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Q54EW2 (Y9130_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative bifunctional amine oxidase DDB_G0291301

Including the following 2 domains:

  1. Putative sarcosine oxidase
    Short name=PSO
    EC=1.5.3.1
    EC=1.5.3.7
  2. Putative L-amino-acid oxidase
    EC=1.4.3.2
Gene names
ORF Names:DDB_G0291301
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1080 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids. Metabolizes sarcosine, L-pipecolic acid and L-proline By similarity.

Catalytic activity

Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.

L-pipecolate + O2 = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2.

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactor

FAD By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

In the N-terminal section; belongs to the MSOX/MTOX family.

In the C-terminal section; belongs to the flavin monoamine oxidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10801080Putative bifunctional amine oxidase DDB_G0291301
PRO_0000388375

Regions

Transmembrane508 – 52821Helical; Potential
Nucleotide binding10 – 4031FAD Potential
Nucleotide binding563 – 5642FAD By similarity
Nucleotide binding987 – 9904FAD By similarity
Region1 – ?450450Putative sarcosine oxidase
Region?450 – 1080631Putative L-amino-acid oxidase

Sites

Binding site5351FAD By similarity
Binding site5441FAD By similarity
Binding site8861Substrate By similarity
Binding site9781FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54EW2 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: FB0C9DA09FC71D6D

FASTA1,080120,674
        10         20         30         40         50         60 
MREFLKDDYD VIVCGGGPVG LATAYRCAKA GKKVLCLEKS VFFNGGGSSG DVVRMLRTMY 

        70         80         90        100        110        120 
TEDYMADLAH ETLGLWKELG DDAGEGDLVW MTGLLNFGDP NYGAGGPEGT LLGPIPNLER 

       130        140        150        160        170        180 
LGMQYKVLTA QEIMEEYPFR NIPSNHQGVF APDNGVINLP LVLRSLYKLC LQYGCKMVSH 

       190        200        210        220        230        240 
AEVKLIKNLS TTMVEIEVEH TDVDQKNKQS FKVKSKKAAI TSNSFCNHII KPSFGWELDM 

       250        260        270        280        290        300 
TIWEMTSSYF VAKPGPNATV FKSMWFNFQN DTDNDPTKSN LYYGFPAVPW MTDNHCRIAL 

       310        320        330        340        350        360 
DAALRQIKDP NDRHDGVETH DVNRTRDWVR EHIPGVDDTP LFNVSALMAN VYDNMFVLDF 

       370        380        390        400        410        420 
IPETNNNVVM FACGWAMKFI PLLGKILSQL LDEGKTQYPI DHFALNRGNG ALIIKEGQTP 

       430        440        450        460        470        480 
KSVNSQVRAP RYTSMHCKPL TIAKSTVPTN QSSNPDGASS TAPTQSLRSL FASRLLQRSV 

       490        500        510        520        530        540 
GMEAKFHSII AKNRSKRSTK ASQKDLTVGI IGAGMAGLYA AMILQDLGLQ YNILEANKER 

       550        560        570        580        590        600 
VGGRIYTYRF PQNQDKYQTV ELGAMRFPKI EIMDRLLNLD KPWSLFSKLE KAGHKIPTIP 

       610        620        630        640        650        660 
YHLTVDNNLV YYNGKRIFAN TLLNDDPLYF SDTHNGGPGT AVPDKYTYQP YGDLLDAVYK 

       670        680        690        700        710        720 
KFSDDLENDF ESGFETLLKS DNYSTRAYLF EKGPYPQSVV NYLETMDTGT GLYDMAFSET 

       730        740        750        760        770        780 
IMDYFDFSAG DEWLCIDGGT DIIVNSMVKT LRPGCIEQGK VVTKVSRVVG KSGDVSNLKV 

       790        800        810        820        830        840 
DFLDGTEGRL FKHVISTGTL ASLRRVDLSD LKLSHNKRTA IRSLHYDHSV KIALSFKSRW 

       850        860        870        880        890        900 
WEDSKFMNGK PMLGGKSSTD LPVRTIVYPS YGIGQPGVSG VLIVSYTWSL DASRIGSLVG 

       910        920        930        940        950        960 
DRPSEEVLIK LCMANLAEVH NVPVATLQQL FVDYKCWDWY NDDYSSGAFA LYSPSQFSQL 

       970        980        990       1000       1010       1020 
FPSLTKPSPD GRFHLAGEAT SVHHGWVIGS LNSAYRSVDH ILQVEGLDEL RAKLRLNWGF 

      1030       1040       1050       1060       1070       1080 
IDEVEDPQDD QQYVDPHHNL SGPNAKKFRS NVSQVAIQPR FKAPRNFKPR NTAASIGGLK 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]Bienvenut W.V., Ura S., Insall R.H.
Submitted (JUL-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 40-54; 445-468 AND 1050-1060, MASS SPECTROMETRY.
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000177 Genomic DNA. Translation: EAL61634.1.
RefSeqXP_635128.1. XM_630036.1.

3D structure databases

ProteinModelPortalQ54EW2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDBDRAFT_0183806.

Proteomic databases

PRIDEQ54EW2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0235252; DDB0235252; DDB_G0291301.
GeneID8628074.
KEGGddi:DDB_G0291301.

Organism-specific databases

dictyBaseDDB_G0291301.

Phylogenomic databases

eggNOGCOG0665.
InParanoidQ54EW2.
OMARTMYTED.
ProtClustDBCLSZ2429256.

Family and domain databases

InterProIPR002937. Amino_oxidase.
IPR006076. FAD-dep_OxRdtase.
[Graphical view]
PfamPF01593. Amino_oxidase. 1 hit.
PF01266. DAO. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameY9130_DICDI
AccessionPrimary (citable) accession number: Q54EW2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 24, 2005
Last modified: March 19, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase