Reviewed, UniProtKB/Swiss-Prot Q54DA9 (HACL1_DICDI)

Names and origin

Protein names

Recommended name:
    Probable 2-hydroxyacyl-CoA lyase 1
    EC=4.1.-.-
Alternative name(s):
    2-hydroxyphytanoyl-CoA lyase
      Short name=2-HPCL

Gene names

Name:	hacl1
ORF Names:	DDB_G0292402

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde By similarity.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	carboxy-lyase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	Probable 2-hydroxyacyl-CoA lyase 1		PRO_0000327866
Regions
	Region	413 – 494	82	Thiamine pyrophosphate binding By similarity
Sites
	Metal binding	463	1	Magnesium By similarity
	Metal binding	490	1	Magnesium By similarity
	Binding site	47	1	Thiamine pyrophosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54DA9-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 1C7F03B455883806	FASTA	580	63,165
	10 20 30 40 50 60 MDGVEIIAKS IKNSAIEKVF GIVGVPITPI AYELQAQGVG FFGFRNEQSC SYAASIVGYL 70 80 90 100 110 120 TGLPGLCMTV SGPGVVHALA GVLNAQSNGW PMILLSSSID QSLVGKGGFQ ECKQFESAEL 130 140 150 160 170 180 YCKKCYYLTE IDHFPEILKD AIETSLSNRP GPVYIQIPAD LIKSKCKESP NIREAAGYGT 190 200 210 220 230 240 IAIKSVVPDM KLIKDAVQLL SEAKRPLVIG GKGAAYCRSE NELLEFIEAT KIPFLPSPMG 250 260 270 280 290 300 KGLLRDDHPL VVGAARSYAL KNADVVLVLG ARLNWMFNFG KAPTFSTDVK FIIVDVDENQ 310 320 330 340 350 360 ASKTKNPNVV PEIAIVGDAR LSIAEMRKLF IGETDEQPQP PQDSLIKSMK MEESWWSNLN 370 380 390 400 410 420 QDIQVKTKSL ATLMSEPQNN DQEYLTYHKV FNALRVGGLF QEDTIFVNEG ANTMDIGRLC 430 440 450 460 470 480 IPQTLPRSRL DAGTLATMGV GVGYSVAAQI CFPDRSVVCI QGDSAFGFSA MEMEVAVRYK 490 500 510 520 530 540 LPIVFIVLNN NGVYEGLESM SDPKYTSSTE SASLHIPPTS LSVDTKYELI MQSFGGTGYS 550 560 570 580 ISTISNLLDI CKQIKSKQIS LPTLLNIKIK PTGTKPKIVH

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000190 Genomic DNA. Translation: EAL61180.1.
RefSeq	XP_629587.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3386261.
KEGG	ddi:DDBDRAFT_0184361.
Organism-specific databases
dictyBase	DDB_G0292402. hacl1.
Phylogenomic databases
OMA	Q54DA9. MDIGRTM.
Family and domain databases
InterPro	IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HACL1_DICDI

Accession

Primary (citable) accession number: Q54DA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)