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Protein

Glutathione synthetase

Gene

gshB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamate--cysteine ligase (gcsA)
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei117SubstrateBy similarity1
Metal bindingi137MagnesiumBy similarity1
Binding sitei137ATPBy similarity1
Metal bindingi139MagnesiumBy similarity1
Binding sitei217SubstrateBy similarity1
Binding sitei308ATPBy similarity1
Metal bindingi371MagnesiumBy similarity1
Binding sitei378ATPBy similarity1
Binding sitei426ATPBy similarity1
Binding sitei452SubstrateBy similarity1
Binding sitei454ATPBy similarity1
Binding sitei460ATP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi367 – 376ATPBy similarity10
Nucleotide bindingi400 – 403ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DDI-174403. Glutathione synthesis and recycling.
UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:gshB
ORF Names:DDB_G0291756
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291756. gshB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003127101 – 476Glutathione synthetaseAdd BLAST476

Proteomic databases

PaxDbiQ54E83.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi44689.DDB0231404.

Structurei

3D structure databases

ProteinModelPortaliQ54E83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 144Substrate bindingBy similarity4
Regioni211 – 213Substrate bindingBy similarity3
Regioni267 – 270Substrate bindingBy similarity4
Regioni463 – 464Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
InParanoidiQ54E83.
KOiK01920.
OMAiAYILMEI.
PhylomeDBiQ54E83.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54E83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKLNDLKE QGIDWAFANG LIMIKKPTEE EAKNNVVNVT HVPFSLYPSK
60 70 80 90 100
MNKKLFNEAC KLAEDYNLLV HNISKDYDFL QNTLKDVFDD FTQMLLNIQR
110 120 130 140 150
KVVKEGIKQK ISLGIFRSDY MFHNKIEGEE ERIYQVELNT ISSSLAVVSN
160 170 180 190 200
RVFNLHKYLI GRNDLNDNGY DLLNHPTNQS DKEISDSIAL AHKLYNKEKS
210 220 230 240 250
SVVLMIIQEG ERNIYDQKGL EFQLWSNHSI KLIRRTMKEI NQCAKLDEEN
260 270 280 290 300
GSVLIVDGME ISVAYYRAGY TPNDYTSSGG DEWKARLLIE RSLAIKCPTI
310 320 330 340 350
AHHLVGVKKI QQVLAQPGVL EKFINNDKES LQRVKRSFTG LYSLSKEDID
360 370 380 390 400
MSVVKEAIES PQNYVMKPQR EGGGNNIYND QVAIALKSMS SEELSSYILM
410 420 430 440 450
DKIMSKSFKT HVVRDRQLLE IEGLYELGIY SVFISNGDDD IVLNKQAGIL
460 470
LRTKTANSDE VGVAAGFGLL DSPILE
Length:476
Mass (Da):54,140
Last modified:May 24, 2005 - v1
Checksum:i539645AD00EA4904
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000182 Genomic DNA. Translation: EAL61574.1.
RefSeqiXP_629979.1. XM_629977.1.

Genome annotation databases

EnsemblProtistsiEAL61574; EAL61574; DDB_G0291756.
GeneIDi8628309.
KEGGiddi:DDB_G0291756.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000182 Genomic DNA. Translation: EAL61574.1.
RefSeqiXP_629979.1. XM_629977.1.

3D structure databases

ProteinModelPortaliQ54E83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0231404.

Proteomic databases

PaxDbiQ54E83.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL61574; EAL61574; DDB_G0291756.
GeneIDi8628309.
KEGGiddi:DDB_G0291756.

Organism-specific databases

dictyBaseiDDB_G0291756. gshB.

Phylogenomic databases

eggNOGiKOG0021. Eukaryota.
ENOG410XPHH. LUCA.
InParanoidiQ54E83.
KOiK01920.
OMAiAYILMEI.
PhylomeDBiQ54E83.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.
ReactomeiR-DDI-174403. Glutathione synthesis and recycling.

Miscellaneous databases

PROiQ54E83.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGSHB_DICDI
AccessioniPrimary (citable) accession number: Q54E83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 24, 2005
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.