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Q54EW2

- Y9130_DICDI

UniProt

Q54EW2 - Y9130_DICDI

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Protein

Putative bifunctional amine oxidase DDB_G0291301

Gene

DDB_G0291301

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids. Metabolizes sarcosine, L-pipecolic acid and L-proline (By similarity).By similarity

Catalytic activityi

Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.
L-pipecolate + O2 = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2.
An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei535 – 5351FADBy similarity
Binding sitei544 – 5441FADBy similarity
Binding sitei886 – 8861SubstrateBy similarity
Binding sitei978 – 9781FADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 4031FADSequence AnalysisAdd
BLAST
Nucleotide bindingi563 – 5642FADBy similarity
Nucleotide bindingi987 – 9904FADBy similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC
  2. L-pipecolate oxidase activity Source: UniProtKB-EC
  3. sarcosine oxidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_176494. Abnormal metabolism in phenylketonuria.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative bifunctional amine oxidase DDB_G0291301
Including the following 2 domains:
Putative sarcosine oxidase (EC:1.5.3.1, EC:1.5.3.7)
Short name:
PSO
Putative L-amino-acid oxidase (EC:1.4.3.2)
Gene namesi
ORF Names:DDB_G0291301
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291301.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10801080Putative bifunctional amine oxidase DDB_G0291301PRO_0000388375Add
BLAST

Proteomic databases

PRIDEiQ54EW2.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0183806.

Structurei

3D structure databases

ProteinModelPortaliQ54EW2.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei508 – 52821HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – ?450450Putative sarcosine oxidaseAdd
BLAST
Regioni?450 – 1080631Putative L-amino-acid oxidaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the MSOX/MTOX family.Curated
In the C-terminal section; belongs to the flavin monoamine oxidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0665.
InParanoidiQ54EW2.
OMAiRTMYTED.
PhylomeDBiQ54EW2.

Family and domain databases

InterProiIPR002937. Amino_oxidase.
IPR006076. FAD-dep_OxRdtase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
PF01266. DAO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54EW2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MREFLKDDYD VIVCGGGPVG LATAYRCAKA GKKVLCLEKS VFFNGGGSSG
60 70 80 90 100
DVVRMLRTMY TEDYMADLAH ETLGLWKELG DDAGEGDLVW MTGLLNFGDP
110 120 130 140 150
NYGAGGPEGT LLGPIPNLER LGMQYKVLTA QEIMEEYPFR NIPSNHQGVF
160 170 180 190 200
APDNGVINLP LVLRSLYKLC LQYGCKMVSH AEVKLIKNLS TTMVEIEVEH
210 220 230 240 250
TDVDQKNKQS FKVKSKKAAI TSNSFCNHII KPSFGWELDM TIWEMTSSYF
260 270 280 290 300
VAKPGPNATV FKSMWFNFQN DTDNDPTKSN LYYGFPAVPW MTDNHCRIAL
310 320 330 340 350
DAALRQIKDP NDRHDGVETH DVNRTRDWVR EHIPGVDDTP LFNVSALMAN
360 370 380 390 400
VYDNMFVLDF IPETNNNVVM FACGWAMKFI PLLGKILSQL LDEGKTQYPI
410 420 430 440 450
DHFALNRGNG ALIIKEGQTP KSVNSQVRAP RYTSMHCKPL TIAKSTVPTN
460 470 480 490 500
QSSNPDGASS TAPTQSLRSL FASRLLQRSV GMEAKFHSII AKNRSKRSTK
510 520 530 540 550
ASQKDLTVGI IGAGMAGLYA AMILQDLGLQ YNILEANKER VGGRIYTYRF
560 570 580 590 600
PQNQDKYQTV ELGAMRFPKI EIMDRLLNLD KPWSLFSKLE KAGHKIPTIP
610 620 630 640 650
YHLTVDNNLV YYNGKRIFAN TLLNDDPLYF SDTHNGGPGT AVPDKYTYQP
660 670 680 690 700
YGDLLDAVYK KFSDDLENDF ESGFETLLKS DNYSTRAYLF EKGPYPQSVV
710 720 730 740 750
NYLETMDTGT GLYDMAFSET IMDYFDFSAG DEWLCIDGGT DIIVNSMVKT
760 770 780 790 800
LRPGCIEQGK VVTKVSRVVG KSGDVSNLKV DFLDGTEGRL FKHVISTGTL
810 820 830 840 850
ASLRRVDLSD LKLSHNKRTA IRSLHYDHSV KIALSFKSRW WEDSKFMNGK
860 870 880 890 900
PMLGGKSSTD LPVRTIVYPS YGIGQPGVSG VLIVSYTWSL DASRIGSLVG
910 920 930 940 950
DRPSEEVLIK LCMANLAEVH NVPVATLQQL FVDYKCWDWY NDDYSSGAFA
960 970 980 990 1000
LYSPSQFSQL FPSLTKPSPD GRFHLAGEAT SVHHGWVIGS LNSAYRSVDH
1010 1020 1030 1040 1050
ILQVEGLDEL RAKLRLNWGF IDEVEDPQDD QQYVDPHHNL SGPNAKKFRS
1060 1070 1080
NVSQVAIQPR FKAPRNFKPR NTAASIGGLK
Length:1,080
Mass (Da):120,674
Last modified:May 24, 2005 - v1
Checksum:iFB0C9DA09FC71D6D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61634.1.
RefSeqiXP_635128.1. XM_630036.1.

Genome annotation databases

EnsemblProtistsiDDB0235252; DDB0235252; DDB_G0291301.
GeneIDi8628074.
KEGGiddi:DDB_G0291301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61634.1 .
RefSeqi XP_635128.1. XM_630036.1.

3D structure databases

ProteinModelPortali Q54EW2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0183806.

Proteomic databases

PRIDEi Q54EW2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0235252 ; DDB0235252 ; DDB_G0291301 .
GeneIDi 8628074.
KEGGi ddi:DDB_G0291301.

Organism-specific databases

dictyBasei DDB_G0291301.

Phylogenomic databases

eggNOGi COG0665.
InParanoidi Q54EW2.
OMAi RTMYTED.
PhylomeDBi Q54EW2.

Enzyme and pathway databases

Reactomei REACT_176494. Abnormal metabolism in phenylketonuria.

Family and domain databases

InterProi IPR002937. Amino_oxidase.
IPR006076. FAD-dep_OxRdtase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
PF01266. DAO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Ura S., Insall R.H.
    Submitted (JUL-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 40-54; 445-468 AND 1050-1060, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: AX2.

Entry informationi

Entry nameiY9130_DICDI
AccessioniPrimary (citable) accession number: Q54EW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 24, 2005
Last modified: October 1, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3