Q54FB6 (DAD1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
Short name=Oligosaccharyl transferase subunit 2
EC=2.4.1.119

Gene names

Name:	ost2
ORF Names:	DDB_G0291049

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the DAD/OST2 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein N-linked glycosylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 120	120	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2		PRO_0000327672
Regions
	Transmembrane	37 – 57	21	Helical; Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Transmembrane	100 – 120	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54FB6 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 7ED4F91B7E2E801A	FASTA	120	13,471
	10 20 30 40 50 60 MSTTASTSSN NLTFTSIVKS FFESYSKTPQ KLKIIDLFLI YTFITGVIVF TYCCLVGTYP 70 80 90 100 110 120 FNSFLAAFIS TVGCFVLTVC LRIQINPINN FGKTISIERA FTDYLLCNLI LHLVVFNFLG

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000174 Genomic DNA. Translation: EAL61955.1.
RefSeq	XP_635421.1. XM_630329.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54FB6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233175; DDB0233175; DDB_G0291049.
GeneID	8627922.
GenomeReviews	Gene locus ost2 in contig CM000154_GR.
KEGG	ddi:DDB_G0291049.
Organism-specific databases
dictyBase	DDB_G0291049. ost2.
Phylogenomic databases
eggNOG	KOG1746.
HOGENOM	HBG329225.
OMA	YCCLVGT.
PhylomeDB	Q54FB6.
Family and domain databases
InterPro	IPR003038. DAD. [Graphical view]
KO	K12668.
PANTHER	PTHR10705. DAD. 1 hit.
Pfam	PF02109. DAD. 1 hit. [Graphical view]
PIRSF	PIRSF005588. DAD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DAD1_DICDI

Accession

Primary (citable) accession number: Q54FB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)