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Protein

Superoxide-generating NADPH oxidase heavy chain subunit C

Gene

noxC

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Critical component of the membrane-bound oxidase that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior (By similarity).By similarity

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi665Iron (heme axial ligand)By similarity1
Metal bindingi679Iron (heme axial ligand)By similarity1
Metal bindingi755Iron (heme axial ligand)By similarity1
Metal bindingi768Iron (heme axial ligand)By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi519 – 530PROSITE-ProRule annotationAdd BLAST12
Nucleotide bindingi849 – 855FADSequence analysis7

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • flavin adenine dinucleotide binding Source: dictyBase
  • heme binding Source: dictyBase
  • superoxide-generating NADPH oxidase activity Source: dictyBase

GO - Biological processi

  • defense response to bacterium Source: dictyBase
  • sorocarp development Source: dictyBase
  • sporulation resulting in formation of a cellular spore Source: dictyBase
  • superoxide anion generation Source: dictyBase

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandCalcium, FAD, Flavoprotein, Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

ReactomeiR-DDI-3299685. Detoxification of Reactive Oxygen Species.

Names & Taxonomyi

Protein namesi
Recommended name:
Superoxide-generating NADPH oxidase heavy chain subunit C (EC:1.-.-.-)
Alternative name(s):
NADPH oxidase C
Superoxide-generating NADPH oxidase flavocytochrome C
Gene namesi
Name:noxC
ORF Names:DDB_G0291117
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 5, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291117. noxC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei583 – 603HelicalSequence analysisAdd BLAST21
Transmembranei627 – 647HelicalSequence analysisAdd BLAST21
Transmembranei667 – 687HelicalSequence analysisAdd BLAST21
Transmembranei720 – 740HelicalSequence analysisAdd BLAST21
Transmembranei754 – 774HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: dictyBase
  • NADPH oxidase complex Source: dictyBase

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Prevents formation of fruiting bodies.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003615291 – 1142Superoxide-generating NADPH oxidase heavy chain subunit CAdd BLAST1142

Proteomic databases

PaxDbiQ54F44.

Expressioni

Developmental stagei

Expressed during development. Low expression starts at 8 hours development, and increases sharply in fruiting bodies.1 Publication

Interactioni

Subunit structurei

Composed of a heavy chain and a light chain.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0191391.

Structurei

3D structure databases

ProteinModelPortaliQ54F44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini462 – 497EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini506 – 541EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini622 – 766Ferric oxidoreductaseAdd BLAST145
Domaini794 – 942FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST149

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili172 – 246Sequence analysisAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi186 – 189Poly-Lys4
Compositional biasi211 – 231Poly-AsnAdd BLAST21
Compositional biasi234 – 273Poly-AsnAdd BLAST40
Compositional biasi336 – 341Poly-Ser6
Compositional biasi885 – 891Poly-Gln7
Compositional biasi911 – 915Poly-Asn5

Keywords - Domaini

Coiled coil, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
InParanoidiQ54F44.
OMAiYMNYSQA.
PhylomeDBiQ54F44.

Family and domain databases

CDDicd00051. EFh. 1 hit.
InterProiView protein in InterPro
IPR000778. Cyt_b245_heavy_chain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
PfamiView protein in Pfam
PF13499. EF-hand_7. 1 hit.
PF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
PRINTSiPR00466. GP91PHOX.
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF63380. SSF63380. 2 hits.
PROSITEiView protein in PROSITE
PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS51384. FAD_FR. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54F44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKQKNISIK KFANPLSFPH EFRTNLHKST FLDASLLELP NISVDKTKNE
60 70 80 90 100
NNFSITDKIL MESLKENEFK IREFIKNQES GNRVLMIDKN SLKELFNIND
110 120 130 140 150
NQALDLIFNQ YVQSTRPNLI RKSSNSSLNI DLKKEIKKKT KKSRKSFSRN
160 170 180 190 200
SKLNNNDSKI DDKNDNYIDK INDINNFNSD IEEIFKKKKE IENTLKIPIG
210 220 230 240 250
GNSFPIPVEF NNNNNNNNNN NNNNNNNYNN NIDNNNNNNN NNNNNNINNC
260 270 280 290 300
NNNNINNDNN NNNNNDNDNN NINTVDNHDD DIINNSNNFN KNEYPSSNIS
310 320 330 340 350
PISPKSSISS FPTNLNNSIN NTGSMVSDSL SSCRNSISSS SIDSSVASIP
360 370 380 390 400
ITIQSIDFED KNIKSDQFKI ISKSNIENTI ETNPIPPFNQ TNNQCEVQLQ
410 420 430 440 450
SHSLPTILKQ PHIYKSKSFS SSINSNSKIK KIKKSRSFEI ESKINLFDVI
460 470 480 490 500
NHIYLNLSKV GSEEQKITSV FKLYDIYDKG FISRDDLKEV LNYRTKQNGL
510 520 530 540 550
KFQDFTMESL IDHIFQQFDK NMDGYIDFEE FKSELTINNE NKVKEKEENT
560 570 580 590 600
NYNFKEENIG IYTEKESFHS LKRYLKIEGS KLFFISLFFI INSILVITSF
610 620 630 640 650
LNVHANNKRA IELFGPGVYI TRIAAQLIEF NAAIILMTMC KQLFTMIRNT
660 670 680 690 700
KFKFLFPVDK YMTFHKLIGY TLIIASFLHT IGWIVGMAVA TGKPDNIFYD
710 720 730 740 750
CLAPHFKFRP TVWEMIFNSL PGVTGFIMIS FLIIMAILSL KIIRKSNFEL
760 770 780 790 800
FYYSHHLFIG FYVLLILHGT MGWIRPPTFW KWFIVPGFFY TVDRSFRLFK
810 820 830 840 850
RTHRVEVLDY CLKNERVINL TFSKPPSFDY KPGQYLLINV PHISKLQWHP
860 870 880 890 900
FTMTSSPLED KIYVHIRVTG NWTKKLFRWL SIKKQLQQQQ QLYNNIKQQN
910 920 930 940 950
VLPDGSNFII NNNNNIDQID LEIGLKPFRI NIDGPFGSSS QYALKQKQVI
960 970 980 990 1000
LVGAGIGVSP MASLLKDISL KKQRLQLLNQ GDQIALEQSK NEITTKFGLG
1010 1020 1030 1040 1050
NLEKVHFFWL NRDQHSFQWF EDLLIDISTN GNSNLPKISI NTFNTRVFPK
1060 1070 1080 1090 1100
NDVRVFMLWN GLDKLFKAQG LDPTTNLPFK THWGRPNWDT IFQYYSKKYS
1110 1120 1130 1140
GESISVFCCG PSQLSKELYE KCRYYTCLKT GGTKFYFHKE NF
Length:1,142
Mass (Da):132,499
Last modified:May 24, 2005 - v1
Checksum:iEE780670085977A9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224 – 225NN → II in AAO72635 (PubMed:15950752).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY224390 Genomic DNA. Translation: AAO72635.1.
AAFI02000175 Genomic DNA. Translation: EAL61883.1.
RefSeqiXP_635387.1. XM_630295.1.

Genome annotation databases

EnsemblProtistsiEAL61883; EAL61883; DDB_G0291117.
GeneIDi8627994.
KEGGiddi:DDB_G0291117.

Entry informationi

Entry nameiNOXC_DICDI
AccessioniPrimary (citable) accession number: Q54F44
Secondary accession number(s): Q86MX0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: May 24, 2005
Last modified: June 7, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase