Q54DY1 (ARGJ_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine biosynthesis bifunctional protein ArgJ, mitochondrial

Cleaved into the following 2 chains:

1. Arginine biosynthesis bifunctional protein ArgJ alpha chain
2. Arginine biosynthesis bifunctional protein ArgJ beta chain

Including the following 2 domains:

1. Glutamate N-acetyltransferase
   Short name=GAT
   EC=2.3.1.35
   Alternative name(s):
   Ornithine acetyltransferase
   Short name=OATase
   Ornithine transacetylase
2. Amino-acid acetyltransferase
   EC=2.3.1.1
   Alternative name(s):
   N-acetylglutamate synthase
   Short name=AGS

Gene names

Name:	argJ
Synonyms:	arg7
ORF Names:	DDB_G0291916

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of acetylglutamate from glutamate and acetyl-CoA, and of ornithine by transacetylation between acetylornithine and glutamate By similarity.
Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.
Subunit structure	Heterodimer of an alpha and a beta chain By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Post-translational modification	The alpha and beta chains are autoproteolytically processed from a single precursor protein within the mitochondrion By similarity.
Sequence similarities	Belongs to the ArgJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
PTM	Autocatalytic cleavage
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase ornithine biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	mitochondrial matrix Inferred from sequence or structural similarity. Source: dictyBase
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase glutamate N-acetyltransferase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 23	23	Mitochondrion Potential
	Chain	24 – 209	186	Arginine biosynthesis bifunctional protein ArgJ alpha chain By similarity		PRO_0000327743
	Chain	210 – 442	233	Arginine biosynthesis bifunctional protein ArgJ beta chain By similarity		PRO_0000327744
Sites
	Site	209 – 210	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54DY1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 73D3392FBA7E8847	FASTA	442	47,724
	10 20 30 40 50 60 MFSNIVNKKL FQNSTTTSFL RHYSTIKNTT TSPKGFKTGT YACGLKKSGA LDICLIHSEK 70 80 90 100 110 120 ECNVAAVFTE NKVKAAPVLL SKSLIEKHKN TGFQSLIINS GGANACTGPE GEKNAQTMSF 130 140 150 160 170 180 LSSKLVNAKQ QSLVMSTGII GQQLDMKKVE LGITKASENL KDDWLSAAKA IMTTDKVPKL 190 200 210 220 230 240 VQKQIEIQGK QVNITGICKG AGMIHPNMAT MLCTLCTDAD ISEEALKSAL KHSIDYSFNS 250 260 270 280 290 300 INVDGDMSTN DTVAVFSNGL AGNKRIDSTN SKEYLLLEKA MRECATELAQ MIVRDAEGAT 310 320 330 340 350 360 KFISVVVRGA KTEKDGKIIA NSIATSSLVK TAMYGEDANW GRVLAAVGYS GADGVVPSNI 370 380 390 400 410 420 SMWFASGYGD NIGIGQKNDP LIAMQFLKDG QPTPKDDEKA AKLLSNKDIS IIVDLNNGNQ 430 440 NYTMWTCDLT VDYVKANSHY RT

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000186 Genomic DNA. Translation: EAL61488.1.
RefSeq	XP_629915.1. XM_629913.1.
3D structure databases
HSSP	HSSP built from PDB template 1VZ7 based on UniProtKB Q53940.
ProteinModelPortal	Q54DY1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54DY1.
Protein family/group databases
MEROPS	T05.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231449; DDB0231449; DDB_G0291916.
GeneID	8628416.
GenomeReviews	Gene locus argJ in contig CM000155_GR.
KEGG	ddi:DDB_G0291916.
Organism-specific databases
dictyBase	DDB_G0291916. argJ.
Phylogenomic databases
eggNOG	KOG2786.
GeneTree	EPrGT00050000005707.
HOGENOM	HBG284202.
OMA	GRDPNWG.
PhylomeDB	Q54DY1.
Family and domain databases
InterPro	IPR002813. Arg_biosynth_ArgJ. IPR016117. Pept_S58_DmpA/Arg_biosyn_ArgJ. [Graphical view]
KO	K00620.
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. Arg_biosynth_ArgJ. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF56266. Pept_S58_DmpA/Arg_biosyn_ArgJ. 1 hit.
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_DICDI

Accession

Primary (citable) accession number: Q54DY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)