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Protein

Protein LST8 homolog

Gene

lst8

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in regulation of adenylate cyclase and protein kinase B (PKB) activation during aggregation. Involved in both chemotaxis and signal relay.1 Publication

GO - Biological processi

  • activation of adenylate cyclase activity Source: dictyBase
  • activation of protein kinase B activity Source: dictyBase
  • aggregation involved in sorocarp development Source: dictyBase
  • chemotaxis to cAMP Source: dictyBase
  • negative regulation of phagocytosis Source: dictyBase
  • positive regulation of actin filament polymerization Source: dictyBase
  • regulation of myosin II filament assembly Source: dictyBase
Complete GO annotation...

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

ReactomeiR-DDI-3371571. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein LST8 homolog
Alternative name(s):
Lethal with sec thirteen 8 protein
Gene namesi
Name:lst8
ORF Names:DDB_G0292592
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292592. lst8.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • TORC2 complex Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Cells have serious developmental defects, because they are unable to activate the aggregation-stage adenylyl cyclase acaA in response to chemoattractant and are defective in chemotaxis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Protein LST8 homologPRO_0000326507Add
BLAST

Proteomic databases

PaxDbiQ54D08.
PRIDEiQ54D08.

Interactioni

Subunit structurei

Part of a complex, TORC1, consisting of tor, raptor and lst8. Part of a complex, TORC2, consisting of tor, lst8, piaA and ripA. Additional proteins, such as 14-3-3 and heat-shock proteins, may also belong to the TORC2 complex.1 Publication

Protein-protein interaction databases

STRINGi44689.DDB0233210.

Structurei

3D structure databases

ProteinModelPortaliQ54D08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 2828WD 1Add
BLAST
Repeati31 – 6939WD 2Add
BLAST
Repeati74 – 11340WD 3Add
BLAST
Repeati115 – 15440WD 4Add
BLAST
Repeati158 – 19740WD 5Add
BLAST
Repeati205 – 24440WD 6Add
BLAST
Repeati247 – 28640WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat LST8 family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0315. Eukaryota.
ENOG410XPVD. LUCA.
InParanoidiQ54D08.
KOiK08266.
OMAiRIWDLGE.
PhylomeDBiQ54D08.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q54D08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGIILATAS YDHTIKFWDP PSGGCYRSID CGEFHINRLE ITHDKLYIAA
60 70 80 90 100
AGNPQTRLFE VNTNNNSPAM SFDGHKGNVT GVGFQKEGKW MYTGSEDGTV
110 120 130 140 150
KIWDLKAPGC QRDYECSAPV NTVVLHPNQA ELISGDQNGS IRVWDLISNT
160 170 180 190 200
CSRELVPDGE VGITSLTISS DGGLVVASNT KGKCFVWRLG EDDTSRFEPL
210 220 230 240 250
QKIEAHNAPI LKTLFSPDTK LLATCSADHT VKIWNTKKFN VVQTLNGHQR
260 270 280 290 300
WVWDCAFSND SAYLVTGSSD HLSRLWDLHQ GDAVKTYSGH IKAVNAVALN

DLPR
Length:304
Mass (Da):33,481
Last modified:May 24, 2005 - v1
Checksum:iEE6F2E5DBCCA7070
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000194 Genomic DNA. Translation: EAL61099.1.
RefSeqiXP_629512.1. XM_629510.1.

Genome annotation databases

EnsemblProtistsiDDB0233210; DDB0233210; DDB_G0292592.
GeneIDi8628763.
KEGGiddi:DDB_G0292592.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000194 Genomic DNA. Translation: EAL61099.1.
RefSeqiXP_629512.1. XM_629510.1.

3D structure databases

ProteinModelPortaliQ54D08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB0233210.

Proteomic databases

PaxDbiQ54D08.
PRIDEiQ54D08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0233210; DDB0233210; DDB_G0292592.
GeneIDi8628763.
KEGGiddi:DDB_G0292592.

Organism-specific databases

dictyBaseiDDB_G0292592. lst8.

Phylogenomic databases

eggNOGiKOG0315. Eukaryota.
ENOG410XPVD. LUCA.
InParanoidiQ54D08.
KOiK08266.
OMAiRIWDLGE.
PhylomeDBiQ54D08.

Enzyme and pathway databases

ReactomeiR-DDI-3371571. HSF1-dependent transactivation.

Miscellaneous databases

PROiQ54D08.

Family and domain databases

Gene3Di2.130.10.10. 2 hits.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF00400. WD40. 4 hits.
[Graphical view]
PRINTSiPR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 5 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 4 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLST8_DICDI
AccessioniPrimary (citable) accession number: Q54D08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 7, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.