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Protein

Probable isoleucine--tRNA ligase, mitochondrial

Gene

mileS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei658 – 6581ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. isoleucine-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: GO_Central
  2. mitochondrial translation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:mileS
ORF Names:DDB_G0293030
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195 Componentsi: Chromosome 6, Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0293030. mileS.

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. cytosol Source: GO_Central
  2. mitochondrial matrix Source: UniProtKB-SubCell
  3. mitochondrion Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232MitochondrionSequence AnalysisAdd
BLAST
Chaini33 – 10341002Probable isoleucine--tRNA ligase, mitochondrialPRO_0000327970Add
BLAST

Proteomic databases

PRIDEiQ54CE4.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231256.

Structurei

3D structure databases

ProteinModelPortaliQ54CE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi94 – 10411"HIGH" regionBy similarityAdd
BLAST
Motifi655 – 6595"KMSKS" regionBy similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi956 – 97116Poly-GlnAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0060.
InParanoidiQ54CE4.
KOiK01870.
OMAiQECRKFA.
PhylomeDBiQ54CE4.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54CE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISLNNSFFN KRVIVNSFNN YKRSFGTKSQ NEESAIVDSN HSFAHTLNLP
60 70 80 90 100
KTTFSMKANA ATREPTLLKD PYKLYKWQLE NNKGENWVFH DGPPYANGDL
110 120 130 140 150
HMGHALNKIL KDIVNRYKVL KGFKVNYIPG WDCHGLPIEQ QAFKKLKKSS
160 170 180 190 200
DMKASDIRKI AGDFARKEIE KQSKGFQEMG ILGDWENPYK TLDYGYEVEQ
210 220 230 240 250
IQTFYDMFNK GYIYRGVKPV HWSPSSRTAL ADAELEYNNN HTSKSIFVKF
260 270 280 290 300
NVKSLSNHIL NNLPTTSDIN KAEMVISAII WTTTPWTIPA NQAICVNSEM
310 320 330 340 350
DYILVKPIES EQYRNEMFII SKERLESLTK SFNIGELKVI LEFKGEQLKG
360 370 380 390 400
TITKHPQYDR ESPIITGDHV IEGSGTGLVH TAPGHGVEDF QICQQQYPDL
410 420 430 440 450
KVLSPVNDLG CFTDEVGEKF VGLEVLGDGN EAVINDLETI GSLLHKEDYI
460 470 480 490 500
HKYPYDWRTK KPIIIRTTLQ WFVGLKNIQK TALQSIERVN MVPPSGSNRL
510 520 530 540 550
SSMIGKRTDW CISRQRVWGC PIPVLYNCKT NEPLINDESI NHIKELFGKF
560 570 580 590 600
GSDCWFEMST QQLLPPSLKD QHENFVKGTD TMDVWFDSGT SWRGVLVERG
610 620 630 640 650
IIDKDTGRAD IYLEGSDQHR GWFQSSLLTS VCVRDIEPYK NVVTHGFLLD
660 670 680 690 700
ESGIKMSKSI GNTIVPSTVI KGGPNKVQNP PYGVDLLRTW VASSDYSKDI
710 720 730 740 750
SIGPNILIKI LDGIKKIRNT LRFMLASNFD FDPTIHAIPY EKLSSLDKYA
760 770 780 790 800
LHRVFKLQES VTRHYDQFQF QKVHTEIINF SIEISSFYFD VIKRHLYAES
810 820 830 840 850
PNSHSRRSTQ TVLFKMLDVI NIALAPITVH TSEDVFLHQY QFKNNGKGLD
860 870 880 890 900
KNLIENSVFA HGWDQLPSQY ENELISNQFT NIIAIRDVVN RVLQSMRSQG
910 920 930 940 950
IIGRSDETIM ELTVTNEESS PFYDNLFAIN SQLDDIFCVS NVLLKKFNTF
960 970 980 990 1000
EKDVEQQQQQ QQQQQQQQPQ QIEPNSKGEF IFNTIISNNS KQLGRIEVLL
1010 1020 1030
KISDKFKCPR CWRHTSIEND KVCKPCDSVL NSLK
Length:1,034
Mass (Da):118,422
Last modified:May 24, 2005 - v1
Checksum:iCD81811C34E14FB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000199 Genomic DNA. Translation: EAL60867.1.
RefSeqiXP_629275.1. XM_629273.1.

Genome annotation databases

EnsemblProtistsiDDB0231256; DDB0231256; DDB_G0293030.
GeneIDi8628997.
KEGGiddi:DDB_G0293030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAFI02000199 Genomic DNA. Translation: EAL60867.1.
RefSeqiXP_629275.1. XM_629273.1.

3D structure databases

ProteinModelPortaliQ54CE4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi44689.DDB_0231256.

Proteomic databases

PRIDEiQ54CE4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiDDB0231256; DDB0231256; DDB_G0293030.
GeneIDi8628997.
KEGGiddi:DDB_G0293030.

Organism-specific databases

dictyBaseiDDB_G0293030. mileS.

Phylogenomic databases

eggNOGiCOG0060.
InParanoidiQ54CE4.
KOiK01870.
OMAiQECRKFA.
PhylomeDBiQ54CE4.

Miscellaneous databases

PROiQ54CE4.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiSYIM_DICDI
AccessioniPrimary (citable) accession number: Q54CE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: February 4, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.