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Protein

Protein LST8 homolog

Gene

lst8

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in regulation of adenylate cyclase and protein kinase B (PKB) activation during aggregation. Involved in both chemotaxis and signal relay.1 Publication

GO - Biological processi

  • activation of adenylate cyclase activity Source: dictyBase
  • activation of protein kinase B activity Source: dictyBase
  • aggregation involved in sorocarp development Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • negative regulation of phagocytosis Source: dictyBase
  • positive regulation of actin filament polymerization Source: dictyBase
  • regulation of myosin II filament assembly Source: dictyBase
  • TOR signaling Source: GO_Central

Keywordsi

Biological processChemotaxis

Enzyme and pathway databases

ReactomeiR-DDI-3371571. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein LST8 homolog
Alternative name(s):
Lethal with sec thirteen 8 protein
Gene namesi
Name:lst8
ORF Names:DDB_G0292592
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292592. lst8.

Subcellular locationi

GO - Cellular componenti

  • autophagosome Source: dictyBase
  • cytoplasm Source: UniProtKB
  • TORC1 complex Source: GO_Central
  • TORC2 complex Source: dictyBase

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells have serious developmental defects, because they are unable to activate the aggregation-stage adenylyl cyclase acaA in response to chemoattractant and are defective in chemotaxis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003265071 – 304Protein LST8 homologAdd BLAST304

Proteomic databases

PaxDbiQ54D08.

Interactioni

Subunit structurei

Part of a complex, TORC1, consisting of tor, raptor and lst8. Part of a complex, TORC2, consisting of tor, lst8, piaA and ripA. Additional proteins, such as 14-3-3 and heat-shock proteins, may also belong to the TORC2 complex.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB0233210.

Structurei

3D structure databases

ProteinModelPortaliQ54D08.
SMRiQ54D08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati1 – 28WD 1Add BLAST28
Repeati31 – 69WD 2Add BLAST39
Repeati74 – 113WD 3Add BLAST40
Repeati115 – 154WD 4Add BLAST40
Repeati158 – 197WD 5Add BLAST40
Repeati205 – 244WD 6Add BLAST40
Repeati247 – 286WD 7Add BLAST40

Sequence similaritiesi

Belongs to the WD repeat LST8 family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0315. Eukaryota.
ENOG410XPVD. LUCA.
InParanoidiQ54D08.
KOiK08266.
OMAiLISCDQA.
PhylomeDBiQ54D08.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiView protein in InterPro
IPR011042. 6-blade_b-propeller_TolB-like.
IPR020472. G-protein_beta_WD-40_rep.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
IPR036322. WD40_repeat_dom_sf.
PfamiView protein in Pfam
PF00400. WD40. 5 hits.
PRINTSiPR00320. GPROTEINBRPT.
SMARTiView protein in SMART
SM00320. WD40. 5 hits.
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiView protein in PROSITE
PS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.

Sequencei

Sequence statusi: Complete.

Q54D08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGIILATAS YDHTIKFWDP PSGGCYRSID CGEFHINRLE ITHDKLYIAA
60 70 80 90 100
AGNPQTRLFE VNTNNNSPAM SFDGHKGNVT GVGFQKEGKW MYTGSEDGTV
110 120 130 140 150
KIWDLKAPGC QRDYECSAPV NTVVLHPNQA ELISGDQNGS IRVWDLISNT
160 170 180 190 200
CSRELVPDGE VGITSLTISS DGGLVVASNT KGKCFVWRLG EDDTSRFEPL
210 220 230 240 250
QKIEAHNAPI LKTLFSPDTK LLATCSADHT VKIWNTKKFN VVQTLNGHQR
260 270 280 290 300
WVWDCAFSND SAYLVTGSSD HLSRLWDLHQ GDAVKTYSGH IKAVNAVALN

DLPR
Length:304
Mass (Da):33,481
Last modified:May 24, 2005 - v1
Checksum:iEE6F2E5DBCCA7070
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000194 Genomic DNA. Translation: EAL61099.1.
RefSeqiXP_629512.1. XM_629510.1.

Genome annotation databases

EnsemblProtistsiEAL61099; EAL61099; DDB_G0292592.
GeneIDi8628763.
KEGGiddi:DDB_G0292592.

Similar proteinsi

Entry informationi

Entry nameiLST8_DICDI
AccessioniPrimary (citable) accession number: Q54D08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: October 25, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families