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Q54CE4

- SYIM_DICDI

UniProt

Q54CE4 - SYIM_DICDI

Protein

Probable isoleucine--tRNA ligase, mitochondrial

Gene

mileS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (24 May 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei658 – 6581ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. isoleucine-tRNA ligase activity Source: dictyBase

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: dictyBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_221378. Mitochondrial tRNA aminoacylation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
    Alternative name(s):
    Isoleucyl-tRNA synthetase
    Short name:
    IleRS
    Gene namesi
    Name:mileS
    ORF Names:DDB_G0293030
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0293030. mileS.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: dictyBase

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3232MitochondrionSequence AnalysisAdd
    BLAST
    Chaini33 – 10341002Probable isoleucine--tRNA ligase, mitochondrialPRO_0000327970Add
    BLAST

    Proteomic databases

    PRIDEiQ54CE4.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0231256.

    Structurei

    3D structure databases

    ProteinModelPortaliQ54CE4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi94 – 10411"HIGH" regionBy similarityAdd
    BLAST
    Motifi655 – 6595"KMSKS" regionBy similarity

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi956 – 97116Poly-GlnAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiEYPEGHI.
    PhylomeDBiQ54CE4.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q54CE4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISLNNSFFN KRVIVNSFNN YKRSFGTKSQ NEESAIVDSN HSFAHTLNLP     50
    KTTFSMKANA ATREPTLLKD PYKLYKWQLE NNKGENWVFH DGPPYANGDL 100
    HMGHALNKIL KDIVNRYKVL KGFKVNYIPG WDCHGLPIEQ QAFKKLKKSS 150
    DMKASDIRKI AGDFARKEIE KQSKGFQEMG ILGDWENPYK TLDYGYEVEQ 200
    IQTFYDMFNK GYIYRGVKPV HWSPSSRTAL ADAELEYNNN HTSKSIFVKF 250
    NVKSLSNHIL NNLPTTSDIN KAEMVISAII WTTTPWTIPA NQAICVNSEM 300
    DYILVKPIES EQYRNEMFII SKERLESLTK SFNIGELKVI LEFKGEQLKG 350
    TITKHPQYDR ESPIITGDHV IEGSGTGLVH TAPGHGVEDF QICQQQYPDL 400
    KVLSPVNDLG CFTDEVGEKF VGLEVLGDGN EAVINDLETI GSLLHKEDYI 450
    HKYPYDWRTK KPIIIRTTLQ WFVGLKNIQK TALQSIERVN MVPPSGSNRL 500
    SSMIGKRTDW CISRQRVWGC PIPVLYNCKT NEPLINDESI NHIKELFGKF 550
    GSDCWFEMST QQLLPPSLKD QHENFVKGTD TMDVWFDSGT SWRGVLVERG 600
    IIDKDTGRAD IYLEGSDQHR GWFQSSLLTS VCVRDIEPYK NVVTHGFLLD 650
    ESGIKMSKSI GNTIVPSTVI KGGPNKVQNP PYGVDLLRTW VASSDYSKDI 700
    SIGPNILIKI LDGIKKIRNT LRFMLASNFD FDPTIHAIPY EKLSSLDKYA 750
    LHRVFKLQES VTRHYDQFQF QKVHTEIINF SIEISSFYFD VIKRHLYAES 800
    PNSHSRRSTQ TVLFKMLDVI NIALAPITVH TSEDVFLHQY QFKNNGKGLD 850
    KNLIENSVFA HGWDQLPSQY ENELISNQFT NIIAIRDVVN RVLQSMRSQG 900
    IIGRSDETIM ELTVTNEESS PFYDNLFAIN SQLDDIFCVS NVLLKKFNTF 950
    EKDVEQQQQQ QQQQQQQQPQ QIEPNSKGEF IFNTIISNNS KQLGRIEVLL 1000
    KISDKFKCPR CWRHTSIEND KVCKPCDSVL NSLK 1034
    Length:1,034
    Mass (Da):118,422
    Last modified:May 24, 2005 - v1
    Checksum:iCD81811C34E14FB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000199 Genomic DNA. Translation: EAL60867.1.
    RefSeqiXP_629275.1. XM_629273.1.

    Genome annotation databases

    EnsemblProtistsiDDB0231256; DDB0231256; DDB_G0293030.
    GeneIDi8628997.
    KEGGiddi:DDB_G0293030.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000199 Genomic DNA. Translation: EAL60867.1 .
    RefSeqi XP_629275.1. XM_629273.1.

    3D structure databases

    ProteinModelPortali Q54CE4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0231256.

    Proteomic databases

    PRIDEi Q54CE4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0231256 ; DDB0231256 ; DDB_G0293030 .
    GeneIDi 8628997.
    KEGGi ddi:DDB_G0293030.

    Organism-specific databases

    dictyBasei DDB_G0293030. mileS.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi EYPEGHI.
    PhylomeDBi Q54CE4.

    Enzyme and pathway databases

    Reactomei REACT_221378. Mitochondrial tRNA aminoacylation.

    Miscellaneous databases

    PROi Q54CE4.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.

    Entry informationi

    Entry nameiSYIM_DICDI
    AccessioniPrimary (citable) accession number: Q54CE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: May 24, 2005
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3