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Q54EW2

- Y9130_DICDI

UniProt

Q54EW2 - Y9130_DICDI

Protein

Putative bifunctional amine oxidase DDB_G0291301

Gene

DDB_G0291301

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids. Metabolizes sarcosine, L-pipecolic acid and L-proline By similarity.By similarity

    Catalytic activityi

    Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.
    L-pipecolate + O2 = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2.
    An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

    Cofactori

    FAD.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei535 – 5351FADBy similarity
    Binding sitei544 – 5441FADBy similarity
    Binding sitei886 – 8861SubstrateBy similarity
    Binding sitei978 – 9781FADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 4031FADSequence AnalysisAdd
    BLAST
    Nucleotide bindingi563 – 5642FADBy similarity
    Nucleotide bindingi987 – 9904FADBy similarity

    GO - Molecular functioni

    1. L-amino-acid oxidase activity Source: UniProtKB-EC
    2. L-pipecolate oxidase activity Source: UniProtKB-EC
    3. sarcosine oxidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    ReactomeiREACT_176494. Abnormal metabolism in phenylketonuria.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative bifunctional amine oxidase DDB_G0291301
    Including the following 2 domains:
    Putative sarcosine oxidase (EC:1.5.3.1, EC:1.5.3.7)
    Short name:
    PSO
    Putative L-amino-acid oxidase (EC:1.4.3.2)
    Gene namesi
    ORF Names:DDB_G0291301
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0291301.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10801080Putative bifunctional amine oxidase DDB_G0291301PRO_0000388375Add
    BLAST

    Proteomic databases

    PRIDEiQ54EW2.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDBDRAFT_0183806.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54EW2.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei508 – 52821HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – ?450450Putative sarcosine oxidaseAdd
    BLAST
    Regioni?450 – 1080631Putative L-amino-acid oxidaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the MSOX/MTOX family.Curated
    In the C-terminal section; belongs to the flavin monoamine oxidase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0665.
    InParanoidiQ54EW2.
    OMAiRTMYTED.
    PhylomeDBiQ54EW2.

    Family and domain databases

    InterProiIPR002937. Amino_oxidase.
    IPR006076. FAD-dep_OxRdtase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    PF01266. DAO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q54EW2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MREFLKDDYD VIVCGGGPVG LATAYRCAKA GKKVLCLEKS VFFNGGGSSG     50
    DVVRMLRTMY TEDYMADLAH ETLGLWKELG DDAGEGDLVW MTGLLNFGDP 100
    NYGAGGPEGT LLGPIPNLER LGMQYKVLTA QEIMEEYPFR NIPSNHQGVF 150
    APDNGVINLP LVLRSLYKLC LQYGCKMVSH AEVKLIKNLS TTMVEIEVEH 200
    TDVDQKNKQS FKVKSKKAAI TSNSFCNHII KPSFGWELDM TIWEMTSSYF 250
    VAKPGPNATV FKSMWFNFQN DTDNDPTKSN LYYGFPAVPW MTDNHCRIAL 300
    DAALRQIKDP NDRHDGVETH DVNRTRDWVR EHIPGVDDTP LFNVSALMAN 350
    VYDNMFVLDF IPETNNNVVM FACGWAMKFI PLLGKILSQL LDEGKTQYPI 400
    DHFALNRGNG ALIIKEGQTP KSVNSQVRAP RYTSMHCKPL TIAKSTVPTN 450
    QSSNPDGASS TAPTQSLRSL FASRLLQRSV GMEAKFHSII AKNRSKRSTK 500
    ASQKDLTVGI IGAGMAGLYA AMILQDLGLQ YNILEANKER VGGRIYTYRF 550
    PQNQDKYQTV ELGAMRFPKI EIMDRLLNLD KPWSLFSKLE KAGHKIPTIP 600
    YHLTVDNNLV YYNGKRIFAN TLLNDDPLYF SDTHNGGPGT AVPDKYTYQP 650
    YGDLLDAVYK KFSDDLENDF ESGFETLLKS DNYSTRAYLF EKGPYPQSVV 700
    NYLETMDTGT GLYDMAFSET IMDYFDFSAG DEWLCIDGGT DIIVNSMVKT 750
    LRPGCIEQGK VVTKVSRVVG KSGDVSNLKV DFLDGTEGRL FKHVISTGTL 800
    ASLRRVDLSD LKLSHNKRTA IRSLHYDHSV KIALSFKSRW WEDSKFMNGK 850
    PMLGGKSSTD LPVRTIVYPS YGIGQPGVSG VLIVSYTWSL DASRIGSLVG 900
    DRPSEEVLIK LCMANLAEVH NVPVATLQQL FVDYKCWDWY NDDYSSGAFA 950
    LYSPSQFSQL FPSLTKPSPD GRFHLAGEAT SVHHGWVIGS LNSAYRSVDH 1000
    ILQVEGLDEL RAKLRLNWGF IDEVEDPQDD QQYVDPHHNL SGPNAKKFRS 1050
    NVSQVAIQPR FKAPRNFKPR NTAASIGGLK 1080
    Length:1,080
    Mass (Da):120,674
    Last modified:May 24, 2005 - v1
    Checksum:iFB0C9DA09FC71D6D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000177 Genomic DNA. Translation: EAL61634.1.
    RefSeqiXP_635128.1. XM_630036.1.

    Genome annotation databases

    EnsemblProtistsiDDB0235252; DDB0235252; DDB_G0291301.
    GeneIDi8628074.
    KEGGiddi:DDB_G0291301.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000177 Genomic DNA. Translation: EAL61634.1 .
    RefSeqi XP_635128.1. XM_630036.1.

    3D structure databases

    ProteinModelPortali Q54EW2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDBDRAFT_0183806.

    Proteomic databases

    PRIDEi Q54EW2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0235252 ; DDB0235252 ; DDB_G0291301 .
    GeneIDi 8628074.
    KEGGi ddi:DDB_G0291301.

    Organism-specific databases

    dictyBasei DDB_G0291301.

    Phylogenomic databases

    eggNOGi COG0665.
    InParanoidi Q54EW2.
    OMAi RTMYTED.
    PhylomeDBi Q54EW2.

    Enzyme and pathway databases

    Reactomei REACT_176494. Abnormal metabolism in phenylketonuria.

    Family and domain databases

    InterProi IPR002937. Amino_oxidase.
    IPR006076. FAD-dep_OxRdtase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    PF01266. DAO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. Bienvenut W.V., Ura S., Insall R.H.
      Submitted (JUL-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 40-54; 445-468 AND 1050-1060, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: AX2.

    Entry informationi

    Entry nameiY9130_DICDI
    AccessioniPrimary (citable) accession number: Q54EW2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3