Reviewed, UniProtKB/Swiss-Prot Q54CX6 (3HIDH_DICDI)

Names and origin

Protein names

Recommended name:
    Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial
      Short name=HIBADH
    EC=1.1.1.31

Gene names

Name:	hibA
ORF Names:	DDB_G0292566

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the 3-hydroxyisobutyrate dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion By similarity
	Chain	? – 321		Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial		PRO_0000327881
Regions
	Nucleotide binding	23 – 52	30	NAD By similarity
	Nucleotide binding	86 – 87	2	NAD By similarity
Sites
	Active site	192	1	By similarity
	Binding site	117	1	NAD; via amide nitrogen By similarity
	Binding site	267	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54CX6-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 10F0626EFAB0AB7A	FASTA	321	33,936
	10 20 30 40 50 60 MFSSKKSLLL FKNVRYMSTS SSKTVGFIGL GNMGGHQAIN LIKKGHNLIV FDMSKDNMNR 70 80 90 100 110 120 LKEKGAKIAN SPAEVAKEAD VIVTMLPASA HVKNVYCGEN GIFQTVRPGT LLLDSSTIDP 130 140 150 160 170 180 ATAREVASIA KKHQSTMLDC PVSGGTGGAE AGTLTFMVGG SEQDFNTAKT YLECMGKNIV 190 200 210 220 230 240 HCGDVGTGQV AKVCNNLVLG ISMIAVSEAM NLGVKQGMDP KKLAGIFNTS SARCWTSELY 250 260 270 280 290 300 NPCPGVIETS PASRGYTGGF GSALMTKDLG LAVDSAKSIG EPLLLGNSAH QLYTLLVAKG 310 320 DGQKDFSVVY DFLNKNFKNS N

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000194 Genomic DNA. Translation: EAL61086.1.
RefSeq	XP_629544.1.
3D structure databases
HSSP	HSSP built from PDB template 2GF2 based on UniProtKB P31937.
SMR	Q54CX6. Positions 23-315.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54CX6.
Genome annotation databases
GeneID	8628802.
KEGG	ddi:DDB_0216217.
Organism-specific databases
dictyBase	DDB_G0292566. hibA.
Phylogenomic databases
HOGENOM	HBG729179.
OMA	PMARNLI.
PhylomeDB	Q54CX6.
Enzyme and pathway databases
BRENDA	1.1.1.31. 424.
Family and domain databases
InterPro	IPR015815. 3hydroxyacid_DH/Rdtase. IPR008927. 6-PGluconate_DH_C-like. IPR006115. 6PGDH_NAD-bd. IPR013328. DH_multihelical. IPR011548. IsoBut3OH_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR22981. 3hydroxy_acid_DH. 1 hit.
Pfam	PF03446. NAD_binding_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01692. HIBADH. 1 hit.
PROSITE	PS00895. 3_HYDROXYISOBUT_DH. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

3HIDH_DICDI

Accession

Primary (citable) accession number: Q54CX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)