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Q54EX5

- SPTC2_DICDI

UniProt

Q54EX5 - SPTC2_DICDI

Protein

Serine palmitoyltransferase 2

Gene

sptB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic subunit of serine palmitoyltransferase (SPT), which catalyzes the committed step in the synthesis of sphingolipids, the condensation of serine with palmitoyl CoA to form the long chain base 3-ketosphinganine.By similarity

    Catalytic activityi

    Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. serine C-palmitoyltransferase activity Source: dictyBase

    GO - Biological processi

    1. sphingolipid biosynthetic process Source: dictyBase

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_204726. Sphingolipid de novo biosynthesis.
    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine palmitoyltransferase 2 (EC:2.3.1.50)
    Alternative name(s):
    Long chain base biosynthesis protein 2
    Short name:
    LCB 2
    Serine-palmitoyl-CoA transferase 2
    Short name:
    SPT 2
    Short name:
    SPT2
    Gene namesi
    Name:sptB
    ORF Names:DDB_G0291283
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0291283. sptB.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. serine C-palmitoyltransferase complex Source: dictyBase

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 490490Serine palmitoyltransferase 2PRO_0000327871Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei321 – 3211N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    PRIDEiQ54EX5.

    Interactioni

    Subunit structurei

    Forms a heterodimer with sptA.By similarity

    Protein-protein interaction databases

    STRINGi44689.DDB_0232041.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54EX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0156.
    KOiK00654.
    OMAiEQEPPYY.
    PhylomeDBiQ54EX5.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54EX5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDKEDVENV DDVGYLPILF LYIAYAFIIF NGKLAEFIGK LKGERYLHTP    50
    EGYAPLFVEF EYFYQRRMYG RIKDAWDRPI NSIAGAWIDV MPRASKHYSQ 100
    RLELTGGKTI KCLNLGSYNY LGFAQNEGPV ADKVIDSIYK YGVYTGSTSA 150
    EVGLSEPQRD LENLTARFVG KEDAIVFEMG FATNSGTLPA LIGKGGLIIS 200
    DSLNHASLAT GCKNTGCKVK VFRHNDSKHL EEVIRESIIQ GQPRTHRPWT 250
    MILIIIEGIY SMEGEVANLP EILAIKKKYK CYLYIDEAHS IGALGKTGRG 300
    IVDYYGIDPK EIDILMGTYT KSFGAIGGYV ASDKSLIDHL RQSSFSQVYA 350
    NSMSPVCAVQ ALEALRVIMG EDGTDTGAKK LKQLHDNSNY FREKIREMGF 400
    VILGNKDSPV IPLMLFNPAK LSAFSRLCLE KHIAVVVVGY PATPLTEPRT 450
    RFCISASHTI EDLDWALRQI DEIGDLITLK FHKGKYLKSK 490
    Length:490
    Mass (Da):54,785
    Last modified:May 24, 2005 - v1
    Checksum:i052F6E2457F163D8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000177 Genomic DNA. Translation: EAL61625.1.
    RefSeqiXP_635115.1. XM_630023.1.

    Genome annotation databases

    EnsemblProtistsiDDB0232041; DDB0232041; DDB_G0291283.
    GeneIDi8628062.
    KEGGiddi:DDB_G0291283.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000177 Genomic DNA. Translation: EAL61625.1 .
    RefSeqi XP_635115.1. XM_630023.1.

    3D structure databases

    ProteinModelPortali Q54EX5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0232041.

    Proteomic databases

    PRIDEi Q54EX5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0232041 ; DDB0232041 ; DDB_G0291283 .
    GeneIDi 8628062.
    KEGGi ddi:DDB_G0291283.

    Organism-specific databases

    dictyBasei DDB_G0291283. sptB.

    Phylogenomic databases

    eggNOGi COG0156.
    KOi K00654.
    OMAi EQEPPYY.
    PhylomeDBi Q54EX5.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    Reactomei REACT_204726. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    PROi Q54EX5.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. Bienvenut W.V., Veltman D.M., Insall R.H.
      Submitted (JAN-2010) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 160-167 AND 300-334, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiSPTC2_DICDI
    AccessioniPrimary (citable) accession number: Q54EX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3