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Q54EX5 (SPTC2_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine palmitoyltransferase 2

EC=2.3.1.50
Alternative name(s):
Long chain base biosynthesis protein 2
Short name=LCB 2
Serine-palmitoyl-CoA transferase 2
Short name=SPT 2
Short name=SPT2
Gene names
Name:sptB
ORF Names:DDB_G0291283
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalytic subunit of serine palmitoyltransferase (SPT), which catalyzes the committed step in the synthesis of sphingolipids, the condensation of serine with palmitoyl CoA to form the long chain base 3-ketosphinganine By similarity.

Catalytic activity

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Lipid metabolism; sphingolipid metabolism.

Subunit structure

Forms a heterodimer with sptA By similarity.

Subcellular location

Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Serine palmitoyltransferase 2
PRO_0000327871

Regions

Transmembrane10 – 3021Helical; Potential

Amino acid modifications

Modified residue3211N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54EX5 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 052F6E2457F163D8

FASTA49054,785
        10         20         30         40         50         60 
MEDKEDVENV DDVGYLPILF LYIAYAFIIF NGKLAEFIGK LKGERYLHTP EGYAPLFVEF 

        70         80         90        100        110        120 
EYFYQRRMYG RIKDAWDRPI NSIAGAWIDV MPRASKHYSQ RLELTGGKTI KCLNLGSYNY 

       130        140        150        160        170        180 
LGFAQNEGPV ADKVIDSIYK YGVYTGSTSA EVGLSEPQRD LENLTARFVG KEDAIVFEMG 

       190        200        210        220        230        240 
FATNSGTLPA LIGKGGLIIS DSLNHASLAT GCKNTGCKVK VFRHNDSKHL EEVIRESIIQ 

       250        260        270        280        290        300 
GQPRTHRPWT MILIIIEGIY SMEGEVANLP EILAIKKKYK CYLYIDEAHS IGALGKTGRG 

       310        320        330        340        350        360 
IVDYYGIDPK EIDILMGTYT KSFGAIGGYV ASDKSLIDHL RQSSFSQVYA NSMSPVCAVQ 

       370        380        390        400        410        420 
ALEALRVIMG EDGTDTGAKK LKQLHDNSNY FREKIREMGF VILGNKDSPV IPLMLFNPAK 

       430        440        450        460        470        480 
LSAFSRLCLE KHIAVVVVGY PATPLTEPRT RFCISASHTI EDLDWALRQI DEIGDLITLK 

       490 
FHKGKYLKSK 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]Bienvenut W.V., Veltman D.M., Insall R.H.
Submitted (JAN-2010) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 160-167 AND 300-334, IDENTIFICATION BY MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000177 Genomic DNA. Translation: EAL61625.1.
RefSeqXP_635115.1. XM_630023.1.

3D structure databases

ProteinModelPortalQ54EX5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0232041.

Proteomic databases

PRIDEQ54EX5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0232041; DDB0232041; DDB_G0291283.
GeneID8628062.
KEGGddi:DDB_G0291283.

Organism-specific databases

dictyBaseDDB_G0291283. sptB.

Phylogenomic databases

eggNOGCOG0156.
OMAEQEPPYY.
PhylomeDBQ54EX5.

Enzyme and pathway databases

UniPathwayUPA00222.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54EX5.

Entry information

Entry nameSPTC2_DICDI
AccessionPrimary (citable) accession number: Q54EX5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase