Q54F73 (CCS_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable copper chaperone for superoxide dismutase

Alternative name(s):
Superoxide dismutase copper chaperone

Gene names

Name:	ccs
Synonyms:	ccsA
ORF Names:	DDB_G0291031

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Delivers copper to copper zinc superoxide dismutases By similarity.
Cofactor	Binds 2 copper ions per subunit By similarity.
Subunit structure	Homodimer, and heterodimer with SOD1 By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the C-terminal section; belongs to the Cu-Zn superoxide dismutase family. Contains 1 HMA domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Copper Metal-binding
Molecular function	Chaperone
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular copper ion transport Inferred from sequence or structural similarity. Source: dictyBase removal of superoxide radicals Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: dictyBase mitochondrial inner membrane Inferred from sequence or structural similarity. Source: dictyBase nucleus Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	superoxide dismutase activity Inferred from Biological aspect of Ancestor. Source: RefGenome superoxide dismutase copper chaperone activity Inferred from sequence or structural similarity. Source: dictyBase zinc ion binding Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Probable copper chaperone for superoxide dismutase		PRO_0000329400
Regions
	Domain	8 – 72	65	HMA
	Region	99 – 298	200	Superoxide dismutase-like
	Compositional bias	78 – 84	7	Poly-Thr
	Compositional bias	182 – 210	29	Poly-Asn
	Compositional bias	268 – 275	8	Poly-Asn
Sites
	Metal binding	18	1	Copper 1 Potential
	Metal binding	21	1	Copper 1 Potential
	Metal binding	308	1	Copper 2 By similarity
	Metal binding	310	1	Copper 2 By similarity
Amino acid modifications
	Disulfide bond	169 ↔ 291		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54F73 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: C167C77135B10589	FASTA	316	34,468
	10 20 30 40 50 60 MDDKNLEIKV ELNVDISCQS CVDSISKELR EKLENTKLVE HDIPEQRIVL QGTDLTQDIL 70 80 90 100 110 120 ETIKNTGRNA TICGLSSTTT TTSTSPSSST CHKKQETVSG SAVCSLGLIE NWQKGCGGSG 130 140 150 160 170 180 GVGSKGVYGV IRLLRASTTK TLFEGRITGL KPGKHSLVVH EFGNLMNGCD DLGEPFISNV 190 200 210 220 230 240 ENNNNNNNNN NNNNNNNNNN NNNNNNNNKN INKCKEILNK IIGTSDVKQD GKAEFRVLSD 250 260 270 280 290 300 KYDFWDLIGR SIVLHSQDSK YSPIEDLNNN NNNKNIVNSE SDKILGERVA CGIISRAASI 310 GQNHKKVCPC DGTTAI

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000174 Genomic DNA. Translation: EAL61946.1.
RefSeq	XP_635464.1. XM_630372.1.
3D structure databases
HSSP	HSSP built from PDB template 1OZU based on UniProtKB P00441.
ProteinModelPortal	Q54F73.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0238038; DDB0238038; DDB_G0291031.
GeneID	8627964.
KEGG	ddi:DDB_G0291031.
Organism-specific databases
dictyBase	DDB_G0291031. ccs.
Phylogenomic databases
eggNOG	NOG327209.
OMA	KTLFEGR.
Family and domain databases
Gene3D	2.60.40.200. 2 hits.
InterPro	IPR006121. HeavyMe-assoc_HMA. IPR024134. SOD_Cu/Zn_/chaperones. IPR024142. SOD_Cu_chaperone. IPR001424. SOD_Cu_Zn_dom. [Graphical view]
PANTHER	PTHR10003. PTHR10003. 1 hit. PTHR10003:SF27. PTHR10003:SF27. 1 hit.
Pfam	PF00080. Sod_Cu. 1 hit. [Graphical view]
SUPFAM	SSF55008. HeavyMe_transpt. 1 hit. SSF49329. SOD_Cu_Zn. 1 hit.
PROSITE	PS01047. HMA_1. False negative. PS50846. HMA_2. False negative. PS00087. SOD_CU_ZN_1. False negative. PS00332. SOD_CU_ZN_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCS_DICDI

Accession

Primary (citable) accession number: Q54F73

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)