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Q54EW8

- DLDH_DICDI

UniProt

Q54EW8 - DLDH_DICDI

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Protein

Dihydrolipoyl dehydrogenase, mitochondrial

Gene

lpd

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Protein N(6)-(dihydrolipoyl)lysine + NAD+ = protein N(6)-(lipoyl)lysine + NADH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701FADBy similarity
Binding sitei134 – 1341FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei223 – 2231NADBy similarity
Binding sitei257 – 2571NAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei294 – 2941NAD; via amide nitrogenBy similarity
Binding sitei335 – 3351FADBy similarity
Active sitei467 – 4671Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi52 – 6110FADBy similarity
Nucleotide bindingi163 – 1653FADBy similarity
Nucleotide bindingi200 – 2078NADBy similarity
Nucleotide bindingi341 – 3444FADBy similarity

GO - Molecular functioni

  1. dihydrolipoyl dehydrogenase activity Source: dictyBase
  2. flavin adenine dinucleotide binding Source: InterPro

GO - Biological processi

  1. acetyl-CoA biosynthetic process from pyruvate Source: dictyBase
  2. cell redox homeostasis Source: InterPro
  3. glycine catabolic process Source: dictyBase
  4. isoleucine catabolic process Source: dictyBase
  5. leucine catabolic process Source: dictyBase
  6. L-serine biosynthetic process Source: dictyBase
  7. valine catabolic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrolipoyl dehydrogenase, mitochondrial (EC:1.8.1.4)
Alternative name(s):
Dihydrolipoamide dehydrogenase
Glycine cleavage system L protein
Gene namesi
Name:lpd
Synonyms:bkdD, dld, odhC, pdhD
ORF Names:DDB_G0291648
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291648. lpd.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial matrix Source: dictyBase
  2. mitochondrial pyruvate dehydrogenase complex Source: dictyBase
  3. phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525MitochondrionSequence AnalysisAdd
BLAST
Chaini26 – 488463Dihydrolipoyl dehydrogenase, mitochondrialPRO_0000327450Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi61 ↔ 66Redox-activeBy similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ54EW8.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0216232.

Structurei

3D structure databases

ProteinModelPortaliQ54EW8.
SMRiQ54EW8. Positions 20-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiCOG1249.
InParanoidiQ54EW8.
KOiK00382.
OMAiSNDGALR.
PhylomeDBiQ54EW8.

Family and domain databases

Gene3Di3.30.390.30. 1 hit.
InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006258. Lipoamide_DH.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view]
PfamiPF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view]
PRINTSiPR00368. FADPNR.
SUPFAMiSSF55424. SSF55424. 1 hit.
TIGRFAMsiTIGR01350. lipoamide_DH. 1 hit.
PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54EW8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRINRISNL RTFGQRFFST EQQDVVVIGG GPGGYVAGIK AGQLGMKVTV
60 70 80 90 100
VEKRGKLGGT CLNVGCIPSK ALLNASHLYE EATTKMSKYG VKCSGVELDL
110 120 130 140 150
GAMMQYKDKS VSGLTSGIEG LFKKNKVKYD KGFGKITGPN TVEVTLNDGS
160 170 180 190 200
VKTIETKNIV IATGSEVTSL PNVNIDEESI ISSTGALALK SVPKKLIVIG
210 220 230 240 250
GGVIGLELGS VWSRLGSETT VVEFTNRIAA GADGEVAKKF QKSLEKQHMK
260 270 280 290 300
FHLETKVTSV VKKSDGKVTV TVEQVGAGGF TGTLEADAVL VSVGRRPNTS
310 320 330 340 350
GLGLESVGIP TDKAGRVEVG DHFNTKVPSI FAIGDAIRGP MLAHKAEEEG
360 370 380 390 400
IAIIEQIHNG GGHVNYGAIP SIIYTHPEVA WVGKTEEELQ KEGIQYNIGR
410 420 430 440 450
FPFVANSRAK TNDDVEGFVK FLAAKDSDRV LGAHIMGTNA GELIGECVLA
460 470 480
MEYGASCEDI ARTCHGHPTL SEAVKEAAMD AYDKPIHM
Length:488
Mass (Da):51,794
Last modified:May 24, 2005 - v1
Checksum:iA7DD67E8F7707783
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61808.1.
AY466389 Genomic DNA. Translation: AAS47709.1.
RefSeqiXP_635122.1. XM_630030.1.

Genome annotation databases

EnsemblProtistsiDDB0216232; DDB0216232; DDB_G0291648.
GeneIDi8628069.
KEGGiddi:DDB_G0291648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61808.1 .
AY466389 Genomic DNA. Translation: AAS47709.1 .
RefSeqi XP_635122.1. XM_630030.1.

3D structure databases

ProteinModelPortali Q54EW8.
SMRi Q54EW8. Positions 20-488.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0216232.

Proteomic databases

PRIDEi Q54EW8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0216232 ; DDB0216232 ; DDB_G0291648 .
GeneIDi 8628069.
KEGGi ddi:DDB_G0291648.

Organism-specific databases

dictyBasei DDB_G0291648. lpd.

Phylogenomic databases

eggNOGi COG1249.
InParanoidi Q54EW8.
KOi K00382.
OMAi SNDGALR.
PhylomeDBi Q54EW8.

Miscellaneous databases

PROi Q54EW8.

Family and domain databases

Gene3Di 3.30.390.30. 1 hit.
InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
IPR013027. FAD_pyr_nucl-diS_OxRdtase.
IPR006258. Lipoamide_DH.
IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
IPR012999. Pyr_OxRdtase_I_AS.
IPR001327. Pyr_OxRdtase_NAD-bd_dom.
[Graphical view ]
Pfami PF00070. Pyr_redox. 1 hit.
PF07992. Pyr_redox_2. 1 hit.
PF02852. Pyr_redox_dim. 1 hit.
[Graphical view ]
PRINTSi PR00368. FADPNR.
SUPFAMi SSF55424. SSF55424. 1 hit.
TIGRFAMsi TIGR01350. lipoamide_DH. 1 hit.
PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "A first glimpse into the pattern and scale of gene transfer in Apicomplexa."
    Huang J., Mullapudi N., Sicheritz-Ponten T., Kissinger J.C.
    Int. J. Parasitol. 34:265-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-357.

Entry informationi

Entry nameiDLDH_DICDI
AccessioniPrimary (citable) accession number: Q54EW8
Secondary accession number(s): Q6S4V6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The active site is a redox-active disulfide bond.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3