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Q54FB7 (XDH_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Xanthine dehydrogenase

Short name=XD
EC=1.17.1.4
Gene names
Name:xdh
ORF Names:DDB_G0291047
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1358 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid By similarity.

Catalytic activity

Xanthine + NAD+ + H2O = urate + NADH.

Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactor

Binds 2 2Fe-2S clusters By similarity.

FAD By similarity.

Binds 1 molybdenum-molybdopterin (Mo-MPT) cofactor per subunit By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Peroxisome By similarity.

Sequence similarities

Belongs to the xanthine dehydrogenase family.

Contains 1 2Fe-2S ferredoxin-type domain.

Contains 1 FAD-binding PCMH-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13581358Xanthine dehydrogenase
PRO_0000327650

Regions

Domain18 – 107902Fe-2S ferredoxin-type
Domain253 – 447195FAD-binding PCMH-type
Nucleotide binding281 – 2888FAD By similarity
Nucleotide binding376 – 3805FAD By similarity

Sites

Active site13021Proton acceptor By similarity
Metal binding561Iron-sulfur 1 By similarity
Metal binding611Iron-sulfur 1 By similarity
Metal binding641Iron-sulfur 1 By similarity
Metal binding891Iron-sulfur 1 By similarity
Metal binding1291Iron-sulfur 2 By similarity
Metal binding1321Iron-sulfur 2 By similarity
Metal binding1641Iron-sulfur 2 By similarity
Metal binding1661Iron-sulfur 2 By similarity
Metal binding8051Molybdenum By similarity
Metal binding8361Molybdenum; via carbonyl oxygen By similarity
Metal binding9501Molybdenum; via amide nitrogen By similarity
Metal binding11171Molybdenum; via amide nitrogen By similarity
Binding site3661FAD By similarity
Binding site3891FAD By similarity
Binding site4371FAD; via amide nitrogen and carbonyl oxygen By similarity
Binding site4551FAD By similarity
Binding site8401Substrate By similarity
Binding site9181Substrate By similarity
Binding site9521Substrate By similarity
Binding site10481Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54FB7 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: AF9AEE1059FE076F

FASTA1,358149,928
        10         20         30         40         50         60 
MIDDENLNGK EPFLKKENQL LFFLNGEKVL INEPNPELST LDYIRSIGLT GLKRGCSEGA 

        70         80         90        100        110        120 
CGSCTFMLSN VVKDDNDTFR IVHRAVNGCL YPLCALDGMA VTTIEGLGNI DKGLHSIQER 

       130        140        150        160        170        180 
ISENSGSQCG FCTPGIIMAL YAFLRSNPNS TQKDIEQNFD GNLCRCTGYR PILDAAKSFA 

       190        200        210        220        230        240 
NQPSDEQLVE LPLPPMATID DKKDDTQMIC PGTGKPCNCK TKTSHIPNKP MELNSEPIFP 

       250        260        270        280        290        300 
PFLMEYKKES LKFTGSRVTW YTPTTLEELL KIKKEKTNAK IVVGNTEIGI ETRFRSIVYP 

       310        320        330        340        350        360 
TIICPTRVEE LIQIQKEDNG VRVGASVTLT EMKSYLNGII KSSENDEIAN KKNGTFKAII 

       370        380        390        400        410        420 
SQLKWFAGNQ VRNAASIGGN LCTASPISDL NPVLLAAGAV LTMVSLDDNG AKVRRQVPIN 

       430        440        450        460        470        480 
QFFLRYRVVD IKPEEILESV FIPYTRPLEF IQAYKQSRRR EDDIAIVSCC FRVLLEPIAE 

       490        500        510        520        530        540 
SASNTVDSNF KIKDCVLAYG GMNVKAVTCE KTEKQLIGSV WSRELLNDAC LNLESDLPLA 

       550        560        570        580        590        600 
AGAPGGMIEY RRSLTTGFFF KYFLTVSKQL YQISNGNPLY LVSDKEKSAT DAYSRPLSFG 

       610        620        630        640        650        660 
EQNYQTQPDK HPITQPIKHQ SADKQVTGEA LYVDDVKMKS LYAVMVPSLK AHANIKSVDA 

       670        680        690        700        710        720 
SKALKAPGVK AFFSAKDIPG INDCGPVIHD EEVFVTKTAL FHGAPIGCIV AETHIQALEA 

       730        740        750        760        770        780 
SKLVAIEYEE LPAITSIEDA ISKQSFFPFT HLLKDGDMEK GWSESDHIID GEFKVGAQEH 

       790        800        810        820        830        840 
FYLEPNGTLV IPGEGKELTV ISSTQNPTKT QAIVASVLGI GQNQVVCKLK RLGGGFGGKE 

       850        860        870        880        890        900 
TRSIFSSCVA AIASYHMKEP VRIILDRDTD MSTTGTRHPF IARYRVGFTK EGLIKALDLE 

       910        920        930        940        950        960 
LYADAGFSYD ISVGVLDRAI FHSENSYKIP NVNILGRLCK TNLPSNTAFR GYGGPQAMII 

       970        980        990       1000       1010       1020 
CENWVEKISK TLGMDSYKIR ELNFYKEAEV TAYRQSVVNN MMKRVWDELM VKSNYHQRLI 

      1030       1040       1050       1060       1070       1080 
AVEKFNKENR YKKRGISIIP TKFGMSFTVK TLNQAGALVH VYTDGTILVT HGGTEMGQGL 

      1090       1100       1110       1120       1130       1140 
NTKMIQIAAR AFNVPVSDVF ISETSTDKVP NTAPTAASVS SDLNGMAVLD ACQQILLRME 

      1150       1160       1170       1180       1190       1200 
PIREKNPNVP FKQLCTLCFV ERVNLSANGF YATPNVGYMF KDSGVGEGTP FNYFNFGAAC 

      1210       1220       1230       1240       1250       1260 
SEVEIDTLTG DHTTLRSDVI LDVGDSLNPT IDIGQVEGAF VQGMGWSTLE EVVTFPSGYM 

      1270       1280       1290       1300       1310       1320 
FTRGPSTYKI PGFNDVPIEF NVSLLGDAPN PKAIHSSKGV GEPPLFLGSS VYFAIRQAIT 

      1330       1340       1350 
AARLENNLTN WFDLQSPATC ERIRTSCLDN FVLQFRKQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000174 Genomic DNA. Translation: EAL61954.1.

3D structure databases

ProteinModelPortalQ54FB7.
SMRQ54FB7. Positions 18-181.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0230176.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0230176; DDB0230176; DDB_G0291047.
KEGGddi:DDB_G0291047.

Organism-specific databases

dictyBaseDDB_G0291047. xdh.

Phylogenomic databases

eggNOGCOG4630.
KOK00106.
OMAFHMENSY.
PhylomeDBQ54FB7.
ProtClustDBCLSZ2429618.

Family and domain databases

Gene3D1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
[Graphical view]
PfamPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFPIRSF000127. Xanthine_DH. 1 hit.
SMARTSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
PROSITEPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54FB7.

Entry information

Entry nameXDH_DICDI
AccessionPrimary (citable) accession number: Q54FB7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase