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Q54EX5

- SPTC2_DICDI

UniProt

Q54EX5 - SPTC2_DICDI

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Protein
Serine palmitoyltransferase 2
Gene
sptB, DDB_G0291283
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic subunit of serine palmitoyltransferase (SPT), which catalyzes the committed step in the synthesis of sphingolipids, the condensation of serine with palmitoyl CoA to form the long chain base 3-ketosphinganine By similarity.

Catalytic activityi

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactori

Pyridoxal phosphate By similarity.

Pathwayi

GO - Molecular functioni

  1. pyridoxal phosphate binding Source: InterPro
  2. serine C-palmitoyltransferase activity Source: dictyBase

GO - Biological processi

  1. sphingolipid biosynthetic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_204726. Sphingolipid de novo biosynthesis.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine palmitoyltransferase 2 (EC:2.3.1.50)
Alternative name(s):
Long chain base biosynthesis protein 2
Short name:
LCB 2
Serine-palmitoyl-CoA transferase 2
Short name:
SPT 2
Short name:
SPT2
Gene namesi
Name:sptB
ORF Names:DDB_G0291283
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291283. sptB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei10 – 3021Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. serine C-palmitoyltransferase complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Serine palmitoyltransferase 2
PRO_0000327871Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei321 – 3211N6-(pyridoxal phosphate)lysine By similarity

Proteomic databases

PRIDEiQ54EX5.

Interactioni

Subunit structurei

Forms a heterodimer with sptA By similarity.

Protein-protein interaction databases

STRINGi44689.DDB_0232041.

Structurei

3D structure databases

ProteinModelPortaliQ54EX5.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0156.
KOiK00654.
OMAiEQEPPYY.
PhylomeDBiQ54EX5.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54EX5-1 [UniParc]FASTAAdd to Basket

« Hide

MEDKEDVENV DDVGYLPILF LYIAYAFIIF NGKLAEFIGK LKGERYLHTP    50
EGYAPLFVEF EYFYQRRMYG RIKDAWDRPI NSIAGAWIDV MPRASKHYSQ 100
RLELTGGKTI KCLNLGSYNY LGFAQNEGPV ADKVIDSIYK YGVYTGSTSA 150
EVGLSEPQRD LENLTARFVG KEDAIVFEMG FATNSGTLPA LIGKGGLIIS 200
DSLNHASLAT GCKNTGCKVK VFRHNDSKHL EEVIRESIIQ GQPRTHRPWT 250
MILIIIEGIY SMEGEVANLP EILAIKKKYK CYLYIDEAHS IGALGKTGRG 300
IVDYYGIDPK EIDILMGTYT KSFGAIGGYV ASDKSLIDHL RQSSFSQVYA 350
NSMSPVCAVQ ALEALRVIMG EDGTDTGAKK LKQLHDNSNY FREKIREMGF 400
VILGNKDSPV IPLMLFNPAK LSAFSRLCLE KHIAVVVVGY PATPLTEPRT 450
RFCISASHTI EDLDWALRQI DEIGDLITLK FHKGKYLKSK 490
Length:490
Mass (Da):54,785
Last modified:May 24, 2005 - v1
Checksum:i052F6E2457F163D8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61625.1.
RefSeqiXP_635115.1. XM_630023.1.

Genome annotation databases

EnsemblProtistsiDDB0232041; DDB0232041; DDB_G0291283.
GeneIDi8628062.
KEGGiddi:DDB_G0291283.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61625.1 .
RefSeqi XP_635115.1. XM_630023.1.

3D structure databases

ProteinModelPortali Q54EX5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0232041.

Proteomic databases

PRIDEi Q54EX5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0232041 ; DDB0232041 ; DDB_G0291283 .
GeneIDi 8628062.
KEGGi ddi:DDB_G0291283.

Organism-specific databases

dictyBasei DDB_G0291283. sptB.

Phylogenomic databases

eggNOGi COG0156.
KOi K00654.
OMAi EQEPPYY.
PhylomeDBi Q54EX5.

Enzyme and pathway databases

UniPathwayi UPA00222 .
Reactomei REACT_204726. Sphingolipid de novo biosynthesis.

Miscellaneous databases

PROi Q54EX5.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Veltman D.M., Insall R.H.
    Submitted (JAN-2010) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 160-167 AND 300-334, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiSPTC2_DICDI
AccessioniPrimary (citable) accession number: Q54EX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi