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Q54DD0

- AMPD_DICDI

UniProt

Q54DD0 - AMPD_DICDI

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Protein

AMP deaminase

Gene

amdA

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

AMP deaminase plays a critical role in energy metabolism.1 Publication

Catalytic activityi

AMP + H2O = IMP + NH3.

Cofactori

Zinc.Curated
Binds 1 zinc ion per subunit.By similarity

Enzyme regulationi

Activated by ATP, inhibited by GTP, EDTA and inorganic phosphate.

Kineticsi

  1. KM=1.6 mM for AMP1 Publication

Vmax=1.0 mmol/min/mg enzyme1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi221 – 2211Zinc; catalyticBy similarity
Metal bindingi223 – 2231Zinc; catalyticBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Metal bindingi488 – 4881Zinc; catalyticBy similarity
Binding sitei491 – 4911SubstrateBy similarity
Active sitei510 – 5101Proton acceptorPROSITE-ProRule annotation
Metal bindingi565 – 5651Zinc; catalyticBy similarity

GO - Molecular functioni

  1. AMP deaminase activity Source: dictyBase
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. multicellular organismal development Source: UniProtKB-KW
  3. purine ribonucleoside monophosphate metabolic process Source: dictyBase
  4. sporulation resulting in formation of a cellular spore Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase

Keywords - Biological processi

Differentiation, Nucleotide metabolism, Sporulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00591; UER00663.

Names & Taxonomyi

Protein namesi
Recommended name:
AMP deaminase (EC:3.5.4.6)
Gene namesi
Name:amdA
ORF Names:DDB_G0292266
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292266. amdA.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells grow normally until development occurs. Then they make a significantly increased proportion of prestalk cells, and develop fruiting bodies with short thick stalks and glassy sori with less than 5% normal spores. They have an increased content of pstA-type prestalk cells. Intracellular levels of inosine increase dramatically during development. This is cell autonomous, as the presence of equal numbers of wild-type cells does not alter the cell type proportion nor improve sporulation. Overexpression of the gene has no apparent effect on development (PubMed:11784104). Mutants lacking amdA form aspidocytes (a cell type able to resist detergent lysis and which are also resistant to some antibiotics) more readily than wild-type cells; their induction may involve AMP or other purine metabolites (PubMed:17259634).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 790790AMP deaminasePRO_0000327654Add
BLAST

Proteomic databases

PRIDEiQ54DD0.

Expressioni

Developmental stagei

Expressed during normal growth and slightly more for the first 4 hours of development. Enzymatic activity increases for the first 5 hours of development and then tapers off.2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB_0191089.

Structurei

3D structure databases

ProteinModelPortaliQ54DD0.
SMRiQ54DD0. Positions 48-656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni292 – 2976Substrate bindingBy similarity
Regioni566 – 5694Substrate bindingBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi745 – 78541Asn-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1816.
InParanoidiQ54DD0.
KOiK01490.
OMAiYENDSAY.
PhylomeDBiQ54DD0.

Family and domain databases

InterProiIPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR006329. AMPD.
[Graphical view]
PANTHERiPTHR11359. PTHR11359. 1 hit.
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01429. AMP_deaminase. 1 hit.
PROSITEiPS00485. A_DEAMINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54DD0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTPLRGSSP QVSFYESELD QEGGSDASHF TYRNYMEDDK INSFTFNMAR
60 70 80 90 100
KDQTQLFQRI ILTNESESEI EEYAEVAEQL LDAINLREKY VFHPKIWKAD
110 120 130 140 150
APVGEKPPYS PFESDESTNC ATEHMFKEVN GVYFVYSNET DMKSNKALFS
160 170 180 190 200
VPHTLASYYK DINNLMMLSS YGPAKTFTFK RLQLLESKFN MHTLLNDSLE
210 220 230 240 250
LFQQKTAPHR DFYNVRKVDT HVHHSSSMNQ KHLLKFIKRK LKENPNEIVI
260 270 280 290 300
FRDDKYLTLA EVFKSLNLDV DELSVDTLDV HADNNTFHRF DKFNLKYNPC
310 320 330 340 350
GQSRLREIFL KTDNLIKGKY LAEISKEVFT DLESSKYQCA EYRLSIYGRK
360 370 380 390 400
MSEWDTLASW IVDNDLFSTK VRWLIQVPRL YDVYRETSTT TFQDFLNNVF
410 420 430 440 450
HPLFEVTKDP SSHPKLHLFL QQVVGIDCVD DESKFEKKFT EKFPVPGEWS
460 470 480 490 500
SEHNPPYTYY LYYLYANLYT LNQFREEKGL NILTLRPHSG EAGEVDHMGA
510 520 530 540 550
AFYLAHGINH GINLRKTPVL QYLYYLTQIG IAMSPLSNNS LFLTYNRNPF
560 570 580 590 600
PAFFARGLNV SISTDDPLQF HYTKEPLMEE YSIATQVWRL SVCDICEIAR
610 620 630 640 650
NSVLQSGFEH NVKSHWLGPD YANSGGNDIK KTNISDIRVC FRNETLIEEL
660 670 680 690 700
HLILKSLQTL PNFKNLNINF LLDKLPSEIT TGNDYKLKKA QLKLNGANKL
710 720 730 740 750
RNSSVGSTPN NGTPSSSGTP SLSSPGAIVH LMKTKPYIPP PLSLNIKQEN
760 770 780 790
NNNNNNNNNN NNNNNNNNTN TNTNSNSTTT NQDDNSKSDK
Length:790
Mass (Da):90,954
Last modified:May 24, 2005 - v1
Checksum:i51ECE82FAA5C6C18
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000189 Genomic DNA. Translation: EAL61257.1.
AF238311 Genomic DNA. Translation: AAF65407.1.
RefSeqiXP_629711.1. XM_629709.1.

Genome annotation databases

EnsemblProtistsiDDB0191089; DDB0191089; DDB_G0292266.
GeneIDi8628625.
KEGGiddi:DDB_G0292266.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000189 Genomic DNA. Translation: EAL61257.1 .
AF238311 Genomic DNA. Translation: AAF65407.1 .
RefSeqi XP_629711.1. XM_629709.1.

3D structure databases

ProteinModelPortali Q54DD0.
SMRi Q54DD0. Positions 48-656.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0191089.

Proteomic databases

PRIDEi Q54DD0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0191089 ; DDB0191089 ; DDB_G0292266 .
GeneIDi 8628625.
KEGGi ddi:DDB_G0292266.

Organism-specific databases

dictyBasei DDB_G0292266. amdA.

Phylogenomic databases

eggNOGi COG1816.
InParanoidi Q54DD0.
KOi K01490.
OMAi YENDSAY.
PhylomeDBi Q54DD0.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00663 .

Family and domain databases

InterProi IPR006650. A/AMP_deam_AS.
IPR001365. A/AMP_deaminase_dom.
IPR006329. AMPD.
[Graphical view ]
PANTHERi PTHR11359. PTHR11359. 1 hit.
Pfami PF00962. A_deaminase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01429. AMP_deaminase. 1 hit.
PROSITEi PS00485. A_DEAMINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Altered cell-type proportioning in Dictyostelium lacking adenosine monophosphate deaminase."
    Chae S.-C., Fuller D., Loomis W.F.
    Dev. Biol. 241:183-194(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-790, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.
    Strain: AX4.
  3. "Purification and characterization of developmentally regulated AMP deaminase from Dictyostelium discoideum."
    Malliaros D.P., Kozwich D.L., Jahngen E.G.E.
    Differentiation 46:153-160(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: AX3.
  4. "A new environmentally resistant cell type from Dictyostelium."
    Serafimidis I., Bloomfield G., Skelton J., Ivens A., Kay R.R.
    Microbiology 153:619-630(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN ASPIDOCYTE FORMATION.
    Strain: AX2.

Entry informationi

Entry nameiAMPD_DICDI
AccessioniPrimary (citable) accession number: Q54DD0
Secondary accession number(s): Q9NGX0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3