Q54F10 (NDUV2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
EC=1.6.5.3
EC=1.6.99.3

Gene names

Name:	ndufv2
ORF Names:	DDB_G0291173

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster Potential.
Subunit structure	Complex I is composed of 45 different subunits. This is a component of the flavoprotein-sulfur (FP) fragment of the enzyme By similarity.
Subcellular location	Mitochondrion inner membrane By similarity.
Sequence similarities	Belongs to the complex I 24 kDa subunit family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, NADH to ubiquinone Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	mitochondrial respiratory chain complex I Inferred from Biological aspect of Ancestor. Source: RefGenome
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from Biological aspect of Ancestor. Source: RefGenome electron carrier activity Inferred from sequence or structural similarity. Source: dictyBase metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Mitochondrion Potential
	Chain	41 – 247	207	NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial		PRO_0000328010
Sites
	Metal binding	135	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	140	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	176	1	Iron-sulfur (2Fe-2S) Potential
	Metal binding	180	1	Iron-sulfur (2Fe-2S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54F10 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: DE27EC529EAC9760	FASTA	247	27,742
	10 20 30 40 50 60 MFRSLLKRTT FLNQLNKSNG FNRNYFKQST LTRSDALSRH VETEDNNEHT PFDFTQENLV 70 80 90 100 110 120 KVEKILAKYP KQYRQSALIP LLDLAQRQNG GWISLRAMDK VAHICGIAPM TAYEVASFYT 130 140 150 160 170 180 MFNRTKIGEN FVQVCTTTPC MLRGSGEIIK TCKSHLGIQV GETTPDNKFT LVEVECLGAC 190 200 210 220 230 240 VNAPMMCIND DFYEDLTSAS TINLLDQIKN NKPTKIGPQT HRKAAEGPQG KTTLLEPPVG PTCRDDL

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000175 Genomic DNA. Translation: EAL61870.1.
RefSeq	XP_635380.1. XM_630288.1.
3D structure databases
ProteinModelPortal	Q54F10.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0233206.
Proteomic databases
PRIDE	Q54F10.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233206; DDB0233206; DDB_G0291173.
GeneID	8628028.
KEGG	ddi:DDB_G0291173.
Organism-specific databases
dictyBase	DDB_G0291173. ndufv2.
Phylogenomic databases
eggNOG	COG1905.
KO	K03943.
OMA	SMAYIRV.
ProtClustDB	CLSZ2429605.
Family and domain databases
Gene3D	3.40.30.10. 1 hit.
InterPro	IPR002023. 2Fe-2S_Ferredox. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR10371. PTHR10371. 1 hit.
Pfam	PF01257. Complex1_24kDa. 1 hit. [Graphical view]
PIRSF	PIRSF000216. NADH_DH_24kDa. 1 hit.
SUPFAM	SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR01958. nuoE_fam. 1 hit.
PROSITE	PS01099. COMPLEX1_24K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUV2_DICDI

Accession

Primary (citable) accession number: Q54F10

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)