Q54DD3 (GCST_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aminomethyltransferase, mitochondrial
EC=2.1.2.10

Alternative name(s):
Glycine cleavage system T protein
Short name=GCVT

Gene names

Name:	gcvT
ORF Names:	DDB_G0292326

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	403 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity.
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the GcvT family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycine catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	glycine cleavage complex Inferred from sequence or structural similarity. Source: dictyBase mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: EC transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 25	25	Mitochondrion Potential
	Chain	26 – 403	378	Aminomethyltransferase, mitochondrial		PRO_0000327652

Sequences

	Sequence		Length	Mass (Da)
	Q54DD3 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: AF126F821C858B45	FASTA	403	44,247
	10 20 30 40 50 60 MISQNIIKII QKSNKRYFSS SNELKKTALN ELHKELGAKM VPFCGWEMPV QYPAGVMKEH 70 80 90 100 110 120 LHVRKESGLF DVSHMGQLRI HGKDRVKFFE SIVVADLQAL PTGHSKLSVF TNEKGGIIDD 130 140 150 160 170 180 TMITNAGDSL YVVVNAGCAD KDISHINEKI KEFKSVNPTH DVSMQLLEDL SLIAIQGPTT 190 200 210 220 230 240 ESILQKFVKD QDITNMEFMT QRPMTIAGID CIVTRCGYTG EDGFEISVPS KQAVRLAELF 250 260 270 280 290 300 LATSNASIES GIKPAGLGAR DSLRLEAGLC LYGHDLNDDI TPIEASLNWL ISKRRREEGG 310 320 330 340 350 360 FPGASIIQKQ LQKDGCPQKR VGVIINGAPA REGCLILDPS TNQEIGKVTS GTISPITRQS 370 380 390 400 ISMAYVKTPF SKIGTQVNVS IRGKPITATI SKMPFVPTNY KKL

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000189 Genomic DNA. Translation: EAL61287.1.
RefSeq	XP_629708.1. XM_629706.1.
3D structure databases
HSSP	HSSP built from PDB template 1WSR based on UniProtKB P48728.
ProteinModelPortal	Q54DD3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54DD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231218; DDB0231218; DDB_G0292326.
GeneID	8628622.
GenomeReviews	Gene locus gcvT in contig CM000155_GR.
KEGG	ddi:DDB_G0292326.
Organism-specific databases
dictyBase	DDB_G0292326. gcvT.
Phylogenomic databases
eggNOG	KOG2770.
GeneTree	EPrGT00050000002466.
HOGENOM	HBG299834.
OMA	RITRCGY.
PhylomeDB	Q54DD3.
ProtClustDB	CLSZ2429438.
Family and domain databases
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
KO	K00605.
PANTHER	PTHR13847:SF5. PTHR13847:SF5. 1 hit.
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. GcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_DICDI

Accession

Primary (citable) accession number: Q54DD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)