Reviewed, UniProtKB/Swiss-Prot Q54E62 (OST48_DICDI)

Names and origin

Protein names

Recommended name:
    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit homolog
      Short name=Oligosaccharyl transferase 48 kDa subunit homolog
      Short name=DDOST 48 kDa subunit homolog
    EC=2.4.1.119

Gene names

Name:	ddost
ORF Names:	DDB_G0291780

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains By similarity.
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 426	404	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit homolog		PRO_0000328637
Regions
	Topological domain	23 – 398	376	Lumenal Potential
	Transmembrane	399 – 419	21	Potential
	Topological domain	420 – 426	7	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	286	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54E62-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: C14040D8C68810C7	FASTA	426	47,889
	10 20 30 40 50 60 MMMKSILITF IIASALLSSV FADIGGKRTL VVLDDLSIKK THSTFFKNLE NKGYKLQFEQ 70 80 90 100 110 120 SNTKVVLEKY GDFNFDNLIL FSPTSESLSF SSADVTRFID GGNNVLFAGS NVISENIRDI 130 140 150 160 170 180 AAECGMEIEE DKTLIFDHFN YDKSQSDHSV LVADQFIDDS PIILQGLNKP ILFKGIGHKI 190 200 210 220 230 240 RNNPLNYAIL TGSSTAFSAK AISGVSTKLM GKSCGLVSSL QARNNARVTF SGSLDLFSDK 250 260 270 280 290 300 SFYSKIDNKE SGNKEFVERL VSWTFQERGI LRASELELVK ISTESNSTVA PDVFTIKDEV 310 320 330 340 350 360 KYSLKVEEFD GIKGKWVPYV GSLQLEVIML DPYIRTFIKG DANGLYKIHF KLPDVYGVFT 370 380 390 400 410 420 FEASIHKSGY STLDHIYRKP ILPFRHDSYE RFIPAAFPYY ATCFSMLIGT FIFSIIFLFN KDQLNK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000184 Genomic DNA. Translation: EAL61549.1.
RefSeq	XP_629963.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54E62.
Genome annotation databases
GeneID	3386626.
GenomeReviews	Gene locus ddost in contig CM000155_GR.
KEGG	ddi:DDB_0233148.
Organism-specific databases
dictyBase	DDB_G0291780. ddost.
Phylogenomic databases
OMA	VQLEFVR.
Enzyme and pathway databases
BRENDA	2.4.1.119. 424.
Family and domain databases
InterPro	IPR005013. DDOST_48kDa. [Graphical view]
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_DICDI

Accession

Primary (citable) accession number: Q54E62

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)