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Protein

Glutathione synthetase

Gene

gshB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171SubstrateBy similarity
Metal bindingi137 – 1371MagnesiumBy similarity
Binding sitei137 – 1371ATPBy similarity
Metal bindingi139 – 1391MagnesiumBy similarity
Binding sitei217 – 2171SubstrateBy similarity
Binding sitei308 – 3081ATPBy similarity
Metal bindingi371 – 3711MagnesiumBy similarity
Binding sitei378 – 3781ATPBy similarity
Binding sitei426 – 4261ATPBy similarity
Binding sitei452 – 4521SubstrateBy similarity
Binding sitei454 – 4541ATPBy similarity
Binding sitei460 – 4601ATP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi367 – 37610ATPBy similarity
Nucleotide bindingi400 – 4034ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. glutathione binding Source: UniProtKB
  3. glutathione synthase activity Source: dictyBase
  4. magnesium ion binding Source: UniProtKB
  5. protein homodimerization activity Source: UniProtKB

GO - Biological processi

  1. glutathione biosynthetic process Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetase (EC:6.3.2.3)
Short name:
GSH synthetase
Short name:
GSH-S
Alternative name(s):
Glutathione synthase
Gene namesi
Name:gshB
ORF Names:DDB_G0291756
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291756. gshB.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Glutathione synthetasePRO_0000312710Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB_0231404.

Structurei

3D structure databases

ProteinModelPortaliQ54E83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni141 – 1444Substrate bindingBy similarity
Regioni211 – 2133Substrate bindingBy similarity
Regioni267 – 2704Substrate bindingBy similarity
Regioni463 – 4642Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.Curated

Phylogenomic databases

eggNOGiNOG329040.
InParanoidiQ54E83.
KOiK01920.
OMAiVFDQRHI.
PhylomeDBiQ54E83.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54E83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKLNDLKE QGIDWAFANG LIMIKKPTEE EAKNNVVNVT HVPFSLYPSK
60 70 80 90 100
MNKKLFNEAC KLAEDYNLLV HNISKDYDFL QNTLKDVFDD FTQMLLNIQR
110 120 130 140 150
KVVKEGIKQK ISLGIFRSDY MFHNKIEGEE ERIYQVELNT ISSSLAVVSN
160 170 180 190 200
RVFNLHKYLI GRNDLNDNGY DLLNHPTNQS DKEISDSIAL AHKLYNKEKS
210 220 230 240 250
SVVLMIIQEG ERNIYDQKGL EFQLWSNHSI KLIRRTMKEI NQCAKLDEEN
260 270 280 290 300
GSVLIVDGME ISVAYYRAGY TPNDYTSSGG DEWKARLLIE RSLAIKCPTI
310 320 330 340 350
AHHLVGVKKI QQVLAQPGVL EKFINNDKES LQRVKRSFTG LYSLSKEDID
360 370 380 390 400
MSVVKEAIES PQNYVMKPQR EGGGNNIYND QVAIALKSMS SEELSSYILM
410 420 430 440 450
DKIMSKSFKT HVVRDRQLLE IEGLYELGIY SVFISNGDDD IVLNKQAGIL
460 470
LRTKTANSDE VGVAAGFGLL DSPILE
Length:476
Mass (Da):54,140
Last modified:May 24, 2005 - v1
Checksum:i539645AD00EA4904
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000182 Genomic DNA. Translation: EAL61574.1.
RefSeqiXP_629979.1. XM_629977.1.

Genome annotation databases

EnsemblProtistsiDDB0231404; DDB0231404; DDB_G0291756.
GeneIDi8628309.
KEGGiddi:DDB_G0291756.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000182 Genomic DNA. Translation: EAL61574.1.
RefSeqiXP_629979.1. XM_629977.1.

3D structure databases

ProteinModelPortaliQ54E83.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0231404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231404; DDB0231404; DDB_G0291756.
GeneIDi8628309.
KEGGiddi:DDB_G0291756.

Organism-specific databases

dictyBaseiDDB_G0291756. gshB.

Phylogenomic databases

eggNOGiNOG329040.
InParanoidiQ54E83.
KOiK01920.
OMAiVFDQRHI.
PhylomeDBiQ54E83.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210.

Miscellaneous databases

PROiQ54E83.

Family and domain databases

Gene3Di1.10.1080.10. 1 hit.
3.30.1490.50. 1 hit.
3.30.1490.80. 1 hit.
3.40.50.1760. 1 hit.
InterProiIPR004887. Glutathione_synth_subst-bd_euk.
IPR014042. Glutathione_synthase_a-hlx_euk.
IPR014709. Glutathione_synthase_dom.
IPR005615. Glutathione_synthase_euk.
IPR014049. Glutathione_synthase_N_euk.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR11130. PTHR11130. 1 hit.
PfamiPF03917. GSH_synth_ATP. 1 hit.
PF03199. GSH_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF001558. GSHase. 1 hit.
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01986. glut_syn_euk. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiGSHB_DICDI
AccessioniPrimary (citable) accession number: Q54E83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 24, 2005
Last modified: January 7, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.