Reviewed, UniProtKB/Swiss-Prot Q54D04 (MDHB_DICDI)

Names and origin

Protein names

Recommended name:
    Probable malate dehydrogenase 2, mitochondrial
    EC=1.1.1.37

Gene names

Name:	mdhB
ORF Names:	DDB_G0292600

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion Potential.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 2 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 9	9	Mitochondrion Potential
	Chain	10 – 348	339	Probable malate dehydrogenase 2, mitochondrial		PRO_0000312348
Regions
	Nucleotide binding	31 – 37	7	NAD By similarity
	Nucleotide binding	150 – 152	3	NAD By similarity
Sites
	Active site	208	1	Proton acceptor By similarity
	Binding site	112	1	Substrate By similarity
	Binding site	118	1	Substrate By similarity
	Binding site	125	1	NAD By similarity
	Binding site	132	1	NAD By similarity
	Binding site	152	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54D04-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: A70092840B76C58B	FASTA	348	37,648
	10 20 30 40 50 60 MNKILTRSFS TGANFFASGK GKAPVRVAIT GASGQIGYQL LFRIASGDML GKDQPIILQC 70 80 90 100 110 120 LELPGAMNSL KGVSMELDDC AFPLLKGIVQ SDKPEEAFAG ADYALLVGAR PRSKGMERGD 130 140 150 160 170 180 LLKANAEIFS VQGKALDKSA NRDTLRVLVV GNPANTNALI AARNAPNIDP KRFSAMTRLD 190 200 210 220 230 240 HNRGLAQLAD KTGSAVTDIE KFCIWGNHSA TQYPDINFGT VKGKSLVDTI NDQKWVKDNF 250 260 270 280 290 300 IPTVQQRGAA IIAARGLSSA ASAASAAIDH MRDWTYGTNG QWTSMAIYSE GEYGADKGLY 310 320 330 340 FSFPVIVDNK GKYEIVKGLK LDQFSQERFD ATRKELLSEM DGVKELLP

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000194 Genomic DNA. Translation: EAL61103.1.
RefSeq	XP_629516.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54D04.
Genome annotation databases
GeneID	3386244.
GenomeReviews	Gene locus mdhB in contig CM000155_GR.
KEGG	ddi:DDB_0230188.
Organism-specific databases
dictyBase	DDB_G0292600. mdhB.
Phylogenomic databases
OMA	CLYDPYA.
Enzyme and pathway databases
BRENDA	1.1.1.37. 424.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR010945. Malate_DH_SF1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23382. MDH_SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
ProDom	PD003052. Mal_dehydrog. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01759. MalateDH-SF1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDHB_DICDI

Accession

Primary (citable) accession number: Q54D04

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)