SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q54EW2

- Y9130_DICDI

UniProt

Q54EW2 - Y9130_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Putative bifunctional amine oxidase DDB_G0291301
Gene
DDB_G0291301
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids. Metabolizes sarcosine, L-pipecolic acid and L-proline By similarity.

Catalytic activityi

Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2.
L-pipecolate + O2 = (S)-2,3,4,5-tetrahydropyridine-2-carboxylate + H2O2.
An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei535 – 5351FAD By similarity
Binding sitei544 – 5441FAD By similarity
Binding sitei886 – 8861Substrate By similarity
Binding sitei978 – 9781FAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 4031FAD Reviewed prediction
Add
BLAST
Nucleotide bindingi563 – 5642FAD By similarity
Nucleotide bindingi987 – 9904FAD By similarity

GO - Molecular functioni

  1. L-amino-acid oxidase activity Source: UniProtKB-EC
  2. L-pipecolate oxidase activity Source: UniProtKB-EC
  3. sarcosine oxidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_176494. Abnormal metabolism in phenylketonuria.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative bifunctional amine oxidase DDB_G0291301
Including the following 2 domains:
Putative sarcosine oxidase (EC:1.5.3.1, EC:1.5.3.7)
Short name:
PSO
Putative L-amino-acid oxidase (EC:1.4.3.2)
Gene namesi
ORF Names:DDB_G0291301
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291301.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei508 – 52821Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10801080Putative bifunctional amine oxidase DDB_G0291301
PRO_0000388375Add
BLAST

Proteomic databases

PRIDEiQ54EW2.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDBDRAFT_0183806.

Structurei

3D structure databases

ProteinModelPortaliQ54EW2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – ?450450Putative sarcosine oxidase
Add
BLAST
Regioni?450 – 1080631Putative L-amino-acid oxidase
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the MSOX/MTOX family.
In the C-terminal section; belongs to the flavin monoamine oxidase family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0665.
InParanoidiQ54EW2.
OMAiRTMYTED.
PhylomeDBiQ54EW2.

Family and domain databases

InterProiIPR002937. Amino_oxidase.
IPR006076. FAD-dep_OxRdtase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
PF01266. DAO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54EW2-1 [UniParc]FASTAAdd to Basket

« Hide

MREFLKDDYD VIVCGGGPVG LATAYRCAKA GKKVLCLEKS VFFNGGGSSG     50
DVVRMLRTMY TEDYMADLAH ETLGLWKELG DDAGEGDLVW MTGLLNFGDP 100
NYGAGGPEGT LLGPIPNLER LGMQYKVLTA QEIMEEYPFR NIPSNHQGVF 150
APDNGVINLP LVLRSLYKLC LQYGCKMVSH AEVKLIKNLS TTMVEIEVEH 200
TDVDQKNKQS FKVKSKKAAI TSNSFCNHII KPSFGWELDM TIWEMTSSYF 250
VAKPGPNATV FKSMWFNFQN DTDNDPTKSN LYYGFPAVPW MTDNHCRIAL 300
DAALRQIKDP NDRHDGVETH DVNRTRDWVR EHIPGVDDTP LFNVSALMAN 350
VYDNMFVLDF IPETNNNVVM FACGWAMKFI PLLGKILSQL LDEGKTQYPI 400
DHFALNRGNG ALIIKEGQTP KSVNSQVRAP RYTSMHCKPL TIAKSTVPTN 450
QSSNPDGASS TAPTQSLRSL FASRLLQRSV GMEAKFHSII AKNRSKRSTK 500
ASQKDLTVGI IGAGMAGLYA AMILQDLGLQ YNILEANKER VGGRIYTYRF 550
PQNQDKYQTV ELGAMRFPKI EIMDRLLNLD KPWSLFSKLE KAGHKIPTIP 600
YHLTVDNNLV YYNGKRIFAN TLLNDDPLYF SDTHNGGPGT AVPDKYTYQP 650
YGDLLDAVYK KFSDDLENDF ESGFETLLKS DNYSTRAYLF EKGPYPQSVV 700
NYLETMDTGT GLYDMAFSET IMDYFDFSAG DEWLCIDGGT DIIVNSMVKT 750
LRPGCIEQGK VVTKVSRVVG KSGDVSNLKV DFLDGTEGRL FKHVISTGTL 800
ASLRRVDLSD LKLSHNKRTA IRSLHYDHSV KIALSFKSRW WEDSKFMNGK 850
PMLGGKSSTD LPVRTIVYPS YGIGQPGVSG VLIVSYTWSL DASRIGSLVG 900
DRPSEEVLIK LCMANLAEVH NVPVATLQQL FVDYKCWDWY NDDYSSGAFA 950
LYSPSQFSQL FPSLTKPSPD GRFHLAGEAT SVHHGWVIGS LNSAYRSVDH 1000
ILQVEGLDEL RAKLRLNWGF IDEVEDPQDD QQYVDPHHNL SGPNAKKFRS 1050
NVSQVAIQPR FKAPRNFKPR NTAASIGGLK 1080
Length:1,080
Mass (Da):120,674
Last modified:May 24, 2005 - v1
Checksum:iFB0C9DA09FC71D6D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61634.1.
RefSeqiXP_635128.1. XM_630036.1.

Genome annotation databases

EnsemblProtistsiDDB0235252; DDB0235252; DDB_G0291301.
GeneIDi8628074.
KEGGiddi:DDB_G0291301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61634.1 .
RefSeqi XP_635128.1. XM_630036.1.

3D structure databases

ProteinModelPortali Q54EW2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDBDRAFT_0183806.

Proteomic databases

PRIDEi Q54EW2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0235252 ; DDB0235252 ; DDB_G0291301 .
GeneIDi 8628074.
KEGGi ddi:DDB_G0291301.

Organism-specific databases

dictyBasei DDB_G0291301.

Phylogenomic databases

eggNOGi COG0665.
InParanoidi Q54EW2.
OMAi RTMYTED.
PhylomeDBi Q54EW2.

Enzyme and pathway databases

Reactomei REACT_176494. Abnormal metabolism in phenylketonuria.

Family and domain databases

InterProi IPR002937. Amino_oxidase.
IPR006076. FAD-dep_OxRdtase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
PF01266. DAO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Ura S., Insall R.H.
    Submitted (JUL-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 40-54; 445-468 AND 1050-1060, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: AX2.

Entry informationi

Entry nameiY9130_DICDI
AccessioniPrimary (citable) accession number: Q54EW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Multifunctional enzyme, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi