Q54CE4 (SYIM_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable isoleucine--tRNA ligase, mitochondrial
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	mileS
ORF Names:	DDB_G0293030

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1034 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoleucyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: dictyBase
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW isoleucine-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 32	32	Mitochondrion Potential
	Chain	33 – 1034	1002	Probable isoleucine--tRNA ligase, mitochondrial		PRO_0000327970
Regions
	Motif	94 – 104	11	"HIGH" region By similarity
	Motif	655 – 659	5	"KMSKS" region By similarity
	Compositional bias	956 – 971	16	Poly-Gln
Sites
	Binding site	658	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q54CE4 [UniParc]. Last modified May 24, 2005. Version 1. Checksum: CD81811C34E14FB9	FASTA	1,034	118,422
	10 20 30 40 50 60 MISLNNSFFN KRVIVNSFNN YKRSFGTKSQ NEESAIVDSN HSFAHTLNLP KTTFSMKANA 70 80 90 100 110 120 ATREPTLLKD PYKLYKWQLE NNKGENWVFH DGPPYANGDL HMGHALNKIL KDIVNRYKVL 130 140 150 160 170 180 KGFKVNYIPG WDCHGLPIEQ QAFKKLKKSS DMKASDIRKI AGDFARKEIE KQSKGFQEMG 190 200 210 220 230 240 ILGDWENPYK TLDYGYEVEQ IQTFYDMFNK GYIYRGVKPV HWSPSSRTAL ADAELEYNNN 250 260 270 280 290 300 HTSKSIFVKF NVKSLSNHIL NNLPTTSDIN KAEMVISAII WTTTPWTIPA NQAICVNSEM 310 320 330 340 350 360 DYILVKPIES EQYRNEMFII SKERLESLTK SFNIGELKVI LEFKGEQLKG TITKHPQYDR 370 380 390 400 410 420 ESPIITGDHV IEGSGTGLVH TAPGHGVEDF QICQQQYPDL KVLSPVNDLG CFTDEVGEKF 430 440 450 460 470 480 VGLEVLGDGN EAVINDLETI GSLLHKEDYI HKYPYDWRTK KPIIIRTTLQ WFVGLKNIQK 490 500 510 520 530 540 TALQSIERVN MVPPSGSNRL SSMIGKRTDW CISRQRVWGC PIPVLYNCKT NEPLINDESI 550 560 570 580 590 600 NHIKELFGKF GSDCWFEMST QQLLPPSLKD QHENFVKGTD TMDVWFDSGT SWRGVLVERG 610 620 630 640 650 660 IIDKDTGRAD IYLEGSDQHR GWFQSSLLTS VCVRDIEPYK NVVTHGFLLD ESGIKMSKSI 670 680 690 700 710 720 GNTIVPSTVI KGGPNKVQNP PYGVDLLRTW VASSDYSKDI SIGPNILIKI LDGIKKIRNT 730 740 750 760 770 780 LRFMLASNFD FDPTIHAIPY EKLSSLDKYA LHRVFKLQES VTRHYDQFQF QKVHTEIINF 790 800 810 820 830 840 SIEISSFYFD VIKRHLYAES PNSHSRRSTQ TVLFKMLDVI NIALAPITVH TSEDVFLHQY 850 860 870 880 890 900 QFKNNGKGLD KNLIENSVFA HGWDQLPSQY ENELISNQFT NIIAIRDVVN RVLQSMRSQG 910 920 930 940 950 960 IIGRSDETIM ELTVTNEESS PFYDNLFAIN SQLDDIFCVS NVLLKKFNTF EKDVEQQQQQ 970 980 990 1000 1010 1020 QQQQQQQQPQ QIEPNSKGEF IFNTIISNNS KQLGRIEVLL KISDKFKCPR CWRHTSIEND 1030 KVCKPCDSVL NSLK

References

[1]

"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000199 Genomic DNA. Translation: EAL60867.1.
RefSeq	XP_629275.1. XM_629273.1.
3D structure databases
HSSP	HSSP built from PDB template 1QU3 based on UniProtKB P41972.
ProteinModelPortal	Q54CE4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q54CE4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231256; DDB0231256; DDB_G0293030.
GeneID	8628997.
GenomeReviews	Gene locus mileS in contig CM000155_GR.
KEGG	ddi:DDB_G0293030.
Organism-specific databases
dictyBase	DDB_G0293030. mileS.
Phylogenomic databases
eggNOG	KOG0433.
GeneTree	EPrGT00050000002243.
HOGENOM	HBG577712.
OMA	KQVLTHG.
PhylomeDB	Q54CE4.
ProtClustDB	CLSZ2429312.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-synt. IPR023585. Ile-tRNA-synt_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
Gene3D	G3DSA:3.90.740.10. G3DSA:3.90.740.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01870.
PANTHER	PTHR11946:SF9. Ile-tRNA-synt_Ia. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. IleS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIM_DICDI

Accession

Primary (citable) accession number: Q54CE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)