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Protein

Probable malate dehydrogenase 2, mitochondrial

Gene

mdhB

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei112SubstrateBy similarity1
Binding sitei118SubstrateBy similarity1
Binding sitei125NADBy similarity1
Binding sitei132NADBy similarity1
Binding sitei152SubstrateBy similarity1
Binding sitei183SubstrateBy similarity1
Active sitei208Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 37NADBy similarity7
Nucleotide bindingi150 – 152NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Probable malate dehydrogenase 2, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:mdhB
ORF Names:DDB_G0292600
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292600. mdhB.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • mitochondrion Source: UniProtKB-SubCell
  • phagocytic vesicle Source: dictyBase

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 9MitochondrionSequence analysis9
ChainiPRO_000031234810 – 348Probable malate dehydrogenase 2, mitochondrialAdd BLAST339

Proteomic databases

PaxDbiQ54D04.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi44689.DDB0230188.

Structurei

3D structure databases

ProteinModelPortaliQ54D04.
SMRiQ54D04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
InParanoidiQ54D04.
KOiK00024.
OMAiAGQISNH.
PhylomeDBiQ54D04.

Family and domain databases

HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54D04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKILTRSFS TGANFFASGK GKAPVRVAIT GASGQIGYQL LFRIASGDML
60 70 80 90 100
GKDQPIILQC LELPGAMNSL KGVSMELDDC AFPLLKGIVQ SDKPEEAFAG
110 120 130 140 150
ADYALLVGAR PRSKGMERGD LLKANAEIFS VQGKALDKSA NRDTLRVLVV
160 170 180 190 200
GNPANTNALI AARNAPNIDP KRFSAMTRLD HNRGLAQLAD KTGSAVTDIE
210 220 230 240 250
KFCIWGNHSA TQYPDINFGT VKGKSLVDTI NDQKWVKDNF IPTVQQRGAA
260 270 280 290 300
IIAARGLSSA ASAASAAIDH MRDWTYGTNG QWTSMAIYSE GEYGADKGLY
310 320 330 340
FSFPVIVDNK GKYEIVKGLK LDQFSQERFD ATRKELLSEM DGVKELLP
Length:348
Mass (Da):37,648
Last modified:May 24, 2005 - v1
Checksum:iA70092840B76C58B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000194 Genomic DNA. Translation: EAL61103.1.
RefSeqiXP_629516.1. XM_629514.1.

Genome annotation databases

EnsemblProtistsiEAL61103; EAL61103; DDB_G0292600.
GeneIDi8628767.
KEGGiddi:DDB_G0292600.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMDHB_DICDI
AccessioniPrimary (citable) accession number: Q54D04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 24, 2005
Last modified: June 7, 2017
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families