Reviewed, UniProtKB/Swiss-Prot Q54CN7 (CYSK_DICDI)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	cysK
ORF Names:	DDB_G0292840

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	Cysteine synthase		PRO_0000328233
Regions
	Region	215 – 219	5	Pyridoxal phosphate binding By similarity
	Compositional bias	20 – 28	9	Poly-Asn
Sites
	Binding site	319	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	79	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54CN7-1 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 56CDA9DF8ABD272D	FASTA	378	41,101
	10 20 30 40 50 60 MFSSIYGYFN SEGDSNQQQN NNNNSNNNLK ESVFHSGISN GIIETVGNTP LIRIKSLSEA 70 80 90 100 110 120 TGCEIYGKAE FMNPGGSPKD RVAREIILDG EKKGLLKKGS TIVEATAGST GISLTMLGKS 130 140 150 160 170 180 RGYNVQLFIP DNVSKEKVDL LEMLGAETKI VPIVGMNNAN HFMHCAYQRC LGDDMAFYAN 190 200 210 220 230 240 QFDNLSNFNA HYNGTAKEIW EQTKGDVDGF VAAAGTGGTV AGISSYLKEV NPNIQNWLID 250 260 270 280 290 300 PPGSGLYSLV NTGVIFHPKD RLVVEKLGPR SFYEGVGVNK KTENFNKASL NGAFRGTEEE 310 320 330 340 350 360 GVDMAHYLLK HDGLFLGGSS ALNCVGAVKL ARKLGPGKTI VTVLCDSGHR YTSRLYSKSW 370 LNDHNFQVSD LSNLNFVK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000197 Genomic DNA. Translation: EAL60969.1.
RefSeq	XP_629379.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q54CN7.
Genome annotation databases
GeneID	3386032.
GenomeReviews	Gene locus cysK in contig CM000155_GR.
KEGG	ddi:DDB_0230066.
Organism-specific databases
dictyBase	DDB_G0292840. cysK.
Phylogenomic databases
OMA	RVRPVSI.
Enzyme and pathway databases
BRENDA	2.5.1.47. 424.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK_DICDI

Accession

Primary (citable) accession number: Q54CN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 3, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)