Probable isoleucine--tRNA ligase, mitochondrial precursor - Dictyostelium discoideum (Slime mold)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable isoleucine--tRNA ligase, mitochondrial
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	mileS
ORF Names:	DDB_G0293030

Organism

Dictyostelium discoideum (Slime mold) [Reference proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	1034 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: dictyBase regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from sequence or structural similarity. Source: dictyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from sequence or structural similarity. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 32

32

Mitochondrion Potential

Chain

33 – 1034

1002

Probable isoleucine--tRNA ligase, mitochondrial

PRO_0000327970

Regions

Motif

94 – 104

11

"HIGH" region By similarity

Motif

655 – 659

5

"KMSKS" region By similarity

Compositional bias

956 – 971

16

Poly-Gln

Sites

Binding site

658

1

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q54CE4 [UniParc].

Last modified May 24, 2005. Version 1.
Checksum: CD81811C34E14FB9
Show »

FASTA

1,034

118,422

        10         20         30         40         50         60 
MISLNNSFFN KRVIVNSFNN YKRSFGTKSQ NEESAIVDSN HSFAHTLNLP KTTFSMKANA 

        70         80         90        100        110        120 
ATREPTLLKD PYKLYKWQLE NNKGENWVFH DGPPYANGDL HMGHALNKIL KDIVNRYKVL 

       130        140        150        160        170        180 
KGFKVNYIPG WDCHGLPIEQ QAFKKLKKSS DMKASDIRKI AGDFARKEIE KQSKGFQEMG 

       190        200        210        220        230        240 
ILGDWENPYK TLDYGYEVEQ IQTFYDMFNK GYIYRGVKPV HWSPSSRTAL ADAELEYNNN 

       250        260        270        280        290        300 
HTSKSIFVKF NVKSLSNHIL NNLPTTSDIN KAEMVISAII WTTTPWTIPA NQAICVNSEM 

       310        320        330        340        350        360 
DYILVKPIES EQYRNEMFII SKERLESLTK SFNIGELKVI LEFKGEQLKG TITKHPQYDR 

       370        380        390        400        410        420 
ESPIITGDHV IEGSGTGLVH TAPGHGVEDF QICQQQYPDL KVLSPVNDLG CFTDEVGEKF 

       430        440        450        460        470        480 
VGLEVLGDGN EAVINDLETI GSLLHKEDYI HKYPYDWRTK KPIIIRTTLQ WFVGLKNIQK 

       490        500        510        520        530        540 
TALQSIERVN MVPPSGSNRL SSMIGKRTDW CISRQRVWGC PIPVLYNCKT NEPLINDESI 

       550        560        570        580        590        600 
NHIKELFGKF GSDCWFEMST QQLLPPSLKD QHENFVKGTD TMDVWFDSGT SWRGVLVERG 

       610        620        630        640        650        660 
IIDKDTGRAD IYLEGSDQHR GWFQSSLLTS VCVRDIEPYK NVVTHGFLLD ESGIKMSKSI 

       670        680        690        700        710        720 
GNTIVPSTVI KGGPNKVQNP PYGVDLLRTW VASSDYSKDI SIGPNILIKI LDGIKKIRNT 

       730        740        750        760        770        780 
LRFMLASNFD FDPTIHAIPY EKLSSLDKYA LHRVFKLQES VTRHYDQFQF QKVHTEIINF 

       790        800        810        820        830        840 
SIEISSFYFD VIKRHLYAES PNSHSRRSTQ TVLFKMLDVI NIALAPITVH TSEDVFLHQY 

       850        860        870        880        890        900 
QFKNNGKGLD KNLIENSVFA HGWDQLPSQY ENELISNQFT NIIAIRDVVN RVLQSMRSQG 

       910        920        930        940        950        960 
IIGRSDETIM ELTVTNEESS PFYDNLFAIN SQLDDIFCVS NVLLKKFNTF EKDVEQQQQQ 

       970        980        990       1000       1010       1020 
QQQQQQQQPQ QIEPNSKGEF IFNTIISNNS KQLGRIEVLL KISDKFKCPR CWRHTSIEND 

      1030 
KVCKPCDSVL NSLK

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References

[1]

"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.

Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFI02000199 Genomic DNA. Translation: EAL60867.1.
RefSeq	XP_629275.1. XM_629273.1.
3D structure databases
HSSP	HSSP built from PDB template 1QU3 based on UniProtKB P41972.
ProteinModelPortal	Q54CE4.
ModBase	Search...
Protein-protein interaction databases
STRING	44689.DDB_0231256.
Proteomic databases
PRIDE	Q54CE4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0231256; DDB0231256; DDB_G0293030.
GeneID	8628997.
KEGG	ddi:DDB_G0293030.
Organism-specific databases
dictyBase	DDB_G0293030. mileS.
Phylogenomic databases
eggNOG	COG0060.
KO	K01870.
OMA	KQVLTHG.
ProtClustDB	CLSZ2429312.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 2 hits.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYIM_DICDI

Accession

Primary (citable) accession number: Q54CE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	May 24, 2005
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents