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Protein

Probable isoleucine--tRNA ligase, mitochondrial

Gene

mileS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei658ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:mileS
ORF Names:DDB_G0293030
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyostelidsDictyostelialesDictyosteliaceaeDictyostelium
Proteomesi
  • UP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0293030 mileS

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionSequence analysisAdd BLAST32
ChainiPRO_000032797033 – 1034Probable isoleucine--tRNA ligase, mitochondrialAdd BLAST1002

Proteomic databases

PaxDbiQ54CE4

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB0231256

Structurei

3D structure databases

ProteinModelPortaliQ54CE4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi94 – 104"HIGH" regionBy similarityAdd BLAST11
Motifi655 – 659"KMSKS" regionBy similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi956 – 971Poly-GlnAdd BLAST16

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0433 Eukaryota
COG0060 LUCA
InParanoidiQ54CE4
KOiK01870
OMAiHLGTAWN
PhylomeDBiQ54CE4

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 2 hits
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54CE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISLNNSFFN KRVIVNSFNN YKRSFGTKSQ NEESAIVDSN HSFAHTLNLP
60 70 80 90 100
KTTFSMKANA ATREPTLLKD PYKLYKWQLE NNKGENWVFH DGPPYANGDL
110 120 130 140 150
HMGHALNKIL KDIVNRYKVL KGFKVNYIPG WDCHGLPIEQ QAFKKLKKSS
160 170 180 190 200
DMKASDIRKI AGDFARKEIE KQSKGFQEMG ILGDWENPYK TLDYGYEVEQ
210 220 230 240 250
IQTFYDMFNK GYIYRGVKPV HWSPSSRTAL ADAELEYNNN HTSKSIFVKF
260 270 280 290 300
NVKSLSNHIL NNLPTTSDIN KAEMVISAII WTTTPWTIPA NQAICVNSEM
310 320 330 340 350
DYILVKPIES EQYRNEMFII SKERLESLTK SFNIGELKVI LEFKGEQLKG
360 370 380 390 400
TITKHPQYDR ESPIITGDHV IEGSGTGLVH TAPGHGVEDF QICQQQYPDL
410 420 430 440 450
KVLSPVNDLG CFTDEVGEKF VGLEVLGDGN EAVINDLETI GSLLHKEDYI
460 470 480 490 500
HKYPYDWRTK KPIIIRTTLQ WFVGLKNIQK TALQSIERVN MVPPSGSNRL
510 520 530 540 550
SSMIGKRTDW CISRQRVWGC PIPVLYNCKT NEPLINDESI NHIKELFGKF
560 570 580 590 600
GSDCWFEMST QQLLPPSLKD QHENFVKGTD TMDVWFDSGT SWRGVLVERG
610 620 630 640 650
IIDKDTGRAD IYLEGSDQHR GWFQSSLLTS VCVRDIEPYK NVVTHGFLLD
660 670 680 690 700
ESGIKMSKSI GNTIVPSTVI KGGPNKVQNP PYGVDLLRTW VASSDYSKDI
710 720 730 740 750
SIGPNILIKI LDGIKKIRNT LRFMLASNFD FDPTIHAIPY EKLSSLDKYA
760 770 780 790 800
LHRVFKLQES VTRHYDQFQF QKVHTEIINF SIEISSFYFD VIKRHLYAES
810 820 830 840 850
PNSHSRRSTQ TVLFKMLDVI NIALAPITVH TSEDVFLHQY QFKNNGKGLD
860 870 880 890 900
KNLIENSVFA HGWDQLPSQY ENELISNQFT NIIAIRDVVN RVLQSMRSQG
910 920 930 940 950
IIGRSDETIM ELTVTNEESS PFYDNLFAIN SQLDDIFCVS NVLLKKFNTF
960 970 980 990 1000
EKDVEQQQQQ QQQQQQQQPQ QIEPNSKGEF IFNTIISNNS KQLGRIEVLL
1010 1020 1030
KISDKFKCPR CWRHTSIEND KVCKPCDSVL NSLK
Length:1,034
Mass (Da):118,422
Last modified:May 24, 2005 - v1
Checksum:iCD81811C34E14FB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000199 Genomic DNA Translation: EAL60867.1
RefSeqiXP_629275.1, XM_629273.1

Genome annotation databases

EnsemblProtistsiEAL60867; EAL60867; DDB_G0293030
GeneIDi8628997
KEGGiddi:DDB_G0293030

Similar proteinsi

Entry informationi

Entry nameiSYIM_DICDI
AccessioniPrimary (citable) accession number: Q54CE4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: March 28, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health