Q54DT8 (PPOX_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protoporphyrinogen oxidase
Short name=PPO
EC=1.3.3.4

Gene names

Name:	ppox
Synonyms:	hemG
ORF Names:	DDB_G0292040

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX By similarity.
Catalytic activity	Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.
Cofactor	FAD By similarity.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrin-IX from protoporphyrinogen-IX: step 1/1.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the protoporphyrinogen oxidase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular component movement Inferred from genetic interaction PubMed 18164290. Source: dictyBase heme biosynthetic process Inferred from sequence or structural similarity. Source: dictyBase protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro oxygen-dependent protoporphyrinogen oxidase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 532	532	Protoporphyrinogen oxidase		PRO_0000327793
Regions
	Nucleotide binding	9 – 14	6	FAD Potential
	Compositional bias	397 – 410	14	Poly-Asn

Sequences

	Sequence		Length	Mass (Da)
	Q54DT8 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: EBB0066495A696A7	FASTA	532	59,681
	10 20 30 40 50 60 MIQKVGIIGS GISGLSSYYY LRNGINLTSK FSKNNLKINI FEKSNKVGGN IQTRIIQGKN 70 80 90 100 110 120 KDEKIIVEEG PRSLRALGRG LNTLEFIKRL GISNDIIFSS ANSNGKFVLL DGKPKEIPMT 130 140 150 160 170 180 SLFDIIKFSF KHSIVSSILK EPFKKVPSQV KEMDPNWDES VHDFFSRRLG KTMTKTFIEP 190 200 210 220 230 240 TILGIYGGDY TNLSIKSTFK RAALLEPFGG LILGSLFKSK KQKQFELDLD KNEKRLLPSK 250 260 270 280 290 300 NELTELFDKD TDKTNVFSFK ENGLSRMIQK LKSLIESDSL TKLYLSTSIV EIEKDVTNGT 310 320 330 340 350 360 LKVTDNKGNQ YQYDQLISTI PLNQLAPMFK KSDSKLYQLL QSVNYTSIAV INLIYKSNKN 370 380 390 400 410 420 VVKIISDKGF GYLVPSKENQ SVIGVCFDSN TFPEFVNNNN NNNNDNDNGN EKDQSIITVM 430 440 450 460 470 480 IGGNNGIKDR NDNWIDVTNT SKDKLLDIAL KHLDKVLDIE SSPDFTNVSI YDNGIPHYNI 490 500 510 520 530 GHQNLINEIQ NHITKNYGTT LLLGGNSIDG VGINDSIHKS KQLINSLKLS NN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000187 Genomic DNA. Translation: EAL61376.1.
RefSeq	XP_629784.1. XM_629782.1.
3D structure databases
HSSP	HSSP built from PDB template 1SEZ based on UniProtKB O24164.
ProteinModelPortal	Q54DT8.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231419.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231419; DDB0231419; DDB_G0292040.
GeneID	8628461.
KEGG	ddi:DDB_G0292040.
Organism-specific databases
dictyBase	DDB_G0292040. hemG.
Phylogenomic databases
eggNOG	COG1232.
KO	K00231.
OMA	HIRSTIN.
Enzyme and pathway databases
UniPathway	UPA00251; UER00324.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002937. Amino_oxidase. IPR016040. NAD(P)-bd_dom. IPR004572. Protoporphyrinogen_oxidase. [Graphical view]
Pfam	PF01593. Amino_oxidase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00562. proto_IX_ox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPOX_DICDI

Accession

Primary (citable) accession number: Q54DT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)