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Q54CE4

- SYIM_DICDI

UniProt

Q54CE4 - SYIM_DICDI

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Protein
Probable isoleucine--tRNA ligase, mitochondrial
Gene
mileS, DDB_G0293030
Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei658 – 6581ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: dictyBase

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_221378. Mitochondrial tRNA aminoacylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable isoleucine--tRNA ligase, mitochondrial (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:mileS
ORF Names:DDB_G0293030
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 6, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0293030. mileS.

Subcellular locationi

Mitochondrion matrix By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: dictyBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232Mitochondrion Reviewed prediction
Add
BLAST
Chaini33 – 10341002Probable isoleucine--tRNA ligase, mitochondrialUniRule annotation
PRO_0000327970Add
BLAST

Proteomic databases

PRIDEiQ54CE4.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0231256.

Structurei

3D structure databases

ProteinModelPortaliQ54CE4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi94 – 10411"HIGH" region By similarity
Add
BLAST
Motifi655 – 6595"KMSKS" region By similarity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi956 – 97116Poly-GlnUniRule annotation
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiEYPEGHI.
PhylomeDBiQ54CE4.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q54CE4-1 [UniParc]FASTAAdd to Basket

« Hide

MISLNNSFFN KRVIVNSFNN YKRSFGTKSQ NEESAIVDSN HSFAHTLNLP     50
KTTFSMKANA ATREPTLLKD PYKLYKWQLE NNKGENWVFH DGPPYANGDL 100
HMGHALNKIL KDIVNRYKVL KGFKVNYIPG WDCHGLPIEQ QAFKKLKKSS 150
DMKASDIRKI AGDFARKEIE KQSKGFQEMG ILGDWENPYK TLDYGYEVEQ 200
IQTFYDMFNK GYIYRGVKPV HWSPSSRTAL ADAELEYNNN HTSKSIFVKF 250
NVKSLSNHIL NNLPTTSDIN KAEMVISAII WTTTPWTIPA NQAICVNSEM 300
DYILVKPIES EQYRNEMFII SKERLESLTK SFNIGELKVI LEFKGEQLKG 350
TITKHPQYDR ESPIITGDHV IEGSGTGLVH TAPGHGVEDF QICQQQYPDL 400
KVLSPVNDLG CFTDEVGEKF VGLEVLGDGN EAVINDLETI GSLLHKEDYI 450
HKYPYDWRTK KPIIIRTTLQ WFVGLKNIQK TALQSIERVN MVPPSGSNRL 500
SSMIGKRTDW CISRQRVWGC PIPVLYNCKT NEPLINDESI NHIKELFGKF 550
GSDCWFEMST QQLLPPSLKD QHENFVKGTD TMDVWFDSGT SWRGVLVERG 600
IIDKDTGRAD IYLEGSDQHR GWFQSSLLTS VCVRDIEPYK NVVTHGFLLD 650
ESGIKMSKSI GNTIVPSTVI KGGPNKVQNP PYGVDLLRTW VASSDYSKDI 700
SIGPNILIKI LDGIKKIRNT LRFMLASNFD FDPTIHAIPY EKLSSLDKYA 750
LHRVFKLQES VTRHYDQFQF QKVHTEIINF SIEISSFYFD VIKRHLYAES 800
PNSHSRRSTQ TVLFKMLDVI NIALAPITVH TSEDVFLHQY QFKNNGKGLD 850
KNLIENSVFA HGWDQLPSQY ENELISNQFT NIIAIRDVVN RVLQSMRSQG 900
IIGRSDETIM ELTVTNEESS PFYDNLFAIN SQLDDIFCVS NVLLKKFNTF 950
EKDVEQQQQQ QQQQQQQQPQ QIEPNSKGEF IFNTIISNNS KQLGRIEVLL 1000
KISDKFKCPR CWRHTSIEND KVCKPCDSVL NSLK 1034
Length:1,034
Mass (Da):118,422
Last modified:May 24, 2005 - v1
Checksum:iCD81811C34E14FB9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000199 Genomic DNA. Translation: EAL60867.1.
RefSeqiXP_629275.1. XM_629273.1.

Genome annotation databases

EnsemblProtistsiDDB0231256; DDB0231256; DDB_G0293030.
GeneIDi8628997.
KEGGiddi:DDB_G0293030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000199 Genomic DNA. Translation: EAL60867.1 .
RefSeqi XP_629275.1. XM_629273.1.

3D structure databases

ProteinModelPortali Q54CE4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0231256.

Proteomic databases

PRIDEi Q54CE4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0231256 ; DDB0231256 ; DDB_G0293030 .
GeneIDi 8628997.
KEGGi ddi:DDB_G0293030.

Organism-specific databases

dictyBasei DDB_G0293030. mileS.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi EYPEGHI.
PhylomeDBi Q54CE4.

Enzyme and pathway databases

Reactomei REACT_221378. Mitochondrial tRNA aminoacylation.

Miscellaneous databases

PROi Q54CE4.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.

Entry informationi

Entry nameiSYIM_DICDI
AccessioniPrimary (citable) accession number: Q54CE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: May 24, 2005
Last modified: September 3, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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