SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q54D04

- MDHB_DICDI

UniProt

Q54D04 - MDHB_DICDI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Probable malate dehydrogenase 2, mitochondrial
Gene
mdhB, DDB_G0292600
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei112 – 1121Substrate By similarity
Binding sitei118 – 1181Substrate By similarity
Binding sitei125 – 1251NAD By similarity
Binding sitei132 – 1321NAD By similarity
Binding sitei152 – 1521Substrate By similarity
Binding sitei183 – 1831Substrate By similarity
Active sitei208 – 2081Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 377NAD By similarity
Nucleotide bindingi150 – 1523NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Probable malate dehydrogenase 2, mitochondrial (EC:1.1.1.37)
Gene namesi
Name:mdhB
ORF Names:DDB_G0292600
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0292600. mdhB.

Subcellular locationi

Mitochondrion Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
  2. phagocytic vesicle Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 99Mitochondrion Reviewed prediction
Chaini10 – 348339Probable malate dehydrogenase 2, mitochondrialUniRule annotation
PRO_0000312348Add
BLAST

Proteomic databases

PRIDEiQ54D04.

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi44689.DDB_0230188.

Structurei

3D structure databases

ProteinModelPortaliQ54D04.
SMRiQ54D04. Positions 22-346.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
OMAiCALRDEV.
PhylomeDBiQ54D04.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54D04-1 [UniParc]FASTAAdd to Basket

« Hide

MNKILTRSFS TGANFFASGK GKAPVRVAIT GASGQIGYQL LFRIASGDML    50
GKDQPIILQC LELPGAMNSL KGVSMELDDC AFPLLKGIVQ SDKPEEAFAG 100
ADYALLVGAR PRSKGMERGD LLKANAEIFS VQGKALDKSA NRDTLRVLVV 150
GNPANTNALI AARNAPNIDP KRFSAMTRLD HNRGLAQLAD KTGSAVTDIE 200
KFCIWGNHSA TQYPDINFGT VKGKSLVDTI NDQKWVKDNF IPTVQQRGAA 250
IIAARGLSSA ASAASAAIDH MRDWTYGTNG QWTSMAIYSE GEYGADKGLY 300
FSFPVIVDNK GKYEIVKGLK LDQFSQERFD ATRKELLSEM DGVKELLP 348
Length:348
Mass (Da):37,648
Last modified:May 24, 2005 - v1
Checksum:iA70092840B76C58B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000194 Genomic DNA. Translation: EAL61103.1.
RefSeqiXP_629516.1. XM_629514.1.

Genome annotation databases

EnsemblProtistsiDDB0230188; DDB0230188; DDB_G0292600.
GeneIDi8628767.
KEGGiddi:DDB_G0292600.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000194 Genomic DNA. Translation: EAL61103.1 .
RefSeqi XP_629516.1. XM_629514.1.

3D structure databases

ProteinModelPortali Q54D04.
SMRi Q54D04. Positions 22-346.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0230188.

Proteomic databases

PRIDEi Q54D04.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0230188 ; DDB0230188 ; DDB_G0292600 .
GeneIDi 8628767.
KEGGi ddi:DDB_G0292600.

Organism-specific databases

dictyBasei DDB_G0292600. mdhB.

Phylogenomic databases

eggNOGi COG0039.
OMAi CALRDEV.
PhylomeDBi Q54D04.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPi MF_01517. Malate_dehydrog_2.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. Bienvenut W.V., Veltman D.M., Insall R.H.
    Submitted (JAN-2010) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 124-134 AND 147-163, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiMDHB_DICDI
AccessioniPrimary (citable) accession number: Q54D04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: May 24, 2005
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi