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Protein

Leucine--tRNA ligase, mitochondrial

Gene

mleuS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei641ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
LeuRM
Leucyl-tRNA synthetase
Gene namesi
Name:mleuS
ORF Names:DDB_G0291346
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291346. mleuS.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000328292? – 940Leucine--tRNA ligase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

PaxDbiQ54ET5.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231251.

Structurei

3D structure databases

ProteinModelPortaliQ54ET5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi54 – 64"HIGH" regionBy similarityAdd BLAST11
Motifi638 – 642"KMSKS" regionBy similarity5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0435. Eukaryota.
COG0495. LUCA.
InParanoidiQ54ET5.
KOiK01869.
OMAiFLLLHEH.
PhylomeDBiQ54ET5.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 6 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54ET5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNLYRSSLK NLKLPNINNN IKSNLVIRSY TTNINGNIKN DDDNNKFYSL
60 70 80 90 100
SQFPYPSGAL HMGHVRVYTI SDCIARLKRM QGYDVIHPMG WDAFGLPAEN
110 120 130 140 150
AAIDKQVSPS EWTNLNISSM RDQLKLLNFQ FDWDRELSTC NKEYYRWTQE
160 170 180 190 200
IFLRLLKSGL AYRKSATVNW DPIDQTVLAN EQVDAQGRSW RSNAIVEKKE
210 220 230 240 250
MKQWFYKITS MADRLTDDLD QLPGWSDEIK NMQKEWIGRS YGHLIEFQSC
260 270 280 290 300
AQKPLSNITV FTTRAETIYG VSFLAISPHH SEINQIRANL INDEKRLELD
310 320 330 340 350
QYLKEIQEIK NKMGTQEDVE NLKTFNTGLT FYQPITKKYI PLILSNFVHA
360 370 380 390 400
DYGTGAVMGV PSHNRSDYQV AKQQNLKLLP VLGIEREQQQ QQQQQQQQQQ
410 420 430 440 450
LEIEEECYDY SNTGKLINSG QDTGIEFKEF IKRLEDQQLI KRQTNYRIHD
460 470 480 490 500
WLISRQRYWG TPIPIIVCEK CGDVPVPSDQ LPVELPIDIQ FTGKGNLLNQ
510 520 530 540 550
LDHWKNVKCP CCGSQATRET DTMDTFVDSS WYFLRFLDSK NSQSIFSSEL
560 570 580 590 600
VNRFMPIDVY VGGIEHAILH LLYSRFITKF LKDQQLIDHS EPFKVLLAQG
610 620 630 640 650
LVKSPTYRDS ITNKPIHPSN VEFKTIKSNE SGKSQQQTIN KLTGNQVSVT
660 670 680 690 700
IEKMSKSKLN GIDPKEIIDK YGSDTLKTYI LFKAPPEKSL DWDTQGIEGC
710 720 730 740 750
KKWLTRINVS IQSFLNQFDV IEGKEQHQHQ QQQHQQPLPS SEFNEQQSKE
760 770 780 790 800
VKDILFETHL TMNKVTESID KHSFNTGIAA LMELSNTLQK SSPQIKLTKE
810 820 830 840 850
YYQSLRALTL MLFPFSPIFS QIHWKSLIDD LPQSCKSFYS ENYSSFEQQS
860 870 880 890 900
YGNSNDNDVF NQRWPKPTPS ALVRDFNSLV IQFDGKTKGV ESIPTSITDF
910 920 930 940
SNFVQSNSKY LNRFKDKTID QIFIGTTKTG NSINFTFKKK
Length:940
Mass (Da):108,734
Last modified:May 24, 2005 - v1
Checksum:i275CCEC592792E38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61657.1.
RefSeqiXP_635155.1. XM_630063.1.

Genome annotation databases

EnsemblProtistsiEAL61657; EAL61657; DDB_G0291346.
GeneIDi8628101.
KEGGiddi:DDB_G0291346.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000177 Genomic DNA. Translation: EAL61657.1.
RefSeqiXP_635155.1. XM_630063.1.

3D structure databases

ProteinModelPortaliQ54ET5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231251.

Proteomic databases

PaxDbiQ54ET5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL61657; EAL61657; DDB_G0291346.
GeneIDi8628101.
KEGGiddi:DDB_G0291346.

Organism-specific databases

dictyBaseiDDB_G0291346. mleuS.

Phylogenomic databases

eggNOGiKOG0435. Eukaryota.
COG0495. LUCA.
InParanoidiQ54ET5.
KOiK01869.
OMAiFLLLHEH.
PhylomeDBiQ54ET5.

Miscellaneous databases

PROiQ54ET5.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 6 hits.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 2 hits.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYLM_DICDI
AccessioniPrimary (citable) accession number: Q54ET5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 30, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.