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Q54CE4 (SYIM_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable isoleucine--tRNA ligase, mitochondrial

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:mileS
ORF Names:DDB_G0293030
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length1034 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Subcellular location

Mitochondrion matrix By similarity HAMAP-Rule MF_02002.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3232Mitochondrion Potential
Chain33 – 10341002Probable isoleucine--tRNA ligase, mitochondrial HAMAP-Rule MF_02002
PRO_0000327970

Regions

Motif94 – 10411"HIGH" region By similarity
Motif655 – 6595"KMSKS" region By similarity
Compositional bias956 – 97116Poly-Gln HAMAP-Rule MF_02002

Sites

Binding site6581ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q54CE4 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: CD81811C34E14FB9

FASTA1,034118,422
        10         20         30         40         50         60 
MISLNNSFFN KRVIVNSFNN YKRSFGTKSQ NEESAIVDSN HSFAHTLNLP KTTFSMKANA 

        70         80         90        100        110        120 
ATREPTLLKD PYKLYKWQLE NNKGENWVFH DGPPYANGDL HMGHALNKIL KDIVNRYKVL 

       130        140        150        160        170        180 
KGFKVNYIPG WDCHGLPIEQ QAFKKLKKSS DMKASDIRKI AGDFARKEIE KQSKGFQEMG 

       190        200        210        220        230        240 
ILGDWENPYK TLDYGYEVEQ IQTFYDMFNK GYIYRGVKPV HWSPSSRTAL ADAELEYNNN 

       250        260        270        280        290        300 
HTSKSIFVKF NVKSLSNHIL NNLPTTSDIN KAEMVISAII WTTTPWTIPA NQAICVNSEM 

       310        320        330        340        350        360 
DYILVKPIES EQYRNEMFII SKERLESLTK SFNIGELKVI LEFKGEQLKG TITKHPQYDR 

       370        380        390        400        410        420 
ESPIITGDHV IEGSGTGLVH TAPGHGVEDF QICQQQYPDL KVLSPVNDLG CFTDEVGEKF 

       430        440        450        460        470        480 
VGLEVLGDGN EAVINDLETI GSLLHKEDYI HKYPYDWRTK KPIIIRTTLQ WFVGLKNIQK 

       490        500        510        520        530        540 
TALQSIERVN MVPPSGSNRL SSMIGKRTDW CISRQRVWGC PIPVLYNCKT NEPLINDESI 

       550        560        570        580        590        600 
NHIKELFGKF GSDCWFEMST QQLLPPSLKD QHENFVKGTD TMDVWFDSGT SWRGVLVERG 

       610        620        630        640        650        660 
IIDKDTGRAD IYLEGSDQHR GWFQSSLLTS VCVRDIEPYK NVVTHGFLLD ESGIKMSKSI 

       670        680        690        700        710        720 
GNTIVPSTVI KGGPNKVQNP PYGVDLLRTW VASSDYSKDI SIGPNILIKI LDGIKKIRNT 

       730        740        750        760        770        780 
LRFMLASNFD FDPTIHAIPY EKLSSLDKYA LHRVFKLQES VTRHYDQFQF QKVHTEIINF 

       790        800        810        820        830        840 
SIEISSFYFD VIKRHLYAES PNSHSRRSTQ TVLFKMLDVI NIALAPITVH TSEDVFLHQY 

       850        860        870        880        890        900 
QFKNNGKGLD KNLIENSVFA HGWDQLPSQY ENELISNQFT NIIAIRDVVN RVLQSMRSQG 

       910        920        930        940        950        960 
IIGRSDETIM ELTVTNEESS PFYDNLFAIN SQLDDIFCVS NVLLKKFNTF EKDVEQQQQQ 

       970        980        990       1000       1010       1020 
QQQQQQQQPQ QIEPNSKGEF IFNTIISNNS KQLGRIEVLL KISDKFKCPR CWRHTSIEND 

      1030 
KVCKPCDSVL NSLK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000199 Genomic DNA. Translation: EAL60867.1.

3D structure databases

ProteinModelPortalQ54CE4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0231256.

Proteomic databases

PRIDEQ54CE4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0231256; DDB0231256; DDB_G0293030.
KEGGddi:DDB_G0293030.

Organism-specific databases

dictyBaseDDB_G0293030. mileS.

Phylogenomic databases

eggNOGCOG0060.
KOK01870.
OMAVLGDWDN.
PhylomeDBQ54CE4.
ProtClustDBCLSZ2429312.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ54CE4.

Entry information

Entry nameSYIM_DICDI
AccessionPrimary (citable) accession number: Q54CE4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries