Q54CM0 (PPT3_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyl-protein thioesterase 3
Short name=PPT-3
EC=3.1.2.22

Alternative name(s):
Palmitoyl-protein hydrolase 3

Gene names

Name:	ppt3
ORF Names:	DDB_G0292862

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation By similarity.
Catalytic activity	Palmitoyl-protein + H2O = palmitate + protein.
Subcellular location	Lysosome By similarity.
Sequence similarities	Belongs to the palmitoyl-protein thioesterase family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	palmitoyl-(protein) hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 289	269	Palmitoyl-protein thioesterase 3		PRO_0000328588
Sites
	Active site	97	1	By similarity
	Active site	210	1	By similarity
	Active site	266	1	By similarity
Amino acid modifications
	Glycosylation	189	1	N-linked (GlcNAc...) Potential
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	281	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q54CM0 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: 4CD378FD612CEF66	FASTA	289	33,050
	10 20 30 40 50 60 MRILSSLILL IALAIALVSA TRPVVLMHGV TTGKESMEPL KSWIEESIPD IYVLNVEIGN 70 80 90 100 110 120 GAFDSIFTTM DSQIEEFAQV VQADPKLANG FNLIGFSQGT LIARAFVQRY NNPQVYNYIS 130 140 150 160 170 180 WNGPQGGQFG TPFVNIPWVD KVLGTIPYEK TIQKKLSVAE YWKDPHRIDK YLERSIFLAD 190 200 210 220 230 240 INNEYQVKNT TYKENLTKLN AMVLTYSTND KTIIPKESGW FSFYADGSGT EVVPLQQQTQ 250 260 270 280 YSEDWLGLRT LDESNRLFFY TTTCTHRDHP IEDYCKPYFT NFTLPYLQN

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000197 Genomic DNA. Translation: EAL60980.1.
RefSeq	XP_629396.1. XM_629394.1.
3D structure databases
HSSP	HSSP built from PDB template 1EI9 based on UniProtKB P45478.
ProteinModelPortal	Q54CM0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0233893; DDB0233893; DDB_G0292862.
GeneID	8628914.
GenomeReviews	Gene locus ppt3 in contig CM000155_GR.
KEGG	ddi:DDB_G0292862.
Organism-specific databases
dictyBase	DDB_G0292862. ppt3.
Phylogenomic databases
eggNOG	KOG2541.
GeneTree	EPrGT00050000005467.
HOGENOM	HBG444329.
OMA	AEIACEN.
PhylomeDB	Q54CM0.
ProtClustDB	CLSZ2429345.
Family and domain databases
InterPro	IPR002472. Palm_thioest. [Graphical view]
KO	K01074.
Pfam	PF02089. Palm_thioest. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPT3_DICDI

Accession

Primary (citable) accession number: Q54CM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	November 16, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)