Q54EW2 (Y9130_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative bifunctional amine oxidase DDB_G0291301

Including the following 2 domains:

1. Putative sarcosine oxidase
   Short name=PSO
   EC=1.5.3.1
   EC=1.5.3.7
2. Putative L-amino-acid oxidase
   EC=1.4.3.2

Gene names

ORF Names:

DDB_G0291301

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1080 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids. Metabolizes sarcosine, L-pipecolic acid and L-proline By similarity.
Catalytic activity	Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2. L-pipecolate + O2 = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2. An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.
Cofactor	FAD By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the MSOX/MTOX family. In the C-terminal section; belongs to the flavin monoamine oxidase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular space Inferred from direct assay PubMed 20422638. Source: dictyBase integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-amino-acid oxidase activity Inferred from electronic annotation. Source: EC L-pipecolate oxidase activity Inferred from electronic annotation. Source: EC sarcosine oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1080	1080	Putative bifunctional amine oxidase DDB_G0291301		PRO_0000388375
Regions
	Transmembrane	508 – 528	21	Helical; Potential
	Nucleotide binding	10 – 40	31	FAD Potential
	Nucleotide binding	563 – 564	2	FAD By similarity
	Nucleotide binding	987 – 990	4	FAD By similarity
	Region	1 – ?450	450	Putative sarcosine oxidase
	Region	?450 – 1080	631	Putative L-amino-acid oxidase
Sites
	Binding site	535	1	FAD By similarity
	Binding site	544	1	FAD By similarity
	Binding site	886	1	Substrate By similarity
	Binding site	978	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54EW2 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: FB0C9DA09FC71D6D	FASTA	1,080	120,674
	10 20 30 40 50 60 MREFLKDDYD VIVCGGGPVG LATAYRCAKA GKKVLCLEKS VFFNGGGSSG DVVRMLRTMY 70 80 90 100 110 120 TEDYMADLAH ETLGLWKELG DDAGEGDLVW MTGLLNFGDP NYGAGGPEGT LLGPIPNLER 130 140 150 160 170 180 LGMQYKVLTA QEIMEEYPFR NIPSNHQGVF APDNGVINLP LVLRSLYKLC LQYGCKMVSH 190 200 210 220 230 240 AEVKLIKNLS TTMVEIEVEH TDVDQKNKQS FKVKSKKAAI TSNSFCNHII KPSFGWELDM 250 260 270 280 290 300 TIWEMTSSYF VAKPGPNATV FKSMWFNFQN DTDNDPTKSN LYYGFPAVPW MTDNHCRIAL 310 320 330 340 350 360 DAALRQIKDP NDRHDGVETH DVNRTRDWVR EHIPGVDDTP LFNVSALMAN VYDNMFVLDF 370 380 390 400 410 420 IPETNNNVVM FACGWAMKFI PLLGKILSQL LDEGKTQYPI DHFALNRGNG ALIIKEGQTP 430 440 450 460 470 480 KSVNSQVRAP RYTSMHCKPL TIAKSTVPTN QSSNPDGASS TAPTQSLRSL FASRLLQRSV 490 500 510 520 530 540 GMEAKFHSII AKNRSKRSTK ASQKDLTVGI IGAGMAGLYA AMILQDLGLQ YNILEANKER 550 560 570 580 590 600 VGGRIYTYRF PQNQDKYQTV ELGAMRFPKI EIMDRLLNLD KPWSLFSKLE KAGHKIPTIP 610 620 630 640 650 660 YHLTVDNNLV YYNGKRIFAN TLLNDDPLYF SDTHNGGPGT AVPDKYTYQP YGDLLDAVYK 670 680 690 700 710 720 KFSDDLENDF ESGFETLLKS DNYSTRAYLF EKGPYPQSVV NYLETMDTGT GLYDMAFSET 730 740 750 760 770 780 IMDYFDFSAG DEWLCIDGGT DIIVNSMVKT LRPGCIEQGK VVTKVSRVVG KSGDVSNLKV 790 800 810 820 830 840 DFLDGTEGRL FKHVISTGTL ASLRRVDLSD LKLSHNKRTA IRSLHYDHSV KIALSFKSRW 850 860 870 880 890 900 WEDSKFMNGK PMLGGKSSTD LPVRTIVYPS YGIGQPGVSG VLIVSYTWSL DASRIGSLVG 910 920 930 940 950 960 DRPSEEVLIK LCMANLAEVH NVPVATLQQL FVDYKCWDWY NDDYSSGAFA LYSPSQFSQL 970 980 990 1000 1010 1020 FPSLTKPSPD GRFHLAGEAT SVHHGWVIGS LNSAYRSVDH ILQVEGLDEL RAKLRLNWGF 1030 1040 1050 1060 1070 1080 IDEVEDPQDD QQYVDPHHNL SGPNAKKFRS NVSQVAIQPR FKAPRNFKPR NTAASIGGLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	Bienvenut W.V., Ura S., Insall R.H. Submitted (JUL-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 40-54; 445-468 AND 1050-1060, MASS SPECTROMETRY. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000177 Genomic DNA. Translation: EAL61634.1.
RefSeq	XP_635128.1. XM_630036.1.
3D structure databases
ProteinModelPortal	Q54EW2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0235252; DDB0235252; DDB_G0291301.
GeneID	8628074.
KEGG	ddi:DDB_G0291301.
Organism-specific databases
dictyBase	DDB_G0291301.
Phylogenomic databases
eggNOG	COG0665.
InParanoid	Q54EW2.
OMA	PDNGVIN.
ProtClustDB	CLSZ2429256.
Family and domain databases
InterPro	IPR002937. Amino_oxidase. IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y9130_DICDI

Accession

Primary (citable) accession number: Q54EW2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 24, 2005
Last modified:	March 6, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)