Reviewed, UniProtKB/Swiss-Prot Q54F93 (MPPA_DICDI)

Names and origin

Protein names

Recommended name:
    Mitochondrial-processing peptidase subunit alpha
    EC=3.4.24.64
Alternative name(s):
    Alpha-MPP

Gene names

Name:	pmpca
ORF Names:	DDB_G0290997

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The mitochondrial processing protease (MPP-I) cleaves presequences from mitochondrial protein precursors. Most MPP-I cleavage sites follow an arginine at position -2.
Catalytic activity	Release of N-terminal transit peptides from precursor proteins imported into the mitochondrion, typically with Arg in position P2.
Subunit structure	Heterodimer of alpha and beta subunits.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the peptidase M16 family.
Caution	Does not seem to have a protease activity as it lack the zinc-binding site.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell phagocytic vesicle Ref.2 Inferred from direct assay. Source: dictyBase
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion
	Chain	? – 445		Mitochondrial-processing peptidase subunit alpha		PRO_0000337763

Sequences

	Sequence		Length	Mass (Da)
	Q54F93-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: C8D40AF48528740B	FASTA	445	47,746
	10 20 30 40 50 60 MIGRFIARNY TTSIFQESKR IVESTTLSNG LKVVSLVGGY TGPAVSLGLY IKTGSRNETQ 70 80 90 100 110 120 ETAGLNQVLK GLAFESNTNK LGIEVQRDIE VSGSTAFAQA SRDNLLIALQ TLPNRSLQML 130 140 150 160 170 180 NNLANITKPT LPYHEVRDVT EIIVKESEAY NHDSYSSIFE SVHQTAFRGK TLGRPLVAPI 190 200 210 220 230 240 CNLGNITKDA VTNWVNSTYK PSNMILVGVG LSHNELIEEA EKVTFGNDES STSISNETAQ 250 260 270 280 290 300 YIGGESLKYS SGNSKVVLAF EGTAQSNIKD VAAFSVLQSI LGNGCPKTAP GHGRTSRLFS 310 320 330 340 350 360 LTKNNSNIVN SEAFNLTYGD SGLFGVVAEV EGATVGKTVS LITSEIVAAS KTAGQELERA 370 380 390 400 410 420 KAVTKSSVLE QAESRTSALE FIGKQAIYTD KVLTPAEFAE EISKVTSEDI KRVAKKMTSK 430 440 KPTLVVVGDV SDAPTIESVQ SQLKL

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

[2]

"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000174 Genomic DNA. Translation: EAL61929.1.
RefSeq	XP_635444.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3387002.
KEGG	ddi:DDB_0237967.
Organism-specific databases
dictyBase	DDB_G0290997.
Phylogenomic databases
OMA	Q54F93. LEACCED.
Family and domain databases
InterPro	IPR011237. Pept_M16_core. IPR011765. Pept_M16_N. IPR007863. Peptidase_M16_C. [Graphical view]
Gene3D	G3DSA:3.30.830.10. Pept_M16_core. 2 hits.
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPPA_DICDI

Accession

Primary (citable) accession number: Q54F93

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)