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Q54ET5

- SYLM_DICDI

UniProt

Q54ET5 - SYLM_DICDI

Protein

Leucine--tRNA ligase, mitochondrial

Gene

mleuS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei641 – 6411ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: dictyBase
    4. mRNA binding Source: dictyBase

    GO - Biological processi

    1. Group I intron splicing Source: dictyBase
    2. leucyl-tRNA aminoacylation Source: dictyBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_221378. Mitochondrial tRNA aminoacylation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
    Alternative name(s):
    LeuRM
    Leucyl-tRNA synthetase
    Gene namesi
    Name:mleuS
    ORF Names:DDB_G0291346
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0291346. mleuS.

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: dictyBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 940Leucine--tRNA ligase, mitochondrialPRO_0000328292
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231251.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54ET5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi54 – 6411"HIGH" regionBy similarityAdd
    BLAST
    Motifi638 – 6425"KMSKS" regionBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0495.
    KOiK01869.
    OMAiMAFAGPP.
    PhylomeDBiQ54ET5.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54ET5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNLYRSSLK NLKLPNINNN IKSNLVIRSY TTNINGNIKN DDDNNKFYSL    50
    SQFPYPSGAL HMGHVRVYTI SDCIARLKRM QGYDVIHPMG WDAFGLPAEN 100
    AAIDKQVSPS EWTNLNISSM RDQLKLLNFQ FDWDRELSTC NKEYYRWTQE 150
    IFLRLLKSGL AYRKSATVNW DPIDQTVLAN EQVDAQGRSW RSNAIVEKKE 200
    MKQWFYKITS MADRLTDDLD QLPGWSDEIK NMQKEWIGRS YGHLIEFQSC 250
    AQKPLSNITV FTTRAETIYG VSFLAISPHH SEINQIRANL INDEKRLELD 300
    QYLKEIQEIK NKMGTQEDVE NLKTFNTGLT FYQPITKKYI PLILSNFVHA 350
    DYGTGAVMGV PSHNRSDYQV AKQQNLKLLP VLGIEREQQQ QQQQQQQQQQ 400
    LEIEEECYDY SNTGKLINSG QDTGIEFKEF IKRLEDQQLI KRQTNYRIHD 450
    WLISRQRYWG TPIPIIVCEK CGDVPVPSDQ LPVELPIDIQ FTGKGNLLNQ 500
    LDHWKNVKCP CCGSQATRET DTMDTFVDSS WYFLRFLDSK NSQSIFSSEL 550
    VNRFMPIDVY VGGIEHAILH LLYSRFITKF LKDQQLIDHS EPFKVLLAQG 600
    LVKSPTYRDS ITNKPIHPSN VEFKTIKSNE SGKSQQQTIN KLTGNQVSVT 650
    IEKMSKSKLN GIDPKEIIDK YGSDTLKTYI LFKAPPEKSL DWDTQGIEGC 700
    KKWLTRINVS IQSFLNQFDV IEGKEQHQHQ QQQHQQPLPS SEFNEQQSKE 750
    VKDILFETHL TMNKVTESID KHSFNTGIAA LMELSNTLQK SSPQIKLTKE 800
    YYQSLRALTL MLFPFSPIFS QIHWKSLIDD LPQSCKSFYS ENYSSFEQQS 850
    YGNSNDNDVF NQRWPKPTPS ALVRDFNSLV IQFDGKTKGV ESIPTSITDF 900
    SNFVQSNSKY LNRFKDKTID QIFIGTTKTG NSINFTFKKK 940
    Length:940
    Mass (Da):108,734
    Last modified:May 24, 2005 - v1
    Checksum:i275CCEC592792E38
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000177 Genomic DNA. Translation: EAL61657.1.
    RefSeqiXP_635155.1. XM_630063.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231251; DDB0231251; DDB_G0291346.
    GeneIDi8628101.
    KEGGiddi:DDB_G0291346.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000177 Genomic DNA. Translation: EAL61657.1 .
    RefSeqi XP_635155.1. XM_630063.1.

    3D structure databases

    ProteinModelPortali Q54ET5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231251.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231251 ; DDB0231251 ; DDB_G0291346 .
    GeneIDi 8628101.
    KEGGi ddi:DDB_G0291346.

    Organism-specific databases

    dictyBasei DDB_G0291346. mleuS.

    Phylogenomic databases

    eggNOGi COG0495.
    KOi K01869.
    OMAi MAFAGPP.
    PhylomeDBi Q54ET5.

    Enzyme and pathway databases

    Reactomei REACT_221378. Mitochondrial tRNA aminoacylation.

    Miscellaneous databases

    PROi Q54ET5.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 2 hits.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiSYLM_DICDI
    AccessioniPrimary (citable) accession number: Q54ET5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3