKVL INEPNPELST LDYIRSIGLT GLKRGCSEGA 70 80 90 100 110 120 CGSCTFMLSN VVKDDNDTFR IVHRAVNGCL YPLCALDGMA VTTIEGLGNI DKGLHSIQER 130 140 150 160 170 180 ISENSGSQCG FCTPGIIMAL YAFLRSNPNS TQKDIEQNFD GNLCRCTGYR PILDAAKSFA 190 200 210 220 230 240 NQPSDEQLVE LPLPPMATID DKKDDTQMIC PGTGKPCNCK TKTSHIPNKP MELNSEPIFP 250 260 270 280 290 300 PFLMEYKKES LKFTGSRVTW YTPTTLEELL KIKKEKTNAK IVVGNTEIGI ETRFRSIVYP 310 320 330 340 350 360 TIICPTRVEE LIQIQKEDNG VRVGASVTLT EMKSYLNGII KSSENDEIAN KKNGTFKAII 370 380 390 400 410 420 SQLKWFAGNQ VRNAASIGGN LCTASPISDL NPVLLAAGAV LTMVSLDDNG AKVRRQVPIN 430 440 450 460 470 480 QFFLRYRVVD IKPEEILESV FIPYTRPLEF IQAYKQSRRR EDDIAIVSCC FRVLLEPIAE 490 500 510 520 530 540 SASNTVDSNF KIKDCVLAYG GMNVKAVTCE KTEKQLIGSV WSRELLNDAC LNLESDLPLA 550 560 570 580 590 600 AGAPGGMIEY RRSLTTGFFF KYFLTVSKQL YQISNGNPLY LVSDKEKSAT DAYSRPLSFG 610 620 630 640 650 660 EQNYQTQPDK HPITQPIKHQ SADKQVTGEA LYVDDVKMKS LYAVMVPSLK AHANIKSVDA 670 680 690 700 710 720 SKALKAPGVK AFFSAKDIPG INDCGPVIHD EEVFVTKTAL FHGAPIGCIV AETHIQALEA 730 740 750 760 770 780 SKLVAIEYEE LPAITSIEDA ISKQSFFPFT HLLKDGDMEK GWSESDHIID GEFKVGAQEH 790 800 810 820 830 840 FYLEPNGTLV IPGEGKELTV ISSTQNPTKT QAIVASVLGI GQNQVVCKLK RLGGGFGGKE 850 860 870 880 890 900 TRSIFSSCVA AIASYHMKEP VRIILDRDTD MSTTGTRHPF IARYRVGFTK EGLIKALDLE 910 920 930 940 950 960 LYADAGFSYD ISVGVLDRAI FHSENSYKIP NVNILGRLCK TNLPSNTAFR GYGGPQAMII 970 980 990 1000 1010 1020 CENWVEKISK TLGMDSYKIR ELNFYKEAEV TAYRQSVVNN MMKRVWDELM VKSNYHQRLI 1030 1040 1050 1060 1070 1080 AVEKFNKENR YKKRGISIIP TKFGMSFTVK TLNQAGALVH VYTDGTILVT HGGTEMGQGL 1090 1100 1110 1120 1130 1140 NTKMIQIAAR AFNVPVSDVF ISETSTDKVP NTAPTAASVS SDLNGMAVLD ACQQILLRME 1150 1160 1170 1180 1190 1200 PIREKNPNVP FKQLCTLCFV ERVNLSANGF YATPNVGYMF KDSGVGEGTP FNYFNFGAAC 1210 1220 1230 1240 1250 1260 SEVEIDTLTG DHTTLRSDVI LDVGDSLNPT IDIGQVEGAF VQGMGWSTLE EVVTFPSGYM 1270 1280 1290 1300 1310 1320 FTRGPSTYKI PGFNDVPIEF NVSLLGDAPN PKAIHSSKGV GEPPLFLGSS VYFAIRQAIT 1330 1340 1350 AARLENNLTN WFDLQSPATC ERIRTSCLDN FVLQFRKQ

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000174 Genomic DNA. Translation: EAL61954.1.
RefSeq	XP_635420.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3387033.
KEGG	ddi:DDB_0230176.
Organism-specific databases
dictyBase	DDB_G0291047. xdh.
Phylogenomic databases
OMA	Q54FB7. LLFQKDP.
Enzyme and pathway databases
BRENDA	1.17.1.4. 424.
Family and domain databases
InterPro	IPR002888. 2Fe-2S_bd. IPR006058. 2Fe2S_fd_BS. IPR000674. Ald_Oxase/Xan_DH_a/b. IPR016208. Ald_Oxase/xanthine_DH. IPR008274. AldOxase/xan_DH_Mopterin-bd. IPR005107. CO_DH_flav_C. IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR001041. Ferredoxin. IPR002346. Mopterin_DH_FAD-bd. IPR000572. OxRdtase_Mopterin-bd. [Graphical view]
Gene3D	G3DSA:3.30.365.10. Ald_xan_DH_mo_bd. 2 hits. G3DSA:3.90.1170.50. Aldxan_DH_hamm. 1 hit. G3DSA:3.30.390.50. CO_DH_flav_C. 1 hit. G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit.
Pfam	PF01315. Ald_Xan_dh_C. 1 hit. PF02738. Ald_Xan_dh_C2. 1 hit. PF03450. CO_deh_flav_C. 1 hit. PF00941. FAD_binding_5. 1 hit. PF00111. Fer2. 1 hit. PF01799. Fer2_2. 1 hit. [Graphical view]
PIRSF	PIRSF000127. Xanthine_DH. 1 hit.
ProDom	PD186071. 2Fe-2S_bind. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51387. FAD_PCMH. 1 hit. PS00559. MOLYBDOPTERIN_EUK. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XDH_DICDI

Accession

Primary (citable) accession number: Q54FB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)