Reviewed, UniProtKB/Swiss-Prot Q54DX7 (TAZ1_DICDI)

Names and origin

Protein names

Recommended name:
    Putative lysophosphatidylcholine acyltransferase
    EC=2.3.1.23
Alternative name(s):
    1-acylglycerophosphocholine O-acyltransferase
    Tafazzin homolog

Gene names

Name:	taz
ORF Names:	DDB_G0291922

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Acyltransferase which mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC). Required for normal phospholipid content of mitochondrial membranes. Involved in the remodeling of the acyl groups of cardiolipin in the mitochondrial inner membrane, which affects the assembly and stability of respiratory chain complex IV and its supercomplex forms By similarity.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycero-3-phosphocholine = CoA + 1,2-diacyl-sn-glycero-3-phosphocholine.
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein; Intermembrane side Potential.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphocholine By similarity.
Sequence similarities	Belongs to the taffazin family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-acylglycerophosphocholine O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Putative lysophosphatidylcholine acyltransferase		PRO_0000328231
Regions
	Transmembrane	24 – 42	19	Potential
	Motif	74 – 80	7	HXXXXD motif By similarity
	Compositional bias	4 – 12	9	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q54DX7-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 8CE1C94790B76524	FASTA	285	32,784
	10 20 30 40 50 60 MDSNNSNNNN KNLKQICDIP KPQFLSKGVF TLVGVLCKFW ISMNTVTTSG IDKLVNEIDK 70 80 90 100 110 120 THQLKRPMIT IANHSSNLDD PLLWGVLPNR ILMDPSKQRW TLGASNILFT NWFYSKFFSL 130 140 150 160 170 180 GKCIKIVRGD GIYQDGMNES IDRLSEGQWL HIFPEGRISQ QTQLLYFKWG LGRLVGECYR 190 200 210 220 230 240 RTGVVPLVVP IYHQGMEKSM PLAKLPIPRV GINLDIKVGD NIYCDQVISK YIDDNKISDL 250 260 270 280 TDYLSQDDKK RKDFYKTITL HIEDEYQKII PPTNRGRFSH PTIKD

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000186 Genomic DNA. Translation: EAL61491.1.
RefSeq	XP_629919.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3386571.
GenomeReviews	Gene locus taz in contig CM000155_GR.
KEGG	ddi:DDB_0237792.
Organism-specific databases
dictyBase	DDB_G0291922. taz.
Phylogenomic databases
OMA	ITIANHS.
Enzyme and pathway databases
BRENDA	2.3.1.23. 424.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR000872. Tafazzin. [Graphical view]
PANTHER	PTHR12497. Tafazzin. 1 hit.
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PRINTS	PR00979. TAFAZZIN.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TAZ1_DICDI

Accession

Primary (citable) accession number: Q54DX7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)