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Q54EW8

- DLDH_DICDI

UniProt

Q54EW8 - DLDH_DICDI

Protein

Dihydrolipoyl dehydrogenase, mitochondrial

Gene

lpd

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Protein N(6)-(dihydrolipoyl)lysine + NAD+ = protein N(6)-(lipoyl)lysine + NADH.

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei70 – 701FADBy similarity
    Binding sitei134 – 1341FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei223 – 2231NADBy similarity
    Binding sitei257 – 2571NAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei294 – 2941NAD; via amide nitrogenBy similarity
    Binding sitei335 – 3351FADBy similarity
    Active sitei467 – 4671Proton acceptorBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi52 – 6110FADBy similarity
    Nucleotide bindingi163 – 1653FADBy similarity
    Nucleotide bindingi200 – 2078NADBy similarity
    Nucleotide bindingi341 – 3444FADBy similarity

    GO - Molecular functioni

    1. dihydrolipoyl dehydrogenase activity Source: dictyBase
    2. flavin adenine dinucleotide binding Source: InterPro

    GO - Biological processi

    1. acetyl-CoA biosynthetic process from pyruvate Source: dictyBase
    2. cell redox homeostasis Source: InterPro
    3. glycine catabolic process Source: dictyBase
    4. isoleucine catabolic process Source: dictyBase
    5. leucine catabolic process Source: dictyBase
    6. L-serine biosynthetic process Source: dictyBase
    7. valine catabolic process Source: dictyBase

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrolipoyl dehydrogenase, mitochondrial (EC:1.8.1.4)
    Alternative name(s):
    Dihydrolipoamide dehydrogenase
    Glycine cleavage system L protein
    Gene namesi
    Name:lpd
    Synonyms:bkdD, dld, odhC, pdhD
    ORF Names:DDB_G0291648
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0291648. lpd.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: dictyBase
    2. mitochondrial pyruvate dehydrogenase complex Source: dictyBase
    3. phagocytic vesicle Source: dictyBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2525MitochondrionSequence AnalysisAdd
    BLAST
    Chaini26 – 488463Dihydrolipoyl dehydrogenase, mitochondrialPRO_0000327450Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi61 ↔ 66Redox-activeBy similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiQ54EW8.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0216232.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54EW8.
    SMRiQ54EW8. Positions 20-488.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Redox-active center, Transit peptide

    Phylogenomic databases

    eggNOGiCOG1249.
    KOiK00382.
    OMAiSNDGALR.
    PhylomeDBiQ54EW8.

    Family and domain databases

    Gene3Di3.30.390.30. 1 hit.
    InterProiIPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR006258. Lipoamide_DH.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR012999. Pyr_OxRdtase_I_AS.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view]
    PfamiPF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view]
    PRINTSiPR00368. FADPNR.
    SUPFAMiSSF55424. SSF55424. 1 hit.
    TIGRFAMsiTIGR01350. lipoamide_DH. 1 hit.
    PROSITEiPS00076. PYRIDINE_REDOX_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54EW8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRINRISNL RTFGQRFFST EQQDVVVIGG GPGGYVAGIK AGQLGMKVTV    50
    VEKRGKLGGT CLNVGCIPSK ALLNASHLYE EATTKMSKYG VKCSGVELDL 100
    GAMMQYKDKS VSGLTSGIEG LFKKNKVKYD KGFGKITGPN TVEVTLNDGS 150
    VKTIETKNIV IATGSEVTSL PNVNIDEESI ISSTGALALK SVPKKLIVIG 200
    GGVIGLELGS VWSRLGSETT VVEFTNRIAA GADGEVAKKF QKSLEKQHMK 250
    FHLETKVTSV VKKSDGKVTV TVEQVGAGGF TGTLEADAVL VSVGRRPNTS 300
    GLGLESVGIP TDKAGRVEVG DHFNTKVPSI FAIGDAIRGP MLAHKAEEEG 350
    IAIIEQIHNG GGHVNYGAIP SIIYTHPEVA WVGKTEEELQ KEGIQYNIGR 400
    FPFVANSRAK TNDDVEGFVK FLAAKDSDRV LGAHIMGTNA GELIGECVLA 450
    MEYGASCEDI ARTCHGHPTL SEAVKEAAMD AYDKPIHM 488
    Length:488
    Mass (Da):51,794
    Last modified:May 24, 2005 - v1
    Checksum:iA7DD67E8F7707783
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000177 Genomic DNA. Translation: EAL61808.1.
    AY466389 Genomic DNA. Translation: AAS47709.1.
    RefSeqiXP_635122.1. XM_630030.1.

    Genome annotation databases

    EnsemblProtistsiDDB0216232; DDB0216232; DDB_G0291648.
    GeneIDi8628069.
    KEGGiddi:DDB_G0291648.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000177 Genomic DNA. Translation: EAL61808.1 .
    AY466389 Genomic DNA. Translation: AAS47709.1 .
    RefSeqi XP_635122.1. XM_630030.1.

    3D structure databases

    ProteinModelPortali Q54EW8.
    SMRi Q54EW8. Positions 20-488.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0216232.

    Proteomic databases

    PRIDEi Q54EW8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0216232 ; DDB0216232 ; DDB_G0291648 .
    GeneIDi 8628069.
    KEGGi ddi:DDB_G0291648.

    Organism-specific databases

    dictyBasei DDB_G0291648. lpd.

    Phylogenomic databases

    eggNOGi COG1249.
    KOi K00382.
    OMAi SNDGALR.
    PhylomeDBi Q54EW8.

    Miscellaneous databases

    PROi Q54EW8.

    Family and domain databases

    Gene3Di 3.30.390.30. 1 hit.
    InterProi IPR016156. FAD/NAD-linked_Rdtase_dimer.
    IPR013027. FAD_pyr_nucl-diS_OxRdtase.
    IPR006258. Lipoamide_DH.
    IPR004099. Pyr_nucl-diS_OxRdtase_dimer.
    IPR023753. Pyr_nucl-diS_OxRdtase_FAD/NAD.
    IPR012999. Pyr_OxRdtase_I_AS.
    IPR001327. Pyr_OxRdtase_NAD-bd_dom.
    [Graphical view ]
    Pfami PF00070. Pyr_redox. 1 hit.
    PF07992. Pyr_redox_2. 1 hit.
    PF02852. Pyr_redox_dim. 1 hit.
    [Graphical view ]
    PRINTSi PR00368. FADPNR.
    SUPFAMi SSF55424. SSF55424. 1 hit.
    TIGRFAMsi TIGR01350. lipoamide_DH. 1 hit.
    PROSITEi PS00076. PYRIDINE_REDOX_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "A first glimpse into the pattern and scale of gene transfer in Apicomplexa."
      Huang J., Mullapudi N., Sicheritz-Ponten T., Kissinger J.C.
      Int. J. Parasitol. 34:265-274(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-357.

    Entry informationi

    Entry nameiDLDH_DICDI
    AccessioniPrimary (citable) accession number: Q54EW8
    Secondary accession number(s): Q6S4V6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    The active site is a redox-active disulfide bond.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3