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Protein

Hydroxyacid oxidase

Gene

haox

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Has 2-hydroxyacid oxidase activity.By similarity

Catalytic activityi

(S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2.

Cofactori

FMNPROSITE-ProRule annotation

Pathwayi: glycolate degradation

This protein is involved in step 1 of the subpathway that synthesizes 3-phospho-D-glycerate from glycolate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Hydroxyacid oxidase (haox)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolate degradation, which is itself part of Organic acid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 3-phospho-D-glycerate from glycolate, the pathway glycolate degradation and in Organic acid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52SubstratePROSITE-ProRule annotation1
Binding sitei134FMNPROSITE-ProRule annotation1
Binding sitei156FMNPROSITE-ProRule annotation1
Binding sitei158SubstratePROSITE-ProRule annotation1
Binding sitei184FMNPROSITE-ProRule annotation1
Binding sitei193SubstratePROSITE-ProRule annotation1
Binding sitei255FMNPROSITE-ProRule annotation1
Active sitei279Proton acceptorPROSITE-ProRule annotation1
Binding sitei282SubstratePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi310 – 334FMNPROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

ReactomeiR-DDI-389661. Glyoxylate metabolism and glycine degradation.
R-DDI-390918. Peroxisomal lipid metabolism.
UniPathwayiUPA00864; UER00830.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacid oxidase (EC:1.1.3.15)
Short name:
HAOX
Alternative name(s):
Glycolate oxidase
Short name:
GOX
Gene namesi
Name:haox
ORF Names:DDB_G0291814
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0291814. hao.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003282601 – 388Hydroxyacid oxidaseAdd BLAST388

Proteomic databases

PaxDbiQ54E41.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0267054.

Structurei

3D structure databases

ProteinModelPortaliQ54E41.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 384FMN hydroxy acid dehydrogenasePROSITE-ProRule annotationAdd BLAST359

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 10Poly-Asn4

Sequence similaritiesi

Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family.PROSITE-ProRule annotation
Contains 1 FMN hydroxy acid dehydrogenase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
InParanoidiQ54E41.
KOiK11517.
OMAiTEYQEIA.
PhylomeDBiQ54E41.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54E41-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENQFKNNNN SSSIETSNQF SNKKTNRLDS FVSVSELHEE AKRLLPKMAY
60 70 80 90 100
DYYASGSNDQ ITLAENENAF SRIKLVPRSL VDVSKVNTKT RIFGRDISTP
110 120 130 140 150
ILIAPWAMQR MASQRGELDT VEASKEFNTI MTLSSLSTTS VEDLSSATNG
160 170 180 190 200
NPGWFQLYVF KDRKVSEELV KRAESIGYSA LVLTVDTPFL GKRTADFKNS
210 220 230 240 250
FKLPNGLSLK IFEKLMLSNL DGGLNQYIAT MIDPSLTWND LKWLKSITKL
260 270 280 290 300
PILVKGIMCP KDAELALQYG ADGIIVSNHG GRQLDTCPST IEVLPYISKV
310 320 330 340 350
VRGRVPLILD GGIRRGTDVL KALAFGANAV CIGRPIIWGL STGGKDGVLK
360 370 380
VLNLLNSELQ LAMALTGITN ISDINNSIIW DQNKYIKL
Length:388
Mass (Da):42,877
Last modified:May 24, 2005 - v1
Checksum:iC42C59FFE992395D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000185 Genomic DNA. Translation: EAL61528.1.
RefSeqiXP_629946.1. XM_629944.1.

Genome annotation databases

EnsemblProtistsiEAL61528; EAL61528; DDB_G0291814.
GeneIDi8628352.
KEGGiddi:DDB_G0291814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000185 Genomic DNA. Translation: EAL61528.1.
RefSeqiXP_629946.1. XM_629944.1.

3D structure databases

ProteinModelPortaliQ54E41.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0267054.

Proteomic databases

PaxDbiQ54E41.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiEAL61528; EAL61528; DDB_G0291814.
GeneIDi8628352.
KEGGiddi:DDB_G0291814.

Organism-specific databases

dictyBaseiDDB_G0291814. hao.

Phylogenomic databases

eggNOGiKOG0538. Eukaryota.
COG1304. LUCA.
InParanoidiQ54E41.
KOiK11517.
OMAiTEYQEIA.
PhylomeDBiQ54E41.

Enzyme and pathway databases

UniPathwayiUPA00864; UER00830.
ReactomeiR-DDI-389661. Glyoxylate metabolism and glycine degradation.
R-DDI-390918. Peroxisomal lipid metabolism.

Miscellaneous databases

PROiQ54E41.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR012133. Alpha-hydoxy_acid_DH_FMN.
IPR000262. FMN-dep_DH.
IPR008259. FMN_hydac_DH_AS.
[Graphical view]
PfamiPF01070. FMN_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITEiPS00557. FMN_HYDROXY_ACID_DH_1. 1 hit.
PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHAOX_DICDI
AccessioniPrimary (citable) accession number: Q54E41
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: November 2, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.