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Q54E83 (GSHB_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutathione synthetase
Short name=GSH synthetase
Short name=GSH-S
EC=6.3.2.3
Alternative name(s):
Glutathione synthase
Gene names
Name:gshB
ORF Names:DDB_G0291756
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length476 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the eukaryotic GSH synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 476476Glutathione synthetase
PRO_0000312710

Regions

Nucleotide binding367 – 37610ATP By similarity
Nucleotide binding400 – 4034ATP By similarity
Region141 – 1444Substrate binding By similarity
Region211 – 2133Substrate binding By similarity
Region267 – 2704Substrate binding By similarity
Region463 – 4642Substrate binding By similarity

Sites

Metal binding1371Magnesium By similarity
Metal binding1391Magnesium By similarity
Metal binding3711Magnesium By similarity
Binding site1171Substrate By similarity
Binding site1371ATP By similarity
Binding site2171Substrate By similarity
Binding site3081ATP By similarity
Binding site3781ATP By similarity
Binding site4261ATP By similarity
Binding site4521Substrate By similarity
Binding site4541ATP By similarity
Binding site4601ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54E83 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: 539645AD00EA4904

FASTA47654,140
        10         20         30         40         50         60 
MEEKLNDLKE QGIDWAFANG LIMIKKPTEE EAKNNVVNVT HVPFSLYPSK MNKKLFNEAC 

        70         80         90        100        110        120 
KLAEDYNLLV HNISKDYDFL QNTLKDVFDD FTQMLLNIQR KVVKEGIKQK ISLGIFRSDY 

       130        140        150        160        170        180 
MFHNKIEGEE ERIYQVELNT ISSSLAVVSN RVFNLHKYLI GRNDLNDNGY DLLNHPTNQS 

       190        200        210        220        230        240 
DKEISDSIAL AHKLYNKEKS SVVLMIIQEG ERNIYDQKGL EFQLWSNHSI KLIRRTMKEI 

       250        260        270        280        290        300 
NQCAKLDEEN GSVLIVDGME ISVAYYRAGY TPNDYTSSGG DEWKARLLIE RSLAIKCPTI 

       310        320        330        340        350        360 
AHHLVGVKKI QQVLAQPGVL EKFINNDKES LQRVKRSFTG LYSLSKEDID MSVVKEAIES 

       370        380        390        400        410        420 
PQNYVMKPQR EGGGNNIYND QVAIALKSMS SEELSSYILM DKIMSKSFKT HVVRDRQLLE 

       430        440        450        460        470 
IEGLYELGIY SVFISNGDDD IVLNKQAGIL LRTKTANSDE VGVAAGFGLL DSPILE

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AAFI02000182 Genomic DNA. Translation: EAL61574.1.
RefSeqXP_629979.1. XM_629977.1.

3D structure databases

ProteinModelPortalQ54E83.
ModBaseSearch...

Protein-protein interaction databases

STRING44689.DDB_0231404.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231404; DDB0231404; DDB_G0291756.
GeneID8628309.
KEGGddi:DDB_G0291756.

Organism-specific databases

dictyBaseDDB_G0291756. gshB.

Phylogenomic databases

eggNOGNOG329040.
KOK01920.
OMATKSSEHA.
ProtClustDBCLSZ2429522.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR11130. PTHR11130. 1 hit.
PfamPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF001558. GSHase. 1 hit.
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01986. glut_syn_euk. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGSHB_DICDI
AccessionPrimary (citable) accession number: Q54E83
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 24, 2005
Last modified: May 29, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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